BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0007.p (296 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_815894.1| ABC transporter, permease protein [Enterococcus... 209 4e-53 ref|NP_242731.1| sugar transport system (permease) (binding prot... 199 7e-50 ref|NP_469563.1| similar to sugar ABC transporters, permease pro... 197 3e-49 ref|ZP_00017971.1| hypothetical protein [Chloroflexus aurantiacus] 187 2e-46 ref|NP_694345.1| sugar binding-protein dependent transporter sys... 186 6e-46 >ref|NP_815894.1| ABC transporter, permease protein [Enterococcus faecalis V583] gb|AAO81964.1| ABC transporter, permease protein [Enterococcus faecalis V583] Length = 300 Score = 209 bits (533), Expect = 4e-53, Method: Compositional matrix adjust. Identities = 115/271 (42%), Positives = 167/271 (61%), Gaps = 5/271 (1%) Query: 19 FIGPNMIGVILFFIIPAIYSFYLMFTDYK-FMSPDTKFVGLDNIQRMLGDEVFYIAIKNT 77 FI PN IG LF +IP I S L F + F +P+ FVG+ N +M+ D+ F+I++K T Sbjct: 24 FIAPNFIGFFLFTLIPVICSLILAFMSWDSFSTPE--FVGMKNFTKMIHDDTFWISLKQT 81 Query: 78 LMFLLSV-PVSIGLAFIVAVVLNRSVYLKKLLRALYFMPYITSGVAVAFVWMLLFQPNNG 136 ++ + V P+++ + +A++LNR + K R +F PY+TS VA+A VW +LF P G Sbjct: 82 FIYTIGVVPLTLICSLGLAILLNRKIRGMKFFRTAFFFPYVTSLVAIAVVWNMLFHPTMG 141 Query: 137 PINGILRAIGIANPPGWLSTMESSMYAIDIIWIWFMLGYNMIIYLAALQEVSGELLEAAK 196 PIN L+ + I NPPGW S+ ++ AI I+ +W +GY MI+YLA LQ V EL EAA Sbjct: 142 PINQFLK-LFIENPPGWTSSSTWALPAIIIVSVWRFMGYYMILYLAGLQSVPRELYEAAS 200 Query: 197 IDGARTWQTVRSILWPLVSPTTFLLLITGLIMSIKQFGIIQAITQGGPGNSTTVLSLFIY 256 +DGA W ++ P + PTTF + I +I K F ++Q +T GGPG +T VL IY Sbjct: 201 MDGAGKWNQFLNVTLPSLRPTTFFVTIMLVINCFKVFDLVQVMTGGGPGRATNVLVYEIY 260 Query: 257 QNAFRYYEMGYASAVSWALFVIIMIFTVVQW 287 AF + GYASA++ LF+I++ TV Q+ Sbjct: 261 NEAFVKFNFGYASAIAMVLFIIVLAITVAQF 291 >ref|NP_242731.1| sugar transport system (permease) (binding protein dependent transporter) [Bacillus halodurans] pir||A83883 sugar transport system (permease) (binding protein dependent transporter) BH1865 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05584.1| sugar transport system (permease) (binding protein dependent transporter) [Bacillus halodurans] Length = 309 Score = 199 bits (505), Expect = 7e-50, Method: Compositional matrix adjust. Identities = 120/303 (39%), Positives = 183/303 (60%), Gaps = 10/303 (3%) Query: 2 NDVKTSWMKRQQHL-------GYLFIGPNMIGVILFFIIPAIYSFYLMFTDYKFMSPDTK 54 + KT+ ++Q+ GYLFI P +IG +F + P +YS Y+ FTD+ + + Sbjct: 9 RETKTTKARKQKRRLNKEYFWGYLFIAPPIIGFAIFALGPMLYSIYVSFTDFDLYN-EPV 67 Query: 55 FVGLDNIQRM-LGDEVFYIAIKNTLMFLLSVPVSIGLAFIVAVVLNRSVYLKKLLRALYF 113 + G DN R+ + D++F + NT L +P+ + ++ +AV LN+ V L R +F Sbjct: 68 WTGADNYYRLFVTDDLFRKTVFNTFYAALGIPIGMAVSLGIAVALNQKVKGIALFRTAFF 127 Query: 114 MPYITSGVAVAFVWMLLFQPNNGPINGILRAIGIANPPGWLSTMESSMYAIDIIWIWFML 173 +P ++S VA+ +W +F + G +N +L +GI + PGWLS + +M A+ I +W L Sbjct: 128 LPAVSSVVAITLLWRWIFNADFGLLNIMLNYVGI-HGPGWLSDEKWAMPAMIIQGVWGGL 186 Query: 174 GYNMIIYLAALQEVSGELLEAAKIDGARTWQTVRSILWPLVSPTTFLLLITGLIMSIKQF 233 G NMI+YLAALQ V+ L EAA IDG WQ I P +SPTTF +LIT I +++ F Sbjct: 187 GINMILYLAALQGVNPALYEAADIDGGNAWQKFIHITVPSISPTTFFILITSTIGALQDF 246 Query: 234 GIIQAITQGGPGNSTTVLSLFIYQNAFRYYEMGYASAVSWALFVIIMIFTVVQWLGQKRW 293 +T+GGP STT + +++ NAFRY EMGYASA++W L +II+I T++ + K+W Sbjct: 247 QRFMIMTEGGPNYSTTTVVYYLFLNAFRYMEMGYASAMAWVLGIIILIITIINFKLAKKW 306 Query: 294 VHY 296 VHY Sbjct: 307 VHY 309 >ref|NP_469563.1| similar to sugar ABC transporters, permease proteins [Listeria innocua] ref|NP_463710.1| similar to sugar ABC transporters, permease proteins [Listeria monocytogenes EGD-e] pir||AC1460 sugar ABC transporters, permease protein homolog lin0218 [imported] - Listeria innocua (strain Clip11262) pir||AD1097 sugar ABC transporters, permease proteins homolog lmo0179 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC98394.1| lmo0179 [Listeria monocytogenes] emb|CAC95451.1| lin0218 [Listeria innocua] Length = 292 Score = 197 bits (501), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 119/291 (40%), Positives = 181/291 (62%), Gaps = 5/291 (1%) Query: 9 MKRQQHLGYL--FIGPNMIGVILFFIIPAIYSFYLMFTDYKFMSPDTKFVGLDNIQRMLG 66 M R++ L +L F+ PN++G ++F IP + S + FTD+ +S F G +N +R+ Sbjct: 4 MTRKKTLFWLCIFLLPNLLGFLIFIAIPILGSLGISFTDWDLVS-TIHFTGFENYKRLFN 62 Query: 67 DEVFYIAIKNTLMFLLS-VPVSIGLAFIVAVVLNRSVYLKKLLRALYFMPYITSGVAVAF 125 D F+ + +NT+ F++ +P + L A++LN+ + L+ RA+YF+P ITS VAV+ Sbjct: 63 DPEFWKSFRNTITFIIGYLPSVMILGLACALMLNQKIKLRPFFRAVYFLPVITSWVAVSL 122 Query: 126 VWMLLFQPNNGPINGILRAIGIANPPGWLSTMESSMYAIDIIWIWFMLGYNMIIYLAALQ 185 VW LF P+ G IN L IGIA P WL+ +++M A+ I W +G+ M+++L LQ Sbjct: 123 VWKWLFNPSYGLINYFLSLIGIAGP-NWLTDPQTAMIAVIITSAWKDIGFVMVLFLGGLQ 181 Query: 186 EVSGELLEAAKIDGARTWQTVRSILWPLVSPTTFLLLITGLIMSIKQFGIIQAITQGGPG 245 +S EAA +DGA + + SI PL++PTTF + I LI S + F + +T+GGP Sbjct: 182 NISPSYYEAANMDGASKLRQLFSITIPLLTPTTFFVTIISLINSFQVFDQVMIMTEGGPS 241 Query: 246 NSTTVLSLFIYQNAFRYYEMGYASAVSWALFVIIMIFTVVQWLGQKRWVHY 296 +T L IY +AFRY+EMGYASA+SW LF++I I T++Q QKRW +Y Sbjct: 242 GATMTLVQNIYNHAFRYFEMGYASAMSWILFIVIFIITIIQNKLQKRWSNY 292 >ref|ZP_00017971.1| hypothetical protein [Chloroflexus aurantiacus] Length = 313 Score = 187 bits (476), Expect = 2e-46, Method: Compositional matrix adjust. Identities = 108/281 (38%), Positives = 168/281 (59%), Gaps = 8/281 (2%) Query: 22 PNMIGVILFFIIPAIYSFYLMFTDYKFMSPDTKFVGLDNIQRMLGDEVFYIAIKNTLMF- 80 P ++GV++FF++P SFYL FTD + D +G N QR L D FY A+ NT F Sbjct: 34 PTILGVLIFFLVPLGISFYLSFTDARLFG-DPNIIGFSNYQRALSDPTFYRAMWNTAAFS 92 Query: 81 LLSVPVSIGLAFIVAVVLNRSVYLKKLLRALYFMPYITSGVAVAFVWMLLFQPNNGPING 140 ++++ VS A ++AV+LN + + RA++F+P + S V V +W L + G +N Sbjct: 93 MVTLVVSTVPALLLAVILNERIAGRTFFRAVFFIPVVASVVGVTLLWRYLLNIDFGFVNY 152 Query: 141 ILRAIGIANPPGWLSTMESSMYAIDIIWIWFMLGYNMIIYLAALQEVSGELLEAAKIDGA 200 LR G+ P WL++ E + ++ +++ W +GYNM+I+LA LQ V +L EAA +DGA Sbjct: 153 ALRLFGL-QPIPWLTSPEWGLISVILVFSWKTIGYNMVIFLAGLQGVPPQLYEAASLDGA 211 Query: 201 RTWQTVRSILWPLVSPTTFLLLITGLIMSIKQFGIIQAI-----TQGGPGNSTTVLSLFI 255 WQ I P++SPTTF +L+T LI ++ F + A+ GP +S + + Sbjct: 212 SRWQQFLFITVPMLSPTTFFVLVTTLINCLQVFDVPVALGLTRSNTVGPADSMLTVVPLL 271 Query: 256 YQNAFRYYEMGYASAVSWALFVIIMIFTVVQWLGQKRWVHY 296 ++ AF MGYASA++W LFVII++ T++Q+ +RWVHY Sbjct: 272 WREAFIGGRMGYASALAWLLFVIILLLTLIQFRISRRWVHY 312 >ref|NP_694345.1| sugar binding-protein dependent transporter system permease [Oceanobacillus iheyensis HTE831] dbj|BAC15379.1| sugar binding-protein dependent transporter system permease [Oceanobacillus iheyensis HTE831] Length = 323 Score = 186 bits (472), Expect = 6e-46, Method: Compositional matrix adjust. Identities = 115/295 (38%), Positives = 179/295 (60%), Gaps = 17/295 (5%) Query: 11 RQQHLGYLFIGPNMIGVILFFIIPAIYSFYLMFTDYKFMSPDTKFVGLDNIQRMLGDEVF 70 ++ + YLFI P ++G+ +F+I+PA+ SF L FT++ ++ +FV LDN M+ D +F Sbjct: 35 KEAMVAYLFIAPTLLGLFIFYILPALGSFVLSFTNWDGIN-TPEFVALDNFILMIQDPLF 93 Query: 71 YIAIKNTLMF-LLSVPVSIGLAFIVAVVLNRSVYLKKLLRALYFMPYITSGVAVAFVWML 129 +I NT +F LLSVP+SI A I++++LN+ + R LYF+P +T VAV VW Sbjct: 94 MRSIINTAVFTLLSVPLSIMFAIIISLLLNQKIKGMVFYRTLYFLPVVTMPVAVGMVWKW 153 Query: 130 LFQPNNGPINGILRAIGIAN--PPGWLSTMESSMYAIDIIWIWFMLGYNMIIYLAALQEV 187 L+ G +N IL GI N P WL S+ ++ I+++W +G N+I+ LA LQ + Sbjct: 154 LYNTEYGIVNHIL---GIFNLPQPAWLFDPNISLISVIIVYVWMTVGNNVILVLAGLQGI 210 Query: 188 SGELLEAAKIDGARTWQTVRSILWPLVSPTTFLLLITGLIMSIKQFGIIQAITQGGPGNS 247 EAA+IDGA + I PL++PT F +LITG+I S++ F +I + GN+ Sbjct: 211 EKSYYEAAQIDGASKTKQFFKITLPLLTPTLFFVLITGMISSLQVFDLIFVMI----GNT 266 Query: 248 TTVLSLF------IYQNAFRYYEMGYASAVSWALFVIIMIFTVVQWLGQKRWVHY 296 T +L +Y+ F+Y +MG ASA + LF+ I++ T+VQ++ QK+WVHY Sbjct: 267 TALLEPLRTLVYGVYEFGFKYGQMGVASAQALILFLTILLVTIVQFIFQKKWVHY 321 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.330 0.143 0.450 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 445,253,427 Number of extensions: 17512564 Number of successful extensions: 71125 Number of sequences better than 1.0e-05: 1453 Number of HSP's gapped: 69432 Number of HSP's successfully gapped: 1455 Length of query: 296 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 171 Effective length of database: 417,850,203 Effective search space: 71452384713 Effective search space used: 71452384713 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 125 (52.8 bits)