BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0244.0005.p (209 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_981364.1| O-methyltransferase [Bacillus cereus ATCC 10987... 195 6e-49 ref|NP_978360.1| O-methyltransferase, putative [Bacillus cereus ... 169 3e-41 ref|NP_691216.1| O-methyltransferase [Oceanobacillus iheyensis H... 163 3e-39 ref|NP_242694.1| O-methyltransferase [Bacillus halodurans] >gi|2... 159 6e-38 ref|NP_899863.1| O-methyltransferase [Chromobacterium violaceum ... 149 5e-35 >ref|NP_981364.1| O-methyltransferase [Bacillus cereus ATCC 10987] gb|AAS43972.1| O-methyltransferase [Bacillus cereus ATCC 10987] Length = 212 Score = 195 bits (495), Expect = 6e-49, Method: Compositional matrix adjust. Identities = 101/211 (47%), Positives = 139/211 (65%), Gaps = 2/211 (0%) Query: 1 MPNQEEYSEQLY-TEDALLLKVKQAIKDSGMPEVSIAPGYGRLLTMLVAASRSTRILEIG 59 M Y + ++ ++D LL +V +IK++ MP +S++P G+LLTMLV+ S + ILEIG Sbjct: 1 MKQINRYIDSVFCSQDTLLDEVIASIKENEMPPISVSPSSGKLLTMLVSISGAKDILEIG 60 Query: 60 ALGGYSGICLCRGLSKAGRLTSLELKAEYAELAGRHLEEAGFGEAVEYKLGPALDSLKLL 119 ALGGYSGICL +G + G LTSLEL+ +YA++A +L +AGFG+ V Y G AL SL++L Sbjct: 61 ALGGYSGICLAKGFGEGGTLTSLELEEKYAKVAHSNLHKAGFGKQVSYMTGAALQSLEIL 120 Query: 120 EAEGRTFDFFFIDADKENYPNYLEYAIKLAAPGAVIAGDNIFLRGRTLN-AEKNGPAVQA 178 FDFFFIDADKENY NYL Y IKLA PGA+I DN+ G + K + Sbjct: 121 ATGNNRFDFFFIDADKENYENYLTYCIKLAKPGALIVTDNVLAGGSVADEGAKQKRYTEV 180 Query: 179 MRRFNEMIANDERLTSTLLPAYDGLALAVVK 209 M++FNE++A L S L+P DG+ ++ VK Sbjct: 181 MKKFNEVVAAHPNLESVLIPIGDGMTVSKVK 211 >ref|NP_978360.1| O-methyltransferase, putative [Bacillus cereus ATCC 10987] gb|AAS40968.1| O-methyltransferase, putative [Bacillus cereus ATCC 10987] Length = 223 Score = 169 bits (429), Expect = 3e-41, Method: Compositional matrix adjust. Identities = 90/208 (43%), Positives = 127/208 (61%), Gaps = 6/208 (2%) Query: 8 SEQLYTEDALLLKVKQAIKDSGMPEVSIAPGYGRLLTMLVAASRSTRILEIGALGGYSGI 67 S+ L +D+ L +V Q + +P ++P G+ L +LV + ILEIG LGGYS I Sbjct: 15 SDVLIPKDSTLEEVLQVNAAANLPAHDVSPTQGKFLQLLVQIQGARNILEIGTLGGYSTI 74 Query: 68 CLCRGLSKAGRLTSLELKAEYAELAGRHLEEAGFGEAVEYKLGPALDSLKLLEAEG-RTF 126 L RGLS GR+ +LE ++A++A ++E A + VE + G ALDSL+ +E E F Sbjct: 75 WLARGLSSGGRVVTLEASEKHADIARSNIERANLNDRVEVRTGLALDSLQQIENEKYEPF 134 Query: 127 DFFFIDADKENYPNYLEYAIKLAAPGAVIAGDNIFLRGRTLNAEKNGPAVQAMRRFNEMI 186 DF FIDADK+N P Y E+A+KL+ PG VI GDN+ G ++ N P VQ +RRF E+I Sbjct: 135 DFIFIDADKQNNPAYFEWALKLSRPGTVIIGDNVVREGEVIDNTSNDPRVQGIRRFYELI 194 Query: 187 ANDERLTSTLLPA-----YDGLALAVVK 209 A + R+++T L YDG +AVVK Sbjct: 195 AAEPRVSATALQTVGSKGYDGFIMAVVK 222 >ref|NP_691216.1| O-methyltransferase [Oceanobacillus iheyensis HTE831] dbj|BAC12251.1| O-methyltransferase [Oceanobacillus iheyensis HTE831] Length = 221 Score = 163 bits (412), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 80/213 (37%), Positives = 130/213 (61%), Gaps = 6/213 (2%) Query: 3 NQEEYSE-QLYTEDALLLKVKQAIKDSGMPEVSIAPGYGRLLTMLVAASRSTRILEIGAL 61 N +EY E +L +D +L + + +G+P++ +AP G+LL +L + ++LEIG L Sbjct: 9 NVDEYFEAKLLPDDKILNQTLDNNQAAGLPQIDVAPLQGKLLYLLTKMKSARQVLEIGTL 68 Query: 62 GGYSGICLCRGLSKAGRLTSLELKAEYAELAGRHLEEAGFGEAVEYKLGPALDSLKLLEA 121 GGYS I + +++ G++ SLE +AE+A +++E AGF + V+ +G ALD+L LE Sbjct: 69 GGYSSIWFAKAVTQNGKVISLEANQSHAEVARQNIENAGFKQTVQVLVGNALDTLPTLEN 128 Query: 122 EGRTFDFFFIDADKENYPNYLEYAIKLAAPGAVIAGDNIFLRGRTLNAEKNGPAVQAMRR 181 G +FD FIDADK+N P YLE+A++LA GA++ DN+ G ++ + +Q +R Sbjct: 129 AGESFDVIFIDADKQNNPKYLEWALRLANEGALLIADNVVRNGEVIDQKSEDERIQGLRE 188 Query: 182 FNEMIANDERLTSTLLPA-----YDGLALAVVK 209 F M+ N+ER+ ST + YDG + +VK Sbjct: 189 FVNMLENEERIESTAIQTVGSKDYDGFVIGIVK 221 >ref|NP_242694.1| O-methyltransferase [Bacillus halodurans] pir||D83878 O-methyltransferase mdmC [imported] - Bacillus halodurans (strain C-125) dbj|BAB05547.1| O-methyltransferase [Bacillus halodurans] Length = 223 Score = 159 bits (401), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 79/209 (37%), Positives = 127/209 (60%), Gaps = 6/209 (2%) Query: 7 YSEQLYTEDALLLKVKQAIKDSGMPEVSIAPGYGRLLTMLVAASRSTRILEIGALGGYSG 66 +SE+L+ D ++ V +A + + +P + +AP G+ L +L ILEIG LGGYS Sbjct: 14 FSEKLHAFDPVMDWVLEANEKAELPPIDVAPNQGKFLYLLAKLKGVKTILEIGTLGGYSS 73 Query: 67 ICLCRGLSKAGRLTSLELKAEYAELAGRHLEEAGFGEAVEYKLGPALDSLKLLEAEG-RT 125 I + R L GRL +LE ++A +A ++++AG ++ +GPAL++L L+ +G + Sbjct: 74 IWMARALPDDGRLVTLEFSEKHAAIAQSNMKKAGVDGKIDVIIGPALETLPTLKDKGFSS 133 Query: 126 FDFFFIDADKENYPNYLEYAIKLAAPGAVIAGDNIFLRGRTLNAEKNGPAVQAMRRFNEM 185 FD FIDADK N P+YLE+A+KL+ PG +I GDN+ G LN E P+++ +R+F + Sbjct: 134 FDMIFIDADKPNNPHYLEWALKLSTPGTLIVGDNVVRNGNVLNKESADPSIKGIRQFIHL 193 Query: 186 IANDERLTSTLLPA-----YDGLALAVVK 209 ++ + R+ ST + YDG + VVK Sbjct: 194 LSEEPRIESTAIQTVGIKGYDGFVVGVVK 222 >ref|NP_899863.1| O-methyltransferase [Chromobacterium violaceum ATCC 12472] gb|AAQ57872.1| O-methyltransferase [Chromobacterium violaceum ATCC 12472] Length = 221 Score = 149 bits (375), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 86/209 (41%), Positives = 120/209 (57%), Gaps = 6/209 (2%) Query: 7 YSEQLYTEDALLLKVKQAIKDSGMPEVSIAPGYGRLLTMLVAASRSTRILEIGALGGYSG 66 + QL +DA L +G+P +S+AP G+ L +L + RILEIG LGGYS Sbjct: 12 FCRQLVHQDAALEAALADSAAAGLPAISVAPNQGKFLNLLARIHGARRILEIGTLGGYSA 71 Query: 67 ICLCRGLSKAGRLTSLELKAEYAELAGRHLEEAGFGEAVEYKLGPALDSLKLLEAEGRT- 125 I L R L + GRL +LE ++ + E+A +L AG V+ G A DSL+ L +G Sbjct: 72 IWLARALPENGRLVTLEYESRHVEVAAANLARAGVAGKVDIIQGAAADSLQRLIDDGAAP 131 Query: 126 FDFFFIDADKENYPNYLEYAIKLAAPGAVIAGDNIFLRGRTLNAEKNGPAVQAMRRFNEM 185 FD FIDADK N P YLE A++L+ PG VI GDN+ +G N + PA+ RRF E+ Sbjct: 132 FDLVFIDADKPNNPVYLELALRLSRPGTVIVGDNVARQGEVANPDNPDPAIVGTRRFIEL 191 Query: 186 IANDERLTSTLL-----PAYDGLALAVVK 209 + + RL++T + YDG ALA+V+ Sbjct: 192 LGSHPRLSATAIQTVGGKGYDGFALAIVE 220 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 296,185,273 Number of extensions: 11804227 Number of successful extensions: 30589 Number of sequences better than 1.0e-05: 415 Number of HSP's gapped: 30275 Number of HSP's successfully gapped: 415 Length of query: 209 Length of database: 757,237,578 Length adjustment: 120 Effective length of query: 89 Effective length of database: 431,425,698 Effective search space: 38396887122 Effective search space used: 38396887122 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 123 (52.0 bits)