BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0247.0011.p (510 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAI37884.1| unknown [environmental sequence] 243 6e-63 gb|EAI46301.1| unknown [environmental sequence] 241 2e-62 ref|ZP_00077013.1| COG0028: Thiamine pyrophosphate-requiring enz... 238 2e-61 ref|NP_618663.1| acetolactate synthase, large subunit [Methanosa... 235 2e-60 ref|NP_632694.1| Acetolactate synthase large subunit [Methanosar... 228 2e-58 >gb|EAI37884.1| unknown [environmental sequence] Length = 559 Score = 243 bits (621), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 166/506 (32%), Positives = 250/506 (49%), Gaps = 29/506 (5%) Query: 12 RHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVLFITGHQSIKEL 71 RHE S+ A G+ + K GV F TSGPG TNLLT A A+A++ P++ +TG + + Sbjct: 45 RHEQSAAHMADGFGRVSRKPGVCFATSGPGATNLLTGIATAQADSAPMVAVTGQVPVAMI 104 Query: 72 GIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSGQRGPVHLCIPF 131 G Q+S D+ M PA YS + T+ IA +G+ GPV L IP Sbjct: 105 GKDAFQES-----DIIGMANPALKYSYQPRTPEEIPTMVKQGFYIAETGRPGPVLLDIPK 159 Query: 132 DVQTEVLEECRIVIPEREHLV--------SAGNLERVLSAINESSHPLIIAGKGVNRSGA 183 DVQ E I P+ + N R + + + P+I+AG GV S A Sbjct: 160 DVQQN---EANIPFPDEVRVPGYHPWTDPDMQNTARAVELLLSAHKPIILAGGGVIISSA 216 Query: 184 HEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLNRSDVFIVLGSR 243 EL +AE IPVVT+ GKGA DHPL GPIG+ G L+ +D + +G+R Sbjct: 217 FAELQSIAELLMIPVVTTFKGKGAFPEDHPLSLGPIGMHGHAEANKLMTEADCVLAIGTR 276 Query: 244 LSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRDNLAFYLQNFDSAAIK 303 SD ++ + +I DVDP +GK T+ ++AV GD+R +L + + A++ Sbjct: 277 FSDRSVGTFEEFEKNLKIIHMDVDPAEIGKNQTT-SVAVVGDVRTSLRIFGKLLMDKAVR 335 Query: 304 --------KHETETDVHYAEELPVLP-KLSLASVMDALSDLIPYNNTIFVDDGSHGFNAA 354 KH ET ++ E L + P ++ A ++ L +L+P + + + G H A+ Sbjct: 336 PSDDNPWLKHVKETKQYWRENLKLHPGEMGAAKILRKLRELLPKESIVTTEVGQHQMWAS 395 Query: 355 KWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFMMLGTEINTAVC 414 ++ PG+ MG AIGAK A P+ ++ I GDG F M + TAV Sbjct: 396 LFFDAIHPGTFFSSTGLGTMGWGFPAAIGAKTARPDVPVVDIAGDGSFNMTEHSLATAVL 455 Query: 415 KDIPVIFIVVNNMQLDMALKGMEKT--TGRIDGTIFEVPMDAVKFAESLGATGYRCETLE 472 +D+PVI ++NN L M + ++T R+ G + D VK AES GA G R ++++ Sbjct: 456 EDLPVIVFLINNYSLGMVAQ-WQRTFYDRRMVGVDLKKCPDYVKLAESYGAQGIRAQSMD 514 Query: 473 QFTSAIHAAVASNRVAVIELLTDRTE 498 + AI + S+ VI++ D E Sbjct: 515 ELEKAIKDGLNSDVATVIDIPIDPEE 540 >gb|EAI46301.1| unknown [environmental sequence] Length = 559 Score = 241 bits (616), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 165/506 (32%), Positives = 250/506 (49%), Gaps = 29/506 (5%) Query: 12 RHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVLFITGHQSIKEL 71 RHE S+ A G+ + K GV F TSGPG TNLLT A A+A++ P++ +TG + + Sbjct: 45 RHEQSAAHMADGFGRVSRKPGVCFATSGPGATNLLTGIATAQADSAPMVAVTGQVPVAMI 104 Query: 72 GIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSGQRGPVHLCIPF 131 G Q+S D+ M PA YS + T+ IA +G+ GPV L IP Sbjct: 105 GKDAFQES-----DIIGMANPALKYSYQPRTPEEIPTMVKQGFYIAETGRPGPVLLDIPK 159 Query: 132 DVQTEVLEECRIVIPEREHLV--------SAGNLERVLSAINESSHPLIIAGKGVNRSGA 183 DVQ E I P+ + N R + + + P+I+AG GV S A Sbjct: 160 DVQQN---EGNIPFPDEVRVPGYHPWTDPDMQNTGRAVELLLSAKKPIILAGGGVIISSA 216 Query: 184 HEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLNRSDVFIVLGSR 243 EL +AE IPVVT+ GKGA +HPL GPIG+ G L+ +D + +G+R Sbjct: 217 FAELQSIAELLMIPVVTTFKGKGAFPENHPLSLGPIGMHGHAEANKLMTEADCVLAIGTR 276 Query: 244 LSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRDNLAFYLQNFDSAAIK 303 SD ++ + +I DVDP +GK T+ ++AV GD+R +L + + A++ Sbjct: 277 FSDRSVGTFEEFEKNLKIIHMDVDPAEIGKNQTT-SVAVVGDVRTSLRIFGKLLMDKAVR 335 Query: 304 --------KHETETDVHYAEELPVLP-KLSLASVMDALSDLIPYNNTIFVDDGSHGFNAA 354 KH ET ++ E L + P ++ A ++ L +L+P + + + G H A+ Sbjct: 336 ASDDNPWLKHVKETKQYWRENLKIHPGEMGAAKILRKLRELLPKESIVTTEVGQHQMWAS 395 Query: 355 KWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFMMLGTEINTAVC 414 ++ PG+ MG AIGAK A P+ ++ I GDG F M + TAV Sbjct: 396 LFFDAIHPGTFFSSTGLGTMGWGFPAAIGAKTARPDVPVVDIAGDGSFNMTEHSLATAVL 455 Query: 415 KDIPVIFIVVNNMQLDMALKGMEKT--TGRIDGTIFEVPMDAVKFAESLGATGYRCETLE 472 +D+PVI ++NN L M + ++T R+ G + D VK AES GA G R ++++ Sbjct: 456 EDLPVIVFLINNYSLGMVAQ-WQRTFYDRRMVGVDLKKCPDYVKLAESYGAQGIRAQSMD 514 Query: 473 QFTSAIHAAVASNRVAVIELLTDRTE 498 + AI + S+ VI++ D E Sbjct: 515 ELEKAIKDGLNSDVATVIDIPIDPEE 540 >ref|ZP_00077013.1| COG0028: Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase] [Methanosarcina barkeri] Length = 588 Score = 238 bits (608), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 171/513 (33%), Positives = 257/513 (50%), Gaps = 32/513 (6%) Query: 1 MVDYGIEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVL 60 + D + I RHE ++ A GYA TGK GV TSGPG TNL+T A A +++P++ Sbjct: 42 IYDSNLRHILVRHEQAAAHAADGYARATGKTGVCVSTSGPGATNLVTGIATAYMDSVPIV 101 Query: 61 FITGHQSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSG 120 +TG +G Q+ AD+ + +P T ++ LV+ + I A IA +G Sbjct: 102 ALTGQVPTFLIGNDAFQE-----ADIKGITQPITKHNYLVQDANELPRIVKEAFHIASTG 156 Query: 121 QRGPVHLCIPFDVQTEVLEECRIVIPEREHL-----VSAGNLERVLSAINE---SSHPLI 172 + GPV + +P DVQ E P++ L GN +++ A E S P+I Sbjct: 157 RPGPVLIDLPKDVQNA---EIDFYYPDKVELRGYKPTYKGNTQQIKRAAEEIANSCMPII 213 Query: 173 IAGKGVNRSGAHEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLN 232 AG GV S A EL++LAET N PV T+ G GAI +HPL+ G +G+ G K + Sbjct: 214 YAGGGVISSNASAELVELAETINAPVTTTLLGIGAIPTEHPLHVGMLGMHGTKSANYAIQ 273 Query: 233 RSDVFIVLGSRLSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRD---N 289 SD+ I +G+R D L++ +I DVDP + K + + I + GD + + Sbjct: 274 ESDLIIAVGARFDDRVTGKLESFAPNARVIHIDVDPAEISKNVNVQ-IPIVGDAKQVLKS 332 Query: 290 LAFYLQNFDSAA----IKKHETETDVHYAEELP--VLPKLSLASVMDALSDLIPYNNTIF 343 L Y++ S A IK+ + E + Y + ++P+ + + +A D I I Sbjct: 333 LNKYIKCCKSEAWLEKIKQWKKEYPLTYKQMSSDVIMPQFVIEQISEACKDAI-----IV 387 Query: 344 VDDGSHGFNAAKWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFM 403 + G H AA+++K KKP + + MG AIGAKV P++T++ I GDG F Sbjct: 388 TEVGQHQMWAAQFFKYKKPRTFITSGGLGTMGYGFPAAIGAKVGKPDKTVINIAGDGSFQ 447 Query: 404 MLGTEINTAVCKDIPVIFIVVNNMQLDMALKGMEK-TTGRIDGTIFEVPMDAVKFAESLG 462 M E+ T V DIPV+ +++NN L M + E R T + +D VK AE+ G Sbjct: 448 MNSQELATVVQNDIPVVSVIINNGYLGMVRQWQELFYDKRYSQTFIKGSVDFVKLAEAYG 507 Query: 463 ATGYRCETLEQFTSAIHAAVASNRVAVIELLTD 495 A G R E + AI AV S R AV+E++ + Sbjct: 508 ALGLRAERPSEVRPAIEEAVNSGRPAVVEVIVE 540 >ref|NP_618663.1| acetolactate synthase, large subunit [Methanosarcina acetivorans str. C2A] gb|AAM07143.1| acetolactate synthase, large subunit [Methanosarcina acetivorans str. C2A] Length = 564 Score = 235 bits (600), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 167/513 (32%), Positives = 252/513 (49%), Gaps = 32/513 (6%) Query: 1 MVDYGIEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVL 60 + D + I RHE ++ A GYA TGK GV TSGPG TNL+T A A +++P++ Sbjct: 42 LYDSDLRHILVRHEQAAAHAADGYARATGKTGVCVSTSGPGATNLVTGIATAYMDSVPIV 101 Query: 61 FITGHQSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSG 120 +TG +G Q+ AD+ + P T ++ LV+ + I A IA +G Sbjct: 102 ALTGQVPRPLIGNDAFQE-----ADITGITLPITKHNYLVQDAEEIPRIVKEAFHIASTG 156 Query: 121 QRGPVHLCIPFDVQTEVLEECRIVIPEREHL-----VSAGNLERVLSAINE---SSHPLI 172 + GPV + +P DVQ E + P++ L GN +++ A E S P+I Sbjct: 157 RPGPVLIDLPKDVQNI---EIDLQYPDKVELRGYKPTYKGNTQQIKRAAEEIANSCRPII 213 Query: 173 IAGKGVNRSGAHEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLN 232 AG GV S A EL++LAET PV T+ G AI +HPLY G +G+ GCK+ + Sbjct: 214 YAGGGVISSNASAELVELAETLMAPVTTTLMGISAIPTEHPLYVGMLGMHGCKYANYAVQ 273 Query: 233 RSDVFIVLGSRLSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRD---N 289 SD+ I +G+R D L++ +I DVDP + K + + + GD + + Sbjct: 274 ESDLIIAVGARFDDRVTGKLESFAPNARVIHIDVDPAEISKNVKVH-VPIVGDAKQILKS 332 Query: 290 LAFYLQNFDSAAI------KKHETETDVHYAEELPVLPKLSLASVMDALSDLIPYNNTIF 343 L Y+Q +SA K E Y ++P+ + + + D I I Sbjct: 333 LIKYIQRCNSAEWIEKINQWKKEYPLAYRYVSSDTIMPQFVVEQISEVCKDAI-----IV 387 Query: 344 VDDGSHGFNAAKWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFM 403 + G H AA+++K +KP + + MG + A+GAKV P++T++ I GDG F Sbjct: 388 TEVGQHQMWAAQYFKYRKPRTFLTSGGLGTMGYGLPAAMGAKVGKPDKTVINIAGDGSFQ 447 Query: 404 MLGTEINTAVCKDIPVIFIVVNNMQLDMALKGMEK-TTGRIDGTIFEVPMDAVKFAESLG 462 M E+ T V DIPV+ +++NN L M + E R T + +D VK AE+ G Sbjct: 448 MNSQELATLVQNDIPVVSVILNNGYLGMVRQWQELFYDRRYSHTFIKGSVDFVKLAEAYG 507 Query: 463 ATGYRCETLEQFTSAIHAAVASNRVAVIELLTD 495 A G R E + AI AV S R AV+E++ + Sbjct: 508 ALGLRAEKPSEVRPAIEEAVGSGRPAVVEVIVE 540 >ref|NP_632694.1| Acetolactate synthase large subunit [Methanosarcina mazei Goe1] gb|AAM30366.1| Acetolactate synthase large subunit [Methanosarcina mazei Goe1] Length = 564 Score = 228 bits (582), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 167/512 (32%), Positives = 250/512 (48%), Gaps = 31/512 (6%) Query: 1 MVDYGIEFIPGRHESSSGFEAAGYALKTGKLGVAFGTSGPGGTNLLTAAAHAKANNLPVL 60 + D + I RHE ++ A GYA TGK GV TSGPG TNL+T A A +++P++ Sbjct: 42 LYDSDLRHILVRHEQAAAHAADGYARATGKTGVCVSTSGPGATNLVTGIATAYMDSVPIV 101 Query: 61 FITGHQSIKELGIPQCQDSSAYLADLADMFRPATLYSKLVERGDHFGTIFNHAISIALSG 120 +TG +G Q+ AD+ + P T ++ LV+ I A IA +G Sbjct: 102 ALTGQVPRSLIGNDAFQE-----ADITGITMPITKHNYLVQDPQEIPRIVKEAFHIASTG 156 Query: 121 QRGPVHLCIPFDVQTEVLEECRIVIPEREHL-----VSAGNLERVLSAINE---SSHPLI 172 + GPV + +P DVQ E + PER L GN +++ A E S P+I Sbjct: 157 RPGPVLIDLPKDVQNI---EIDLHYPERVELRGYKPTYKGNTQQIKRAAEEIANSCRPII 213 Query: 173 IAGKGVNRSGAHEELLQLAETFNIPVVTSPGGKGAIAWDHPLYHGPIGVGGCKHGEDLLN 232 AG GV S A EL++ AET PV T+ G +I +HPLY G +G+ GCK+ + Sbjct: 214 YAGGGVISSNASAELVEFAETIKAPVTTTLMGISSIPTEHPLYVGMLGMHGCKYANYAIQ 273 Query: 233 RSDVFIVLGSRLSDMTICNLKAENHPKTLIQFDVDPTFVGKILTSETIAVSGDLRD---N 289 SD+ I +G+R D L++ +I DVDP + K + I + GD + + Sbjct: 274 ESDLIIAVGARFDDRVTGKLESFAPNARVIHIDVDPAEISKNVKVH-IPIVGDAKQVLKS 332 Query: 290 LAFYLQNFDSA----AIKKHETETDVHYAE-ELPVLPKLSLASVMDALSDLIPYNNTIFV 344 L Y+Q SA + + E ++Y E ++P+ + + + D I I Sbjct: 333 LIRYVQCCRSAEWIEKVNLWKKEYPLNYRECRDTIMPQFVIEQISEVCRDAI-----IVT 387 Query: 345 DDGSHGFNAAKWYKVKKPGSVVFDAYFACMGNSIGMAIGAKVASPEETILCITGDGCFMM 404 + G H AA+++K KP + + MG A+GAKV P++T++ I GDG F M Sbjct: 388 EVGQHQMWAAQFFKYSKPRTFLTSGGLGTMGYGFPAAMGAKVGRPDKTVINIAGDGSFQM 447 Query: 405 LGTEINTAVCKDIPVIFIVVNNMQLDMALKGMEK-TTGRIDGTIFEVPMDAVKFAESLGA 463 E+ T V DIPV+ +++NN L M + E R T + +D VK AE+ GA Sbjct: 448 NSQELATVVQNDIPVVSVILNNGYLGMVRQWQELFYDRRYSYTFIKGSVDFVKLAEAYGA 507 Query: 464 TGYRCETLEQFTSAIHAAVASNRVAVIELLTD 495 G R E + AI AV S R V+E++ + Sbjct: 508 LGLRAERPSEVRPAIEEAVNSGRPTVVEVIVE 539 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 765,141,994 Number of extensions: 32141490 Number of successful extensions: 83028 Number of sequences better than 1.0e-05: 2448 Number of HSP's gapped: 79527 Number of HSP's successfully gapped: 2573 Length of query: 510 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 380 Effective length of database: 404,274,708 Effective search space: 153624389040 Effective search space used: 153624389040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)