BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0025.p (401 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO83851.1| vesicular monoamine transporter [Lymnaea stagnalis] 148 2e-34 ref|XP_313775.1| ENSANGP00000016901 [Anopheles gambiae] >gi|2129... 138 3e-31 ref|NP_725308.1| CG6119-PB [Drosophila melanogaster] >gi|2162724... 136 1e-30 ref|NP_610881.1| CG6119-PA [Drosophila melanogaster] >gi|7303303... 136 1e-30 gb|AAN71386.1| RE38567p [Drosophila melanogaster] 135 2e-30 >gb|AAO83851.1| vesicular monoamine transporter [Lymnaea stagnalis] Length = 532 Score = 148 bits (374), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 102/343 (29%), Positives = 166/343 (48%), Gaps = 6/343 (1%) Query: 26 IYGLVVPILPKYASALGASQTEIGLLFGSYAIALFIATPVFGALSDRIGRRGPMLWXXXX 85 +Y V P+ A L A E+G +F S AI IA P G +++RIG PM Sbjct: 130 VYMTVTEKSPRRAD-LDAENIEVGFMFASKAIMQLIANPFIGPITNRIGYTIPMFTGFFI 188 Query: 86 XXXXXXXXXXXEEYWMXXXXXXXXXXXXXITWTSGLAILADLYPSE-ERGKAMGLALSGQ 144 E Y + + +G+ ++A YP + ERG AMG+AL G Sbjct: 189 MMVSTIIFAFGETYAVLFAARTVQGIGSSCSSVAGMGMIASYYPDDKERGNAMGIALGGL 248 Query: 145 AAGILLGPSLGGWMYQLGGYKLPFLFAAVLAIADGVLRIFLLRD--LPERKSGTFLSSFG 202 A G+L+GP GG MY+ G + PFL A LA DG L++ ++ PE + GT Sbjct: 249 ALGVLVGPPFGGVMYEFAGKEAPFLILAGLAFLDGCLQLLAMQPSVKPESQEGTAFKE-- 306 Query: 203 LLRNRSLLLISGVVIIGSAIPSVLEPTLPLHLQEKFNAEPGFIGMLFAIPTLSYALATPW 262 LLR +L+ +G + G+ +++EP+LP+ + + ++ G+ F ++SYAL T Sbjct: 307 LLREPYILICAGAITFGNMGIAMMEPSLPIWMYKTMHSSEWQQGIAFLPASISYALGTNP 366 Query: 263 IGKLSSRIGYKKAVLIGLAVVAISLPLSALPDSMWMQAASLVLLGISMGMVLAPCLPALA 322 G L+ R+G + +IG+ +V+I S+ A +G ++GMV + +P + Sbjct: 367 FGPLAHRMGRWLSAMIGMIIVSICCFALPFAKSLTHLIAPNFGMGFAIGMVDSSMMPHMG 426 Query: 323 DISEQSGLPSYGITFAIYNTAYSFGMIVGPVTSGVLTDALGLK 365 + + + YG +AI + A+ G +GP SG + +G Sbjct: 427 YLVDLRHVSVYGSVYAIADVAFCLGFAIGPALSGTIVSRVGFH 469 >ref|XP_313775.1| ENSANGP00000016901 [Anopheles gambiae] gb|EAA09208.1| ENSANGP00000016901 [Anopheles gambiae str. PEST] Length = 494 Score = 138 bits (347), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 102/355 (28%), Positives = 172/355 (48%), Gaps = 9/355 (2%) Query: 47 EIGLLFGSYAIALFIATPVFGALSDRIGRRGPMLWXXXXXXXXXXXXXXXEEYWMXXXXX 106 E+GL+F S A +A P+ G L+ +IG PM Y + Sbjct: 143 EVGLMFASKAFVQLLANPIVGPLTHKIGYSIPMFAGFVIMFISTLIFAFGRTYSVLFLAR 202 Query: 107 XXXXXXXXITWTSGLAILADLYPSE-ERGKAMGLALSGQAAGILLGPSLGGWMYQLGGYK 165 + SG+ +LAD Y + ERG AMG+AL G A G+L+GP GG MY+ G Sbjct: 203 ALQGIGSSCSSVSGMGMLADRYTDDKERGNAMGIALGGLALGVLIGPPFGGIMYEFVGKS 262 Query: 166 LPFLFAAVLAIADGVLRIFLLRDLPERKSGTFLSSFGLLRNRSLLLISGVVIIGSAIPSV 225 PFL + LA+ DG+L++ +L+ + S L+ + +++ +G + + ++ Sbjct: 263 APFLVLSALALGDGLLQLIMLQPSVVIEESDPPSLKALVTDPYIIIAAGAITFANMGIAM 322 Query: 226 LEPTLPLHLQEKFNAEPGFIGMLFAIPTLSYALATPWIGKLSSRIGYKKAVLIGLAVVAI 285 LEP+LP+ + + A G+ F ++SY + T G L RIG A L+GL ++ + Sbjct: 323 LEPSLPIWMMDNMGASRWEQGVTFLPASISYLIGTNLFGPLGHRIGRWLAALLGLVIIGL 382 Query: 286 SL---PLSALPDSMWMQAASLVLLGISMGMVLAPCLPALADISEQSGLPSYGITFAIYNT 342 L P++ + + + A LG ++GMV + +P L + + YG +AI + Sbjct: 383 CLLCIPMATSINHLILPNAG---LGFAIGMVDSCMMPELGYLVDIRHSAVYGSVYAIGDV 439 Query: 343 AYSFGMIVGPVTSGVLTDALGLKLSYLSVGCAALLYMFIMLPSFRRTEVTRLNSK 397 A+ G +GP SG L + +G + ++ VG + L + + L +F R T+ K Sbjct: 440 AFCLGFAIGPALSGTLVNTIGFE--WMLVGISILCFAYAPLLTFLRAPPTKEEKK 492 >ref|NP_725308.1| CG6119-PB [Drosophila melanogaster] gb|AAM68587.1| CG6119-PB [Drosophila melanogaster] Length = 462 Score = 136 bits (342), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 106/373 (28%), Positives = 173/373 (46%), Gaps = 7/373 (1%) Query: 34 LPKYASALGASQTEIGLLFGSYAIALFIATPVFGALSDRIGRRGPMLWXXXXXXXXXXXX 93 L + + L E+GLLF S A + P+ G L+ RIG PM Sbjct: 77 LEERHNELVGETVEVGLLFASKAFVQLLVNPIVGPLTHRIGYSIPMFAGFVIMFLSTIIF 136 Query: 94 XXXEEYWMXXXXXXXXXXXXXITWTSGLAILADLYPSE-ERGKAMGLALSGQAAGILLGP 152 Y + + SG+ +LAD + + ERG AMG+AL G A G+L+GP Sbjct: 137 AFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRFTDDKERGNAMGIALGGLALGVLIGP 196 Query: 153 SLGGWMYQLGGYKLPFLFAAVLAIADGVLRIFLLRDLPERKSGTFLSSFGLLRNRSLLLI 212 GG MY+ G PFL A LA+ DG+L++F+L+ ++ S L+ + +L+ Sbjct: 197 PFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKAETEPPSLKSLISDPYILIA 256 Query: 213 SGVVIIGSAIPSVLEPTLPLHLQEKFNAEPGFIGMLFAIPTLSYALATPWIGKLSSRIGY 272 +G + + ++LEP+LPL + + A G+ F ++SY + T G L +IG Sbjct: 257 AGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIGR 316 Query: 273 KKAVLIGLAVVAISLPLSALPDSMWMQAASLVLLGISMGMVLAPCLPALADISEQSGLPS 332 A +GL ++ L + S+ LG ++GMV + +P L + + Sbjct: 317 WFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAV 376 Query: 333 YGITFAIYNTAYSFGMIVGPVTSGVLTDALGLKLSYLSVGCAALLYMFIMLPSFRRTEVT 392 YG +A+ + A+ G VGP SG L ++G + ++ G A L +M+ L + + T Sbjct: 377 YGSVYALGDVAFCVGFAVGPALSGSLVKSIGFE--WMLFGIAILCFMYAPLLTLLKNPPT 434 Query: 393 RLNSKA----QDR 401 K+ +DR Sbjct: 435 SDEKKSLIYGRDR 447 >ref|NP_610881.1| CG6119-PA [Drosophila melanogaster] gb|AAF58363.1| CG6119-PA [Drosophila melanogaster] Length = 498 Score = 136 bits (342), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 104/367 (28%), Positives = 170/367 (46%), Gaps = 3/367 (0%) Query: 34 LPKYASALGASQTEIGLLFGSYAIALFIATPVFGALSDRIGRRGPMLWXXXXXXXXXXXX 93 L + + L E+GLLF S A + P+ G L+ RIG PM Sbjct: 77 LEERHNELVGETVEVGLLFASKAFVQLLVNPIVGPLTHRIGYSIPMFAGFVIMFLSTIIF 136 Query: 94 XXXEEYWMXXXXXXXXXXXXXITWTSGLAILADLYPSE-ERGKAMGLALSGQAAGILLGP 152 Y + + SG+ +LAD + + ERG AMG+AL G A G+L+GP Sbjct: 137 AFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRFTDDKERGNAMGIALGGLALGVLIGP 196 Query: 153 SLGGWMYQLGGYKLPFLFAAVLAIADGVLRIFLLRDLPERKSGTFLSSFGLLRNRSLLLI 212 GG MY+ G PFL A LA+ DG+L++F+L+ ++ S L+ + +L+ Sbjct: 197 PFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKAETEPPSLKSLISDPYILIA 256 Query: 213 SGVVIIGSAIPSVLEPTLPLHLQEKFNAEPGFIGMLFAIPTLSYALATPWIGKLSSRIGY 272 +G + + ++LEP+LPL + + A G+ F ++SY + T G L +IG Sbjct: 257 AGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIGR 316 Query: 273 KKAVLIGLAVVAISLPLSALPDSMWMQAASLVLLGISMGMVLAPCLPALADISEQSGLPS 332 A +GL ++ L + S+ LG ++GMV + +P L + + Sbjct: 317 WFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAV 376 Query: 333 YGITFAIYNTAYSFGMIVGPVTSGVLTDALGLKLSYLSVGCAALLYMFIMLPSFRRTEVT 392 YG +A+ + A+ G VGP SG L ++G + ++ G A L +M+ L + + T Sbjct: 377 YGSVYALGDVAFCVGFAVGPALSGSLVKSIGFE--WMLFGIAILCFMYAPLLTLLKNPPT 434 Query: 393 RLNSKAQ 399 K + Sbjct: 435 SDEKKVR 441 >gb|AAN71386.1| RE38567p [Drosophila melanogaster] Length = 610 Score = 135 bits (339), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 107/373 (28%), Positives = 173/373 (46%), Gaps = 7/373 (1%) Query: 34 LPKYASALGASQTEIGLLFGSYAIALFIATPVFGALSDRIGRRGPMLWXXXXXXXXXXXX 93 L + + L E+GLLF S A + P+ G L+ RIG PM Sbjct: 225 LEERHNELVGETVEVGLLFASKAFVQLLVNPIVGPLTHRIGYSIPMFAGFVIMFLSTIIF 284 Query: 94 XXXEEYWMXXXXXXXXXXXXXITWTSGLAILADLYPSE-ERGKAMGLALSGQAAGILLGP 152 Y + + SG+ +LAD + + ERG AMG+AL G A G+L+GP Sbjct: 285 AFGRSYLVLFVARALQGIGSSCSSVSGMGMLADRFTDDKERGNAMGIALGGLALGVLIGP 344 Query: 153 SLGGWMYQLGGYKLPFLFAAVLAIADGVLRIFLLRDLPERKSGTFLSSFGLLRNRSLLLI 212 GG MY+ G PFL A LA+ DG+L++F+L+ ++ S L+ + +L+ Sbjct: 345 PFGGVMYEFVGKSAPFLILAALALGDGLLQLFMLQPSIQKAETEPPSLKSLISDPYILIA 404 Query: 213 SGVVIIGSAIPSVLEPTLPLHLQEKFNAEPGFIGMLFAIPTLSYALATPWIGKLSSRIGY 272 +G + + ++LEP+LPL + + A G+ F ++SY + T G L +IG Sbjct: 405 AGAITFANMGIAMLEPSLPLWMVDNMGATRWEQGVAFLPASISYLIGTNLFGPLGHKIGR 464 Query: 273 KKAVLIGLAVVAISLPLSALPDSMWMQAASLVLLGISMGMVLAPCLPALADISEQSGLPS 332 A +GL ++ L + S+ LG ++GMV + +P L + + Sbjct: 465 WFAACLGLIIIGGCLIFIPMATSITHLIIPNAGLGFAIGMVDSSMMPELGYLVDIRHSAV 524 Query: 333 YGITFAIYNTAYSFGMIVGPVTSGVLTDALGLKLSYLSVGCAALLYMFIMLPSFRRTEVT 392 YG +A+ + A+ G VGP SG L ++G + ++ G A L +M+ L + R T Sbjct: 525 YGSVYALGDVAFCVGFAVGPALSGSLVKSIGFE--WMLFGIAILCFMYAPLLTLLRNPPT 582 Query: 393 RLNSKA----QDR 401 K+ +DR Sbjct: 583 SDEKKSLIYGRDR 595 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.324 0.141 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 518,593,012 Number of extensions: 20754815 Number of successful extensions: 91895 Number of sequences better than 1.0e-05: 626 Number of HSP's gapped: 91753 Number of HSP's successfully gapped: 698 Length of query: 401 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 273 Effective length of database: 409,704,906 Effective search space: 111849439338 Effective search space used: 111849439338 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)