BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0243.0009.p (330 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella th... 75 2e-12 ref|NP_624131.1| Subtilisin-like serine proteases [Thermoanaerob... 70 7e-11 ref|ZP_00061549.1| COG2730: Endoglucanase [Clostridium thermocel... 63 8e-09 ref|ZP_00060097.1| hypothetical protein [Clostridium thermocellu... 61 4e-08 dbj|BAC45001.1| xylanase 5 [Paenibacillus sp. W-61] 60 5e-08 >ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella thermoacetica ATCC 39073] Length = 368 Score = 75.5 bits (184), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 56/171 (32%), Positives = 74/171 (43%), Gaps = 14/171 (8%) Query: 41 AESKINALHNKGVVNGVTTDRFAPKSKVTFAQGIQFIVNGLQLSPKAXXXXXXXXXXXYF 100 A+ I L G+V GV F P VT A+ +V L L KA F Sbjct: 203 AQDTIETLAGAGIVAGVDGSHFEPGRAVTRAEFASLLVKALGLEGKAGEKNP-------F 255 Query: 101 DKVKDKAWYASAFLIAKQNGLTL---DKSVDPNAAMTRIQFAHLLTQALQSKGNFPVTLM 157 VK AWYA A A NGL D + P+ +TR + A +L +A+ N P Sbjct: 256 KDVKGDAWYAGAVTAAASNGLVKGYEDGTFRPDNVITREELAAMLVRAV----NLPAGEE 311 Query: 158 YADISDGSKLSNAEMNSLQILVNTHLVTLEKNNTFRPNDAVTRAEAAVWIY 208 D K+S S+ + LV ++ TFRP DA +RAE AV +Y Sbjct: 312 KLAFKDNDKVSTWARKSVAVAAAHGLVKGFEDGTFRPGDAASRAECAVMVY 362 >ref|NP_624131.1| Subtilisin-like serine proteases [Thermoanaerobacter tengcongensis] gb|AAM25735.1| Subtilisin-like serine proteases [Thermoanaerobacter tengcongensis] Length = 1999 Score = 70.1 bits (170), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 48/171 (28%), Positives = 81/171 (47%), Gaps = 11/171 (6%) Query: 45 INALHNKGVVNGVTTDRFAPKSKVTFAQGIQFIVNGLQLSPKAXXXXXXXXXXXYFDKVK 104 IN L ++ ++ G+T D FAP +K+T A+ ++ L + + F+ VK Sbjct: 1832 INVLASRHIIKGMTEDTFAPNAKITRAEFAALMIRALGIPEEPYKGE--------FEDVK 1883 Query: 105 DKAWYASAFLIAKQNGLTLD--KSVDPNAAMTRIQFAHLLTQALQSKGNF-PVTLMYADI 161 + WYA+A A Q G+ L K + P+ +TR + A ++ + + + Sbjct: 1884 EGMWYANAIEAAYQEGIMLGDGKKMRPDDPITREEMAAVIMRVYSKLAGYKEENIGNTTF 1943 Query: 162 SDGSKLSNAEMNSLQILVNTHLVTLEKNNTFRPNDAVTRAEAAVWIYDAAE 212 +D +K+S N + V +V +NTF+P D TRAEAA +Y E Sbjct: 1944 ADNNKISEWARNVVANAVKLGIVRGYDDNTFKPKDNATRAEAAAMLYRILE 1994 >ref|ZP_00061549.1| COG2730: Endoglucanase [Clostridium thermocellum ATCC 27405] Length = 1013 Score = 63.2 bits (152), Expect = 8e-09, Method: Compositional matrix adjust. Identities = 49/176 (27%), Positives = 79/176 (44%), Gaps = 13/176 (7%) Query: 37 KGDPAESKINALHNKGVVNGVTTDRFAPKSKVTFAQGIQFIVNGLQLSPKAXXXXXXXXX 96 +G AE I L+ KG+++G+ F P +T A+ Q IVN L++ + Sbjct: 840 RGYWAEEYIQKLYEKGIISGIDEKTFMPGRPITRAEFTQIIVNSLKIPYRE--------A 891 Query: 97 XXYFDKVKDKAWYASAFLIAKQNGLTL---DKSVDPNAAMTRIQFAHLLTQALQSK--GN 151 +F+ V +K WY + A G+ + D S PN +TR A ++ + L+ K GN Sbjct: 892 GLHFNDVTEKDWYYKSVSSAAAFGIVVGRPDGSFAPNEFITRQDMAVVIAKFLEKKHDGN 951 Query: 152 FPVTLMYADISDGSKLSNAEMNSLQILVNTHLVTLEKNNTFRPNDAVTRAEAAVWI 207 +D +S +S+ +V+ L+ + N F P TRAEA I Sbjct: 952 LEGMGKGLVFADSGNISEYARDSVAAVVSQGLMVGKPGNMFDPKGLTTRAEAVTVI 1007 >ref|ZP_00060097.1| hypothetical protein [Clostridium thermocellum ATCC 27405] pir||JC6031 scaffoldin dockerin binding protein precursor - Clostridium thermocellum gb|AAB07763.1| SdbA Length = 631 Score = 60.8 bits (146), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 49/174 (28%), Positives = 80/174 (45%), Gaps = 16/174 (9%) Query: 41 AESKINALHNKGVVNGVTTDRFAPKSKVTFAQGIQFIVNGLQLSPKAXXXXXXXXXXXYF 100 A+ +I L +KGV+NG + F P++ +T A + +V L L+ + F Sbjct: 463 AKKQIEVLASKGVINGTSDTTFTPQADITRADFMILLVKALGLTAEVTSN---------F 513 Query: 101 DKVKDKAWYASAFLIAKQNGLTL---DKSVDPNAAMTRIQFAHLLTQALQSKGNFPVTLM 157 D V +K +Y IAK+ G+T + +P A +TR L T AL+ G T Sbjct: 514 DDVSEKDYYYEYVGIAKELGITTGVGNNKFNPKAKITRQDMMVLTTNALRIAGKISSTGT 573 Query: 158 YADI---SDGSKLSNAEMNSLQILVNTHLVTLEKNNTFRPNDAVTRAEAAVWIY 208 AD+ SD ++++ + + LV +V + + P +RAE A IY Sbjct: 574 RADVERFSDKDQIASYAVEGVATLVKEGIV-VGSGDIINPRGNASRAELAAIIY 626 >dbj|BAC45001.1| xylanase 5 [Paenibacillus sp. W-61] Length = 1326 Score = 60.5 bits (145), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 50/170 (29%), Positives = 84/170 (49%), Gaps = 14/170 (8%) Query: 41 AESKINALHNKGVVNGVTTDRFAPKSKVTFAQGIQFIVNGLQLSPKAXXXXXXXXXXXYF 100 A+ I A+ + ++ G++ + FAP + +T A I +V L+L K F Sbjct: 1158 AKEAIEAMAARDIIKGISDESFAPAASITRADFITLLVRALELKGKDTNAAA-------F 1210 Query: 101 DKVKDKAWYASAFLIAKQNGLTL---DKSVDPNAAMTRIQFAHLLTQALQSKGNFPVTLM 157 V+ A+YA A IAK+ G+ D + P ++++R L +AL++ G P T Sbjct: 1211 SDVQSTAYYAQAVAIAKELGIASGFEDNTFKPGSSISRQDMMVLTAKALKAAGK-PSTGS 1269 Query: 158 --YADISDGSKLSNAEMNSLQILVNTHLVTLEKNNTFRPNDAVTRAEAAV 205 A SD + +S ++S+ LV + +V K P +++TRAEAAV Sbjct: 1270 GNLAPYSDAASISTYAVDSVTSLVGSGIVN-GKGGKIAPTESLTRAEAAV 1318 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.131 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 421,655,860 Number of extensions: 15986332 Number of successful extensions: 35193 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 35190 Number of HSP's successfully gapped: 17 Length of query: 330 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 204 Effective length of database: 415,135,104 Effective search space: 84687561216 Effective search space used: 84687561216 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)