BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0251.0001.p (198 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_662037.1| conserved hypothetical protein [Chlorobium tepi... 77 2e-13 ref|NP_699615.1| Bme2 protein [Brucella suis 1330] >gi|23463775|... 70 3e-11 gb|AAD43833.1| Bme2 [Brucella melitensis] 70 3e-11 ref|NP_541819.1| hypothetical protein [Brucella melitensis] >gi|... 70 4e-11 ref|NP_355852.1| AGR_L_144p [Agrobacterium tumefaciens] >gi|2551... 69 5e-11 >ref|NP_662037.1| conserved hypothetical protein [Chlorobium tepidum TLS] gb|AAM72379.1| conserved hypothetical protein [Chlorobium tepidum TLS] Length = 196 Score = 77.0 bits (188), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 52/171 (30%), Positives = 89/171 (52%), Gaps = 6/171 (3%) Query: 17 GAGRLLLIHQA-PRQADIIIVLSGGAG-RSEKGAELYKEGYTQNILLS--NAKESASNSG 72 G G L+ +H P +ADII++L G G R KG +LYK GY +++LL+ +++ N Sbjct: 25 GLGFLVSMHSTKPEKADIIVILGGDDGLRVSKGGDLYKAGYAKHVLLTGIDSRYYRPNHP 84 Query: 73 DMLQTAL-ALGIPKDAILTEKAALSTYQNAKLTLPIMKQHGFTSAIVVSSDFHMRRAKIL 131 + + L A G+P+ I+ + + ++++ A+ T +M ++G+ SA+VVS HM R Sbjct: 85 NWRERKLMARGVPRKHIIVDTWSETSWEEAENTSDLMDKNGWKSALVVSDPPHMLRLNKT 144 Query: 132 FDHVYKHSGIELTYVGSD-SGYNAKRWWSDRYSRETTFNEYTKMIGNLFGY 181 + + + V ++ S +N WW + S NE K I L Y Sbjct: 145 WKKAFAGTNKRFRLVATEPSWWNPLLWWRNPISYRFVINELKKNIYYLVTY 195 >ref|NP_699615.1| Bme2 protein [Brucella suis 1330] gb|AAN33620.1| Bme2 protein [Brucella suis 1330] Length = 205 Score = 69.7 bits (169), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 44/116 (37%), Positives = 67/116 (57%), Gaps = 11/116 (9%) Query: 21 LLLIHQAPRQADIIIVLSG-GAGRSEKGAELYKEGYTQNILLSNAKESASNSGDML---Q 76 LL + R AD+IIV G GA R+ + A+L+K+G + IL A+ GD L Sbjct: 23 LLTLQDDGRPADVIIVPGGDGAPRAAQAAQLWKDGRSPYIL-------ATGDGDCLLNKH 75 Query: 77 TALALGIPKDAILTEKAALSTYQNAKLTLPIMKQHGFTSAIVVSSDFHMRRAKILF 132 +A G+P AIL E + ST+QNA+ + P++++ SA++V++ FH RRA F Sbjct: 76 IMMAKGVPPSAILVECRSGSTWQNARFSAPLLRRLHAKSALIVTNWFHSRRAVASF 131 >gb|AAD43833.1| Bme2 [Brucella melitensis] Length = 205 Score = 69.7 bits (169), Expect = 3e-11, Method: Compositional matrix adjust. Identities = 44/116 (37%), Positives = 67/116 (57%), Gaps = 11/116 (9%) Query: 21 LLLIHQAPRQADIIIVLSG-GAGRSEKGAELYKEGYTQNILLSNAKESASNSGDML---Q 76 LL + R AD+IIV G GA R+ + A+L+K+G + IL A+ GD L Sbjct: 23 LLTLQDDGRPADVIIVPGGDGAPRAAQAAQLWKDGRSPYIL-------ATGDGDCLLNKH 75 Query: 77 TALALGIPKDAILTEKAALSTYQNAKLTLPIMKQHGFTSAIVVSSDFHMRRAKILF 132 +A G+P AIL E + ST+QNA+ + P++++ SA++V++ FH RRA F Sbjct: 76 IMMAKGVPPSAILVECRSGSTWQNARFSAPLLRRLHAKSALIVTNWFHSRRAVASF 131 >ref|NP_541819.1| hypothetical protein [Brucella melitensis] pir||AH3614 hypothetical protein BMEII0841 [imported] - Brucella melitensis (strain 16M) gb|AAL54083.1| hypothetical protein [Brucella melitensis 16M] Length = 180 Score = 69.7 bits (169), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 42/108 (38%), Positives = 64/108 (59%), Gaps = 11/108 (10%) Query: 29 RQADIIIVLSG-GAGRSEKGAELYKEGYTQNILLSNAKESASNSGDML---QTALALGIP 84 R AD+IIV G GA R+ + A+L+K+G + IL A+ GD L +A G+P Sbjct: 6 RPADVIIVPGGDGAPRAAQAAQLWKDGRSPYIL-------ATGDGDCLLNKHIMMAKGVP 58 Query: 85 KDAILTEKAALSTYQNAKLTLPIMKQHGFTSAIVVSSDFHMRRAKILF 132 AIL E + ST+QNA+ + P++++ SA++V++ FH RRA F Sbjct: 59 PSAILVECRSGSTWQNARFSAPLLRRLHAKSALIVTNWFHSRRAVASF 106 >ref|NP_355852.1| AGR_L_144p [Agrobacterium tumefaciens] pir||C98139 bme2 protein (AF076290) [imported] - Agrobacterium tumefaciens (strain C58, Cereon) gb|AAK88637.1| AGR_L_144p [Agrobacterium tumefaciens str. C58 (Cereon)] Length = 252 Score = 69.3 bits (168), Expect = 5e-11, Method: Compositional matrix adjust. Identities = 44/123 (35%), Positives = 66/123 (53%), Gaps = 7/123 (5%) Query: 18 AGRLLLIHQAPRQADIIIVLSG-GAGRSEKGAELYKEGYTQNILLSNAKESASNSGDMLQ 76 AG LL++ P QAD+I+VL G G R+ + A+L+ EG +L++ + D ++ Sbjct: 80 AGSLLVVEDPPAQADVIVVLGGDGPPRAAQAAKLWHEGKAPLVLVTGYGDC-----DFIR 134 Query: 77 TALAL-GIPKDAILTEKAALSTYQNAKLTLPIMKQHGFTSAIVVSSDFHMRRAKILFDHV 135 L G+ A+ TE +LST++NA PI+ G AI+V+S FH RRA F V Sbjct: 135 DMLVRSGVDFAAVSTECLSLSTWENATFAQPILTGMGAKRAILVTSWFHSRRAVKRFRFV 194 Query: 136 YKH 138 Sbjct: 195 MPQ 197 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 262,164,156 Number of extensions: 10029143 Number of successful extensions: 35518 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 35551 Number of HSP's successfully gapped: 35 Length of query: 198 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 79 Effective length of database: 434,140,797 Effective search space: 34297122963 Effective search space used: 34297122963 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 123 (52.0 bits)