BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0085.p (707 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00080135.1| COG2200: FOG: EAL domain [Geobacter metallire... 384 e-105 ref|ZP_00060071.1| COG2200: FOG: EAL domain [Clostridium thermoc... 381 e-104 ref|NP_953093.1| sensory box/GGDEF family protein [Geobacter sul... 377 e-103 ref|NP_952000.1| sensory box/GGDEF family protein [Geobacter sul... 367 e-100 ref|NP_385065.1| HYPOTHETICAL PROTEIN [Sinorhizobium meliloti] >... 362 1e-98 >ref|ZP_00080135.1| COG2200: FOG: EAL domain [Geobacter metallireducens] Length = 633 Score = 384 bits (986), Expect = e-105, Method: Compositional matrix adjust. Identities = 198/427 (46%), Positives = 275/427 (64%), Gaps = 5/427 (1%) Query: 271 RINYLAYHDDLTGLPNRRRLMQRVEEMLLQSERDKSRGFTALVIMNINHFKNINDSLGHY 330 RI +AY+D LTGLPNRR R+ + L ++R K A++ ++++ FK IND+LGH Sbjct: 191 RIRQMAYYDSLTGLPNRRLFNDRLRQSLALAQRHKRP--LAVMFLDLDRFKLINDTLGHA 248 Query: 331 AGDLLLQLVAKRIGNEAK-ANEELYSMGADEFAFLMTDRTGVENCLIRAGELLRLFDTPV 389 GD LL VAKR+ N + + + G DEF ++ + V++ A +++ +F TP Sbjct: 249 VGDRLLMAVAKRLRNCCRREGDTVARQGGDEFIINLSAISDVKDAFTVAQKIIDVFRTPF 308 Query: 390 QLESGEYHISLSLGMSIYPGDGDTAEQLIQNADTAVHNAKDQGVE-IRRYIPAMQMKAKE 448 ++ E I+ S+G+S+YP DG + + L++NAD A++ AK+QG + + P M +A E Sbjct: 309 VIDGYELFITTSIGISVYPHDGRSGDALVKNADVAMYRAKEQGRNNCQLFAPEMNARAFE 368 Query: 449 RLKLENDLRRALERDEFYLVYQPQVQLETEEIVGMEALLRWNHPKRGLVSPVDFIPIAEE 508 RL LEND+RRALERDEF L YQP+V +E +I MEAL+RW HP GLV P FIP+AEE Sbjct: 369 RLSLENDMRRALERDEFVLHYQPKVNVEHGQIACMEALVRWQHPSMGLVPPSRFIPLAEE 428 Query: 509 SGLIVPIGEWVLKTACLQNKMWQNAGYRPICVSINLSMRQFLQPNLAGKIDVILQDIGLD 568 +GLIVP+GEWVL+TAC QN+ WQ+AGY + V++NLS RQ Q +L+ + +L + GL Sbjct: 429 TGLIVPLGEWVLRTACQQNRAWQDAGYPTMQVAVNLSPRQLRQGDLSETVARVLAETGLA 488 Query: 569 PCYVDLEITESMTLDK-ETAFDQLNRLKRLGVFISIDDFGTGYSSLHYLKNMPIDRLKID 627 P ++ +E+TES+ +D E RL R+GV ISIDDFGTGYSSLHYLK P+ LKID Sbjct: 489 PGWLVIEVTESILMDDAEETIRTFRRLDRMGVRISIDDFGTGYSSLHYLKKFPVHTLKID 548 Query: 628 RSFVSEVLEDSNNAAIVSTITSMAHHLKLKVTAEGVENXXXXXXXXXXXCHEGQGYLFSK 687 RSFV E+ + ++ AI + SMA L L V AEGVE C QG+LFS+ Sbjct: 549 RSFVREIASNPDDEAIAGAVISMAKGLNLGVVAEGVETVEQMEVLRALNCRYMQGFLFSE 608 Query: 688 PIKAAEF 694 P A +F Sbjct: 609 PAPAEQF 615 >ref|ZP_00060071.1| COG2200: FOG: EAL domain [Clostridium thermocellum ATCC 27405] Length = 862 Score = 381 bits (979), Expect = e-104, Method: Compositional matrix adjust. Identities = 194/423 (45%), Positives = 272/423 (64%), Gaps = 4/423 (0%) Query: 275 LAYHDDLTGLPNRRRLMQRVEEMLLQSERDKSRGFTALVIMNINHFKNINDSLGHYAGDL 334 + Y+D LTGLPNR R++ L QS+R+ AL+I+++++FK IND+LGH GD+ Sbjct: 425 MEYYDPLTGLPNRSLFSDRLKMALAQSKRNNE--MCALIILDLDNFKAINDTLGHSVGDM 482 Query: 335 LLQLVAKRIGNEAKANEELYSMGADEFAFLMTDRTGVENCLIRAGELLRLFDTPVQLESG 394 LL+ VA++I + + + +G DEF + + + A +L+ F P LE Sbjct: 483 LLKQVAEKIKGYLREVDTVARIGGDEFIIIQPQIKDINDATRAADRILKKFQQPWILEGK 542 Query: 395 EYHISLSLGMSIYPGDGDTAEQLIQNADTAVHNAKDQGVE-IRRYIPAMQMKAKERLKLE 453 EY+I+ S+G+ IYP G+ + + +NADTA++ AK+ G + Y +M K +RL +E Sbjct: 543 EYYITASMGIGIYPNGGEDPQTIFKNADTALYRAKELGRNNYQLYTESMNQKVLQRLDIE 602 Query: 454 NDLRRALERDEFYLVYQPQVQLETEEIVGMEALLRWNHPKRGLVSPVDFIPIAEESGLIV 513 N LRRA+E++EF L YQPQ+ ++T +IVG EALLRW HP GL+ P++FIP+AE+SGLIV Sbjct: 603 NSLRRAIEKEEFVLFYQPQIDIKTGKIVGFEALLRWYHPDYGLMPPMEFIPVAEDSGLIV 662 Query: 514 PIGEWVLKTACLQNKMWQNAGYRPICVSINLSMRQFLQPNLAGKIDVILQDIGLDPCYVD 573 IGEWVL+TAC QNK W G P +S+NLS RQF + N+ ID I GL P ++ Sbjct: 663 VIGEWVLETACRQNKKWIECGLEPHLISVNLSARQFQRSNIVEVIDRIRSSTGLAPELLE 722 Query: 574 LEITESMTL-DKETAFDQLNRLKRLGVFISIDDFGTGYSSLHYLKNMPIDRLKIDRSFVS 632 LEITES + D D LN+L++ G+ +S+DDFGTGYSSL+YL+ +PID LKID+SFV Sbjct: 723 LEITESTAMQDLSFTIDVLNQLRKKGIRVSLDDFGTGYSSLNYLRQLPIDTLKIDKSFVQ 782 Query: 633 EVLEDSNNAAIVSTITSMAHHLKLKVTAEGVENXXXXXXXXXXXCHEGQGYLFSKPIKAA 692 ++ +S AI T+ S+AH L L V AEGVE C + QGYLFSKP+ A Sbjct: 783 DIRANSKEEAIAKTVISLAHKLDLTVVAEGVETKEQLLFLKKEKCDKAQGYLFSKPLPAE 842 Query: 693 EFE 695 E E Sbjct: 843 EIE 845 >ref|NP_953093.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] gb|AAR35420.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] Length = 762 Score = 377 bits (968), Expect = e-103, Method: Compositional matrix adjust. Identities = 195/430 (45%), Positives = 276/430 (64%), Gaps = 5/430 (1%) Query: 272 INYLAYHDDLTGLPNRRRLMQRVEEMLLQSERDKSRGFTALVIMNINHFKNINDSLGHYA 331 I +AY+D LTGLPNRR R+ + L ++R+K +++ ++++ FK IND+LGH Sbjct: 321 IRQMAYYDSLTGLPNRRLFNDRLRQSLALAQRNKR--LLSVLFLDLDRFKLINDTLGHAV 378 Query: 332 GDLLLQLVAKRI-GNEAKANEELYSMGADEFAFLMTDRTGVENCLIRAGELLRLFDTPVQ 390 GD LL VAKR+ G + + + G DEF ++ + V++ L A +++ TP Sbjct: 379 GDKLLMQVAKRLKGVCRRDGDTVARQGGDEFIINLSVISDVKDALKVAQKIIDSLRTPFV 438 Query: 391 LESGEYHISLSLGMSIYPGDGDTAEQLIQNADTAVHNAKDQGVE-IRRYIPAMQMKAKER 449 ++ E I+ S+G+S++P DG T++ L++NAD A++ AK+QG + Y P M KA ER Sbjct: 439 IDGHELFITTSIGISVFPYDGRTSDALVKNADFAMYRAKEQGRNNCQLYTPEMNSKAFER 498 Query: 450 LKLENDLRRALERDEFYLVYQPQVQLETEEIVGMEALLRWNHPKRGLVSPVDFIPIAEES 509 L LEN+LR+ LER+E L YQP+V +E +I MEAL+RW HP GLV P FIP+AEE+ Sbjct: 499 LSLENNLRKGLEREELVLYYQPKVNVEDGQISCMEALVRWQHPTMGLVPPARFIPLAEET 558 Query: 510 GLIVPIGEWVLKTACLQNKMWQNAGYRPICVSINLSMRQFLQPNLAGKIDVILQDIGLDP 569 GLIVP+GEWVL+TAC QN+ WQ AGY P+ V++N+S RQ Q NL ++ L D GL P Sbjct: 559 GLIVPLGEWVLRTACRQNREWQKAGYPPMQVAVNISPRQLQQGNLVDVVERALSDSGLAP 618 Query: 570 CYVDLEITESMTLD-KETAFDQLNRLKRLGVFISIDDFGTGYSSLHYLKNMPIDRLKIDR 628 ++ LE+TES+ +D E+ L+R+G+ I+IDDFGTGYSSLHYLK PI LKIDR Sbjct: 619 RWLVLEVTESIMMDGTESTLATFRVLERMGIHIAIDDFGTGYSSLHYLKKFPIHALKIDR 678 Query: 629 SFVSEVLEDSNNAAIVSTITSMAHHLKLKVTAEGVENXXXXXXXXXXXCHEGQGYLFSKP 688 SF+ ++ + ++ AI S + +MA L LKV AEGVE C QG+LFS+P Sbjct: 679 SFIRDITTNPDDEAITSAVITMAKGLNLKVVAEGVETVEQLEVLRALQCTHMQGHLFSRP 738 Query: 689 IKAAEFETFF 698 A +F F Sbjct: 739 APAEQFAPVF 748 >ref|NP_952000.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] gb|AAR34273.1| sensory box/GGDEF family protein [Geobacter sulfurreducens PCA] Length = 842 Score = 367 bits (941), Expect = e-100, Method: Compositional matrix adjust. Identities = 195/431 (45%), Positives = 265/431 (61%), Gaps = 6/431 (1%) Query: 272 INYLAYHDDLTGLPNRRRLMQRVEEMLLQSERDKSRGFTALVIMNINHFKNINDSLGHYA 331 I LAY D LTGLPNR RV L + R++ G AL+ ++++ FK+IND+LGH Sbjct: 395 IRRLAYFDPLTGLPNRALFQDRVSHALAVARREE--GAVALLFLDLDRFKDINDTLGHAM 452 Query: 332 GDLLLQLVAKRIGNEAKANEELYSMGADEFAFLMTDRTGVENCLIRAGELLRLFDTPVQL 391 GDLLL VA R+ + ++ L +G DEF ++T I A LL L L Sbjct: 453 GDLLLTSVADRLKAHVRVSDTLARLGDDEFVVILTGARDERGVSIVAQSLLDLLTPSFDL 512 Query: 392 ESGEYHISLSLGMSIYPGDGDTAEQLIQNADTAVHNAKDQGVEIRR-YIPAMQMKAKERL 450 E E IS S+G++++P DG+ L+ AD A+H AK+ G + R Y M A++R Sbjct: 513 EGKEVFISTSIGIALFPSDGEDGNVLLSRADMALHAAKEAGRNVYRFYSDDMNRSAQDRR 572 Query: 451 KLENDLRRALERDEFYLVYQPQVQLETEEIVGMEALLRWNHPKRGLVSPVDFIPIAEESG 510 LE+ LRRAL +EFYL YQPQ+ + T I G+EALLRWNHP+ GL+ P FIP AEE G Sbjct: 573 GLEDRLRRALANEEFYLDYQPQIDMRTGGIFGVEALLRWNHPEEGLIPPGRFIPAAEEMG 632 Query: 511 LIVPIGEWVLKTACLQNKMWQNAGYRPICVSINLSMRQFLQPNLAGKIDVILQDIGLDPC 570 I IGEWVL+ ACLQ + WQ +G P+ V++N+S QF QP +D +L++ GLDP Sbjct: 633 FIRRIGEWVLRAACLQCRAWQESGLPPVRVAVNVSGHQFNQPGFIDMVDRVLEETGLDPT 692 Query: 571 YVDLEITESMTLD--KETAFDQLNRLKRLGVFISIDDFGTGYSSLHYLKNMPIDRLKIDR 628 ++LE+TES L+ ++T ++ LK G+ ++IDDFGTGYSSL YLK+ PIDRLKIDR Sbjct: 693 LLELELTESTLLEGARDTIMTLID-LKVRGIHLAIDDFGTGYSSLSYLKHFPIDRLKIDR 751 Query: 629 SFVSEVLEDSNNAAIVSTITSMAHHLKLKVTAEGVENXXXXXXXXXXXCHEGQGYLFSKP 688 SFV +++ D ++ AIV I +MAH L L+V AEGVE C E QGY F +P Sbjct: 752 SFVRDIVSDLDDRAIVEAIIAMAHSLGLRVLAEGVEREEELELLRERGCDEVQGYYFGRP 811 Query: 689 IKAAEFETFFL 699 + A + E + Sbjct: 812 MSARDLEARLM 822 >ref|NP_385065.1| HYPOTHETICAL PROTEIN [Sinorhizobium meliloti] emb|CAC45531.1| HYPOTHETICAL PROTEIN [Sinorhizobium meliloti] Length = 772 Score = 362 bits (930), Expect = 1e-98, Method: Compositional matrix adjust. Identities = 189/432 (43%), Positives = 275/432 (63%), Gaps = 7/432 (1%) Query: 271 RINYLAYHDDLTGLPNRRRLMQRVEEMLLQ-SERDKSRGFTALVIMNINHFKNINDSLGH 329 RI ++A+HD LTGLPNR ++V ML + ERD+ + L ++++ FK INDSLGH Sbjct: 339 RIGFMAHHDALTGLPNRVLFEEQVAGMLEEIRERDQ---WAVLAFLDLDDFKLINDSLGH 395 Query: 330 YAGDLLLQLVAKRIGNEAKANEELYSMGADEFAFLMTDRTGVENCLIRAGELLRL-FDTP 388 GD LL++VA R+ + ++ + +G DEF L+ + ++ E +R P Sbjct: 396 ATGDELLKIVAGRMRAAVRRSDSVVRVGGDEFIILLNGLPKERDVVLSRLEDIRTAIAMP 455 Query: 389 VQLESGEYHISLSLGMSIYPGDGDTAEQLIQNADTAVHNAKDQGVE-IRRYIPAMQMKAK 447 VQL+ +S S+G++ +P G TA +L+ NAD A++ AK+ G I+ + M +A Sbjct: 456 VQLQGRSLQVSCSMGVACFPNQGKTAGELLANADMAMYRAKELGRNNIQVFTEEMAARAH 515 Query: 448 ERLKLENDLRRALERDEFYLVYQPQVQLETEEIVGMEALLRWNHPKRGLVSPVDFIPIAE 507 E+L+ + +LR AL R+EF L +QPQ+ LET I EALLRW HP RG++SP FIP+AE Sbjct: 516 EKLQSQEELREALAREEFLLHFQPQMSLETGRIFAAEALLRWQHPVRGIISPAAFIPLAE 575 Query: 508 ESGLIVPIGEWVLKTACLQNKMWQNAGYRPICVSINLSMRQFLQPNLAGKIDVILQDIGL 567 E+GLIVPIG+WVL+TAC Q K WQ+AG P+ VS+N+S RQF + N A ++ +L++ L Sbjct: 576 ETGLIVPIGDWVLRTACRQLKAWQDAGLPPLIVSVNVSARQFRERNWAARVAAVLEETCL 635 Query: 568 DPCYVDLEITESMTL-DKETAFDQLNRLKRLGVFISIDDFGTGYSSLHYLKNMPIDRLKI 626 + Y++LE+TES+ + D A ++ L+ +GV ++IDDFGTGYSSL LK P+ RLKI Sbjct: 636 EARYLELELTESLIMQDVPGAIATMHALEAIGVHLAIDDFGTGYSSLSALKRFPVRRLKI 695 Query: 627 DRSFVSEVLEDSNNAAIVSTITSMAHHLKLKVTAEGVENXXXXXXXXXXXCHEGQGYLFS 686 DRSFV+++ D+++ AI S I S+A L L+V AEGVE C E QGY FS Sbjct: 696 DRSFVTDIPHDADDMAITSAIISLAQKLGLRVIAEGVETEAQVEFLQKSGCGEIQGYFFS 755 Query: 687 KPIKAAEFETFF 698 +P+ +FE Sbjct: 756 QPLPGEDFEVLL 767 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.139 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 916,984,699 Number of extensions: 35786122 Number of successful extensions: 82803 Number of sequences better than 1.0e-05: 2503 Number of HSP's gapped: 78534 Number of HSP's successfully gapped: 2507 Length of query: 707 Length of database: 757,237,578 Length adjustment: 133 Effective length of query: 574 Effective length of database: 396,129,411 Effective search space: 227378281914 Effective search space used: 227378281914 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)