BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0234.0002.p (354 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_275267.1| conserved protein [Methanothermobacter thermaut... 228 1e-58 ref|ZP_00054985.1| COG5012: Predicted cobalamin binding protein ... 204 3e-51 ref|ZP_00056033.1| COG5012: Predicted cobalamin binding protein ... 187 4e-46 ref|ZP_00129026.1| COG5012: Predicted cobalamin binding protein ... 159 1e-37 gb|EAH65304.1| unknown [environmental sequence] 91 4e-17 >ref|NP_275267.1| conserved protein [Methanothermobacter thermautotrophicus] pir||F69032 conserved hypothetical protein MTH124 - Methanobacterium thermoautotrophicum (strain Delta H) gb|AAB84630.1| conserved protein [Methanothermobacter thermautotrophicus str. Delta H] Length = 314 Score = 228 bits (581), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 122/304 (40%), Positives = 181/304 (59%), Gaps = 3/304 (0%) Query: 48 QDSLYHFRYLAQSVALDSPLLFINYIIWLKVLLAQYKITAEDLQINLDLMKEAISSQVET 107 QD YH YL +++ SP LF +Y++W VLL + E L+ ++ ++ AI + Sbjct: 12 QDINYHLDYLEEAILNSSPELFSDYVLWADVLLKNLGLPEECLRGSIKSIERAIMKLADD 71 Query: 108 PEKELILSYLDMGLHHTRGEESLPTFLQPEKPYYREAEEYLKLLLAGERRKASEFVLGLH 167 + Y+ L + L ++P AEEYL+L+L E R+A E ++ Sbjct: 72 EVADKASEYISGALKILETSHPSSSHLG-DQPLRELAEEYLRLVLNTEAREARELIVSSL 130 Query: 168 EGGVPIRDLYLHLFQNSQYEIGRLWQLGRITVAQEHYCTACTQSIISQLYPRWIQAAQTG 227 E G+ + D+YL +F+ Q+EIGRLWQ +I+VA EHY T+ TQSI+S+LYP A++ Sbjct: 131 ESGIRVEDIYLQVFEPVQHEIGRLWQTNQISVAHEHYATSVTQSIMSELYPYIYSASERR 190 Query: 228 GKTLVAVGVGEELHEIGLRMLADFFEMEGWNTCYLGSNMPAEGLIRYLKEQPADLLAVSV 287 LVA V ELHEIG+RM++DFFEM GW + YLG+N P E + + DL+A+S Sbjct: 191 NLKLVAACVNNELHEIGIRMVSDFFEMNGWESIYLGANTPPEDFSGIVSDLKPDLVALSA 250 Query: 288 TMAYHVSEVQRLIASIRTHAGLDRIKILVGGMPFNIDRTLWEKVGADGYAPDAKGALEVA 347 TM ++VS V+ +I + DR ++VGG PFN+D LW+KVGAD +A DA GA++VA Sbjct: 251 TMTFNVSHVRHIIELLDELE--DRPPVMVGGYPFNVDIKLWKKVGADLHAADASGAVKVA 308 Query: 348 SQLI 351 + + Sbjct: 309 EEFL 312 >ref|ZP_00054985.1| COG5012: Predicted cobalamin binding protein [Magnetospirillum magnetotacticum] Length = 354 Score = 204 bits (519), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 124/338 (36%), Positives = 188/338 (55%), Gaps = 10/338 (2%) Query: 18 QLAEEVTLRQYERQPELRQRFGPSGVARTKQDSLYHFRYLAQSVALDSPLLFINYIIWLK 77 Q AEEVT Y R PE +FGP G D YLA + A + LF Y+ WL+ Sbjct: 15 QTAEEVTALLYSRHPEWDVKFGPKGRKACADDVYNTLLYLATATATERYDLFREYLSWLR 74 Query: 78 VLLAQYKITAEDLQINLDLMKEAISSQVETPEKELILSYLDMGLHH--TRGEE--SLPTF 133 LLAQ I L L M++A++ + P+ L++LD G+ + G E SLP F Sbjct: 75 RLLAQINIAPRHLADMLVAMRDALAGRGMNPDSPE-LTFLDDGISDLASLGAETGSLP-F 132 Query: 134 LQPEKPYYREAEEYLKLLLAGERRKASEFVLGLHEGGVPIRDLYLHLFQNSQYEIGRLWQ 193 + + A E LL G+R A+ VL E + + +YL + + EIGR W Sbjct: 133 AETHRADL--ASELFSLLHRGDRGTATTSVLNWAET-LGVEGVYLDVIEPCLQEIGRRWH 189 Query: 194 LGRITVAQEHYCTACTQSIISQLYPRWIQAAQTGGKTLVAVGVGEELHEIGLRMLADFFE 253 ++ VA+EH+ TA Q ++++LYP QA++ G + LVAV ELHEIG+RM+AD F+ Sbjct: 190 DNQLNVAEEHFMTASIQVVMARLYPLVFQASRIG-RRLVAVCAEGELHEIGMRMVADLFD 248 Query: 254 MEGWNTCYLGSNMPAEGLIRYLKEQPADLLAVSVTMAYHVSEVQRLIASIRTHAGLDRIK 313 + GW+T +LG+ +P +IR ++++ DLLA+S ++A +V V RL++++R +K Sbjct: 249 IGGWDTTFLGAGVPDTAVIRTIRDRGCDLLAISASLAINVPAVTRLVSALRLDPQCSHVK 308 Query: 314 ILVGGMPFNIDRTLWEKVGADGYAPDAKGALEVASQLI 351 ++VGG FN LW + GADG+A + AL ++L Sbjct: 309 VMVGGRAFNQSPDLWRRTGADGWAGGGREALRTGNRLF 346 >ref|ZP_00056033.1| COG5012: Predicted cobalamin binding protein [Magnetospirillum magnetotacticum] Length = 240 Score = 187 bits (474), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 95/219 (43%), Positives = 139/219 (63%), Gaps = 2/219 (0%) Query: 131 PTFLQPEKPYYREAEEYLKLLLAGERRKASEFVLGLHEGGVPIRDLYLHLFQNSQYEIGR 190 P L A+ YL L+A R +A+ ++ +GG+ I ++YL +FQ + +E+GR Sbjct: 20 PVHLDGTGGLSPMAQRYLNTLIARRRHEATAMIMEAVDGGMSIENIYLDVFQPALWEVGR 79 Query: 191 LWQLGRITVAQEHYCTACTQSIISQLYPRWIQAAQTGGKTLVAVGVGEELHEIGLRMLAD 250 LWQ+ R+T+AQEHY TA TQ+I+SQLYPR I +T+VA V ELHE+G RM+AD Sbjct: 80 LWQIDRLTIAQEHYITAVTQAIMSQLYPR-IFRGDGHRRTMVACCVSGELHEVGARMVAD 138 Query: 251 FFEMEGWNTCYLGSNMPAEGLIRYLKEQPADLLAVSVTMAYHVSEVQRLIASIRTHAGLD 310 FEM GW++ YLG+N+PA ++ A+++A+S T+ H+ V+ L+ R G Sbjct: 139 CFEMSGWDSHYLGANIPASDVVDMALSAEAEVIAISATLTTHLGLVEDLVTKARKQFG-S 197 Query: 311 RIKILVGGMPFNIDRTLWEKVGADGYAPDAKGALEVASQ 349 + ILVGG PFNID+ LW K+GADG A +A A+ +A + Sbjct: 198 KTTILVGGYPFNIDQDLWRKLGADGSAQNAAEAVMIAER 236 >ref|ZP_00129026.1| COG5012: Predicted cobalamin binding protein [Desulfovibrio desulfuricans G20] ref|ZP_00131270.1| hypothetical protein [Desulfovibrio desulfuricans G20] Length = 235 Score = 159 bits (402), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 85/209 (40%), Positives = 118/209 (56%), Gaps = 1/209 (0%) Query: 144 AEEYLKLLLAGERRKASEFVLGLHEGGVPIRDLYLHLFQNSQYEIGRLWQLGRITVAQEH 203 A YL+L+L R A + G + D+YL + Q YE GRLWQ I +A EH Sbjct: 28 AGTYLELMLQARRNDALRCIQKAIARGASLSDIYLKVLQPVMYETGRLWQTNAIDIAVEH 87 Query: 204 YCTACTQSIISQLYPRWIQAAQTGGKTLVAVGVGEELHEIGLRMLADFFEMEGWNTCYLG 263 Y TA TQ ++ ++P + +TG K +V +G ELH++GL ML D FE EGW+T ++G Sbjct: 88 YITAATQLTMAHIFPHALSKKRTG-KKMVGGCLGSELHQLGLHMLCDLFESEGWDTYFMG 146 Query: 264 SNMPAEGLIRYLKEQPADLLAVSVTMAYHVSEVQRLIASIRTHAGLDRIKILVGGMPFNI 323 + P + + + +Q DLL +SVTM V EV+ I +R G D KILVGG+PF + Sbjct: 147 AATPGKSFLTVVSDQHPDLLCISVTMQRGVPEVRETIELLRAKLGADCPKILVGGLPFIL 206 Query: 324 DRTLWEKVGADGYAPDAKGALEVASQLIS 352 L VGADG D A+ +A+QL S Sbjct: 207 SPGLAAAVGADGTGTDGAQAVALATQLTS 235 >gb|EAH65304.1| unknown [environmental sequence] Length = 190 Score = 90.9 bits (224), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 57/183 (31%), Positives = 95/183 (51%), Gaps = 13/183 (7%) Query: 176 LYLHLFQNSQYEIGRLWQLGRITVAQEHYCTACTQSIISQLYPRWIQAAQTGGKTLVAVG 235 +YL L +N+ IG LW G+I +++EH T ++ ++ + + Q G + Sbjct: 12 IYLDLIRNTLNHIGNLWHEGKILISEEHRATQFCLLLMDRVRNNF-RIPQPNGLKISITA 70 Query: 236 VGEELHEIGLRMLADFFEMEGWNTCYLGSNMPAEGLIRYLKEQPADLLAVSVTMAYHVSE 295 + ++ H +G M ADFF +GWN LGS++P + L++Y+K D + +S T + Sbjct: 71 IEDDKHVMGTYMSADFFRWDGWNVELLGSSIPNDDLLKYIKSSLPDFVLLSST---YPKS 127 Query: 296 VQRLIASIRTHAGLDR-IKILVGG---MPFNIDRTLWEKVGADGYAPDAKGALEVASQLI 351 ++LI SI L++ IKILVGG + D+ L DG+A D A +A+ L+ Sbjct: 128 TEKLIESIHNIRSLEKNIKILVGGPGAISLKDDKKL-----IDGFAEDPLKATYLANSLV 182 Query: 352 SQN 354 N Sbjct: 183 DSN 185 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 508,397,266 Number of extensions: 20576568 Number of successful extensions: 63197 Number of sequences better than 1.0e-05: 86 Number of HSP's gapped: 63179 Number of HSP's successfully gapped: 86 Length of query: 354 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 227 Effective length of database: 412,420,005 Effective search space: 93619341135 Effective search space used: 93619341135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)