BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0030.p (323 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_388821.1| spoVR [Bacillus subtilis] >gi|586024|sp|P37875|... 383 e-105 gb|AAA22811.1| spoVR 382 e-105 ref|NP_693567.1| stage V sporulation protein R [Oceanobacillus i... 378 e-103 ref|NP_977176.1| stage V sporulation protein R [Bacillus cereus ... 375 e-103 ref|NP_241978.1| involved in spore cortex synthesis [Bacillus ha... 375 e-103 >ref|NP_388821.1| spoVR [Bacillus subtilis] sp|P37875|SP5R_BACSU Stage V sporulation protein R pir||D69716 involved in spore cortex synthesis spoVR - Bacillus subtilis emb|CAA74485.1| spoVR [Bacillus subtilis] emb|CAB12779.1| spoVR [Bacillus subtilis subsp. subtilis str. 168] Length = 468 Score = 383 bits (984), Expect = e-105, Method: Compositional matrix adjust. Identities = 193/369 (52%), Positives = 241/369 (65%), Gaps = 50/369 (13%) Query: 1 MTDNETGQLEESVEHLTGLALEHGLDFFSMRYQMCPPEVLYSIGAYGMPTRFAHWSFGKA 60 M E L+ ++E +T +A GLDF+ MRY++CP E++Y+ GAYGMPTRF+HWSFGK Sbjct: 1 MNAEEKKGLQRAIEEITEIAKGFGLDFYPMRYEICPAEIIYTFGAYGMPTRFSHWSFGKQ 60 Query: 61 YHRMKSEYDHGLSKIYELVINSDPCYAFLLDSNTLLQNKLIVAHVLGHSDFFKNNAMFGG 120 +H+MK YD GLSKIYELVINSDPCYAFLLDSN+L+QNKLIVAHVL H DFFKNN F Sbjct: 61 FHKMKLHYDLGLSKIYELVINSDPCYAFLLDSNSLIQNKLIVAHVLAHCDFFKNNCRFQN 120 Query: 121 TDRNMINRMAIFAERIDRFGQEYGTDEVERFLDAGIAIQEHIDPFSARSGRPGGFREIGG 180 T R+M+ MA AERI + Q +G EVE FLDA ++IQEHIDP R + Sbjct: 121 TKRDMVESMAATAERIKHYEQVHGIKEVESFLDAILSIQEHIDPSLVRPKLQWSVDDEEE 180 Query: 181 EV----------------------------------KDVIGYIAAESRYLEEWQREILYM 206 E+ KD++ +I SR LE WQR+IL M Sbjct: 181 EIEEAATPYDDLWSLDEKKPKKQVKKSKKPFPPRPEKDILLFIEEHSRELEPWQRDILTM 240 Query: 207 LRAEMLYFWPQMETKIMNEGWASYWHLKLVRELDLSDSEIIEFSKMNSGVIQPSPGRINP 266 +R EMLYFWPQ+ETKIMNEGWASYWH +++RELDL+ E IEF+K+N+GV+QPS INP Sbjct: 241 MREEMLYFWPQLETKIMNEGWASYWHQRIIRELDLTSDEAIEFAKLNAGVVQPSKTGINP 300 Query: 267 YYLGLMLLRHIEKR----------------EGLQALFEIREIESDVSFIRNYLTRELAEE 310 YYLGL + IEKR G + +FE+REIESD+SFIRNYLT+EL Sbjct: 301 YYLGLKIFEDIEKRYNNPTEEMKKMGVQPDSGREKMFEVREIESDISFIRNYLTKELVMR 360 Query: 311 MDLFVFKKE 319 DL++F+K+ Sbjct: 361 EDLYLFQKQ 369 >gb|AAA22811.1| spoVR Length = 468 Score = 382 bits (980), Expect = e-105, Method: Compositional matrix adjust. Identities = 192/365 (52%), Positives = 240/365 (65%), Gaps = 50/365 (13%) Query: 5 ETGQLEESVEHLTGLALEHGLDFFSMRYQMCPPEVLYSIGAYGMPTRFAHWSFGKAYHRM 64 E L+ ++E +T +A GLDF+ MRY++CP E++Y+ GAYGMPTRF+HWSFGK +H+M Sbjct: 5 EKKGLQRAIEEITEIAKGFGLDFYPMRYEICPAEIIYTFGAYGMPTRFSHWSFGKQFHKM 64 Query: 65 KSEYDHGLSKIYELVINSDPCYAFLLDSNTLLQNKLIVAHVLGHSDFFKNNAMFGGTDRN 124 K YD GLSKIYELVINSDPCYAFLLDSN+L+QNKLIVAHVL H DFFKNN F T R+ Sbjct: 65 KLHYDLGLSKIYELVINSDPCYAFLLDSNSLIQNKLIVAHVLAHCDFFKNNCRFQNTKRD 124 Query: 125 MINRMAIFAERIDRFGQEYGTDEVERFLDAGIAIQEHIDPFSARSGRPGGFREIGGEV-- 182 M+ MA AERI + Q +G EVE FLDA ++IQEHIDP R + E+ Sbjct: 125 MVESMAATAERIKHYEQVHGIKEVESFLDAILSIQEHIDPSLVRPKLQWSVDDEEEEIEE 184 Query: 183 --------------------------------KDVIGYIAAESRYLEEWQREILYMLRAE 210 KD++ +I SR LE WQR+IL M+R E Sbjct: 185 AATPYDDLWSLDEKKPKKQVKKSKKPFPPRPEKDILLFIEEHSRELEPWQRDILTMMREE 244 Query: 211 MLYFWPQMETKIMNEGWASYWHLKLVRELDLSDSEIIEFSKMNSGVIQPSPGRINPYYLG 270 MLYFWPQ+ETKIMNEGWASYWH +++RELDL+ E IEF+K+N+GV+QPS INPYYLG Sbjct: 245 MLYFWPQLETKIMNEGWASYWHQRIIRELDLTSDEAIEFAKLNAGVVQPSKTGINPYYLG 304 Query: 271 LMLLRHIEKR----------------EGLQALFEIREIESDVSFIRNYLTRELAEEMDLF 314 L + IEKR G + +FE+REIESD+SFIRNYLT+EL DL+ Sbjct: 305 LKIFEDIEKRYNNPTEEMKKMGVQPDSGREKMFEVREIESDISFIRNYLTKELVMREDLY 364 Query: 315 VFKKE 319 +F+K+ Sbjct: 365 LFQKQ 369 >ref|NP_693567.1| stage V sporulation protein R [Oceanobacillus iheyensis HTE831] dbj|BAC14602.1| stage V sporulation protein R (spore cortex synthesis) [Oceanobacillus iheyensis HTE831] Length = 470 Score = 378 bits (970), Expect = e-103, Method: Compositional matrix adjust. Identities = 185/369 (50%), Positives = 244/369 (66%), Gaps = 53/369 (14%) Query: 4 NETGQLEESVEHLTGLALEHGLDFFSMRYQMCPPEVLYSIGAYGMPTRFAHWSFGKAYHR 63 ++T +L +++ +T +A GLDF+ MRY++CP +++Y+ GAYGMPTRF HWSFGK +H+ Sbjct: 3 DQTKELSYAIDEITEVAKGFGLDFYPMRYEICPADIIYTFGAYGMPTRFTHWSFGKQFHK 62 Query: 64 MKSEYDHGLSKIYELVINSDPCYAFLLDSNTLLQNKLIVAHVLGHSDFFKNNAMFGGTDR 123 MK +YD GLS+IYELVINSDPCYAFLLD+N+L+QNKLI+AHVL H DFFKNN F T R Sbjct: 63 MKLQYDLGLSQIYELVINSDPCYAFLLDTNSLVQNKLIIAHVLAHCDFFKNNVRFSNTRR 122 Query: 124 NMINRMAIFAERIDRFGQEYGTDEVERFLDAGIAIQEHIDPFSARSGRPGGFREIGGEV- 182 +M+ M AERI + YG DEVE FLDA ++IQEHIDP RS P E + Sbjct: 123 DMVESMTATAERIAHYEMVYGKDEVESFLDAVLSIQEHIDPSIVRSKLPQTEEEDIEDTT 182 Query: 183 ------------------------------------KDVIGYIAAESRYLEEWQREILYM 206 KD++ +I SR LE WQR+IL M Sbjct: 183 LSKVSEYDDLWKLDEDRLKQEPKKRKKMKKIPPQPEKDILLFIEEYSRELEGWQRDILTM 242 Query: 207 LRAEMLYFWPQMETKIMNEGWASYWHLKLVRELDLSDSEIIEFSKMNSGVIQPSPGRINP 266 +R EMLYFWPQ+ETKIMNEGWASYWH +++RELDL+ +E IE+SK+N+GV+QPS INP Sbjct: 243 MREEMLYFWPQLETKIMNEGWASYWHQRIMRELDLTTNETIEYSKLNAGVVQPSRTSINP 302 Query: 267 YYLGLMLLRHIEKRE----------------GLQALFEIREIESDVSFIRNYLTRELAEE 310 YYLG+ + IE+R G + +FE+REIESD+SFIRNYLT++L + Sbjct: 303 YYLGIKIFEDIEERYNHPTKEMIENGYKPNGGREKIFEVREIESDISFIRNYLTKDLVAQ 362 Query: 311 MDLFVFKKE 319 D+++F+K+ Sbjct: 363 EDMYLFEKK 371 >ref|NP_977176.1| stage V sporulation protein R [Bacillus cereus ATCC 10987] gb|AAS39784.1| stage V sporulation protein R [Bacillus cereus ATCC 10987] Length = 470 Score = 375 bits (963), Expect = e-103, Method: Compositional matrix adjust. Identities = 186/362 (51%), Positives = 240/362 (66%), Gaps = 54/362 (14%) Query: 12 SVEHLTGLALEHGLDFFSMRYQMCPPEVLYSIGAYGMPTRFAHWSFGKAYHRMKSEYDHG 71 +++ +T +A GLDF+ MRY++CP E++Y+ GAYGMPTRF+HWSFGK + +MK YD G Sbjct: 10 AIDEITEIATGFGLDFYPMRYEVCPAEIIYTFGAYGMPTRFSHWSFGKQFFKMKLHYDLG 69 Query: 72 LSKIYELVINSDPCYAFLLDSNTLLQNKLIVAHVLGHSDFFKNNAMFGGTDRNMINRMAI 131 LSKIYELVINS+PCYAFLLD+N+L+QNKLIVAHVL H DFFKNN F T R+M+ MA Sbjct: 70 LSKIYELVINSNPCYAFLLDTNSLIQNKLIVAHVLAHCDFFKNNIRFSNTKRDMVESMAA 129 Query: 132 FAERIDRFGQEYGTDEVERFLDAGIAIQEHIDPFSARS----------------GRPGGF 175 AERI + EYG EVE+FLDA +AIQEHIDP R+ R + Sbjct: 130 TAERIKAYEHEYGRQEVEKFLDAVLAIQEHIDPSLVRNKLAWTIDDMYVEEKEETRKTVY 189 Query: 176 REIGG----------------------EVKDVIGYIAAESRYLEEWQREILYMLRAEMLY 213 ++ G KD++ +I SR LEEWQR+IL MLR EMLY Sbjct: 190 DDLWGLDDAKKKRESPLIRKKKKFPPQPEKDLLLFIEEYSRELEEWQRDILTMLREEMLY 249 Query: 214 FWPQMETKIMNEGWASYWHLKLVRELDLSDSEIIEFSKMNSGVIQPSPGRINPYYLGLML 273 FW QMETKIMNEGWAS WH +++RE+DL+ SE IEF+K+N+GV+QPS INPYYLG+ + Sbjct: 250 FWAQMETKIMNEGWASLWHQRILREMDLTSSEAIEFAKLNAGVVQPSKTSINPYYLGIKM 309 Query: 274 LRHIEKR----------------EGLQALFEIREIESDVSFIRNYLTRELAEEMDLFVFK 317 HIE+R G Q +FE+RE++SD SFIRNYLT+EL D+++F+ Sbjct: 310 FEHIEERYDNPTEEMKRRGIKPGSGKQKIFEVREMDSDTSFIRNYLTKELVMREDMYLFQ 369 Query: 318 KE 319 ++ Sbjct: 370 RQ 371 >ref|NP_241978.1| involved in spore cortex synthesis [Bacillus halodurans] pir||H83788 involved in spore cortex synthesis spoVR [imported] - Bacillus halodurans (strain C-125) dbj|BAB04831.1| involved in spore cortex synthesis [Bacillus halodurans] Length = 411 Score = 375 bits (962), Expect = e-103, Method: Compositional matrix adjust. Identities = 185/370 (50%), Positives = 248/370 (67%), Gaps = 51/370 (13%) Query: 1 MTDNETGQLEESVEHLTGLALEHGLDFFSMRYQMCPPEVLYSIGAYGMPTRFAHWSFGKA 60 M+ + +L+ ++E +T +A GLDF+ MRY++CP +++Y+ GAYGMPTR+ HWSFGK Sbjct: 1 MSTDAWKELQHAIEEITEIAEGFGLDFYPMRYEICPADIIYTFGAYGMPTRYTHWSFGKQ 60 Query: 61 YHRMKSEYDHGLSKIYELVINSDPCYAFLLDSNTLLQNKLIVAHVLGHSDFFKNNAMFGG 120 +++MK +YD GLSKIYELVINSDPCYAFLLDSN+L+QNKLIVAHVL H DFFKNN F Sbjct: 61 FYKMKLQYDLGLSKIYELVINSDPCYAFLLDSNSLIQNKLIVAHVLAHCDFFKNNVRFSN 120 Query: 121 TDRNMINRMAIFAERIDRFGQEYGTDEVERFLDAGIAIQEHIDPFSAR------------ 168 T R+M+ M+ AERI + +G EVERFLDA +AIQEHIDP R Sbjct: 121 TRRDMVESMSATAERIAHYEHVHGKAEVERFLDAVLAIQEHIDPSLVRPKLSWSMEDEPE 180 Query: 169 -----------------SGRPGGFREIGGEV------KDVIGYIAAESRYLEEWQREILY 205 + + G RE + KD++ +I SR LE WQR++L Sbjct: 181 EEIKPKRSEFDDLWNLDATKEPGRRESKKKAFPPKPEKDILLFIEEYSRELEPWQRDVLT 240 Query: 206 MLRAEMLYFWPQMETKIMNEGWASYWHLKLVRELDLSDSEIIEFSKMNSGVIQPSPGRIN 265 M+R EMLYFWPQ+ETKIMNEGWASYWH +++RE+DL+ +E +EF+K+N+ V+QPS IN Sbjct: 241 MMREEMLYFWPQLETKIMNEGWASYWHARIIREMDLTSAEAVEFAKLNANVVQPSRTTIN 300 Query: 266 PYYLGLMLLRHIE----------KREGLQA------LFEIREIESDVSFIRNYLTRELAE 309 PYYLGL + IE KR+G++ +FE+REIESD+SFIRNYLT++L Sbjct: 301 PYYLGLKIFEDIEQCYNEPSEEMKRQGVKPGSGREKIFEVREIESDLSFIRNYLTKDLVM 360 Query: 310 EMDLFVFKKE 319 D+++F+K+ Sbjct: 361 REDMYLFQKQ 370 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.139 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 497,775,179 Number of extensions: 21542920 Number of successful extensions: 43971 Number of sequences better than 1.0e-05: 63 Number of HSP's gapped: 43831 Number of HSP's successfully gapped: 99 Length of query: 323 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 197 Effective length of database: 415,135,104 Effective search space: 81781615488 Effective search space used: 81781615488 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 126 (53.1 bits)