BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0027.p (172 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_390828.1| ytfJ [Bacillus subtilis] >gi|7475394|pir||H6999... 149 3e-35 ref|NP_244061.1| BH3195~unknown conserved protein in B. subtilis... 148 5e-35 ref|NP_844500.1| conserved hypothetical protein [Bacillus anthra... 141 5e-33 ref|NP_655956.1| hypothetical protein predicted by GeneMark [Bac... 139 3e-32 ref|NP_831863.1| hypothetical protein [Bacillus cereus ATCC 1457... 136 2e-31 >ref|NP_390828.1| ytfJ [Bacillus subtilis] pir||H69991 hypothetical protein ytfJ - Bacillus subtilis gb|AAC00315.1| YtfJ [Bacillus subtilis] emb|CAB14928.1| ytfJ [Bacillus subtilis subsp. subtilis str. 168] Length = 151 Score = 149 bits (376), Expect = 3e-35, Method: Compositional matrix adjust. Identities = 76/137 (55%), Positives = 96/137 (70%), Gaps = 14/137 (10%) Query: 1 MSEHPIQGLMQTAMENIKGMVDVNTIVGDPVETPDGSVILPISKVAFGFAAGGSDY---- 56 M++HPIQGLM+TAMEN+K M+DVNTI+GDPVETPDGSVIL +SKV FGFAAGGS++ Sbjct: 1 MADHPIQGLMKTAMENLKEMIDVNTIIGDPVETPDGSVILTVSKVGFGFAAGGSEFGGKP 60 Query: 57 ---RVEDDAPGINGAGSGVKMLPFXXXXXXXVSIRPIAFLVVGREGVHIVPLDNQTHLFE 113 + EDD + + LPF VSI PIAFL+VG G+ ++ LD THL E Sbjct: 61 AEKKSEDDE-------TREQKLPFGGGSGGGVSITPIAFLIVGSTGIRMLHLDENTHLIE 113 Query: 114 KIIDATPGLIDKIQTMF 130 KI+DA P +++IQ MF Sbjct: 114 KILDAAPQTLERIQQMF 130 >ref|NP_244061.1| BH3195~unknown conserved protein in B. subtilis [Bacillus halodurans] pir||C84049 hypothetical protein BH3195 [imported] - Bacillus halodurans (strain C-125) dbj|BAB06914.1| BH3195~unknown conserved protein in B. subtilis [Bacillus halodurans] Length = 147 Score = 148 bits (374), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 78/130 (60%), Positives = 94/130 (72%), Gaps = 6/130 (4%) Query: 1 MSEHPIQGLMQTAMENIKGMVDVNTIVGDPVETPDGSVILPISKVAFGFAAGGSDYRVED 60 MS+HPIQGLM+TAMEN+K MVDVNTIVGDPVETPDGSVI+P+SKV FGFAAGGS++ V Sbjct: 1 MSDHPIQGLMKTAMENLKEMVDVNTIVGDPVETPDGSVIMPVSKVGFGFAAGGSEFMV-- 58 Query: 61 DAPGINGAGSGVKMLPFXXXXXXXVSIRPIAFLVVGREGVHIVPLDNQTHLFEKIIDATP 120 D G + S PF VSI PIAFLVV G+ +V +D THL+EK++D P Sbjct: 59 DQIGDQHSASH----PFGGGSGGGVSITPIAFLVVNSHGIKMVHIDGSTHLYEKLVDFAP 114 Query: 121 GLIDKIQTMF 130 +I+KIQ M Sbjct: 115 QVIEKIQQML 124 >ref|NP_844500.1| conserved hypothetical protein [Bacillus anthracis str. Ames] ref|NP_978494.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] gb|AAP25986.1| conserved hypothetical protein [Bacillus anthracis str. Ames] gb|AAS41102.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] Length = 141 Score = 141 bits (356), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 75/142 (52%), Positives = 96/142 (67%), Gaps = 7/142 (4%) Query: 3 EHPIQGLMQTAMENIKGMVDVNTIVGDPVETPDGSVILPISKVAFGFAAGGSDYRVEDDA 62 +HPIQGLM+ AMEN+K MVDVNTIVG+PV+T DG V+L +SKVAFGF AGGSD++ +D Sbjct: 2 DHPIQGLMKAAMENLKEMVDVNTIVGEPVQTADGGVVLTVSKVAFGFGAGGSDFQT-NDG 60 Query: 63 PGINGAGSGVKMLPFXXXXXXXVSIRPIAFLVVGREGVHIVPLDNQTHLFEKIIDATPGL 122 NG + F VSI P+AFLVV ++GV+I+ L N THL EK+I+ P Sbjct: 61 QRANGNPA------FGGGSAGGVSITPVAFLVVNKDGVNILHLQNATHLAEKMIELAPQT 114 Query: 123 IDKIQTMFPSSGSSQNDASPQN 144 IDKIQ+MF + + PQN Sbjct: 115 IDKIQSMFQKDEKKEGNHHPQN 136 >ref|NP_655956.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] Length = 141 Score = 139 bits (349), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 74/147 (50%), Positives = 94/147 (63%), Gaps = 17/147 (11%) Query: 3 EHPIQGLMQTAMENIKGMVDVNTIVGDPVETPDGSVILPISKVAFGFAAGGSDYRVEDDA 62 +HPIQ LM+ AMEN+K MVDVNTIVG+PV+T DG V+L +SKVAFGF AGGSD++ D Sbjct: 2 DHPIQXLMKAAMENLKEMVDVNTIVGEPVQTADGGVVLTVSKVAFGFGAGGSDFQTNDGQ 61 Query: 63 -----PGINGAGSGVKMLPFXXXXXXXVSIRPIAFLVVGREGVHIVPLDNQTHLFEKIID 117 P G +G VSI P+AFLVV ++GV+I+ L N THL EK+I+ Sbjct: 62 RANGNPAFGGGSAG------------GVSITPVAFLVVNKDGVNILHLQNATHLAEKMIE 109 Query: 118 ATPGLIDKIQTMFPSSGSSQNDASPQN 144 P IDKIQ+MF + + PQN Sbjct: 110 LAPQTIDKIQSMFQKDEKKEGNHHPQN 136 >ref|NP_831863.1| hypothetical protein [Bacillus cereus ATCC 14579] gb|AAP09064.1| hypothetical protein [Bacillus cereus ATCC 14579] Length = 142 Score = 136 bits (343), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 75/148 (50%), Positives = 94/148 (63%), Gaps = 18/148 (12%) Query: 3 EHPIQGLMQTAMENIKGMVDVNTIVGDPVETPDGSVILPISKVAFGFAAGGSDYRVEDDA 62 +HPIQGLM+ AMEN+K MVDVNTIVG+PV+T DG V+L +SKVAFGF AGGSD++ D Sbjct: 2 DHPIQGLMKAAMENLKEMVDVNTIVGEPVQTADGGVVLTVSKVAFGFGAGGSDFQTNDGQ 61 Query: 63 -----PGINGAGSGVKMLPFXXXXXXXVSIRPIAFLVVGREGVHIVPLDNQTHLFEKIID 117 P G +G VSI P+AFLVV ++GV+I+ L N THL EK+I+ Sbjct: 62 RANGNPAFGGGSAG------------GVSITPVAFLVVNKDGVNILHLQNATHLAEKMIE 109 Query: 118 ATPGLIDKIQTMFPSSGSSQ-NDASPQN 144 P IDKIQ+MF + N QN Sbjct: 110 LAPQTIDKIQSMFQKEEKKEGNHHHHQN 137 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 259,728,307 Number of extensions: 10805202 Number of successful extensions: 19034 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 19009 Number of HSP's successfully gapped: 21 Length of query: 172 Length of database: 757,237,578 Length adjustment: 117 Effective length of query: 55 Effective length of database: 439,570,995 Effective search space: 24176404725 Effective search space used: 24176404725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 121 (51.2 bits)