BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0238.0004.p (608 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAB40444.2| alpha-D-mannosidase [Bacillus sp. GL1] 380 e-104 ref|NP_562996.1| alpha-mannosidase [Clostridium perfringens] >gi... 375 e-102 ref|NP_923026.1| putative alpha-mannosidase [Gloeobacter violace... 355 1e-96 ref|NP_465539.1| similar to alpha-mannosidase [Listeria monocyto... 326 1e-87 ref|NP_471457.1| similar to alpha-mannosidase [Listeria innocua]... 325 2e-87 >dbj|BAB40444.2| alpha-D-mannosidase [Bacillus sp. GL1] Length = 1047 Score = 380 bits (976), Expect = e-104, Method: Compositional matrix adjust. Identities = 236/613 (38%), Positives = 323/613 (52%), Gaps = 70/613 (11%) Query: 10 GKQSELKNACNESGGGQPQ-LPEWRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELW 68 G+ + + +E+ G + + W G+LYLE HRGTYT+ NKR NRK E++YREAE Sbjct: 489 GRAGDFFDKLHETVAGTDRYVHTWDGELYLEYHRGTYTSQAYNKRMNRKLELMYREAEWL 548 Query: 69 QTLAGSSIAPELQRSIAGQLHKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGE 128 +L + + L++GW +L NQFHDIIPGS+I Y+ + EY E LG+ Sbjct: 549 HSLGAVAGGGDWSGYPKEALYEGWTTILRNQFHDIIPGSSIGAVYQDARAEYAEAERLGK 608 Query: 129 SSLQPGLEALTAQVAAAGEGIPYVLFNSLGWERDAVV--PVHLPEGRTWAAYDEAGSPLH 186 ++Q E L Q EG ++N ++R +V PV G EAG PL Sbjct: 609 EAIQAAAERLAGQ----AEGAVVSVYNGAAFDRTELVEIPVQAGIGSQGGWLGEAGRPLR 664 Query: 187 --------LDLAADLSARDXXXXXXXXXXXXXXXXXSIERATEQATGQVTAYIHVPSIPG 238 L LA D+ A + + GQ T S+ Sbjct: 665 AEFDGSRWLVLAEDVPATGAATLRF-----------------DPSAGQQT------SVKA 701 Query: 239 FGYKTVWLRQVDEAVTGSMPERRELNDSWETPHYRLAFNERGEITSLFDKSAGREVVQPG 298 F + LR TPHY LAF+E G + L+D+ REV+ G Sbjct: 702 FEWADGVLR---------------------TPHYELAFDEAGRLVRLYDRGLSREVLPRG 740 Query: 299 GRANRFHFFHDRPTLWDAWDIDSRYEAQPAGEAELVEKYVLHSGKVQDVLRFRWKLGQSE 358 RAN F D+P +++AWDID Y+ + +L + ++ G ++ VLR W G S Sbjct: 741 ARANELQVFEDKPKMFEAWDIDLFYQEKRREVTDLRKLELVECGALRAVLRLEWAYGASV 800 Query: 359 ITQDLILYAHDRRIDFKTHVDWNEAHKLLKVGFPVDVVADKATYEIPFGALERPTHRNTS 418 I Q L++YA DRRIDF T VDW E +LLKV FPV++ A +ATY+I FG ++RPTH NTS Sbjct: 801 IEQRLVVYADDRRIDFVTRVDWREHQQLLKVAFPVEIRATEATYDIQFGNVKRPTHWNTS 860 Query: 419 WEQAQYEVCGHRFADVSEHGYGVSLLNDCKYGYDIQGSTIRLSLLRAPKWPDKVADQGAH 478 W+ A++E GH++ D+SE GYGVSLLNDCKYG+DI+ + IRLSL+++ PD ADQG H Sbjct: 861 WDHARFETVGHQWVDLSERGYGVSLLNDCKYGHDIKDNVIRLSLIKSAIDPDPNADQGEH 920 Query: 479 DFTYALYPHTGDWRSAHTLRKAAELNHEVPAIAADGKTTGQLPSTGSFIRFDGQHVVLDT 538 FTY+LYPH GDW S T A LN+ + A+ G+ S +R DG H+ D Sbjct: 921 LFTYSLYPHAGDWASGGTATAAWALNNPLLAVPGASAFAGK-----SLLRADGAHIAFDA 975 Query: 539 VKPAEDGRGSVLRLYESSGGRETVKLAWNQPFSEIYISNALEAELEPV-IHHEGEFELNF 597 +K AED V+RL+E +GGR L P EI + P+ H+G+ L F Sbjct: 976 IKKAEDEDALVVRLHEYAGGRGLTTLT---PGCEIASWQECDLLERPIGDRHDGDKPLRF 1032 Query: 598 --APFEIKTIYIR 608 AP+EIKT IR Sbjct: 1033 ELAPYEIKTFLIR 1045 >ref|NP_562996.1| alpha-mannosidase [Clostridium perfringens] dbj|BAB81786.1| alpha-mannosidase [Clostridium perfringens str. 13] Length = 1044 Score = 375 bits (962), Expect = e-102, Method: Compositional matrix adjust. Identities = 216/602 (35%), Positives = 327/602 (54%), Gaps = 49/602 (8%) Query: 10 GKQSELKNACNESGGGQPQLPEWRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELWQ 69 GK + E+ +LP+W G+LYLE HRGTYT+ GRNKR+NR E LY AE + Sbjct: 487 GKAKDYFKKLEETVKDNNKLPKWVGELYLEYHRGTYTSMGRNKRDNRVCENLYTSAEKF- 545 Query: 70 TLAGSSIAPELQRSI-AGQLHKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGE 128 +S+A L ++ L+ W+ +LLNQFHDI+PGS+I E Y+ +++EY+++ G Sbjct: 546 ----NSMAMLLGKNYPLADLNAAWETILLNQFHDILPGSSIKEVYDVTKEEYKDLIEKGN 601 Query: 129 SSLQPGLEALTAQVAAAGEGIPYVLFNSLGWERDAVVPVHLPEGRTWAAYDEAGSPLHLD 188 + + L+ +T+++ + V+FNSL +ERD + +PE + +P LD Sbjct: 602 NFVGSALDNITSEINLKERSV--VVFNSLVFERDDIATFDVPE--------DIENPAVLD 651 Query: 189 LAADLSARDXXXXXXXXXXXXXXXXXSIERATEQATGQVTAYIHVPSIPGFGYKTVWLRQ 248 ++ + E+A A G IP GYK+ + + Sbjct: 652 GEEEIICQ---------------RIDDWEKAIFFAKG----------IPANGYKSFKIIE 686 Query: 249 V--DEAVTGSMPERRELNDSWETPHYRLAFNERGEITSLFDKSAGREVVQPGGRANRFHF 306 E S+ E+ ND R+ FNE G I SLFDK A REV++ G NR Sbjct: 687 STNKEVENVSLNEKEAEND-----FLRIKFNEEGNIVSLFDKRAEREVLKEGTLGNRLQA 741 Query: 307 FHDRPTLWDAWDIDSRYEAQPAGEAELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILY 366 F D+P + WDID Y+ + ++ V+ G V+ LR K +S I Q++ +Y Sbjct: 742 FEDKPMEYSNWDIDIYYKEKMWNVDDVQSIEVVEEGPVRSTLRIERKFLESTIVQNIYVY 801 Query: 367 AHDRRIDFKTHVDWNEAHKLLKVGFPVDVVADKATYEIPFGALERPTHRNTSWEQAQYEV 426 RIDF ++VDW E LLK FP+D+ A +ATYEI +G ++RPTH NTSW++A++EV Sbjct: 802 RDLDRIDFNSYVDWKEHEVLLKTSFPIDLNATEATYEIQYGNVKRPTHSNTSWDEARFEV 861 Query: 427 CGHRFADVSEHGYGVSLLNDCKYGYDIQGSTIRLSLLRAPKWPDKVADQGAHDFTYALYP 486 CG ++AD+SE +GVSLLN+CKYG+DIQ +RL+LL++ P DQ H FTY++YP Sbjct: 862 CGQKWADLSEGDFGVSLLNNCKYGHDIQDGDMRLTLLKSGTHPWTETDQEEHFFTYSIYP 921 Query: 487 HTGDWRSAHTLRKAAELNHEVPAIAADGKTTGQLPSTGSFIRFDGQHVVLDTVKPAEDGR 546 H G W+ AHT++KA ELN + + K G L T ++ + +++++ +K AED Sbjct: 922 HMGTWKEAHTVQKAYELNQPLYTRVCE-KHEGILEDTLGLVKVNKDNIMIEVIKKAEDTD 980 Query: 547 GSVLRLYESSGGRETVKLAWNQPFSEIYISNALEAELEPVIHHEGEFELNFAPFEIKTIY 606 ++R+YE R L +++ E + N +E LE + E PFEIKT Sbjct: 981 HLIIRMYEFFNSRTKSTLEFSREIEEAFECNLMERNLEEAKFEGEKIEFTINPFEIKTFK 1040 Query: 607 IR 608 ++ Sbjct: 1041 VK 1042 >ref|NP_923026.1| putative alpha-mannosidase [Gloeobacter violaceus] dbj|BAC88021.1| glr0080 [Gloeobacter violaceus PCC 7421] Length = 1010 Score = 355 bits (912), Expect = 1e-96, Method: Compositional matrix adjust. Identities = 225/588 (38%), Positives = 305/588 (51%), Gaps = 57/588 (9%) Query: 28 QLPEWRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELWQTLAGSSIAPELQRSIAGQ 87 + P W +LYLE HRGT+TTH K+ NR+ E+ EAE + +S + A + Sbjct: 472 RFPTWNSELYLEFHRGTFTTHAGQKQKNRRMEVTLAEAE---KFSAASTVLQSTAYPAAE 528 Query: 88 LHKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGESSLQPGLEALTAQVAAAGE 147 L WK VL NQFHDI+PGSAI YE +++E+RE A GE L AL AQ G Sbjct: 529 LEVIWKKVLFNQFHDILPGSAIPAVYEQADREWREAQAAGEDLRTGALRALAAQADTQGA 588 Query: 148 GIPYVLFNSLGWERDAVVPVHLPEGRTWAAYDEAGSPLHLDLAADLSARDXXXXXXXXXX 207 G+P V+FNSL RDA PV LP A + G+ L +A D + +D Sbjct: 589 GLPVVVFNSLATPRDA--PVALP-----APGNLRGARL---VAVDRTGKDVPV------- 631 Query: 208 XXXXXXXSIERATEQATGQVTAYIH--VPSIPGFGYKTVWLRQVDEAVTGSMPERRELND 265 QA G ++H +PG G+K WLR D + Sbjct: 632 --------------QAAGD---WLHFWAAGVPGIGHKVFWLRPADAPAPATT-------- 666 Query: 266 SWETPHYRLAFNER-GEITSLFDKSAGREVVQPGGRANRFHFFHDRPTLWDAWDIDSRYE 324 E + R+ + R G++ S+FDK GRE + G NR FF D WDAW+ID YE Sbjct: 667 GLENAYLRVEVDPRTGDLASIFDKRTGRETLN--GPGNRLQFFKDEGQYWDAWNIDPDYE 724 Query: 325 AQPAGEAELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILYAHDRRIDFKTHVDWNEAH 384 P LV + G V+ V+R + QS QDLILY ++ VDW E H Sbjct: 725 KHPLEAPTLVSLQTVEQGPVRAVVRVVRRFRQSTFEQDLILYRDAPHLEIANRVDWQERH 784 Query: 385 KLLKVGFPVDVVADKATYEIPFGALERPTHRNTSWEQAQYEVCGHRFADVSEHGYGVSLL 444 +K FP+ V +++ATYEIPF +ER T R+T +AQ+EV R+ADV++ GVSL+ Sbjct: 785 VFVKAAFPLAVRSEQATYEIPFATIERSTLRDTPARKAQWEVPALRWADVADARSGVSLM 844 Query: 445 NDCKYGYDIQ----GSTIRLSLLRAPKWPDKVADQGAHDFTYALYPHTGDWRSAHTLRKA 500 ND KYG+D + G+ +RLSLLR P+WPD AD G H F YALYPH GDWR A T+R Sbjct: 845 NDSKYGHDARRTDTGTLLRLSLLRGPEWPDPKADLGEHSFRYALYPHPGDWREAATVRAG 904 Query: 501 AELNHEVPAIAADGKTTGQLPSTGSFIRFDGQHVVLDTVKPAEDGRGSVLRLYESSGGRE 560 E N + A+ G LP SF++ D VV+ K EDG+G +LRLYES G Sbjct: 905 LEFNTPLHAL-PQTIHPGTLPE--SFLQLDNPQVVVAAWKQVEDGKGWLLRLYESQGQPA 961 Query: 561 TVKLAWNQPFSEIYISNALEAELEPVIHHEGEFELNFAPFEIKTIYIR 608 +L P + ++ LE + + +G+ L P+EI+T+ ++ Sbjct: 962 AARLRLAIPVRSVVKTDLLERPIAALAVQDGQASLQLGPYEIQTVRVQ 1009 >ref|NP_465539.1| similar to alpha-mannosidase [Listeria monocytogenes EGD-e] pir||AG1326 alpha-mannosidase homolog lmo2015 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAD00093.1| lmo2015 [Listeria monocytogenes] Length = 1036 Score = 326 bits (835), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 217/586 (37%), Positives = 297/586 (50%), Gaps = 74/586 (12%) Query: 32 WRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELWQTLAGSSIAPELQRSIA---GQL 88 W G+LY E HRGTYT+ K+ NRK E+ R +E LA L++ I +L Sbjct: 512 WNGELYFEYHRGTYTSQAFTKKMNRKIELGLRNSEWLSVLAN------LKQGITYPTEKL 565 Query: 89 HKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGESSLQPGLEALTAQVAAAGEG 148 W+++L NQFHDIIPGS+I E Y+ + EY E + L +LT AA E Sbjct: 566 AGIWQILLRNQFHDIIPGSSIKEVYDDALIEYTEAMTENNVLISKQLASLTR---AAEEK 622 Query: 149 IPYVLFNSLGWERDAVVPVHLPEGRT--WAAYDEAGSPLHLDLAADLSARDXXXXXXXXX 206 I L+NS W R + + PE A YDE L Sbjct: 623 I--TLWNSSTWTRPRYIEIS-PENPADHLAFYDENNILL--------------------- 658 Query: 207 XXXXXXXXSIERATEQATGQVTAYIHVPSIPGFGYKTVWLRQVDEAVTGSMPERRELNDS 266 Q T I P++P G T+ +++ + S N Sbjct: 659 -------------PAQKLVNNTWLIQHPNLPVLGAATISVKETPNILGQSSFNYN--NKQ 703 Query: 267 WETPHYRLAFNERGEITSLFDKSAGREVVQPGGRANRFHFFHDRPTLWDAWDIDSRYEAQ 326 ETP Y++ +NE G+ TS+FDK R+V+ R N F F D+P DAWDID Y+ + Sbjct: 704 LETPFYQIKWNEVGQFTSIFDKKNNRQVLAENTRGNVFQLFEDKPMWHDAWDIDLFYQEK 763 Query: 327 PAGEAELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILYAHDRRIDFKTHVDWNEAHKL 386 L + G +Q ++ +S+ITQ + Y I F+T VDW + ++L Sbjct: 764 QNEITALDSVEITEKGPLQFTMKQVLHSEKSQITQWIHFYHESPEIRFETEVDWRDRNQL 823 Query: 387 LKVGFPVDVVADKATYEIPFGALERPTHRNTSWEQAQYEVCGHRFADVSEHGYGVSLLND 446 LKV FPVDV A +A+Y+I FG ++RPTH NTSW+ A++E GH++AD+SE YGVSLLND Sbjct: 824 LKVAFPVDVHASEASYDIQFGNVKRPTHWNTSWDYARFETVGHQWADLSEKNYGVSLLND 883 Query: 447 CKYGYDIQGSTIRLSLLRAPKWPDKVADQGAHDFTYALYPHTGDWRSAHTLRKAAELNHE 506 KYGYDI+ STIRL+LL+A +PD AD G H FTY+LYPH GD+ T++ A ELN+ Sbjct: 884 SKYGYDIKDSTIRLTLLKAAGYPDPDADLGIHQFTYSLYPHQGDFLDGETVQAAWELNN- 942 Query: 507 VPAIAADGKTTGQLPSTGSFIRFDGQHVVLDTVKPAEDGRGSVLRLYESSGGRETVKLAW 566 P ++G+ T S + +V++D+VK AEDG G +LRL+E +G V Sbjct: 943 -PIFTSNGEI-----ETFSLFQVTNPYVMIDSVKQAEDGNGFILRLHEFAGTHGEVTFNS 996 Query: 567 NQPFSEIYISNALEAELEPVIHHEGEFEL-----NFAPFEIKTIYI 607 + + I N LE E E L APFEIKT I Sbjct: 997 DYTITSITECNLLE---------ENETSLPTPTFTIAPFEIKTYRI 1033 >ref|NP_471457.1| similar to alpha-mannosidase [Listeria innocua] pir||AI1697 alpha-mannosidase homolog lin2123 [imported] - Listeria innocua (strain Clip11262) emb|CAC97353.1| lin2123 [Listeria innocua] Length = 1032 Score = 325 bits (834), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 207/583 (35%), Positives = 304/583 (52%), Gaps = 64/583 (10%) Query: 32 WRGDLYLELHRGTYTTHGRNKRNNRKAEILYREAELWQTLA----GSSIAPELQRSIAGQ 87 W G+LY E HRGTYT+ K+ NRK E+ R +E LA G++ E + Sbjct: 508 WNGELYFEYHRGTYTSQAFTKKMNRKIELGLRNSEWLSVLANLKEGTTYPTE-------K 560 Query: 88 LHKGWKLVLLNQFHDIIPGSAITEAYETSEKEYREVFALGESSLQPGLEALTAQVAAAGE 147 L W+++L NQFHDIIPGS+I E Y+ + EY E + + LE LT Q A E Sbjct: 561 LANIWQILLRNQFHDIIPGSSIKEVYDDALIEYTEALTENNTIISKQLEKLTQQ---ASE 617 Query: 148 GIPYVLFNSLGWERDAVVPVHLPEGRTWAAYDEAGSPLHLDLAADLSARDXXXXXXXXXX 207 I L+NS W R P ++++ AD A + Sbjct: 618 KI--TLWNSSTWNR----------------------PRYIEIKADNPASNLVFYDQKN-- 651 Query: 208 XXXXXXXSIERATEQATGQVTAYIHVPSIPGFGYKTVWLRQVDEAVTGSMPERRELNDSW 267 +R Q I ++P G ++ +++ +T S ++ Sbjct: 652 ---------KRLPAQKLANGAWLIQHHNLPALGATSISVKETTSELTKSPFTYKD--KQL 700 Query: 268 ETPHYRLAFNERGEITSLFDKSAGREVVQPGGRANRFHFFHDRPTLWDAWDIDSRYEAQP 327 ETP+Y++ +NE G+ TS+FDK R+V+ R N F F D+P DAWDID Y+ + Sbjct: 701 ETPYYQITWNENGQFTSIFDKKNNRQVLAENARGNVFQLFEDKPMWHDAWDIDLFYQEKQ 760 Query: 328 AGEAELVEKYVLHSGKVQDVLRFRWKLGQSEITQDLILYAHDRRIDFKTHVDWNEAHKLL 387 L V+++G ++ ++ +S+ITQ + Y I F+T VDW + ++LL Sbjct: 761 KEITTLDSFEVINNGPLELTIKQVLHSEKSQITQWIHFYHESPEIRFETEVDWRDRNQLL 820 Query: 388 KVGFPVDVVADKATYEIPFGALERPTHRNTSWEQAQYEVCGHRFADVSEHGYGVSLLNDC 447 KV FPV V A +A+Y+I FG ++RPTH NTSW+ A++E GH++ D+SE YGVSLLND Sbjct: 821 KVAFPVSVHASEASYDIQFGNVKRPTHWNTSWDYARFETVGHQWTDLSEKNYGVSLLNDS 880 Query: 448 KYGYDIQGSTIRLSLLRAPKWPDKVADQGAHDFTYALYPHTGDWRSAHTLRKAAELNHEV 507 KYGYDI+ STIRL+LL+A +PD AD G H FTY+L+PH GD+ T++ A ELN+ Sbjct: 881 KYGYDIKDSTIRLTLLKAAGYPDPEADLGLHQFTYSLFPHQGDFLDGKTVQTAWELNN-- 938 Query: 508 PAIAADGKTTGQLPSTGSFIRFDGQHVVLDTVKPAEDGRGSVLRLYESSGGRETVKLAWN 567 P ++G+ S + +V++D+VK AE+G G ++RL+E +G V L + Sbjct: 939 PIFTSNGEI-----EPFSLFQVTNPYVMIDSVKQAENGNGLIIRLHEFAGTHGEVTLTSD 993 Query: 568 QPFSEIYISNALEAELEPVIHHEGEFELNFAPFEIKT--IYIR 608 + I N LE E E + PFEIKT IY++ Sbjct: 994 YAITSITECNLLE-ENETTLETAT---FAITPFEIKTYRIYLK 1032 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 958,756,202 Number of extensions: 41044385 Number of successful extensions: 82791 Number of sequences better than 1.0e-05: 76 Number of HSP's gapped: 82576 Number of HSP's successfully gapped: 128 Length of query: 608 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 476 Effective length of database: 398,844,510 Effective search space: 189849986760 Effective search space used: 189849986760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)