BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0015.p (482 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|Q9X1E4|GLK2_THEMA Glycerol kinase 2 (ATP:glycerol 3-phosphotr... 557 e-157 ref|NP_623576.1| Glycerol kinase [Thermoanaerobacter tengcongens... 537 e-151 ref|NP_229230.1| glycerol kinase [Thermotoga maritima] >gi|74342... 536 e-151 ref|NP_782352.1| glycerol kinase [Clostridium tetani E88] >gi|28... 523 e-147 ref|NP_457966.1| glycerol kinase [Salmonella enterica subsp. ent... 522 e-147 >sp|Q9X1E4|GLK2_THEMA Glycerol kinase 2 (ATP:glycerol 3-phosphotransferase 2) (Glycerokinase 2) (GK 2) Length = 496 Score = 557 bits (1436), Expect = e-157, Method: Compositional matrix adjust. Identities = 265/479 (55%), Positives = 339/479 (70%), Gaps = 1/479 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +FD++ +VS+ E RQ +PRPGWVEHDP EIWE+Q+ A+ AI E+G +I+AIGI Sbjct: 18 VFDEKGNVVSKVNKEFRQIYPRPGWVEHDPVEIWESQIEVAKKAIEEAGIKPEDIAAIGI 77 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWDK TGKP+Y AIVWQ RRTA C++LK +G ++ I +TGLV+DAYFS T Sbjct: 78 TNQRETTIVWDKNTGKPVYNAIVWQCRRTAPICDELKEKGYSEFIRERTGLVIDAYFSGT 137 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD+V G R +AE+GE+L GT+D WLIW LTGG VH TD +NASRTM++N+H+ W Sbjct: 138 KIKWILDNVEGVREKAEKGEVLFGTVDTWLIWNLTGGRVHVTDYSNASRTMIFNIHKLDW 197 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D +IPRA+LP+V S +G + FGVEIPI GDQQAALFG C + G Sbjct: 198 DDEILELLNIPRAMLPQVMPSSHVYGYTAKDIFGVEIPIAGDAGDQQAALFGQACFQPGM 257 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEGL 300 KNTYGTGCF+LMNTG +A S GLLTT+AWG+ ++YYALEGS+F+ GAAVQWL +GL Sbjct: 258 LKNTYGTGCFLLMNTGEKAFESKSGLLTTIAWGINGKVYYALEGSIFITGAAVQWLRDGL 317 Query: 301 GVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRAT 360 +I A++EE A PAF GLGAPYWDMYARG + G+TRGTT H+VRA Sbjct: 318 KIISNAAETEELATKVPDNGGVFFVPAFVGLGAPYWDMYARGLIIGITRGTTREHIVRAV 377 Query: 361 LESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXXX 420 LES+A+Q+RDV+ MEKD+ + + LRVDGGAV N+ LMQFQADILG V R + E Sbjct: 378 LESIAYQTRDVVEVMEKDSDIKVETLRVDGGAVVNNFLMQFQADILGVPVERPVVNETTA 437 Query: 421 XXXXXXXXXXSGIW-NREQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGWEKH 478 G W ++E++ S + ++ F PSM +EERER Y W++AVSR++GWEK Sbjct: 438 LGAAYLAGLAVGYWKDQEEIASLWQLDRRFEPSMNSEERERLYSKWKEAVSRSLGWEKR 496 >ref|NP_623576.1| Glycerol kinase [Thermoanaerobacter tengcongensis] sp|Q8R8J4|GLPK_THETN Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase) (GK) gb|AAM25180.1| Glycerol kinase [Thermoanaerobacter tengcongensis] Length = 497 Score = 537 bits (1384), Expect = e-151, Method: Compositional matrix adjust. Identities = 259/476 (54%), Positives = 321/476 (67%), Gaps = 1/476 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +FD M++ E RQ +P+PGWVEHDP EIWE+Q+ A+ I ++G +I+AIGI Sbjct: 19 IFDHSGKMIASLNKEFRQIYPKPGWVEHDPMEIWESQIEVAKGVIEKAGIKPEDIAAIGI 78 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWDK TGKPIY AIVWQ RRTA C+ LK +G I KTGLVVDAYFS T Sbjct: 79 TNQRETTVVWDKNTGKPIYNAIVWQCRRTAPICDDLKNKGFDKKIREKTGLVVDAYFSGT 138 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD+V GAR +AE+GELL G ID WLIW LT G VH TD +NASRTML+N+HE +W Sbjct: 139 KVKWILDNVEGAREKAERGELLFGNIDTWLIWNLTRGKVHVTDYSNASRTMLFNIHELKW 198 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D ++P +LPEV+ S +G FGVEIPI GDQQAALFG C + G Sbjct: 199 DKEILEELNVPENMLPEVKPSSHVYGYTDKSIFGVEIPIAGDAGDQQAALFGQACFKPGM 258 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEGL 300 AKNTYGTGCF+LMNTG +AV S GLLTT+AWG+ ++ YALEGS+F+ GAA+QWL + L Sbjct: 259 AKNTYGTGCFMLMNTGEKAVPSKTGLLTTIAWGIDGKVEYALEGSIFITGAAIQWLRDEL 318 Query: 301 GVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRAT 360 +I+ SEE A PAF GLGAPYWDMYARG + GLTRG H++RAT Sbjct: 319 RIIDNAPQSEEYALKVEDTNGVYVVPAFVGLGAPYWDMYARGVIVGLTRGAKREHIIRAT 378 Query: 361 LESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXXX 420 LES+A+Q+RDV+ AM++D+G+ L L+VDGGA N+ LMQFQADILG V R E Sbjct: 379 LESIAYQTRDVLEAMQEDSGIKLQALKVDGGASANNFLMQFQADILGVPVDRPQVIETTA 438 Query: 421 XXXXXXXXXXSGIWN-REQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGW 475 G WN RE++E ++ F P+ME E+RE+ Y W+ AV R M W Sbjct: 439 LGAAYLAGLAVGFWNSREEIEKNWNIDRRFEPAMEEEKREKLYRGWKKAVERAMKW 494 >ref|NP_229230.1| glycerol kinase [Thermotoga maritima] pir||C72254 glycerol kinase - Thermotoga maritima (strain MSB8) gb|AAD36500.1| glycerol kinase [Thermotoga maritima] Length = 482 Score = 536 bits (1380), Expect = e-151, Method: Compositional matrix adjust. Identities = 256/463 (55%), Positives = 326/463 (70%), Gaps = 1/463 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +FD++ +VS+ E RQ +PRPGWVEHDP EIWE+Q+ A+ AI E+G +I+AIGI Sbjct: 18 VFDEKGNVVSKVNKEFRQIYPRPGWVEHDPVEIWESQIEVAKKAIEEAGIKPEDIAAIGI 77 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWDK TGKP+Y AIVWQ RRTA C++LK +G ++ I +TGLV+DAYFS T Sbjct: 78 TNQRETTIVWDKNTGKPVYNAIVWQCRRTAPICDELKEKGYSEFIRERTGLVIDAYFSGT 137 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD+V G R +AE+GE+L GT+D WLIW LTGG VH TD +NASRTM++N+H+ W Sbjct: 138 KIKWILDNVEGVREKAEKGEVLFGTVDTWLIWNLTGGRVHVTDYSNASRTMIFNIHKLDW 197 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D +IPRA+LP+V S +G + FGVEIPI GDQQAALFG C + G Sbjct: 198 DDEILELLNIPRAMLPQVMPSSHVYGYTAKDIFGVEIPIAGDAGDQQAALFGQACFQPGM 257 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEGL 300 KNTYGTGCF+LMNTG +A S GLLTT+AWG+ ++YYALEGS+F+ GAAVQWL +GL Sbjct: 258 LKNTYGTGCFLLMNTGEKAFESKSGLLTTIAWGINGKVYYALEGSIFITGAAVQWLRDGL 317 Query: 301 GVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRAT 360 +I A++EE A PAF GLGAPYWDMYARG + G+TRGTT H+VRA Sbjct: 318 KIISNAAETEELATKVPDNGGVFFVPAFVGLGAPYWDMYARGLIIGITRGTTREHIVRAV 377 Query: 361 LESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXXX 420 LES+A+Q+RDV+ MEKD+ + + LRVDGGAV N+ LMQFQADILG V R + E Sbjct: 378 LESIAYQTRDVVEVMEKDSDIKVETLRVDGGAVVNNFLMQFQADILGVPVERPVVNETTA 437 Query: 421 XXXXXXXXXXSGIW-NREQLESFNKAEKIFAPSMEAEERERRY 462 G W ++E++ S + ++ F PSM +EERER Y Sbjct: 438 LGAAYLAGLAVGYWKDQEEIASLWQLDRRFEPSMNSEERERLY 480 >ref|NP_782352.1| glycerol kinase [Clostridium tetani E88] gb|AAO36289.1| glycerol kinase [Clostridium tetani E88] Length = 498 Score = 523 bits (1346), Expect = e-147, Method: Compositional matrix adjust. Identities = 256/480 (53%), Positives = 325/480 (67%), Gaps = 1/480 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 +F++ +VS Q E +Q +P+ GWVEHDP EIW TQ + A A++++ A+EI++IGI Sbjct: 19 IFNERGLIVSVAQREFKQIYPKGGWVEHDPMEIWATQFSVATEAMAKANIEASEIASIGI 78 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRET +VWDK TG P+Y AIVWQ RRTA+ C++LK +GL +TI KTGLV+DAYFS T Sbjct: 79 TNQRETTIVWDKRTGLPVYNAIVWQCRRTAQICDELKEKGLTETIRNKTGLVLDAYFSGT 138 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILD+V GAR AE+G L+ GT+D WLIW LT G VH TD +NASRTM+YN+HE +W Sbjct: 139 KIKWILDNVAGAREEAEKGNLIFGTVDTWLIWNLTKGKVHVTDYSNASRTMIYNIHELKW 198 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAGSEWFGVEIPIRSVLGDQQAALFGHTCLEAGS 240 D IP+++LPEV+ S +G S FG IPI V GDQQAALFG C + G+ Sbjct: 199 DDELLEALDIPKSMLPEVKPSSYVYGETNSTLFGSSIPIAGVAGDQQAALFGQMCHQEGT 258 Query: 241 AKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEGL 300 AK+TYGTGCF+LMNTG +AV S +GLLTT+A+G+ D++ YALEGS+F+ GAA+QWL + L Sbjct: 259 AKSTYGTGCFLLMNTGEKAVKSENGLLTTIAFGIDDKVEYALEGSIFIGGAAIQWLRDEL 318 Query: 301 GVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRAT 360 +++ +SE A PAF GLGAPYWD YARGA+ GLTRG T H +RAT Sbjct: 319 RMLKDSPESERYATAVEDTNGVYMVPAFVGLGAPYWDPYARGAIVGLTRGATKEHFIRAT 378 Query: 361 LESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXXX 420 LESLA+Q+ DV+ AM +D+G+ L LRVDGGA ND LMQFQADILG V R E Sbjct: 379 LESLAYQTYDVLNAMREDSGIDLKALRVDGGASANDFLMQFQADILGVPVQRPEVIETTA 438 Query: 421 XXXXXXXXXXSGIWNREQLESFNKA-EKIFAPSMEAEERERRYHAWQDAVSRTMGWEKHE 479 G W ++ + N A K F P M E RE W +AV R+M WEK E Sbjct: 439 LGAAYLAGLAVGYWKDKKDVAQNWAISKTFEPDMIKERREELLEGWHEAVKRSMNWEKSE 498 >ref|NP_457966.1| glycerol kinase [Salmonella enterica subsp. enterica serovar Typhi] ref|NP_807179.1| glycerol kinase [Salmonella enterica subsp. enterica serovar Typhi Ty2] sp|Q8Z2Y6|GLPK_SALTI Glycerol kinase (ATP:glycerol 3-phosphotransferase) (Glycerokinase) (GK) pir||AG0939 glycerol kinase [imported] - Salmonella enterica subsp. enterica serovar Typhi (strain CT18) emb|CAD09537.1| glycerol kinase [Salmonella enterica subsp. enterica serovar Typhi] gb|AAO71039.1| glycerol kinase [Salmonella enterica subsp. enterica serovar Typhi Ty2] Length = 501 Score = 522 bits (1345), Expect = e-147, Method: Compositional matrix adjust. Identities = 259/481 (53%), Positives = 325/481 (67%), Gaps = 2/481 (0%) Query: 1 LFDQEAGMVSQGQYEIRQSFPRPGWVEHDPDEIWETQLAAARAAISESGKPAAEISAIGI 60 + D +A +VS Q E Q +P+PGWVEHDP EIW +Q + ++++ + +I+AIGI Sbjct: 20 VMDHDANIVSVSQREFEQIYPKPGWVEHDPMEIWASQSSTLVEVLAKADISSDQIAAIGI 79 Query: 61 TNQRETALVWDKATGKPIYPAIVWQDRRTAEQCEQLKGRGLADTIAAKTGLVVDAYFSAT 120 TNQRETA+VW++ TGKPIY AIVWQ RRTA CEQLK GL D I TGLVVD YFS T Sbjct: 80 TNQRETAIVWERETGKPIYNAIVWQCRRTANICEQLKRDGLEDYIRDNTGLVVDPYFSGT 139 Query: 121 KLAWILDHVPGARARAEQGELLGGTIDAWLIWKLTGGAVHATDVTNASRTMLYNLHERRW 180 K+ WILDHV G+R RA++GELL GT+D WLIWK+T G VH TD TNASRTML+N+H+ W Sbjct: 140 KVKWILDHVEGSRERAKRGELLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHDLDW 199 Query: 181 DXXXXXXXSIPRAILPEVRMSGGDFGAAG-SEWFGVEIPIRSVLGDQQAALFGHTCLEAG 239 D IPRA+LP+VR S +G G IPI + GDQQAALFG C++ G Sbjct: 200 DDKMLDVLDIPRAMLPQVRKSSEVYGQTNIGGKGGTRIPIAGIAGDQQAALFGQLCVKEG 259 Query: 240 SAKNTYGTGCFILMNTGTEAVASSHGLLTTVAWGMGDELYYALEGSVFVAGAAVQWLHEG 299 AKNTYGTGCF+LMNTG +AV S +GLLTT+A G E+ YALEG+VF+AGA++QWL + Sbjct: 260 MAKNTYGTGCFMLMNTGEKAVKSENGLLTTIACGPSGEVNYALEGAVFMAGASIQWLRDE 319 Query: 300 LGVIEGPADSEEKAXXXXXXXXXXXXPAFTGLGAPYWDMYARGAVFGLTRGTTAGHLVRA 359 + +I DSE A PAFTGLGAPYWD YARGA+FGLTRG + H++RA Sbjct: 320 MKLISDAFDSEYFATKVKDTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNSNHIIRA 379 Query: 360 TLESLAFQSRDVIGAMEKDAGMPLTGLRVDGGAVRNDLLMQFQADILGSEVTRTMYAEXX 419 TLES+A+Q+RDV+ AM+ D+G+ L LRVDGGAV N+ LMQFQ+DILG+ V R E Sbjct: 380 TLESIAYQTRDVLEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVT 439 Query: 420 XXXXXXXXXXXSGIW-NREQLESFNKAEKIFAPSMEAEERERRYHAWQDAVSRTMGWEKH 478 G W N ++L+ E+ F P +E ER RY W+ AV R M WE+H Sbjct: 440 ALGAAYLAGLAVGYWQNLDELQEKAVIEREFRPGIETTERNYRYSGWKKAVKRAMAWEEH 499 Query: 479 E 479 + Sbjct: 500 D 500 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 671,194,101 Number of extensions: 25355522 Number of successful extensions: 61936 Number of sequences better than 1.0e-05: 890 Number of HSP's gapped: 60414 Number of HSP's successfully gapped: 904 Length of query: 482 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 352 Effective length of database: 404,274,708 Effective search space: 142304697216 Effective search space used: 142304697216 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)