BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0086.p (658 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_616281.1| FmtA-like protein [Methanosarcina acetivorans s... 223 1e-56 gb|EAI75476.1| unknown [environmental sequence] 182 2e-44 ref|NP_420908.1| conserved hypothetical protein [Caulobacter cre... 148 5e-34 ref|NP_125831.1| hypothetical beta-lactamase precursor (penicill... 142 2e-32 ref|NP_560593.1| beta-lactamase-like protein [Pyrobaculum aeroph... 139 3e-31 >ref|NP_616281.1| FmtA-like protein [Methanosarcina acetivorans str. C2A] gb|AAM04761.1| FmtA-like protein [Methanosarcina acetivorans str. C2A] Length = 671 Score = 223 bits (568), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 150/415 (36%), Positives = 216/415 (52%), Gaps = 19/415 (4%) Query: 27 NAS-PVNLGKAEIEAFANTFFQQKEVKDQLAGAVLVVVKNGQVLLNKGYGYADVGSKKPF 85 NAS P N AE+EAF + + + + GA + VVK+GQ++ KGYGYAD+ ++P Sbjct: 59 NASGPTN--SAELEAFMDGAVNAQLKANHIPGATVAVVKDGQIIFAKGYGYADIDKRQPV 116 Query: 86 DSDHTVFRVASVSKLFTAVGVMQLAEKGKLDLDKDVQAYLPDVQIPNKTGGPLTLKHLMT 145 + T+FRV SVSKLF VMQLAE+GKLDLD DV YL D Q+P P+TLK+LM+ Sbjct: 117 VGNQTLFRVGSVSKLFVWTAVMQLAEQGKLDLDADVNTYLKDFQVPATYSKPITLKNLMS 176 Query: 146 HTGGFD----AGT--AEDPQKTYSLRDFIKETVPTVITKPGEAFNYNNYGYDLQGYIIEK 199 HT GF+ +G + L +++K+ +P + PGE Y+NYG L YI+E+ Sbjct: 177 HTSGFEDLAISGRFFVHNSTDIMPLEEYLKKGMPARVRPPGELTAYSNYGSALAAYIVEQ 236 Query: 200 VSGLPFEEYMMKNLFAPLGMNNSDFI--FTDKVKKAIATPYD-SSLKQAPQPVNVPVNRP 256 VSG+PF+ Y+ +N+ PL MNN+ F ++ ++ Y S+ +P P Sbjct: 237 VSGMPFDRYVEENILLPLDMNNTTFDQPLPARLASNMSNGYVYSNNAYTAKPFEYVQVWP 296 Query: 257 AGGMFSTGADMAKFLMAMLNDDPASKPRILTEASIRAMEHNSVTIHPKIPGAGYG-LESN 315 A M ST DMAKF++A L + RIL EA+ + M T P + G YG LE N Sbjct: 297 ASSMSSTSEDMAKFMIAHLQNGRYGDKRILQEATAQQMHSRLFTNAPGVNGVTYGFLEIN 356 Query: 316 YTKYYNGYNVVEKGGDLPGFHSNIWLLPDEKTGVFLAFN-SDKGNLRLPFFEQFMNRYFP 374 V+ GD FH+ + L+P+ + G+F++ N D + + FM+ Y+P Sbjct: 357 ----PENPRVIGHEGDTILFHTQLALIPESQLGLFISLNEEDSESAAFELLQAFMDHYYP 412 Query: 375 KKGTGPAFVQPEPTKQQLLRFEGTYRDLRSPGWSYDISAVDGALIVKAPSGNHTL 429 P K L F G YR RS +++ I +P N TL Sbjct: 413 TPPPPVPEPIPGFGKNASL-FAGNYRPTRSAYTNFEKLTSISQRIRISPGSNSTL 466 >gb|EAI75476.1| unknown [environmental sequence] Length = 403 Score = 182 bits (462), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 124/386 (32%), Positives = 191/386 (49%), Gaps = 23/386 (5%) Query: 32 NLGKAEIEAFANTFFQQKEVKDQLAGAVLVVVKNGQVLLNKGYGYADVGSKKPFDSDHTV 91 + +AE E F + Q K L+ + +V N Q+++++GYG+AD + + D + Sbjct: 12 QVAQAEFEQFLDELIQPHLEKAALSNVSVALVYNNQLVVSRGYGFADQLNGQKLDPSLHL 71 Query: 92 FRVASVSKLFTAVGVMQLAEKGKLDLDKDVQAYLPDVQIPNKTGGPLTLKHLMTHTGGFD 151 R SVSKL T VMQL E+G +DLD D+ YL D IP+ GP+T++HLMTH GF+ Sbjct: 72 IRPGSVSKLLTWTAVMQLVEQGLIDLDADINNYL-DFTIPSHPSGPVTMRHLMTHRAGFE 130 Query: 152 AG----TAEDPQKTYSLRDFIKETVPTVITKPGEAFNYNNYGYDLQGYIIEKVSGLPFEE 207 A P++ S +++K VP + G Y+NYG L GYI+E+VS +PFE Sbjct: 131 DVLRDLMATSPEQLVSNEEWLKRWVPQRVFAAGTVPAYSNYGAALAGYIVERVSKVPFEH 190 Query: 208 YMMKNLFAPLGMNNSDFI--FTDKVKKAIATPYD-SSLKQAPQPVNVPVNRPAGGMFSTG 264 Y+ +++F PLGM NS F +D + IA Y+ S +Q P P+ PAG + +T Sbjct: 191 YIEQHIFQPLGMKNSSFRQPVSDALAGQIAKIYNGSGTEQVPYDYISPI--PAGSLSTTA 248 Query: 265 ADMAKFLMAMLNDDPASKPRILTEASIRAMEHNSVTIHPKIPGAGYGLESNYTKYYNGYN 324 DM F+ A L+D + P +L+ S M S +P G + Sbjct: 249 QDMGLFMAAFLDD---AAP-LLSAKSRDLMLSYSAPHVDGVPSMSLGFIRADEQ---AQQ 301 Query: 325 VVEKGGDLPGFHSNIWLLPDEKTGVFLAFNSDKGN-----LRLPFFEQFMNRYFPKKGTG 379 + G+ +HS++ L+P+ G+F++ S G LR F+ +Y P K Sbjct: 302 IWGHSGNTRTYHSHLMLIPEVGAGLFVSIGSAAGRAQAYELRQALNTAFIKKYLPAKAIE 361 Query: 380 PAF-VQPEPTKQQLLRFEGTYRDLRS 404 P KQ+ G Y++ R+ Sbjct: 362 TLLHSDPAGAKQRADEVAGYYQNSRT 387 >ref|NP_420908.1| conserved hypothetical protein [Caulobacter crescentus CB15] pir||H87509 conserved hypothetical protein CC2105 [imported] - Caulobacter crescentus gb|AAK24076.1| conserved hypothetical protein [Caulobacter crescentus CB15] Length = 712 Score = 148 bits (373), Expect = 5e-34, Method: Compositional matrix adjust. Identities = 105/348 (30%), Positives = 169/348 (48%), Gaps = 25/348 (7%) Query: 33 LGKAEIEAFANTFFQQKEVKDQLAGAVLVVVKNGQVLLNKGYGYADVGSKKPFDSDHTVF 92 L + ++EAF + Q+ +D +AG L VV+NGQV+L KGYG++++G +K D D T+F Sbjct: 94 LPRPDLEAFVDGVVQRAMTRDHIAGVTLSVVQNGQVVLKKGYGFSNLGQRKQVDPDKTLF 153 Query: 93 RVASVSKLFTAVGVMQLAEKGKLDLDKDVQAYLPD-VQIPNK-TGGPLTLKHLMTHTGGF 150 RVAS+SK FT + +M E G++ LD V YLP+ +Q+ ++ + L+ LMTHT GF Sbjct: 154 RVASISKTFTWITLMNEIEAGRMRLDGPVNLYLPEPLQVKDQGKKTQIRLRDLMTHTSGF 213 Query: 151 DAGT-----AEDPQKTYSLRDFIKETVPTVITKPGEAFNYNNYGYDLQGYIIEKVSGLPF 205 + DP + L D++++ P + +PG Y+NYG L G + V+G PF Sbjct: 214 EDRALGQLFERDPNRVRPLYDYLRQERPRRVREPGLMPTYSNYGAALAGAAVSNVTGKPF 273 Query: 206 EEYMMKNLFAPLGMNNSDFIFTDKVKKAIATPYDSSL---------------KQAPQPVN 250 E+ + + + P G+ + F + + P +L K P+ Sbjct: 274 EQLVSEEIIIPAGLTRTTFREARPWRDDLPAPMAETLAADLSDAFSWTAMGWKTQPREFM 333 Query: 251 VPVNRPAGGMFSTGADMAKFLMAMLNDDPASKPRILTEASIRAMEHNSVTIHPKIPGAGY 310 V A ST +DMA+++ +LN I + + +A S P AG+ Sbjct: 334 GQVAP-AASASSTASDMARYMTLLLNGGTIDGKTIFSPRTAQAFR--SPMYRPAPDAAGW 390 Query: 311 GLESNYTKYYNGYNVVEKGGDLPGFHSNIWLLPDEKTGVFLAFNSDKG 358 G G FHSN+ ++PD G+F++ N+D G Sbjct: 391 NAGFQDVPLPGGRRGFGHQGATLYFHSNLVIVPDLNLGIFVSVNTDSG 438 >ref|NP_125831.1| hypothetical beta-lactamase precursor (penicillin-binding protein) [Pyrococcus abyssi] pir||G75201 probable beta-lactamase (EC 3.5.2.6) PAB0087 - Pyrococcus abyssi (strain Orsay) emb|CAB49062.1| pbp related beta-lactamase precursor (EC 3.5.2.6) (penicillin-binding protein) [Pyrococcus abyssi] Length = 447 Score = 142 bits (359), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 108/347 (31%), Positives = 179/347 (51%), Gaps = 40/347 (11%) Query: 31 VNLGKAEIEAFANTFFQQKEVKDQLAGAVLVVVKNGQVLLNKGYGYADVGSKKPFDSDHT 90 +++GK E +F +K + ++ G + ++K+G V+ KG+GY +V ++ P + T Sbjct: 1 MDVGKLE------SFIVEKMAERKVPGISISIIKDGDVVYAKGFGYRNVEARLP-STPET 53 Query: 91 VFRVASVSKLFTAVGVMQLAEKGKLDLDKDVQAYLPDVQIPNKTGGPLTLKHLMTHTGGF 150 ++ + S++K FTA+ +M+L E+G L LD V+ ++ P G P+T+ HL+TH+ G Sbjct: 54 IYGIGSITKSFTALAIMKLVEEGGLSLDDPVEKFVNIKLRP--FGEPVTVHHLLTHSSGI 111 Query: 151 -DAGTAE----------------DPQKTYSLRDFIKETVPTVITKPGEAFNYNNYGYDLQ 193 G AE P++T + F ++ + KPGE F Y N GY L Sbjct: 112 PSLGYAEAFIDGMVGGDNWLPVSTPEETIA---FARDMEKWAVAKPGERFFYLNTGYVLL 168 Query: 194 GYIIEKVSGLPFEEYMMKNLFAPLGMNNSDFIFTDKVKK----AIATPYDSSLKQAPQPV 249 G IIEKVSG+ +EEY+ K + PLGMN S F F ++V+K A+ D + PQP Sbjct: 169 GKIIEKVSGVSYEEYIKKKILEPLGMNRSYF-FKEEVEKDKDVAMGYILDKEGRLVPQPF 227 Query: 250 NVPVNRPAGGMFSTGADMAKFLMAMLNDDPASKPRILTEASIRAMEHNSVTIHPKI-PGA 308 + GG+ S+ D+AK+L + D + I+++ I ME + + + +I G Sbjct: 228 PYGIT-ADGGLLSSVLDLAKYLKMYIERDES----IVSKEYIEKMETSYIKVPWEIFGGE 282 Query: 309 GYGLESNYTKYYNGYNVVEKGGDLPGFHSNIWLLPDEKTGVFLAFNS 355 GYG + G +V G + + I +P++K GV + NS Sbjct: 283 GYGYGLIIYPNFLGEKLVGHSGSVGMYTGYIGYIPEKKIGVAVLENS 329 >ref|NP_560593.1| beta-lactamase-like protein [Pyrobaculum aerophilum] gb|AAL64775.1| beta-lactamase-like protein [Pyrobaculum aerophilum str. IM2] Length = 448 Score = 139 bits (349), Expect = 3e-31, Method: Compositional matrix adjust. Identities = 117/420 (27%), Positives = 204/420 (48%), Gaps = 38/420 (9%) Query: 43 NTFFQQKEVKDQLAGAVLVVVKNGQVLLNKGYGYADVGSKKPFDSDHTVFRVASVSKLFT 102 + F +K +L V+K+G+V G+ D+ P + T++ + S++K FT Sbjct: 5 DAFILEKIAATKLPSVSYAVIKDGEVAYANALGFRDLERGLP-ATPATIYGIGSITKSFT 63 Query: 103 AVGVMQLAEKGKLDLDKDVQAYLPDVQIPNKTGGPLTLKHLMTHTGGFDA---------- 152 A+ +++L E+G+L L+ +V+ Y+P +++ ++ G +T++HL+THT G A Sbjct: 64 ALCILKLVEEGRLSLNDNVEKYIP-IRLRSR-GENVTIRHLLTHTSGIPALGYAEAFIEG 121 Query: 153 -----GTAEDPQKTYSLRDFIKETVPTVITKPGEAFNYNNYGYDLQGYIIEKVSGLPFEE 207 GT K L F++ + KPGE F Y N GY L G +IEKV+G P+ E Sbjct: 122 LVSRGGTWLPVAKPQDLLPFMESAEEWAVAKPGERFFYLNEGYVLLGLVIEKVTGAPYWE 181 Query: 208 YMMKNLFAPLGMNNSDFIFTDKVKK--AIATP-YDSSLKQAPQPVNVPVNRPA-GGMFST 263 + + PLGM + ++ +++V K +A P Y K P P +P+ + GG+ S Sbjct: 182 CVRMKILTPLGMLRT-YLASEEVMKDGDVAKPYYPDPEKGTPTPGRIPIGITSDGGIMSN 240 Query: 264 GADMAKFLMAMLNDDPASKPRILTEASIRAMEHNSVTIHPKIPG-AGYGLESNYTKYYNG 322 DMAK++ ++N + I+ + S+ E V + ++ G GYG ++ + G Sbjct: 241 ALDMAKYVAMLINRGTYNGVEIIGKKSVEEAEIKRVAVPWRLLGDEGYGYGLVISENFYG 300 Query: 323 YNVVEKGGDLPGFHSNIWLLPDEKTGVFLAFNSDKGNLRLPFFEQFMNRYFPKKGTGPAF 382 +V GG++ + +++ LP ++ GV + NS P M G P Sbjct: 301 RKIVMHGGNVLVYTAHMAYLPYDRIGVVVLANSTG----YPMSYIAMYILAIALGRNPEE 356 Query: 383 VQPEPTKQQLLR-FEGTYRDLRSPGWSYDISAVDGALIVKAPSGNHTLRQAEDLLFYDEE 441 + P ++++LR EGTYR + +Y A LI+K+ G E+L + EE Sbjct: 357 L-PFLMREKVLRKLEGTYRGYKGT-VTYTAKAKGDILILKSQWG-------EELYLFPEE 407 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,033,493,592 Number of extensions: 46906520 Number of successful extensions: 95077 Number of sequences better than 1.0e-05: 705 Number of HSP's gapped: 94408 Number of HSP's successfully gapped: 706 Length of query: 658 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 526 Effective length of database: 398,844,510 Effective search space: 209792212260 Effective search space used: 209792212260 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 129 (54.3 bits)