BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0236.0017.p (384 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00097484.1| hypothetical protein [Desulfitobacterium hafn... 212 1e-53 ref|NP_782657.1| spore germination protein KC [Clostridium tetan... 159 8e-38 ref|NP_149183.1| Spore germination protein, GRKC [Clostridium ac... 158 2e-37 ref|ZP_00103926.1| hypothetical protein [Desulfitobacterium hafn... 139 8e-32 ref|NP_782369.1| spore germination protein KC [Clostridium tetan... 125 1e-27 >ref|ZP_00097484.1| hypothetical protein [Desulfitobacterium hafniense] Length = 389 Score = 212 bits (539), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 113/365 (30%), Positives = 210/365 (57%), Gaps = 13/365 (3%) Query: 13 CIVMLPCLSGCWNYTEVDDISIVAGVAIDKDGANEKIQLTAEMVDIQ-------GGLDQN 65 C ++ ++GCWN E++ ++IV G+ IDK +IQ+TA++ + + GG D Sbjct: 14 CTPLILNITGCWNQRELNSLAIVMGLGIDKAQEPNQIQVTAQIANPKALKSSSLGGPDSQ 73 Query: 66 QAGFKMLSLTGSTIFDIVRNMISITGKKLFWSHAKAIIFSEKVAREGLIRTVDWYSRDTE 125 F + TG T+F I+R+ + +KL++ H + +I A EG+ + +D+++RD E Sbjct: 74 --AFWNIQNTGDTLFSILRDFTHESNRKLYFPHIQVLILGRDYAEEGIQKELDFFARDHE 131 Query: 126 TRSDVFIFVSGEPTAREILNLNSTTKTIMSFELAQMMRDEKYTSSAPTVEIWDFIDKLET 185 TR ++++ VS E AR++LN+ I + +++++ D+ S T ++ DF+ L + Sbjct: 132 TRLNIWLVVS-EGNARDVLNIKPVIDRIPAINISKLLDDQDPNSQTSTSQLNDFLSDLIS 190 Query: 186 MGNHAVAPLIYIHEQNGQKNERVNGCAIFSKDKMVGKLNGKETKSMLFAKDGIKGGVLGV 245 APL+ I + G ++ +V G A+F DK+VG+LN ET+ +L+A+D +K G++ V Sbjct: 191 PTTAPTAPLVEIIRKGGNESLQVKGTAVFKGDKLVGELNKYETRGLLWARDEVKSGIIDV 250 Query: 246 NDKNGVPTYSLEILSSRTKVKPRVVNGKLLLQVRTVTHTGLDEVMTSESFSGNETISAIE 305 SLEI+ S+TKV +V+ +++++V L +E+ E ++AIE Sbjct: 251 ECPECGSKVSLEIIDSKTKVSAEIVDNQVIMKVYIQEKGALAAQYCAENLRTKEKVAAIE 310 Query: 306 ERAGEALEQEILAVIRKMQREYHADIFGYGEIIHEHQPEMWVKVRDRWDDEFAKL--DIT 363 A++ E+LA +K Q E +ADIFG+GE++ + + W +++ WD+ F ++ ++T Sbjct: 311 RNKAAAIQDEVLAAFKKAQ-ELNADIFGFGEVVRKKHLKEWKNMQEHWDEIFPEIIVEVT 369 Query: 364 VDSKV 368 VD+ + Sbjct: 370 VDASI 374 >ref|NP_782657.1| spore germination protein KC [Clostridium tetani E88] gb|AAO36594.1| spore germination protein KC [Clostridium tetani E88] Length = 383 Score = 159 bits (403), Expect = 8e-38, Method: Compositional matrix adjust. Identities = 103/362 (28%), Positives = 197/362 (54%), Gaps = 4/362 (1%) Query: 22 GCWNYTEVDDISIVAGVAIDKDGANEKIQLTAEMVDIQGGLDQNQAGFKMLSLTGSTIFD 81 GCW+Y+E++D VAG+A+D+D + +T E+++ G Q ++ ++ I Sbjct: 23 GCWDYSEMEDFKHVAGIAVDRDKDKNEYIVTVEILETYPGSKQLKS--HVVQSRDKIIHA 80 Query: 82 IVRNMISITGKKLFWSHAKAIIFSEKVAREGLIRTVDWYSRDTETRSDVFIFVSGEPTAR 141 R+ I G +L SHA+ I S+ +AREG++ +D +RD E R+D++I VS E A Sbjct: 81 AFRDAIKKIGNRLQLSHARVFIVSKDIAREGIVSAIDLINRDVEVRNDMWIAVSKEDLAS 140 Query: 142 EILNLNSTTKTIMSFELAQMMRDEKYTSSAPTVEIWDFIDKLETMGNHAVAPLIYIHEQN 201 EIL + + + I+S+++ +++ VE + D L T G + P++ + +++ Sbjct: 141 EILTKSKSEQEIISYDIEAAIKNSAKVGKYIGVETFKLTDTLLTEGVSPILPMVKLAKKD 200 Query: 202 GQKNERVNGCAIFSKDKMVGKLNGKETKSMLFAKDGIKGGVLGVNDKNGVPTYSLEILSS 261 G V+ A+F DK+VG+L +ET + F + V D +G T SLEIL S Sbjct: 201 GNMGFEVSETAVFKGDKIVGELTERETMMLQFFNGKDLEFIFPV-DSDGNGTVSLEILGS 259 Query: 262 RTKVKPRVVNGKLLLQVRTVTHTGLDEVMTSE-SFSGNETISAIEERAGEALEQEILAVI 320 + ++K + K+++ T + E+ S ++ E + ++ RA E ++ + ++ Sbjct: 260 KREIKVNLNEDKIVMNTYIDIDTAISELADSGINYINEEERNKLKNRAEEQIKSDCNLLV 319 Query: 321 RKMQREYHADIFGYGEIIHEHQPEMWVKVRDRWDDEFAKLDITVDSKVIIESTAKTSRSI 380 K++++Y +DI G+G+ + + +P+ W KV + WD+ F LDI + + I+ + T ++I Sbjct: 320 EKLKKQYKSDIIGFGKELKKKKPQKWKKVSNNWDEIFESLDINISVNIDIKYSGLTRKNI 379 Query: 381 KM 382 ++ Sbjct: 380 EV 381 >ref|NP_149183.1| Spore germination protein, GRKC [Clostridium acetobutylicum] gb|AAK76765.1| Spore germination protein, GRKC [Clostridium acetobutylicum] Length = 394 Score = 158 bits (400), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 96/380 (25%), Positives = 199/380 (52%), Gaps = 14/380 (3%) Query: 13 CIVMLPCLSGCWNYTEVDDISIVAGVAIDKDGANEKIQLTAEMVDIQGGLDQNQAGFKM- 71 CI++ L+ C + E++ ++IV V IDK + ++T ++ + + + Sbjct: 14 CILIAVNLTSCIDSRELNKLAIVLAVGIDKLENCDDTEMTIQLAKLSSNKSGSISSGGSA 73 Query: 72 ---------LSLTGSTIFDIVRNMISITGKKLFWSHAKAIIFSEKVAREGLIRTVDWYSR 122 L+ TG+ + IV+ ++LF+SH + I+ E A+ G+ + +D++ R Sbjct: 74 PSANNVTLNLTETGNGLGKIVKTFNRKLDRQLFFSHNQVIVLGESAAKSGIAKYLDFFMR 133 Query: 123 DTETRSDVFIFVSGEPTAREILNLNSTTKTIMSFELAQMMRDEKYTSSAPTVEIWDFIDK 182 ETR V++ ++ + ++IL++ + LA+++ ++K S P + + DF ++ Sbjct: 134 YRETRLLVWLLIT-KSKVKDILSVPPNMEDTQGRNLAELISEQKEVSEIPAINLKDFTER 192 Query: 183 LETMGNHAVAPLIYIHEQN--GQKNERVNGCAIFSKDKMVGKLNGKETKSMLFAKDGIKG 240 + + + + P++ I N G K R+ A+F KDKM+G L+ ++T+ +L+A + +K Sbjct: 193 IMSKTSAPIIPIVEISTNNSAGTKALRLTSTAVFKKDKMIGTLDSQQTRGLLWALNKVKT 252 Query: 241 GVLGVNDKNGVPTYSLEILSSRTKVKPRVVNGKLLLQVRTVTHTGLDEVMTSESFSGNET 300 GV+ VN T +E + S+ K+ ++ + KL++++ L E +SE+ + + Sbjct: 253 GVVPVNLPGYKGTIDVETIHSKGKIAAKLKDNKLIMKINIKQEGNLLEQNSSENLATPDN 312 Query: 301 ISAIEERAGEALEQEILAVIRKMQREYHADIFGYGEIIHEHQPEMWVKVRDRWDDEFAKL 360 IE+ E ++++I A I K R+ +ADIFG+G+ I+ P+ + K+ +W+ F L Sbjct: 313 FKVIEKAEEETIKKQIEAAILK-SRDLNADIFGFGDAIYRRHPKYFKKIEPQWNKTFKNL 371 Query: 361 DITVDSKVIIESTAKTSRSI 380 +I V+ + + S+ I Sbjct: 372 NIEVNVNCKLRRNGRISKPI 391 >ref|ZP_00103926.1| hypothetical protein [Desulfitobacterium hafniense] Length = 392 Score = 139 bits (351), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 86/368 (23%), Positives = 179/368 (48%), Gaps = 18/368 (4%) Query: 21 SGCWNYTEVDDISIVAGVAIDKDGANEKIQLTAEMVDIQGGLDQNQAG---FKMLSLTGS 77 GCW++ E++++ +V+ + +D + KIQ+T ++++ Q + + Q G ++L+ G Sbjct: 22 PGCWDHRELNELGVVSALGLDLN-ERGKIQITVQVINPQAYITKGQEGKAQTRILTSEGE 80 Query: 78 TIFDIVRNMISITGKKLFWSHAKAIIFSEKVAREGLIRTVDWYSRDTETRSDVFIFVSGE 137 T FD VR +++ +G +LF+ H + I+ E +AR G+ +D+ RD E R ++ ++ E Sbjct: 81 TFFDAVRGLMTKSGDRLFYPHNQVIVIGEDLARAGVKPILDFLERDPEIRLFTWVLLT-E 139 Query: 138 PTAREILNLNSTTKTIMSFELAQMMRDEKYTSSAPTVEIWDFIDKLETMGNHAVAPLIYI 197 A E++ S I + + ++ D S + I +F + + G V I Sbjct: 140 GKAEEVVRAPSDESLISAMHVMLLVDDYYSASKSIPRNILEFATMVYSEGIEPVLGKIEF 199 Query: 198 HEQNGQKNERVNGCAIFSKDKMVGKLNGKETKSMLFAKDGIKGGVLGVNDKNGVPTY--- 254 +E+ G ++ G F DK++ L+ E + L+ + + GG++ K G Sbjct: 200 YEEKGNPTYQLIGGGAFKGDKLIDWLDAGEARGYLWVHNDVNGGIITAYTKEGAENQAQA 259 Query: 255 ----------SLEILSSRTKVKPRVVNGKLLLQVRTVTHTGLDEVMTSESFSGNETISAI 304 S EI+ ++++VKP + KL + L E ++ E + Sbjct: 260 DGSSGLGERISFEIIRTKSRVKPVLEGEKLSFVIDLDVTCNLGERTDNKETITKEDFKEL 319 Query: 305 EERAGEALEQEILAVIRKMQREYHADIFGYGEIIHEHQPEMWVKVRDRWDDEFAKLDITV 364 E+ +A+ E+ ++ K Q+E +DI G+G P+ W + +++W++ F +L++ + Sbjct: 320 EKILSQAVIGEVQGIVDKAQKELRSDILGFGLTTKRKYPDYWNQHKEQWEEMFPELEVEI 379 Query: 365 DSKVIIES 372 K +++ Sbjct: 380 LVKAVVDG 387 >ref|NP_782369.1| spore germination protein KC [Clostridium tetani E88] gb|AAO36306.1| spore germination protein KC [Clostridium tetani E88] Length = 393 Score = 125 bits (315), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 93/366 (25%), Positives = 176/366 (48%), Gaps = 11/366 (3%) Query: 14 IVMLPCLSGCWNYT----EVDDISIVAGVAIDKDGANEKIQLTAEMVDIQGGLDQN-QAG 68 +V S C NY ++D +++ V ID + + V + N ++ Sbjct: 10 LVFSLSFSSCENYKIERKDIDKTMLISVVGIDVSPEGKVLVTICPKVASTSENNSNVESK 69 Query: 69 FKMLSLTGSTIFDIVRNMISITGKKLFWSHAKAIIFSEKVAREGLIRTVDWYSRDTETRS 128 +++ G TIF+ VR + + + + F + + +I E+ A++ +I+ +D++SRD E R Sbjct: 70 SYIINSEGDTIFEAVRKISTASDTRPFLGNVEHVIIGEEAAKKDIIKYLDYFSRDHEFRL 129 Query: 129 DVFIFVSGEPTAREILNLNSTTKTIMSFELAQMMRDEKYTSSAPTVEIWDFIDKLETMGN 188 ++ +FV+ TA+EIL S + I+S L ++ + S + +E+ D + K + Sbjct: 130 NIKVFVTKGCTAKEILERASNSNMILSDYLKSLLSNVDNNSISSEIELSDLMHKFDNKFL 189 Query: 189 HAVAPLIYIHEQN----GQKNERVNGCAIFSKDKMVGKLNGKETKSMLFAKDGIKGGVLG 244 P + I + N Q + +NG AIF K K++G L KE + + +A + +K GVL Sbjct: 190 SPYIPFLVIGDSNDTTINQFSINLNGYAIFKKAKLIGYLADKEARGLNWAIEKVKSGVLI 249 Query: 245 VNDKNGVPTYSLEILSSRTKVKPRVVNGKLLLQVRTVTHTGLDEVMTSESFSGNETISAI 304 V D +G +LEI + +K+ P+ KL + ++ T + + E+ E + S + Sbjct: 250 VKDIHG-DNIALEITEASSKIIPQSSKEKLSILIKVHTSSNIAEITGREDIFTKDYFSYL 308 Query: 305 EERAGEALEQEILAVIRKMQREYHADIFGYGEIIHEHQPEMWVKVRDRWDDEFAKLDITV 364 E ++ EI + + + ++DIF I P W ++ WD F ++DI V Sbjct: 309 EREQNNIVKSEIESAM-DFSNKNNSDIFDINGAIFRKYPMKWQTIQKEWDKIFPEIDIKV 367 Query: 365 DSKVII 370 + +I Sbjct: 368 EVNSLI 373 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 513,196,750 Number of extensions: 20675905 Number of successful extensions: 50878 Number of sequences better than 1.0e-05: 45 Number of HSP's gapped: 50791 Number of HSP's successfully gapped: 46 Length of query: 384 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 257 Effective length of database: 412,420,005 Effective search space: 105991941285 Effective search space used: 105991941285 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)