BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0008.p (198 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00186896.1| hypothetical protein Rxyl219501 [Rubrobacter ... 123 2e-27 ref|NP_691441.1| transcriptional regulator [Oceanobacillus iheye... 67 2e-10 ref|NP_981289.1| transcriptional regulator, TetR family domain p... 66 4e-10 ref|ZP_00088055.1| COG1309: Transcriptional regulator [Pseudomon... 63 4e-09 ref|NP_243968.1| transcriptional regulator (TetR/AcrR family) [B... 61 1e-08 >ref|ZP_00186896.1| hypothetical protein Rxyl219501 [Rubrobacter xylanophilus DSM 9941] Length = 221 Score = 123 bits (309), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 64/190 (33%), Positives = 111/190 (58%), Gaps = 6/190 (3%) Query: 10 KRKQILFAAFQALSELGYDSVTLQTIADHAEVSKGVVHYYFDNKEAVLVELLEWLTAKIS 69 K+++++ +A++ + E G ++++LQ +AD A VSK ++ YYF +KE ++V + W+ A+++ Sbjct: 20 KQERLVRSAYKVMGEKGMNNLSLQDVADEAGVSKAILPYYFASKENLMVITMRWVLARVA 79 Query: 70 XXXXXXXXXXXXXXGKLKAYIDSAFPGPAKNRSFYRVYLDFLARASRIPVYREINQRFYD 129 K+ A +D+ F PA NRSFY V+ DFL A+R + ++ F + Sbjct: 80 RRIREAVSRAEGAEEKVSAMVDAIFIDPASNRSFYLVFFDFLGHAARTDRFGDVGATFQE 139 Query: 130 NCARISTEVLTLGQKEGIFAKELAPDT--TAPVIRAIIDGCLIQWLMKDD-DELHAAYKE 186 + EV+ LG++EG+F DT A V+RA+IDG QW+++ D + HA Y+ Sbjct: 140 IVTGLYDEVVRLGREEGVF---RVGDTREAATVVRALIDGLFTQWVLERDWERTHARYRG 196 Query: 187 MCYEAVMKVL 196 +C AV+ L Sbjct: 197 LCKRAVLSYL 206 >ref|NP_691441.1| transcriptional regulator [Oceanobacillus iheyensis HTE831] dbj|BAC12476.1| transcriptional regulator (TetR/AcrR family) [Oceanobacillus iheyensis HTE831] Length = 203 Score = 67.0 bits (162), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 44/184 (23%), Positives = 91/184 (49%), Gaps = 1/184 (0%) Query: 5 KKSEEKRKQILFAAFQALSELGYDSVTLQTIADHAEVSKGVVHYYFDNKEAVLVELLEWL 64 ++ E +R+ +L +A + S GY + T+ I ++ +SKG V+ YF +KE + + LL+ Sbjct: 8 EEKEARREHLLESALECFSAKGYYASTVDDIVRYSNLSKGSVYNYFKSKEEIFIHLLQKK 67 Query: 65 TAKISXXXXXXXXXXXXXXGKLKAYIDSAFPGPAKNRSFYRVYLDFLARASRIPVYREIN 124 ++ KLK +I P + + F RV+++F ++ P + I Sbjct: 68 RQEMVEELTIKLAKIDSPLEKLKYWIREDIPYSLEKKKFMRVHVEFWLYSTDSPEVQHIL 127 Query: 125 QRFYDNCARISTEVLTLGQKEGIFAKELAPDTTAPVIRAIIDGCLIQWLMK-DDDELHAA 183 +D+ ++ E++ GQK G ++ + + + ++ DG + ++ D+ +L A+ Sbjct: 128 VERFDDMFGLTKEIIEQGQKLGEIKPDIDAEAASSMFWSLHDGIWLHAVIGYDEAKLEAS 187 Query: 184 YKEM 187 KEM Sbjct: 188 IKEM 191 >ref|NP_981289.1| transcriptional regulator, TetR family domain protein [Bacillus cereus ATCC 10987] gb|AAS43897.1| transcriptional regulator, TetR family domain protein [Bacillus cereus ATCC 10987] Length = 197 Score = 66.2 bits (160), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 43/163 (26%), Positives = 79/163 (48%), Gaps = 3/163 (1%) Query: 7 SEEKRKQILFAAFQALSELGYDSVTLQTIADHAEVSKGVVHYYFDNKEAVLVELLEWLTA 66 +E +R+QI+ A L E+GY++++L +A A++S G++ Y+F++K ++ L++L Sbjct: 7 AEARREQIILACIDTLEEVGYNNLSLTKVAKKAKISTGLISYHFNDKLYLMNRTLQFLVE 66 Query: 67 KISXXXXXXXXXXXXXXGKLKAYIDS--AFPGPAKNRSFYRVYLDFLARA-SRIPVYREI 123 K KL+A+I++ A+ + + + F AR IP YR Sbjct: 67 KQHEFISNRVLLAQSEINKLEAFIEAHLAYQETHCKNNIALIEIVFNARNEENIPYYRIE 126 Query: 124 NQRFYDNCARISTEVLTLGQKEGIFAKELAPDTTAPVIRAIID 166 + D + +L GQ+ G+F+ DT A I I+ Sbjct: 127 DDEEDDALRTMLLNILKTGQQNGVFSNSFQADTLASFILGAIE 169 >ref|ZP_00088055.1| COG1309: Transcriptional regulator [Pseudomonas fluorescens PfO-1] Length = 220 Score = 62.8 bits (151), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 45/167 (26%), Positives = 76/167 (45%), Gaps = 10/167 (5%) Query: 9 EKRKQILFAAFQALSELGYDSVTLQTIADHAEVSKGVVHYYFDNKEAVLVELLEWLTAKI 68 EKR ++L AA + +++ GY +L+ +A HA + G V YYF NKE ++ + L + Sbjct: 34 EKRGELLTAAIEVIAQEGYAGTSLRKVASHAGCTTGAVTYYFANKEEMITAAAQSLFDEF 93 Query: 69 SXXXXXXXXXXXXXXGKLKAYIDSAFP-GPAKNRSFYRVYLDFLARASRIPVYREINQRF 127 ++A I+ A + + + LA A PV+ ++ Q+ Sbjct: 94 DHLLDAGQEKV-----DVRAMIEQWLNLTQANDSARWLALFQMLAHARHEPVFADVIQKR 148 Query: 128 YDNCARISTEVLTLGQKEGIFAKELAPDTTAPVIRAIIDGCLIQWLM 174 Y +L GQ +GI K++A D A + A+ DG W+M Sbjct: 149 YSRFREDLASILRKGQSQGIIRKDIAADLLADQLSALSDG----WMM 191 >ref|NP_243968.1| transcriptional regulator (TetR/AcrR family) [Bacillus halodurans] pir||F84037 transcription regulator (TetR/AcrR family) BH3102 [imported] - Bacillus halodurans (strain C-125) dbj|BAB06821.1| transcriptional regulator (TetR/AcrR family) [Bacillus halodurans] Length = 195 Score = 60.8 bits (146), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 48/174 (27%), Positives = 77/174 (44%), Gaps = 6/174 (3%) Query: 5 KKSEEKRKQILFAAFQALSELGYDSVTLQTIADHAEVSKGVVHYYFDNKEAVLVELLEWL 64 KK K QI+ AA Q ++E GY + IA A V+ G ++ YF+NKE VL+ L + Sbjct: 3 KKKGPKYDQIIDAAVQVIAEHGYHQAQVSKIAKAAGVADGTIYLYFNNKEDVLISLFQEK 62 Query: 65 TAKISXXXXXXXXXXXXXXGKLKAYIDSAFPGPAKNRSFYRVYLDFLARASRIPVYREIN 124 + KLK ++ F A + + R S + +IN Sbjct: 63 MGRFVDKIRSQMNEATDVEEKLKILVNMHFKQLAADHKL-AIVTQLELRQSNTELRLKIN 121 Query: 125 Q--RFYDNCARISTEVLTLGQKEGIFAKELAPDTTAPVIRAIIDGCLIQWLMKD 176 + + Y N + E+L G+++G F +EL +I +D + W+MKD Sbjct: 122 EVLKGYLN---LLDELLMEGKEKGYFFQELDTRLARQMIFGTLDEVVTNWVMKD 172 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 250,776,023 Number of extensions: 8320063 Number of successful extensions: 19832 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 19855 Number of HSP's successfully gapped: 29 Length of query: 198 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 79 Effective length of database: 434,140,797 Effective search space: 34297122963 Effective search space used: 34297122963 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 123 (52.0 bits)