BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0019.p (368 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_782656.1| spore germination protein KB [Clostridium tetan... 200 4e-50 ref|NP_242802.1| spore germination protein KB [Bacillus halodura... 159 1e-37 ref|ZP_00097483.1| COG0814: Amino acid permeases [Desulfitobacte... 129 1e-28 gb|AAB61395.1| germination protein 119 9e-26 ref|NP_149181.1| Spore germination protein, GRKB [Clostridium ac... 111 3e-23 >ref|NP_782656.1| spore germination protein KB [Clostridium tetani E88] gb|AAO36593.1| spore germination protein KB [Clostridium tetani E88] Length = 369 Score = 200 bits (509), Expect = 4e-50, Method: Compositional matrix adjust. Identities = 104/368 (28%), Positives = 197/368 (53%), Gaps = 3/368 (0%) Query: 1 MSKEIIPAGQAISISVLFIIGTSLFMGSSGQSGNSSWXXXXXXXXXXXXXXXXYARLHGL 60 M KE+I Q + + IG L W Y ++ L Sbjct: 5 MKKEVISNKQIQLLIFSYCIGAYLLFSMGAGIKQDIWISSILAIIFTIPIVIMYGKIMNL 64 Query: 61 FPGKDLYDMLILVFGAVAGRMLSCLYIWYALHLGSMVLRNFGEFSKTVALTSTPMLAPML 120 +PGK+ +D+L VFG + G++L+ ++I++A LG+ +LR+F +F K AL STPM+ PM+ Sbjct: 65 YPGKNFFDILEEVFGRMVGKVLNIIFIFHAFFLGAYILRDFADFIKVTALFSTPMIVPMI 124 Query: 121 VIGLLCVWVVKAGMEVLGRSAKFLLLFTIMVIAVIELLSIPKYQYHHLLPVLDSGWKLVL 180 IG+L +W++ G+EVL AKFL+ ++ + +I ++ IP+ +L PV + K +L Sbjct: 125 CIGILSIWILTEGIEVLAAWAKFLIRIILISMILIWIMLIPEMHISNLQPVFYADLKDIL 184 Query: 181 SDTIGSFTFPFAEIVVFLGAFNVLPKKGSAAKVLVSSTMIAGVIILVVTLRNLMVLGPDI 240 + TFPF+E+++FL F+ + V + ++ G+++++ T+ NLM+LG ++ Sbjct: 185 KQALHLITFPFSEVIIFLNFFDYVNYNSKTKNVFIKPLILGGMLVVLYTMINLMLLGGEL 244 Query: 241 LSSLYFPSYVAVGRINIGDFLTRIEGSSAIIFVTALFIKVSLCLYAASNGMAKVFKLDSY 300 S Y+P Y ++ R+N+G+ R+E +I F F++++ C+ S G+ KVF L Y Sbjct: 245 YSFFYYPGYESIKRLNLGNEFQRLEIIVSIAFTIIQFLEINFCILGVSKGITKVFNLKEY 304 Query: 301 RSVVLQLGLIMVYLAVFIYKDIMQMQYFAYHIYKIYALPFQVAIPVILWVTAEILAWRRS 360 R V++ + ++++ ++ M+ ++ Y + Q+ +P I+++ A I +R Sbjct: 305 RRVLIPVIILLITFDYVMFGSAMEAFEIVRELWPAYGIVIQIVLPFIIFIFATI---KRK 361 Query: 361 GKKTAAQQ 368 + + Sbjct: 362 NQLVKCNK 369 >ref|NP_242802.1| spore germination protein KB [Bacillus halodurans] pir||H83891 spore germination protein KB gerKB [imported] - Bacillus halodurans (strain C-125) dbj|BAB05655.1| spore germination protein KB [Bacillus halodurans] Length = 373 Score = 159 bits (402), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 105/346 (30%), Positives = 182/346 (52%), Gaps = 12/346 (3%) Query: 10 QAISISVLFIIGTSLFMGSSGQSGNSSWXXXXXXXXXXXXXXXXYARLHGLFPGKDLYDM 69 Q S+ VLF +G+++ +G +G +W LH FP Sbjct: 11 QFFSMIVLFELGSAIVVGVGLDAGQDAWMTVLIAMLAGLVLYCVLYYLHSHFPSDTWIAY 70 Query: 70 LILVFGAVAGRMLSCLYIWYALHLGSMVLRNFGEFSKTVALTSTPMLAPMLVIGLLCVWV 129 L V G + LY Y +++ + VLR+FG+ T LT TP++ L++ + V+V Sbjct: 71 LQKVLTVPIGWFIGFLYCLYFIYIATRVLRDFGDLLTTSMLTDTPLMVINLLMVFVIVYV 130 Query: 130 VKAGMEVLGRSAKFLL--LFTIMVIAVIELLSIPKYQYHHLLPVLDSGWKLVLSDTIG-S 186 + G+ VL R+ +F L LF I +V + S Y +L+PVL++GW VL + + Sbjct: 131 LFHGINVLARTGEFFLIILFFIGFFSVTLIFSTDLYNIKNLMPVLENGWMPVLREAFPVT 190 Query: 187 FTFPFAEIVVFLGAFNVLPKKGSAAKVLVSSTMIAGVIILVVTLRNLMVLGPDILSSLYF 246 FTFPF EI+VF F+ L KK ++ + + ++G+++ + T+ ++VLG DI + F Sbjct: 191 FTFPFGEIIVFTMLFHFLNKKEMGLRIGLLAIGMSGLLLALTTILYIIVLGVDITNRATF 250 Query: 247 PSYVAVGRINIGDFLTRIEGSSAIIFVTALFIKVSLCLYAASNGMAKVFKLDSYRSVVLQ 306 P VG+I DF+ R++ + + V A+F KV++ YA G +++FK+D ++ ++ Sbjct: 251 PLLTMVGKIQAADFIERLDALAVTVLVVAMFFKVAIFFYAGVAGFSELFKVDKFQKLLPL 310 Query: 307 LGLIMVYLAVFIYKDIMQ-----MQYFAYHIYKIYALPFQVAIPVI 347 G I+V+ +V I + + ++ Y+++ LPFQV IP+I Sbjct: 311 FGAILVFNSVHIAANFSEHLKEGLEVVPYYLH----LPFQVGIPLI 352 >ref|ZP_00097483.1| COG0814: Amino acid permeases [Desulfitobacterium hafniense] Length = 336 Score = 129 bits (323), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 95/340 (27%), Positives = 167/340 (49%), Gaps = 5/340 (1%) Query: 26 MGSSGQSGN-SSWXXXXXXXXXXXXXXXXYARLHGLFPGKDLYDMLILVFGAVAGRMLSC 84 M S Q N +W Y L FP +L ++ LV+G + G+++S Sbjct: 1 MPYSTQIINQDNWLAVIIGFILSLPMVLIYIWLAQKFPQNNLLEIHDLVYGPLIGKLISA 60 Query: 85 LYIWYALHLGSMVLRNFGEFSKTVALTSTPMLAPMLVIGLLCVWVVKAGMEVLGRSAKFL 144 YI + +L L G+F T L TPM +L+ +C W V+ G+EV+ R Sbjct: 61 AYIVFIFNLLIYNLVYVGDFLLTYLLPETPMWIVLLMFTFVCAWAVRLGLEVIARVNPLF 120 Query: 145 LLFTIMVIAVIELLSIPKYQYHHLLPVLDSGWKLVLSDTIGSFTFPFAEIVVFLGAFNVL 204 ++ T+ + L IP+ + +L+P+ D+ + T P+ EIV+FL + Sbjct: 121 VVITLFITFSTMTLLIPEMDFSNLIPLFDTPMLDFIQGVHIMITIPYLEIVIFLFFLPSV 180 Query: 205 PKKGSAAKVLVSSTMIAGVIILVVTLRNLMVLGPDILSSLYFPSYVAVGRINIGDFLTRI 264 K ++ + +L+ +RN VLG ++ L PSY ++ I+IG++LTR+ Sbjct: 181 NNKKQIKHSILFGFCFGSLFLLLPIIRNTTVLG-SLVGILTSPSYESIRLIDIGNYLTRL 239 Query: 265 EGSSAIIFVTALFIKVSLCLYAASNGMAKVFKLDSYRSVVLQLGLIMVYLAVFIYKDIMQ 324 E AI + LFIK SL Y G+A++ +L SY +V+ LG+I + L++ +++ + Sbjct: 240 EVLYAITLIIMLFIKSSLFYYVTVLGIAQLLRLRSYLPLVVPLGIIGICLSLIVHESSLL 299 Query: 325 MQYFAYHIYKIYALPFQVAIPVILWVTAEILAWRRSGKKT 364 ++ +ALPF+V +P++ + A++ R+ K T Sbjct: 300 NIDTTVDVWPFFALPFEVFLPILTLLLAKV---RKLPKHT 336 >gb|AAB61395.1| germination protein Length = 329 Score = 119 bits (299), Expect = 9e-26, Method: Compositional matrix adjust. Identities = 88/330 (26%), Positives = 161/330 (48%), Gaps = 9/330 (2%) Query: 1 MSKEIIPAGQAISISVLFIIGTSLFMGSSGQSGNSSWXXXXXXXXXXXXXXXXYARLHGL 60 M K I A Q + VLF +G++L + + + +W Y +L+ Sbjct: 1 MEKAKISATQLFILMVLFELGSALLVPLAMGAKQDAWLAILIGMLCSFFFFLIYHKLYSY 60 Query: 61 FPGKDLYDMLILVFGAVAGRMLSCLYIWYALHLGSMVLRNFGEFSKTVALTSTPMLAPML 120 +P + + + G V G +L+ +YI Y L+ S VLR+FGE T A TP+ Sbjct: 61 YPDLLPTEYMQKILGKVLGTLLAFIYIVYFLYDASRVLRDFGEMLLTFAYPDTPLFIANA 120 Query: 121 VIGLLCVWVVKAGMEVLGRSAKFLLLFT--IMVIAVIELLSIPKYQYHHLLPVLDSG--- 175 ++ L+ +++ + G+E + RS + L +F + + I ++S + +L PVL G Sbjct: 121 LLMLVIIYITRKGIEAIARSGELLFIFMYFLAIAGFILIISSGLIDFKNLQPVLKEGILP 180 Query: 176 -WKLVLSDTIGSFTFPFAEIVVFLGAFNVLPKKGSAAKVLVSSTMIAGVIILVVTLRNLM 234 K+ L++T+ FPF E +VF L A ++ +T ++G+ +++ + N+ Sbjct: 181 VAKVALTETL---YFPFTEAIVFTMLLPYLSNPKKANVTMLCATGLSGINLIITMIINIS 237 Query: 235 VLGPDILSSLYFPSYVAVGRINIGDFLTRIEGSSAIIFVTALFIKVSLCLYAASNGMAKV 294 VLG D+ + FP V I + DFL R++ V ++ KVS+ YA+ A + Sbjct: 238 VLGVDLTTRSQFPLLSTVQTIQVADFLERLDVFFMFAMVIGIYFKVSILFYASIISTATL 297 Query: 295 FKLDSYRSVVLQLGLIMVYLAVFIYKDIMQ 324 FK+ S + LG+I+++L++ I + + Sbjct: 298 FKIKSPSRLAYPLGIIVLFLSITIASNFQE 327 >ref|NP_149181.1| Spore germination protein, GRKB [Clostridium acetobutylicum] gb|AAK76763.1| Spore germination protein, GRKB [Clostridium acetobutylicum] Length = 364 Score = 111 bits (277), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 99/362 (27%), Positives = 173/362 (47%), Gaps = 9/362 (2%) Query: 8 AGQAISISVLFIIGTSLFMGS--SGQSGNSSWXXXXXXXXXXXXXXXXYARLHGLFPGKD 65 + + +S+ +L +I S F S + +W Y L FPGK Sbjct: 9 SSKQLSLIMLSLIEASTFTAVFVSQYTKQDTWVVLISGFIIVLFLLFMYVSLCSKFPGKT 68 Query: 66 LYDMLILVFGAVAGRMLSCLYIWYALHLGSMVLRNFGEFSKTVALTSTPMLAPMLVIGLL 125 + ++ ++G + ++S LYI+Y + R +F T ++ ++ ++ +L Sbjct: 69 IIEIHAKIYGNLLVNLISLLYIFYFWFIIPANFRFLADFFSTYLSQNSDIIPFIIPAAIL 128 Query: 126 CVWVVKAGMEVLGRSAKFLLLFTIMVIAVIELLSIPKYQYHHLLPVLDSGWKLVLSDTIG 185 C++ V+ G EV+ R + L + T+M+ +I L +LLP L + T Sbjct: 129 CIYAVRKGAEVISRVSSILSILTVMLSIIITALIAKDIHISNLLPPLQLNLGQFVQGTNI 188 Query: 186 SFTFPFAEIVVFLGAFNVLPKKGSAAKVLVSSTMIAGVIILVVTLRNLMVLGPDILSSLY 245 P EI+VF+ F L + K + +++ + L V +RN +VLG +SSL Sbjct: 189 MVAIPLGEIIVFMIFFPHLNDQTKLRKSAFTGLILSFIFFLFVLVRNTLVLGN--VSSLQ 246 Query: 246 F-PSYVAVGRINIGDFLTRIEGSSAIIFVTALFIKVSLCLYAASNGMAKVFKLDSYRSVV 304 P+Y IN+G+ +TR+E ++A+ + L +K+ LYA +A+ FKL SY+ +V Sbjct: 247 VQPAYQVSSLINVGEVITRVEVTTAVFLLFNLLMKICFFLYATVISIAQCFKLRSYKPLV 306 Query: 305 LQLGLIMVYLAVFIYKDIMQMQYFAYHIYKIYALPFQVAIPVILWVTAEILAWRRSGKKT 364 + I L++ ++K + Y A IY IYAL F V +P +T+ ILA + KT Sbjct: 307 FPVTAISAVLSITMFKSPLDQLYIASDIYPIYALLFIVILP----ITSFILASIKKSSKT 362 Query: 365 AA 366 A+ Sbjct: 363 AS 364 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.329 0.142 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 468,780,705 Number of extensions: 17448263 Number of successful extensions: 67451 Number of sequences better than 1.0e-05: 43 Number of HSP's gapped: 67411 Number of HSP's successfully gapped: 43 Length of query: 368 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 241 Effective length of database: 412,420,005 Effective search space: 99393221205 Effective search space used: 99393221205 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 126 (53.1 bits)