BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0249.0001.p (98 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00100726.1| COG1595: DNA-directed RNA polymerase speciali... 75 4e-13 ref|ZP_00100114.1| COG1595: DNA-directed RNA polymerase speciali... 58 8e-08 ref|ZP_00098468.1| COG1595: DNA-directed RNA polymerase speciali... 55 7e-07 >ref|ZP_00100726.1| COG1595: DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog [Desulfitobacterium hafniense] Length = 75 Score = 75.1 bits (183), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 32/65 (49%), Positives = 49/65 (75%) Query: 29 KLIDQAEALNVHKVLHALNEPFKEVFMLRVFGELSFDHISQIFGRTESWARVTYHRARLK 88 +++++ A +H++LH ++EP+KEVF LRVFGEL F+ I IF + SWARVT++RA+ K Sbjct: 4 QIMNEDTAFVIHQILHDMDEPYKEVFSLRVFGELPFEKIGLIFNKNASWARVTFYRAKNK 63 Query: 89 IQKLL 93 + K L Sbjct: 64 LLKTL 68 >ref|ZP_00100114.1| COG1595: DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog [Desulfitobacterium hafniense] Length = 174 Score = 57.8 bits (138), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 22/67 (32%), Positives = 47/67 (70%) Query: 28 QKLIDQAEALNVHKVLHALNEPFKEVFMLRVFGELSFDHISQIFGRTESWARVTYHRARL 87 ++L+ + ++ + + ++ L E ++ V +LR F ELS+ I++I G+TE+W RVT+ RA+ Sbjct: 98 EQLLQKEQSQTMQRAIYCLPETYRTVIILREFEELSYGEIAKILGKTENWVRVTFFRAKQ 157 Query: 88 KIQKLLM 94 K+++ ++ Sbjct: 158 KLKETIL 164 >ref|ZP_00098468.1| COG1595: DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog [Desulfitobacterium hafniense] Length = 166 Score = 54.7 bits (130), Expect = 7e-07, Method: Compositional matrix adjust. Identities = 26/57 (45%), Positives = 38/57 (66%) Query: 41 KVLHALNEPFKEVFMLRVFGELSFDHISQIFGRTESWARVTYHRARLKIQKLLMEDE 97 KVL L E ++EVF LR + EL+++ I+ I G++ W +VT HRARL+ +K E E Sbjct: 107 KVLQELPENYREVFWLREWHELAYEQIAVIIGKSVPWVKVTLHRARLQFRKNYAEKE 163 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 135,294,257 Number of extensions: 4380757 Number of successful extensions: 12910 Number of sequences better than 1.0e-05: 3 Number of HSP's gapped: 12911 Number of HSP's successfully gapped: 3 Length of query: 98 Length of database: 757,237,578 Length adjustment: 66 Effective length of query: 32 Effective length of database: 578,041,044 Effective search space: 18497313408 Effective search space used: 18497313408 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 120 (50.8 bits)