BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0003.p (1416 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|Q06852|SLP1_CLOTM CELL SURFACE GLYCOPROTEIN 1 PRECURSOR (OUTE... 182 5e-44 ref|ZP_00061707.1| COG0515: Serine/threonine protein kinase [Clo... 179 4e-43 ref|ZP_00061708.1| COG1463: ABC-type transport system involved i... 177 1e-42 ref|ZP_00097676.1| COG2931: RTX toxins and related Ca2+-binding ... 174 2e-41 ref|ZP_00061706.1| hypothetical protein [Clostridium thermocellu... 169 4e-40 >sp|Q06852|SLP1_CLOTM CELL SURFACE GLYCOPROTEIN 1 PRECURSOR (OUTER LAYER PROTEIN B) (S-LAYER PROTEIN 1) pir||T18262 S-layer protein - Clostridium thermocellum emb|CAA47841.1| S-layer protein [Clostridium thermocellum] Length = 1664 Score = 182 bits (462), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 90/211 (42%), Positives = 128/211 (60%), Gaps = 13/211 (6%) Query: 1215 HGAYIVGFPDGEFKPDEQVTRAQMALMIARNLGYDAN-AAVGSLPFGDVAVRHYAARAIA 1273 H AY+ G+PDG F+P+ +TRA+ A++ A+ LG D + A + P+ D+A H+AA AI Sbjct: 1451 HRAYLRGYPDGSFRPERNITRAEAAVIFAKLLGADESYGAQSASPYSDLADTHWAAWAIK 1510 Query: 1274 FVNAQGIMKGDPNGQFRAAAPITRAEMAAVAANYM----------KLAVSADGKSSFNDT 1323 F +QG+ KG P+G F+ ITRAE A V +++ KLA F+D Sbjct: 1511 FATSQGLFKGYPDGTFKPDQNITRAEFATVVLHFLTKVKGQEIMSKLATIDISNPKFDDC 1570 Query: 1324 AGHWAQSVIEANLTAGLFKGYPDGSFKPNAYLTRAEAVVMVNQMFDRGPLYGADATKFPD 1383 GHWAQ IE + G GYPDG+FKP Y+ R+E+V ++N+ +RGPL GA FPD Sbjct: 1571 VGHWAQEFIEKLTSLGYISGYPDGTFKPQNYIKRSESVALINRALERGPLNGA-PKLFPD 1629 Query: 1384 VSGSHWAYRDIQEAVIDHKYRIDGEQKEQLV 1414 V+ S+WA+ DI + +DH Y I+ E KE+ V Sbjct: 1630 VNESYWAFGDIMDGALDHSYIIEDE-KEKFV 1659 >ref|ZP_00061707.1| COG0515: Serine/threonine protein kinase [Clostridium thermocellum ATCC 27405] sp|Q06853|SLP2_CLOTM CELL SURFACE GLYCOPROTEIN 2 PRECURSOR (S-LAYER PROTEIN 2) pir||T18263 S-layer protein - Clostridium thermocellum emb|CAA47842.1| S-layer protein [Clostridium thermocellum] Length = 688 Score = 179 bits (454), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 88/208 (42%), Positives = 129/208 (62%), Gaps = 12/208 (5%) Query: 1215 HGAYIVGFPDGEFKPDEQVTRAQMALMIARNLGYDANAAVG-SLPFGDVAVRHYAARAIA 1273 H +Y+ G+PD F+P++ +TRA+ A++ A+ LG + N + ++ + DV H+A+ AI Sbjct: 480 HYSYLTGYPDKMFRPEKSITRAEAAVIFAKLLGANENTKINYNVSYTDVDSSHWASWAIK 539 Query: 1274 FVNAQGIMKGDPNGQFRAAAPITRAEMAAVAANYMKLAVSADG-------KSSFNDTAGH 1326 FV+ + + G P+G F+ ITRAE + V KL VS G KS F DT GH Sbjct: 540 FVSYKKLFTGYPDGSFKPNQNITRAEFSTVV---FKLLVSEKGLKEEKIEKSKFGDTKGH 596 Query: 1327 WAQSVIEANLTAGLFKGYPDGSFKPNAYLTRAEAVVMVNQMFDRGPLYGADATKFPDVSG 1386 WAQ IE G GYPDG+FKPN + R+E+V ++N+ RGPL+GA F DV Sbjct: 597 WAQQFIEQLSDLGYINGYPDGTFKPNNNIKRSESVALINRAMGRGPLHGAPQV-FEDVPQ 655 Query: 1387 SHWAYRDIQEAVIDHKYRIDGEQKEQLV 1414 +HWA++DI E V++H+Y++D E KEQL+ Sbjct: 656 THWAFKDIAEGVLNHRYKLDNEGKEQLL 683 >ref|ZP_00061708.1| COG1463: ABC-type transport system involved in resistance to organic solvents, periplasmic component [Clostridium thermocellum ATCC 27405] Length = 723 Score = 177 bits (450), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 90/211 (42%), Positives = 128/211 (60%), Gaps = 13/211 (6%) Query: 1215 HGAYIVGFPDGEFKPDEQVTRAQMALMIARNLGYDAN-AAVGSLPFGDVAVRHYAARAIA 1273 H AY+ G+PDG F+P+ +TRA+ A++ A+ LG D + A + P+ D+A H+AA AI Sbjct: 510 HRAYLRGYPDGSFRPERNITRAEAAVIFAKLLGADESYGAQSASPYSDLADTHWAAWAIK 569 Query: 1274 FVNAQGIMKGDPNGQFRAAAPITRAEMAAVAANYM----------KLAVSADGKSSFNDT 1323 F +QG+ KG P+G F+ ITRAE A V +++ KLA F+D Sbjct: 570 FATSQGLFKGYPDGTFKPDQNITRAEFATVVLHFLTKVKGQEIMSKLATIDISNPKFDDC 629 Query: 1324 AGHWAQSVIEANLTAGLFKGYPDGSFKPNAYLTRAEAVVMVNQMFDRGPLYGADATKFPD 1383 GHWAQ IE + G GYPDG+FKP Y+ R+E+V ++N+ +RGPL GA FPD Sbjct: 630 VGHWAQEFIEKLTSLGYISGYPDGTFKPQNYIKRSESVALINRALERGPLNGA-PKLFPD 688 Query: 1384 VSGSHWAYRDIQEAVIDHKYRIDGEQKEQLV 1414 V+ S+WA+ DI + +DH Y I+ E KE+ V Sbjct: 689 VNESYWAFGDIMDGALDHSYIIEDE-KEKFV 718 Score = 60.1 bits (144), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 27/62 (43%), Positives = 41/62 (66%), Gaps = 1/62 (1%) Query: 1215 HGAYIVGFPDGEFKPDEQVTRAQMALMIARNLGYDAN-AAVGSLPFGDVAVRHYAARAIA 1273 H AY+ G+PDG F+P+ +TRA+ A++ A+ LG D + A + P+ D+A H+AA AI Sbjct: 174 HRAYLRGYPDGSFRPERNITRAEAAVIFAKLLGADESYGAQSASPYSDLADTHWAAWAIK 233 Query: 1274 FV 1275 F Sbjct: 234 FA 235 >ref|ZP_00097676.1| COG2931: RTX toxins and related Ca2+-binding proteins [Desulfitobacterium hafniense] Length = 1549 Score = 174 bits (441), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 99/268 (36%), Positives = 148/268 (55%), Gaps = 6/268 (2%) Query: 952 RVSQEPSSGGGYVPAPDKTKTEVIEVDVXXXX-XXXXXXXKVPVQRTTRNDGTITDLVRF 1010 R S + SSGG P T + V ++ V ++ V+R T +DG TD V + Sbjct: 975 RSSDDESSGGNTTPV-SSTPSGVTQITVDVKQGNTDSTVAQITVERRTGSDGKKTDTVTY 1033 Query: 1011 TXXXXXXXXXXXXXTGQSIARVVIPDAADKVGEVNVQIPVETAKLLKENGIVLEMYTENA 1070 + AR+VIPD D+V E V IPV+ ++L + GI L++ TE A Sbjct: 1034 QESKAAETVKKLKEENKDTARIVIPDEKDEVAETKVNIPVQALEVLAQGGINLQIDTEEA 1093 Query: 1071 IIRIPNASLDGIAQ----DFYFRLVPVKSATERNEIEKRATLEAVVREVAGNNKIEVVAR 1126 I I ++ GI+Q D YFRLVPVK ++ +A L+A + ++ + +V Sbjct: 1094 KIDISKNTITGISQEVEGDLYFRLVPVKDEAQKKSTIDQALLKAALVSGNTDSSLSIVGN 1153 Query: 1127 PMTIETNLSSRAVTLILPLKDVKLPANEAERNAFLAQLGIFIEHTDGQKEVVKGKAVTYK 1186 P+TIETN+SS + LPL + LP+N AE+ A L QL ++IEH+DG KE+V+G+ V YK Sbjct: 1154 PVTIETNMSSTEADITLPLTGMTLPSNPAEKAALLQQLAVYIEHSDGDKELVQGELVEYK 1213 Query: 1187 EGLLGLEFSVTKFSTFTIINFNNQAAAS 1214 EG+ G+ F + KFS FT++ + A+S Sbjct: 1214 EGVYGIRFHINKFSIFTVVRTDAFLASS 1241 >ref|ZP_00061706.1| hypothetical protein [Clostridium thermocellum ATCC 27405] sp|Q06848|ANCA_CLOTM Cellulosome anchoring protein precursor pir||T18264 cellulosome anchoring protein - Clostridium thermocellum emb|CAA47843.1| cellulosome anchoring protein [Clostridium thermocellum] Length = 447 Score = 169 bits (429), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 86/208 (41%), Positives = 123/208 (59%), Gaps = 7/208 (3%) Query: 1215 HGAYIVGFPDGEFKPDEQVTRAQMALMIARNLGYDANAA--VGSLPFGDVAVRHYAARAI 1272 H ++ G+P G FKP+ +TRA+ A++ A+ LG D N+A S+ F D+ H+AA AI Sbjct: 239 HAPFLKGYPGGLFKPENNITRAEAAVIFAKLLGADENSAGKNSSITFKDLKDSHWAAWAI 298 Query: 1273 AFVNAQGIMKGDPNGQFRAAAPITRAEMAAVAANYM----KLAVSADGKSSFNDTAGHWA 1328 +V Q + G P+G F ITRAE A V ++ K+ D K+ D GHWA Sbjct: 299 KYVTEQNLFGGYPDGTFMPDKSITRAEFATVTYKFLEKLGKIEQGTDVKTQLKDIEGHWA 358 Query: 1329 QSVIEANLTAGLFKGYPDGSFKPNAYLTRAEAVVMVNQMFDRGPLYGADATKFPDVSGSH 1388 Q IE + G KGYPD +F+P A + RAE+V ++N+ +RGPL GA +F DV ++ Sbjct: 359 QKYIETLVAKGYIKGYPDETFRPQASIKRAESVALINRSLERGPLNGA-VLEFTDVPVNY 417 Query: 1389 WAYRDIQEAVIDHKYRIDGEQKEQLVSE 1416 WAY+DI E VI H Y+ID +E +V + Sbjct: 418 WAYKDIAEGVIYHSYKIDENGQEVMVEK 445 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.133 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 2,083,415,704 Number of extensions: 92100855 Number of successful extensions: 161931 Number of sequences better than 1.0e-05: 139 Number of HSP's gapped: 161581 Number of HSP's successfully gapped: 218 Length of query: 1416 Length of database: 757,237,578 Length adjustment: 138 Effective length of query: 1278 Effective length of database: 382,553,916 Effective search space: 488903904648 Effective search space used: 488903904648 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)