BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0243.0006.p (348 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_349437.1| Uncharacterized conserved protein, YAEG family ... 174 3e-42 ref|NP_816283.1| conserved domain protein [Enterococcus faecalis... 150 6e-35 ref|ZP_00087961.1| COG3835: Sugar diacid utilization regulator [... 125 2e-27 ref|NP_693706.1| hypothetical protein OB2784 [Oceanobacillus ihe... 116 7e-25 ref|ZP_00090346.1| COG3835: Sugar diacid utilization regulator [... 111 3e-23 >ref|NP_349437.1| Uncharacterized conserved protein, YAEG family [Clostridium acetobutylicum] pir||F97248 uncharacterized conserved protein, YAEG family CAC2833 [imported] - Clostridium acetobutylicum gb|AAK80777.1| Uncharacterized conserved protein, YAEG family [Clostridium acetobutylicum] Length = 344 Score = 174 bits (441), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 106/340 (31%), Positives = 179/340 (52%), Gaps = 5/340 (1%) Query: 10 QLSENQAQEIVDKMMTDIPYNINIMNADXXXXXXXXXXXXXTVHQGAVQALSTGRMVEVW 69 +LS+ AQ IV++MM IPYNIN+MN + +H+GA++A+ + ++ Sbjct: 2 RLSKTIAQSIVEEMMNLIPYNINVMNEEGIIIGSGDLSRIGNLHEGALEAIKEKSLNVIY 61 Query: 70 EDSRFEKKGTNEPIVIGHTRVGVIGISGNPDEVRPFCNIVRTTVSLLIEQRNSLETLAHE 129 + K G NEPI+I +GVIGI+G DEV+ F +VR T LLIEQ + E + Sbjct: 62 SEKNSVKPGVNEPIIIDGKVIGVIGITGYSDEVKKFIKLVRVTAVLLIEQAEANEESQNR 121 Query: 130 ANRKKAFLELLLNHQGAYSQKLRKEAAPYQIDLLLKTVVLYIKHFNAEADPDKLLLRYP- 188 +++ F L+N + Y +K K A + D+ K + + N L +Y Sbjct: 122 NLKRERFYYDLINRKTPYDEKFSKIAQDFGFDISKKHRAILVYGNNTSKKFYDLKKKYIF 181 Query: 189 SFAIDEETQLLILQESGDPGPLIRELLRGQPQVLIAAGKQEASIAESYRQARSAMNILLA 248 ++ +D + + LI EL + I G++E A S ++ + + Sbjct: 182 NYDLDINKTAFFISDDYKYKLLIDELKHYKDIEKIGIGEKEDIAAISLENSKKGVELGKK 241 Query: 249 LKPAAQVIAFENVEFLVKLSHAS----LAPLNTAAKLEDAVDMIETLRSFINHNCSVSHT 304 +KP +++ +E ++F + L H + + +++ K E +++I+T++ ++ N ++ Sbjct: 242 IKPNSKIYEYEELKFFIHLHHKNKEEIVNLISSIDKSEYKLELIQTIQIYVEENGQINDA 301 Query: 305 ADDLNIHRNTLQYRLKRIQALTGKDPRNLLQLFELTHGLL 344 A LNIHRNTL YRL+RI+ +TGKDP+N QLFEL GL+ Sbjct: 302 ALRLNIHRNTLNYRLERIKKITGKDPKNFFQLFELFCGLI 341 >ref|NP_816283.1| conserved domain protein [Enterococcus faecalis V583] gb|AAO82353.1| conserved domain protein [Enterococcus faecalis V583] Length = 337 Score = 150 bits (378), Expect = 6e-35, Method: Compositional matrix adjust. Identities = 109/349 (31%), Positives = 185/349 (53%), Gaps = 25/349 (7%) Query: 8 LFQLSENQAQEIVDKMMTDIPYNINIMNADXXXXXXXXXXXXXTVHQGAVQALSTGRMVE 67 + +L + QA +IV+K+M DIPYNINIM+ H+GA +A++ + +E Sbjct: 1 MLKLDKIQANKIVEKLMADIPYNINIMDERGKIIASGDSARIGERHRGAERAINERKNIE 60 Query: 68 VWEDSRFEKKGTNEPIVIGHTRVGVIGISGNPDEVRPFCNIVRTTVSLLIEQRNSLETLA 127 +++D+ EKKGTNEPI++ + +GV+GISG PDEVR F +VR+ V LL E+ N+ + Sbjct: 61 IYKDTSLEKKGTNEPIILNNHILGVVGISGEPDEVRKFTKLVRSIVLLLTEELNTQQERE 120 Query: 128 HEANRKKAFLELLLNHQGAYSQKLRKEAAP-YQIDLLLKTVVLYIKHFNAEADPDKLLLR 186 + +K F++ L++ +YS+ L+ EA Y + L + + K DK LL Sbjct: 121 KKKKQKNQFIQHLMHVDSSYSEALKLEALELYNLQLDDQNHCVLTK--------DKRLLS 172 Query: 187 --YPSFAIDEETQLLILQESGDPGPLIRELLRGQPQVLIAAGKQEASIAESYRQARSAMN 244 YP I E ++ S P ++ + + L+ + ++A+I + ++A++ Sbjct: 173 SYYPGHEIFEWENCYLVITSEPP-----QITKPVTEYLVISS-EKATIQQMVQEAQNTY- 225 Query: 245 ILLALKPAAQVIAFENVEFLVK-----LSHASLAPLNTAAKLEDAVDMIETLRSFINHNC 299 +LL+ + ++ +F ++ H L T + D + ET+ F NHN Sbjct: 226 LLLSFLQLEKAKFYQAEDFFIQGIFNFPFHIELDFFETIQTIYD--EYYETVIFFANHNL 283 Query: 300 SVSHTADDLNIHRNTLQYRLKRIQALTGKDPRNLLQLFELTHGLLALYK 348 +++ T+ L+IHRNTL YRLKRI LTG DP+ ++L + +K Sbjct: 284 NINETSQVLHIHRNTLNYRLKRIHELTGMDPKVWQDFWKLFYYFAYCFK 332 >ref|ZP_00087961.1| COG3835: Sugar diacid utilization regulator [Pseudomonas fluorescens PfO-1] Length = 390 Score = 125 bits (313), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 105/369 (28%), Positives = 163/369 (44%), Gaps = 38/369 (10%) Query: 8 LFQLSENQAQEIVDKMMTDIPYNINIMNADXXXXXXXXXXXXXTVHQGAVQALSTGRMVE 67 +F+L + AQ+IVD+ M +PYN+N+M++ T H+GA L+ GR+VE Sbjct: 17 MFELDHDLAQDIVDRAMAILPYNVNVMDSQGLILGSGEPERINTRHEGAQLVLANGRVVE 76 Query: 68 VWEDSRFEKKGT----NEPIVIGHTRVGVIGISGNPDEVRPFCNIVRTTVSLLIEQRNSL 123 + + KG N P+++ +GV+GI+G P+++R + +VR T +L+ QRN Sbjct: 77 IDAQTAVHLKGVQPGINLPLLLDQRLIGVLGITGEPEQLRTYAELVRMTAEMLVGQRNQQ 136 Query: 124 ETLAHEANRKKAFLELLLNHQGAYSQKLRKEAAPYQIDLLLKTVVLYIKHFNAEADPDK- 182 R L LLL+ G S +L EA + L T V Y+ E P + Sbjct: 137 SEQQWRRQRCDDLLALLLSEAGD-SPRLVDEAQQLGLKPQL-TRVPYLFELGLEHGPGQT 194 Query: 183 -------LLLRYP-SFAIDEETQLLILQESGDPGPLIRELLR-----GQPQVLIAAGKQE 229 L+ RYP S+ + L+ LL G + IA G Q Sbjct: 195 VEALSAWLMSRYPDSWCVSSAKSSLLWCRPASQNVEHDRLLEKLDGLGWNILRIAVGGQA 254 Query: 230 ASIAESYRQARSAMNILLALK---PAAQVIAFENVEFLVKLSHAS--------LAPLNTA 278 + R R ++L + P ++++ V L L PL Sbjct: 255 DGLTGLRRCYRRVGDLLAYGREVLPRSRLLTLNRYRLPVMLWRHRNDDALDELLKPLRKV 314 Query: 279 AKLEDAVDMIETLRSFINHNCSVSHTADDLNIHRNTLQYRLKRIQALTGKDPRNLLQLFE 338 + ++ TLRS+ +H+ AD L IHRN+L+YR++RI L+G DP L Sbjct: 315 IAKDSNGQLLATLRSWCDHDGQSQACADALGIHRNSLRYRMERIAELSGVDPLKL----- 369 Query: 339 LTHGLLALY 347 G+LALY Sbjct: 370 --DGMLALY 376 >ref|NP_693706.1| hypothetical protein OB2784 [Oceanobacillus iheyensis HTE831] dbj|BAC14740.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 377 Score = 116 bits (291), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 107/367 (29%), Positives = 172/367 (46%), Gaps = 43/367 (11%) Query: 11 LSENQAQEIVDKMMTDIPYNINIMNADXXXXXXXXXXXXXTVHQGAVQALSTGRMVEV-- 68 L+E A +IV + M+ I NINIM+ +H GAVQ + R +E+ Sbjct: 4 LTEQLASQIVQRTMSIIHANINIMDRTGTIIASGNPSRIGQLHDGAVQVIKEKRTIEIDT 63 Query: 69 -----WEDSRFEKKGTNEPIVIGHTRVGVIGISGNPDEVRPFCNIVRTTVSLLIEQRN-S 122 W+ S + G N PI + +GVIGI+G PD++R + +++ L +EQ + Sbjct: 64 SGATHWQSS---QSGINLPITFQNNIIGVIGITGPPDKIRDYSQLIQMGAELTLEQAFLT 120 Query: 123 LETLAHEANRKKAFLELLLNHQGAYSQKLRKEAAPY-QIDL-------LLKTVVLYIKHF 174 E ++ R+ +LL + Q +E A Y ++D+ ++ T VL KH Sbjct: 121 NEIQQNKQIRQNQITNILLGTE--QDQTYIQERAHYAKLDIQTTYAVIIIFTPVLDKKHT 178 Query: 175 NAEADPDKLLLRYPSFAIDEET--QLLILQESGDPGPL-IRELLRGQ------PQVLIAA 225 + L + I T L++ +E G P I+++L + P + IA Sbjct: 179 EEVEKHIQSWLNHEDEHIRLFTNQHLILKKEIGYSNPAHIKDILVEKHKNSSIPNMTIAV 238 Query: 226 GKQEASI---AESYRQARSAMNILLALKPAAQVIAFENVEF------LVKLSHASLAPLN 276 G + +SY+QA+ + + L P+ V +E++ L+K S L Sbjct: 239 GSYLTGVDGWRQSYQQAQKIIEVTKTLYPSGGVWNYEDLSLEILAYDLLKNKPQSAKELT 298 Query: 277 TAAKL----EDAVDMIETLRSFINHNCSVSHTADDLNIHRNTLQYRLKRIQALTGKDPRN 332 T + D + + +TL+++I N VS T+D L IHRNTL YRL +I +TGKDPRN Sbjct: 299 TTYQRILTESDGIQLHQTLQTYIEENGKVSITSDKLFIHRNTLTYRLDKIHQITGKDPRN 358 Query: 333 LLQLFEL 339 + L EL Sbjct: 359 INHLLEL 365 >ref|ZP_00090346.1| COG3835: Sugar diacid utilization regulator [Azotobacter vinelandii] Length = 367 Score = 111 bits (277), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 95/351 (27%), Positives = 155/351 (44%), Gaps = 31/351 (8%) Query: 8 LFQLSENQAQEIVDKMMTDIPYNINIMNADXXXXXXXXXXXXXTVHQGAVQALSTGRMVE 67 +F+L AQ+IV + M +PYN+N+M++ T H+GA LS R+V Sbjct: 1 MFELDRELAQDIVTRAMAILPYNVNVMDSQGLILASGEPSRIDTRHEGAQLVLSNARVVA 60 Query: 68 VWEDSRFE------KKGTNEPIVIGHTRVGVIGISGNPDEVRPFCNIVRTTVSLLIEQRN 121 + DS+ + G N P+ +GV+GI+G P++VR + ++R T +L+ QR+ Sbjct: 61 I--DSQAAACLKGVQPGINLPLFFDRRLIGVLGITGEPEQVRLYGELLRMTAEMLVAQRH 118 Query: 122 SLETLAHEANRKKAFLELLLNHQGAYSQKLRKEAAPYQID-LLLKTVVLY--IKHFNAEA 178 R + LLL +G + +L EA + L++T L+ +AE Sbjct: 119 QQAERQWRRQRSDDLMALLLE-EGGDAPRLLDEARLLGLKPQLMRTPWLFELAGGQSAER 177 Query: 179 DPDKLLLRYP-SFAIDEETQLLILQESG----DPGP---LIRELLRGQPQVLIAAGKQEA 230 D L RYP S+ + L+ + G +P L R +G + +A G+ Sbjct: 178 LGDWLQGRYPDSWCVSPAAGSLLWCQPGASLDEPAQAQLLERVEAQGWQVLRVAVGELAE 237 Query: 231 SIA---ESYRQARSAMNILLALKPAAQVIAFENVEFLVKLSHAS--------LAPLNTAA 279 + YR+ R + A+ P +++ L H L PL Sbjct: 238 GVEGMRHGYRRVRDLLAYGRAILPRQRLLGLARYRLPTLLWHYRDDEAVRELLRPLQRLR 297 Query: 280 KLEDAVDMIETLRSFINHNCSVSHTADDLNIHRNTLQYRLKRIQALTGKDP 330 + ++ TLR + H AD L +HRN+L+YRL+RI L+G DP Sbjct: 298 ARDAGGQLLGTLRCWCEHRGHGQACADALGVHRNSLRYRLERIAELSGVDP 348 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 443,191,298 Number of extensions: 16585671 Number of successful extensions: 42849 Number of sequences better than 1.0e-05: 47 Number of HSP's gapped: 42784 Number of HSP's successfully gapped: 50 Length of query: 348 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 222 Effective length of database: 415,135,104 Effective search space: 92159993088 Effective search space used: 92159993088 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)