BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0250.0005.p (88 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_349082.1| Predicted acetyltransferase [Clostridium acetob... 66 2e-10 gb|EAK56664.1| unknown [environmental sequence] 64 1e-09 ref|NP_902622.1| conserved hypothetical protein [Chromobacterium... 63 2e-09 gb|EAJ61628.1| unknown [environmental sequence] 63 2e-09 gb|EAH37692.1| unknown [environmental sequence] 63 2e-09 >ref|NP_349082.1| Predicted acetyltransferase [Clostridium acetobutylicum] pir||C97204 probable acetyltransferase [imported] - Clostridium acetobutylicum gb|AAK80422.1| Predicted acetyltransferase [Clostridium acetobutylicum] Length = 140 Score = 66.2 bits (160), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 34/83 (40%), Positives = 56/83 (67%), Gaps = 4/83 (4%) Query: 1 MMGIICQDLVGDCRPFMVIENVVVSSRARRQGLGKKLIAALEAIAHERDCYYIMFVSGEK 60 ++GI C+ L+ PF+VIE+V+V R +G+G+KL+ AL+ A+ ++C Y + VS Sbjct: 59 VLGICCKGLM---FPFLVIEDVIVKEEIRGKGVGRKLMKALDEFANRKNCSYAILVSSGF 115 Query: 61 RKEAHIFYEAMGYREEKVEGYRK 83 R+ AH FYE +G+++ V G+RK Sbjct: 116 REGAHKFYERVGFKDS-VRGFRK 137 >gb|EAK56664.1| unknown [environmental sequence] Length = 163 Score = 63.9 bits (154), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 28/80 (35%), Positives = 52/80 (65%), Gaps = 1/80 (1%) Query: 2 MGIICQDLVGDCRPFMVIENVVVSSRARRQGLGKKLIAALEAIAHERDCYYIMFVSGEKR 61 +G++ L RPF ++E+VV +S RR+G+ ++L+ A+A ++DCY +M +SGE R Sbjct: 64 LGVVA-TLTNGGRPFGMVEHVVTASAFRRRGISRQLLTHALALAWQQDCYKVMLLSGEGR 122 Query: 62 KEAHIFYEAMGYREEKVEGY 81 + AH Y ++G++ +G+ Sbjct: 123 EAAHRLYASLGFKAGIEKGF 142 >ref|NP_902622.1| conserved hypothetical protein [Chromobacterium violaceum ATCC 12472] gb|AAQ60620.1| conserved hypothetical protein [Chromobacterium violaceum ATCC 12472] Length = 161 Score = 63.2 bits (152), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 31/80 (38%), Positives = 48/80 (60%) Query: 5 ICQDLVGDCRPFMVIENVVVSSRARRQGLGKKLIAALEAIAHERDCYYIMFVSGEKRKEA 64 C+D++ +PF V+ENVVV +R R +GLG L+ E + RDC IM +S R++A Sbjct: 72 FCEDVMYGAQPFAVLENVVVDARWRGRGLGAALLREAERRSLARDCSKIMLLSSAGREDA 131 Query: 65 HIFYEAMGYREEKVEGYRKH 84 H F+ G+R + G+ K+ Sbjct: 132 HRFFLRAGFRGDAKRGFVKY 151 >gb|EAJ61628.1| unknown [environmental sequence] Length = 149 Score = 63.2 bits (152), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 28/83 (33%), Positives = 50/83 (60%) Query: 5 ICQDLVGDCRPFMVIENVVVSSRARRQGLGKKLIAALEAIAHERDCYYIMFVSGEKRKEA 64 +C D++ +PF V+ENV+V + AR G+G L A+EA + +C IM +S +R ++ Sbjct: 62 LCSDVMFRQQPFAVVENVIVDAVARGLGVGSTLFKAIEAFCFKSNCSKIMLLSSVQRSDS 121 Query: 65 HIFYEAMGYREEKVEGYRKHLSS 87 H ++E G++ G+ K+ S+ Sbjct: 122 HTYFEKQGFKGSVKRGFVKYRSA 144 >gb|EAH37692.1| unknown [environmental sequence] Length = 126 Score = 63.2 bits (152), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 29/77 (37%), Positives = 45/77 (58%) Query: 5 ICQDLVGDCRPFMVIENVVVSSRARRQGLGKKLIAALEAIAHERDCYYIMFVSGEKRKEA 64 IC L +PF +IE+V+ + RR+GL +K+I IA ++CY +M +SGE R A Sbjct: 43 ICPTLTNAAKPFGIIEHVITAEAYRRRGLSEKVIERALDIAWRQNCYKVMLLSGESRTAA 102 Query: 65 HIFYEAMGYREEKVEGY 81 H YE +G++ G+ Sbjct: 103 HKLYEKIGFQSGIERGF 119 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.139 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 120,550,525 Number of extensions: 3779331 Number of successful extensions: 10755 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 10769 Number of HSP's successfully gapped: 10 Length of query: 88 Length of database: 757,237,578 Length adjustment: 57 Effective length of query: 31 Effective length of database: 602,476,935 Effective search space: 18676784985 Effective search space used: 18676784985 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 120 (50.8 bits)