BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0034.p (417 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_242422.1| GTP cyclohydrolase II / 3, 4-dihydroxy-2-butano... 618 e-176 ref|NP_833829.1| GTP cyclohydrolase II [Bacillus cereus ATCC 145... 600 e-170 ref|NP_658151.1| DHBP_synthase, 3,4-dihydroxy-2-butanone 4-phosp... 593 e-168 ref|NP_980474.1| 3,4-dihydroxy-2-butanone 4-phosphate synthase/G... 592 e-168 ref|NP_390207.1| GTP cyclohydrolase II and 3,4-dihydroxy-2-butan... 588 e-166 >ref|NP_242422.1| GTP cyclohydrolase II / 3, 4-dihydroxy-2-butanone 4-phosphate synthase [Bacillus halodurans] pir||D83844 GTP cyclohydrolase II / 3, 4-dihydroxy-2-butanone 4-phosphate synthase ribA [imported] - Bacillus halodurans (strain C-125) dbj|BAB05275.1| GTP cyclohydrolase II / 3, 4-dihydroxy-2-butanone 4-phosphate synthase [Bacillus halodurans] Length = 404 Score = 618 bits (1594), Expect = e-176, Method: Compositional matrix adjust. Identities = 293/400 (73%), Positives = 342/400 (85%) Query: 7 NDSVLDPIEDAIYDLMRGKVIIVVDDEDRENEGDFIALAERSTPEVINFMITEGRGLVCV 66 + + DPIE+AIY+LM+G+V+IV DDEDRENEGDF+ALAE++TPEVINFMIT GRGLVC Sbjct: 2 DKKLFDPIEEAIYELMQGRVVIVCDDEDRENEGDFVALAEKATPEVINFMITHGRGLVCT 61 Query: 67 PITAERAAELDLQPMVAHNTDNHGTAFTVSVDHKDTTTGISAAERSKTIQALMDPEAKPS 126 PIT ERA ELDL PMV HNTD HGTAFTVS+DH+ TTTGISA ER+ TIQAL+D + K Sbjct: 62 PITEERAKELDLVPMVDHNTDPHGTAFTVSIDHQMTTTGISAHERAMTIQALIDKKTKKH 121 Query: 127 DFRRPGHMFPLIAKKGGVLRRSGHTEAAVDLARMCGAYPASVICEVVKEDGTMARLPDLV 186 F+RPGH+FPLIAK GGVLRR+GHTEAAVDLAR+ GA PA VICE++KEDG+MAR+PDL Sbjct: 122 HFKRPGHIFPLIAKNGGVLRRAGHTEAAVDLARLSGAEPAGVICEIIKEDGSMARVPDLR 181 Query: 187 EIARKHELKLISIKDLIHYRNEKEQLVTREVSVKLPTDFGDFQTIAYTNEVDDKEHVALV 246 +IA + ELK+I+IKDLI YR+ K++LV REV + LPTDFG F+ I YT+ +D KE VALV Sbjct: 182 KIADQFELKMITIKDLIEYRHRKDKLVKREVDISLPTDFGSFRAIGYTDVIDGKESVALV 241 Query: 247 KGDISGEEPVLVRVHSECLTGDVFHSHRCDCGPQFEAALRQIEAAGRGVLLYMRQEGRGI 306 KG I EP LVRVHSECLTGDVF SHRCDCGPQ +AAL QIE G+G+LLYMRQEGRGI Sbjct: 242 KGQIVEGEPTLVRVHSECLTGDVFGSHRCDCGPQLQAALTQIEQQGKGILLYMRQEGRGI 301 Query: 307 GLINKLRAYKLQEEGLDTVDANLKLGFPADLRDYGIGAQILKDLGVRQIKLMTNNPRKIK 366 GL+NKL+AYKLQEEG DTV+AN KLGFPADLRDYG+GAQIL+DLGV +++L+TNNPRKI Sbjct: 302 GLMNKLKAYKLQEEGYDTVEANEKLGFPADLRDYGMGAQILRDLGVSKMRLLTNNPRKIT 361 Query: 367 GLEGYGLEVVERVPIQMPENKDNTNYLHTKQAKLGHLLTF 406 GL+GYGLEVVERVP+Q+P NKDN YL TK KLGHLL F Sbjct: 362 GLKGYGLEVVERVPLQLPHNKDNERYLKTKHEKLGHLLNF 401 >ref|NP_833829.1| GTP cyclohydrolase II [Bacillus cereus ATCC 14579] gb|AAP11030.1| GTP cyclohydrolase II [Bacillus cereus ATCC 14579] Length = 397 Score = 600 bits (1547), Expect = e-170, Method: Compositional matrix adjust. Identities = 284/393 (72%), Positives = 331/393 (84%) Query: 14 IEDAIYDLMRGKVIIVVDDEDRENEGDFIALAERSTPEVINFMITEGRGLVCVPITAERA 73 IE+A+ DL +GKV+IV DDE+RENEGDFIALAE TPE INFMIT GRGLVCVPIT A Sbjct: 5 IEEALEDLKKGKVVIVCDDENRENEGDFIALAEYITPETINFMITHGRGLVCVPITEGYA 64 Query: 74 AELDLQPMVAHNTDNHGTAFTVSVDHKDTTTGISAAERSKTIQALMDPEAKPSDFRRPGH 133 L L+PMV+HNTD+H TAFTVS+DH TTTGISA ER+ TIQ L++P +K +DF RPGH Sbjct: 65 ERLQLEPMVSHNTDSHHTAFTVSIDHVSTTTGISAHERATTIQELLNPASKGADFNRPGH 124 Query: 134 MFPLIAKKGGVLRRSGHTEAAVDLARMCGAYPASVICEVVKEDGTMARLPDLVEIARKHE 193 +FPLIAK+GGVLRR+GHTEAAVDLA++CGA PA VICE++ EDGTMAR+PDL+E A++ + Sbjct: 125 IFPLIAKEGGVLRRAGHTEAAVDLAKLCGAEPAGVICEIINEDGTMARVPDLIECAKQFD 184 Query: 194 LKLISIKDLIHYRNEKEQLVTREVSVKLPTDFGDFQTIAYTNEVDDKEHVALVKGDISGE 253 +K+I+I+DLI YR E LVTRE + LPTDFG F I Y+N +D KEH+ALVKGDIS Sbjct: 185 IKMITIEDLIAYRRHHETLVTREAEITLPTDFGTFHAIGYSNSLDTKEHIALVKGDISTG 244 Query: 254 EPVLVRVHSECLTGDVFHSHRCDCGPQFEAALRQIEAAGRGVLLYMRQEGRGIGLINKLR 313 EPVLVRVHSECLTGDVF SHRCDCGPQ AAL QIE G+GVLLYMRQEGRGIGL+NKLR Sbjct: 245 EPVLVRVHSECLTGDVFGSHRCDCGPQLHAALAQIEREGKGVLLYMRQEGRGIGLLNKLR 304 Query: 314 AYKLQEEGLDTVDANLKLGFPADLRDYGIGAQILKDLGVRQIKLMTNNPRKIKGLEGYGL 373 AYKLQEEG DTV+AN KLGFPADLRDYGIGAQILKDLG++ ++L+TNNPRKI GL+GY L Sbjct: 305 AYKLQEEGFDTVEANEKLGFPADLRDYGIGAQILKDLGLQSLRLLTNNPRKIAGLQGYDL 364 Query: 374 EVVERVPIQMPENKDNTNYLHTKQAKLGHLLTF 406 EVVERVP+QMP ++N +YL TK KLGHLL Sbjct: 365 EVVERVPLQMPAKEENKSYLQTKVNKLGHLLNL 397 >ref|NP_658151.1| DHBP_synthase, 3,4-dihydroxy-2-butanone 4-phosphate synthase [Bacillus anthracis A2012] ref|NP_846566.1| 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II [Bacillus anthracis str. Ames] gb|AAP28052.1| 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II [Bacillus anthracis str. Ames] Length = 397 Score = 593 bits (1530), Expect = e-168, Method: Compositional matrix adjust. Identities = 282/393 (71%), Positives = 329/393 (83%) Query: 14 IEDAIYDLMRGKVIIVVDDEDRENEGDFIALAERSTPEVINFMITEGRGLVCVPITAERA 73 IE+A+ DL +GKV+IV DDE+RENEGDFIALAE TPE INFMIT GRGLVCVPIT A Sbjct: 5 IEEALEDLKQGKVVIVCDDENRENEGDFIALAEYITPETINFMITHGRGLVCVPITEGYA 64 Query: 74 AELDLQPMVAHNTDNHGTAFTVSVDHKDTTTGISAAERSKTIQALMDPEAKPSDFRRPGH 133 L L+PMV+HNTD+H TAFTVS+DH TTTGISA ER+ TIQ L++P +K +DF RPGH Sbjct: 65 ERLQLEPMVSHNTDSHHTAFTVSIDHVSTTTGISAHERATTIQQLLNPASKGADFNRPGH 124 Query: 134 MFPLIAKKGGVLRRSGHTEAAVDLARMCGAYPASVICEVVKEDGTMARLPDLVEIARKHE 193 +FPLIAK+GGVLRR+GHTEAAVDLA++CGA PA VICE++ EDGTMAR+PDL++ A++ + Sbjct: 125 IFPLIAKEGGVLRRAGHTEAAVDLAQLCGAEPAGVICEIINEDGTMARVPDLLQCAKQFD 184 Query: 194 LKLISIKDLIHYRNEKEQLVTREVSVKLPTDFGDFQTIAYTNEVDDKEHVALVKGDISGE 253 +K+I+I+DLI YR E LVTREV + LPTDFG FQ I Y+N +D KEH+ALVKGDIS Sbjct: 185 IKMITIEDLIAYRRHHETLVTREVEITLPTDFGTFQAIGYSNSLDTKEHIALVKGDISTG 244 Query: 254 EPVLVRVHSECLTGDVFHSHRCDCGPQFEAALRQIEAAGRGVLLYMRQEGRGIGLINKLR 313 EPVLVRVHSECLTGDVF S RCDCGPQ AAL QIE G+GVLLYMRQEGRGIGL+NKLR Sbjct: 245 EPVLVRVHSECLTGDVFGSCRCDCGPQLHAALAQIEREGKGVLLYMRQEGRGIGLLNKLR 304 Query: 314 AYKLQEEGLDTVDANLKLGFPADLRDYGIGAQILKDLGVRQIKLMTNNPRKIKGLEGYGL 373 AYKLQEEG DTV+AN KLGFPADLRDYGIGAQILKDLG++ ++L+TNNPRKI GL+GY L Sbjct: 305 AYKLQEEGFDTVEANEKLGFPADLRDYGIGAQILKDLGLQHLRLLTNNPRKIAGLQGYDL 364 Query: 374 EVVERVPIQMPENKDNTNYLHTKQAKLGHLLTF 406 V ERVP+QMP ++N YL TK KLGHLL Sbjct: 365 TVTERVPLQMPAKEENKTYLQTKVNKLGHLLNL 397 >ref|NP_980474.1| 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II [Bacillus cereus ATCC 10987] gb|AAS43082.1| 3,4-dihydroxy-2-butanone 4-phosphate synthase/GTP cyclohydrolase II [Bacillus cereus ATCC 10987] Length = 397 Score = 592 bits (1527), Expect = e-168, Method: Compositional matrix adjust. Identities = 281/393 (71%), Positives = 330/393 (83%) Query: 14 IEDAIYDLMRGKVIIVVDDEDRENEGDFIALAERSTPEVINFMITEGRGLVCVPITAERA 73 IE+A+ DL +GKV+IV DDE+RENEGDFIALAE TPE INFMIT GRGLVCVPIT A Sbjct: 5 IEEALEDLKQGKVVIVCDDENRENEGDFIALAEYITPETINFMITHGRGLVCVPITEGYA 64 Query: 74 AELDLQPMVAHNTDNHGTAFTVSVDHKDTTTGISAAERSKTIQALMDPEAKPSDFRRPGH 133 L L+PMVAHNTD+H TAFTVS+DH TTTGISA ER+ TI+ +++P +K +DF RPGH Sbjct: 65 ERLQLEPMVAHNTDSHHTAFTVSIDHVSTTTGISAHERATTIREMLNPASKGADFNRPGH 124 Query: 134 MFPLIAKKGGVLRRSGHTEAAVDLARMCGAYPASVICEVVKEDGTMARLPDLVEIARKHE 193 +FPLIAK+GGVLRR+GHTEAAVDLA++CGA PA VICE++ EDGTMAR+PDL++ A++ + Sbjct: 125 IFPLIAKEGGVLRRAGHTEAAVDLAQLCGAEPAGVICEIINEDGTMARVPDLLQCAKQFD 184 Query: 194 LKLISIKDLIHYRNEKEQLVTREVSVKLPTDFGDFQTIAYTNEVDDKEHVALVKGDISGE 253 +K+I+I+DLI YR E LVTREV + LPTDFG F I Y+N +D KEH+ALVKGDIS Sbjct: 185 IKMITIEDLIAYRRHHETLVTREVEITLPTDFGTFHAIGYSNSLDTKEHIALVKGDISTG 244 Query: 254 EPVLVRVHSECLTGDVFHSHRCDCGPQFEAALRQIEAAGRGVLLYMRQEGRGIGLINKLR 313 EPVLVRVHSECLTGDVF S RCDCGPQ AAL QIE G+GVLLYMRQEGRGIGL+NKLR Sbjct: 245 EPVLVRVHSECLTGDVFGSCRCDCGPQLHAALAQIEREGKGVLLYMRQEGRGIGLLNKLR 304 Query: 314 AYKLQEEGLDTVDANLKLGFPADLRDYGIGAQILKDLGVRQIKLMTNNPRKIKGLEGYGL 373 AYKLQEEG DTV+AN KLGFPADLRDYGIGAQILKDLG++ ++L+TNNPRKI GL+GY L Sbjct: 305 AYKLQEEGFDTVEANEKLGFPADLRDYGIGAQILKDLGLQHLRLLTNNPRKIAGLQGYDL 364 Query: 374 EVVERVPIQMPENKDNTNYLHTKQAKLGHLLTF 406 EV+ERVP+QMP ++N YL TK KLGHLL Sbjct: 365 EVIERVPLQMPAKEENKTYLQTKVNKLGHLLNL 397 >ref|NP_390207.1| GTP cyclohydrolase II and 3,4-dihydroxy-2-butanone 4-phosphate synthase [Bacillus subtilis] sp|P17620|GCH2_BACSU Riboflavin biosynthesis protein ribA [Includes: GTP cyclohydrolase II ; 3,4-dihydroxy-2-butanone 4-phosphate synthase (DHBP synthase)] pir||S45545 GTP cyclohydrolase II (EC 3.5.4.25) / 3, 4-dihydroxy-2-butanone 4-phosphate synthase (EC 5.4.99.-) ribA - Bacillus subtilis emb|CAA35880.1| unnamed protein product [Bacillus subtilis] gb|AAA67483.1| ribA gene product emb|CAB14258.1| GTP cyclohydrolase II and 3,4-dihydroxy-2-butanone 4-phosphate synthase [Bacillus subtilis subsp. subtilis str. 168] Length = 398 Score = 588 bits (1515), Expect = e-166, Method: Compositional matrix adjust. Identities = 282/398 (70%), Positives = 328/398 (82%), Gaps = 1/398 (0%) Query: 10 VLDPIEDAIYDLMRGKVIIVVDDEDRENEGDFIALAERSTPEVINFMITEGRGLVCVPIT 69 + PIE+A+ L +G+VIIVVDDEDRENEGDF+ALAE +TPEVINFM T GRGL+C P++ Sbjct: 1 MFHPIEEALDALKKGEVIIVVDDEDRENEGDFVALAEHATPEVINFMATHGRGLICTPLS 60 Query: 70 AERAAELDLQPMVAHNTDNHGTAFTVSVDHKDTTTGISAAERSKTIQALMDPEAKPSDFR 129 E A LDL PMV HNTD+H TAFTVS+DH++T TGISA ERS T+QAL+D ++ PSDF+ Sbjct: 61 EEIADRLDLHPMVEHNTDSHHTAFTVSIDHRETKTGISAQERSFTVQALLDSKSVPSDFQ 120 Query: 130 RPGHMFPLIAKKGGVLRRSGHTEAAVDLARMCGAYPASVICEVVKEDGTMARLPDLVEIA 189 RPGH+FPLIAKKGGVL+R+GHTEAAVDLA CG+ A VICE++ EDGTMAR+P+L+EIA Sbjct: 121 RPGHIFPLIAKKGGVLKRAGHTEAAVDLAEACGSPGAGVICEIMNEDGTMARVPELIEIA 180 Query: 190 RKHELKLISIKDLIHYRNEKEQLVTREVSVKLPTDFGDFQTIAYTNEVDDKEHVALVKGD 249 +KH+LK+I+IKDLI YR LV REV + LPTDFG F+ YTNEVD KEHVA V GD Sbjct: 181 KKHQLKMITIKDLIQYRYNLTTLVEREVDITLPTDFGTFKVYGYTNEVDGKEHVAFVMGD 240 Query: 250 IS-GEEPVLVRVHSECLTGDVFHSHRCDCGPQFEAALRQIEAAGRGVLLYMRQEGRGIGL 308 + GEEPVLVRVHSECLTGDVF SHRCDCGPQ AAL QI A GRGVLLY+RQEGRGIGL Sbjct: 241 VPFGEEPVLVRVHSECLTGDVFGSHRCDCGPQLHAALNQIAAEGRGVLLYLRQEGRGIGL 300 Query: 309 INKLRAYKLQEEGLDTVDANLKLGFPADLRDYGIGAQILKDLGVRQIKLMTNNPRKIKGL 368 INKL+AYKLQE+G DTV+AN LGF DLR+YGIGAQIL+DLGVR +KL+TNNPRKI GL Sbjct: 301 INKLKAYKLQEQGYDTVEANEALGFLPDLRNYGIGAQILRDLGVRNMKLLTNNPRKIAGL 360 Query: 369 EGYGLEVVERVPIQMPENKDNTNYLHTKQAKLGHLLTF 406 EGYGL + ERVP+QM + N YL TK KLGHLL F Sbjct: 361 EGYGLSISERVPLQMEAKEHNKKYLQTKMNKLGHLLHF 398 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.137 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 619,737,844 Number of extensions: 26205742 Number of successful extensions: 68736 Number of sequences better than 1.0e-05: 1028 Number of HSP's gapped: 67486 Number of HSP's successfully gapped: 1028 Length of query: 417 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 289 Effective length of database: 409,704,906 Effective search space: 118404717834 Effective search space used: 118404717834 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)