BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0026.p (400 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_692747.1| D-alanyl-D-alanine carboxypeptidase [Oceanobaci... 270 5e-71 ref|NP_831248.1| D-alanyl-D-alanine carboxypeptidase [Bacillus c... 268 1e-70 ref|NP_977915.1| D-alanyl-D-alanine carboxypeptidase family prot... 265 2e-69 ref|NP_390200.1| D-alanyl-D-alanine carboxypeptidase (penicillin... 260 3e-68 ref|NP_843943.1| D-alanyl-D-alanine carboxypeptidase family prot... 260 4e-68 >ref|NP_692747.1| D-alanyl-D-alanine carboxypeptidase [Oceanobacillus iheyensis HTE831] dbj|BAC13782.1| D-alanyl-D-alanine carboxypeptidase (penicilin binding protein) [Oceanobacillus iheyensis HTE831] Length = 367 Score = 270 bits (690), Expect = 5e-71, Method: Compositional matrix adjust. Identities = 136/266 (51%), Positives = 186/266 (69%), Gaps = 7/266 (2%) Query: 37 TTHARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKNAFAK 96 + A A LI+ ++GR+L+ +E IAS+TKIMTA++AIE+G L +V + A Sbjct: 27 SVSANNAVLIEQSTGRVLFEKNANEEASIASITKIMTAIIAIESGMLDEQVTTSRKAIYT 86 Query: 97 EGSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEELGLQH 156 EGSS++L+QGEE+ L+D++YGLMLRSGNDAA AIAEHVGGSEEGFVYLMN KA+ LG+++ Sbjct: 87 EGSSIYLEQGEEIPLKDLVYGLMLRSGNDAAVAIAEHVGGSEEGFVYLMNEKAKWLGMEN 146 Query: 157 THFTNPHGLDAEGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYKWLNKN 216 THF NPHGLD++ HYSSA D+A+L YAM NP+F+++ ++ ++N Y W NKN Sbjct: 147 THFDNPHGLDSDTHYSSAYDMAILMKYAMDNPMFREVSQSDSYLSNNR----TYHWQNKN 202 Query: 217 KML-RLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLDYGFNH 275 K+L RLYE G KTGYTK R LV+SA + G L+AVTL+ +DW DH + ++GF Sbjct: 203 KLLTRLYEYCTGGKTGYTKATGRTLVTSAHKNGMDLIAVTLDAPDDWQDHIGMFEWGFEQ 262 Query: 276 YPLKTLVERGEGISGYSFVPARTFAY 301 + +KT+ E EG + Y P + Y Sbjct: 263 FEMKTVQE--EGTALYHIEPKTSKVY 286 >ref|NP_831248.1| D-alanyl-D-alanine carboxypeptidase [Bacillus cereus ATCC 14579] gb|AAP08449.1| D-alanyl-D-alanine carboxypeptidase [Bacillus cereus ATCC 14579] Length = 386 Score = 268 bits (686), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 150/331 (45%), Positives = 214/331 (64%), Gaps = 19/331 (5%) Query: 33 NSTITTHARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKN 92 NS ++ AR A L++ SGR+LY EP IAS+TKIMTAL+A E+G ++ V V Sbjct: 39 NSNVS--ARNAVLMEQQSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKEMVSVSNE 96 Query: 93 AFAKEGSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEEL 152 A EGS+++LK G++++LED++YGLMLRSGNDAA IAE VGGS +GFVYLMN KA+E+ Sbjct: 97 AVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAESVGGSIDGFVYLMNQKAKEI 156 Query: 153 GLQHTHFTNPHGLDAEG-HYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYK 211 G++ THF+NPHGLD +G HYSSA D+A+LT YAM N FK+I T+ +D+ WDY Sbjct: 157 GMKDTHFSNPHGLDGDGSHYSSAYDMALLTKYAMGNETFKKIFGTKTYKSDS----WDYP 212 Query: 212 WLNKNKML-RLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLD 270 W NK+K++ YE A G KTG+TKKA R LV++A++GG L+ VTL+ +DW+DH L D Sbjct: 213 WKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKGGLDLIVVTLSASSDWDDHMNLFD 272 Query: 271 YGFNHYPLKTLVERG------EGISGYSFVPARTFAYPLGQGEEARLVTKLELIRENKNT 324 GF+ + ++ +G E +F+ PL + E+ +V K+EL + K T Sbjct: 273 KGFDRFKQTKVLGQGAIAEINEKKYANHVYTKNSFSVPLTEEEKKNVVLKVELDKSTKLT 332 Query: 325 RRSSFGIK-GALVLQLGGKEIGRVPVYMPDR 354 G+K G + +G +++G ++ R Sbjct: 333 D----GVKVGKTDIYVGNEKVGERNLFYSKR 359 >ref|NP_977915.1| D-alanyl-D-alanine carboxypeptidase family protein [Bacillus cereus ATCC 10987] gb|AAS40523.1| D-alanyl-D-alanine carboxypeptidase family protein [Bacillus cereus ATCC 10987] Length = 374 Score = 265 bits (676), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 149/329 (45%), Positives = 208/329 (63%), Gaps = 17/329 (5%) Query: 35 TITTHARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKNAF 94 T AR A L++ SGR+LY EP IAS+TKIMTAL+A E+G ++ V V A Sbjct: 27 TSNVSARNAVLMEQQSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKEMVSVSNEAV 86 Query: 95 AKEGSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEELGL 154 EGS+++LK G++++LED++YGLMLRSGNDAA IAE+VGGS EGFVYLMN KA+++G+ Sbjct: 87 RVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQIGM 146 Query: 155 QHTHFTNPHGLDAEG-HYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYKWL 213 + THF+NPHGLD +G HYSSA D+A+LT YAM N FK+I T+ +D+ WDY W Sbjct: 147 KDTHFSNPHGLDGDGSHYSSAYDMALLTRYAMGNETFKKIFGTKTYKSDS----WDYPWK 202 Query: 214 NKNKML-RLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLDYG 272 NK+K++ YE A G KTG+TKKA R LV++A++ G L+ VTL+ +DW+DH L D G Sbjct: 203 NKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLDLIVVTLSASSDWDDHMNLFDKG 262 Query: 273 FNHYPLKTLVERG------EGISGYSFVPARTFAYPLGQGEEARLVTKLELIRENKNTRR 326 F + +V +G E +FA PL + E +V K+EL + K Sbjct: 263 FERFKQTNVVGQGALAEITEKKYANHVYTKNSFAVPLTEQERKNVVLKVELDKSAKLKD- 321 Query: 327 SSFGIK-GALVLQLGGKEIGRVPVYMPDR 354 G+K G + +G +++G ++ R Sbjct: 322 ---GVKVGKTEIYVGNEKVGERNLFYSKR 347 >ref|NP_390200.1| D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5*) [Bacillus subtilis] sp|P35150|DACB_BACSU Penicillin-binding protein 5* precursor (D-alanyl-D-alanine carboxypeptidase) (DD-peptidase) (DD-carboxypeptidase) (PBP-5*) pir||S45552 serine-type D-Ala-D-Ala carboxypeptidase (EC 3.4.16.4) dacB precursor - Bacillus subtilis gb|AAA67490.1| penicillin-binding protein emb|CAB14251.1| D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 5*) [Bacillus subtilis subsp. subtilis str. 168] Length = 382 Score = 260 bits (665), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 146/347 (42%), Positives = 218/347 (62%), Gaps = 19/347 (5%) Query: 33 NSTITTHARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKN 92 ++ I A++A +ID ASGR+LY+ + IAS+TKIMTA++AIE+G + V V N Sbjct: 26 HAAIDVSAKSAIIIDGASGRVLYAKDEHQKRRIASITKIMTAVLAIESGKMDQTVTVSAN 85 Query: 93 AFAKEGSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEEL 152 A EGS+++L +G++++L+D++YGLMLRSGNDAA AIAEHVGGS +GFVY+MN KAE+L Sbjct: 86 AVRTEGSAIYLTEGQKVKLKDLVYGLMLRSGNDAAVAIAEHVGGSLDGFVYMMNQKAEQL 145 Query: 153 GLQHTHFTNPHGLDA-EGHYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYK 211 G+++T F NPHGLD E HYS+A D+A+LT YAM +++I T++ A E + Sbjct: 146 GMKNTRFQNPHGLDDHENHYSTAYDMAILTKYAMKLKDYQKISGTKIYKA----ETMESV 201 Query: 212 WLNKNKMLR-LYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLD 270 W NKNK+L LY + G KTGYTK A R LVS+A++ G L+AVT+ND NDW+DH + + Sbjct: 202 WKNKNKLLTMLYPYSTGGKTGYTKLAKRTLVSTASKDGIDLIAVTINDPNDWDDHMKMFN 261 Query: 271 YGFNHYPLKTLVERGE--GISGYSFVPARTF-----AYPLGQGEEARLVTKLELIRENKN 323 Y F HY + ++G+ + G +F ++ F Y L + E+ + L++ K Sbjct: 262 YVFEHYQTYLIAKKGDIPKLKG-TFYESKAFIKRDITYLLTEEEKENVKINTTLLKPKKA 320 Query: 324 TRRSSFGIK---GALVLQLGGKEIGRVPVYMPDRLPPEQSPYEKRFQ 367 + + I G + + I +VP+Y + Q P ++ F+ Sbjct: 321 WEKDASKIPDIVGHMEIMFNDATIAKVPIYYENE--RHQKPKKQFFE 365 >ref|NP_843943.1| D-alanyl-D-alanine carboxypeptidase family protein [Bacillus anthracis str. Ames] gb|AAP25429.1| D-alanyl-D-alanine carboxypeptidase family protein [Bacillus anthracis str. Ames] Length = 374 Score = 260 bits (664), Expect = 4e-68, Method: Compositional matrix adjust. Identities = 149/342 (43%), Positives = 215/342 (62%), Gaps = 30/342 (8%) Query: 33 NSTITTHARAAALIDVASGRLLYSSRGDEPMLIASLTKIMTALVAIENGDLRSKVKVGKN 92 NS ++ AR A L++ SGR+LY EP IAS+TKIMTAL+A E+G ++ +V V Sbjct: 27 NSNVS--ARNAVLMEQHSGRVLYGKAEHEPQKIASITKIMTALLAAESGKMKERVLVSNE 84 Query: 93 AFAKEGSSLFLKQGEEMRLEDMLYGLMLRSGNDAATAIAEHVGGSEEGFVYLMNAKAEEL 152 A EGS+++LK G++++LED++YGLMLRSGNDAA IAE+VGGS EGFVYLMN KA+++ Sbjct: 85 AVRVEGSAIYLKPGQKVKLEDLVYGLMLRSGNDAAQVIAENVGGSIEGFVYLMNEKAKQI 144 Query: 153 GLQHTHFTNPHGLDAEG-HYSSANDLAVLTAYAMHNPVFKQIVKTQVKTADNPYEPWDYK 211 ++ THF+NPHGLD +G HYSSA D+A+LT YAM N FK+I T+ +D+ WDY Sbjct: 145 RMKDTHFSNPHGLDGDGSHYSSAYDMALLTRYAMGNETFKEIFGTKTYKSDS----WDYP 200 Query: 212 WLNKNKML-RLYEGADGVKTGYTKKALRCLVSSATRGGQQLVAVTLNDGNDWNDHAVLLD 270 W NK+K++ YE A G KTG+TKKA R LV++A++ G L+ VTL+ +DW+DH L D Sbjct: 201 WKNKHKLVTSYYEFATGGKTGFTKKAGRTLVTTASKDGLDLIVVTLSASSDWDDHMNLFD 260 Query: 271 YGFNHYPLKTLVERG------EGISGYSFVPARTFAYPLGQGEEARLVTKLELIRENKNT 324 GF+ + ++ +G E +F+ PL + E+ +V K+EL + K Sbjct: 261 KGFDRFEQTKVLGQGALAEITEKKYANHVYTKNSFSVPLTEEEKKSVVLKVELDKSAK-- 318 Query: 325 RRSSFGIKGALVLQLGGKEIGRVPVYMPDRLPPEQSP-YEKR 365 + G ++G+ VY+ + E++ Y KR Sbjct: 319 -------------LVDGVKVGKTDVYVGNEKVGERNLFYSKR 347 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.132 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 549,372,736 Number of extensions: 22673672 Number of successful extensions: 54043 Number of sequences better than 1.0e-05: 681 Number of HSP's gapped: 52935 Number of HSP's successfully gapped: 681 Length of query: 400 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 272 Effective length of database: 409,704,906 Effective search space: 111439734432 Effective search space used: 111439734432 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)