BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0079.p (514 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAP73418.1| thermostable leucine aminopeptidase [Geobacillus ... 347 4e-94 ref|NP_391085.1| similar to leucyl aminopeptidase [Bacillus subt... 342 1e-92 ref|NP_653388.1| Peptidase_M17, Cytosol aminopeptidase family, c... 325 2e-87 ref|NP_981353.1| cytosol aminopeptidase [Bacillus cereus ATCC 10... 323 7e-87 ref|NP_834593.1| Cytosol aminopeptidase [Bacillus cereus ATCC 14... 322 2e-86 >gb|AAP73418.1| thermostable leucine aminopeptidase [Geobacillus kaustophilus] Length = 497 Score = 347 bits (890), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 189/381 (49%), Positives = 249/381 (65%), Gaps = 9/381 (2%) Query: 120 AVRPHEAGQALTEGLILGAYRRTHYK--REQPVYAGLESAVFHLDRQAEAAESLEWSQGI 177 AV +EA AL E L Y YK + QP Y LES + + +A + Sbjct: 119 AVDGNEAAHALAEAYHLATYEFPGYKQKKSQPDYQ-LESLIVYTAAEAAEI-----EASL 172 Query: 178 RQGQAFGEATNLARXXXXXXXXXXXXSALAMAAIEVAERHGFPAEVLDEQEIAQKGMGGL 237 G +G+ATN AR S LA A+++A R+ F EVL+++++ + GMG L Sbjct: 173 FVGSVYGKATNSARTLVNTPGNLLTASDLADYAVKLANRYDFDYEVLEKEDMERLGMGAL 232 Query: 238 LAVGKGSVHPPRMIVIRYQGTSQWENVTAIVGKGITFDSGGISLKRAPGMEDMISDMGGA 297 LAV +GS PP++IV++YQG +WE+V A+VGKG+TFD+GG LK M DM +DM GA Sbjct: 233 LAVNQGSKQPPKLIVLKYQGKDEWEDVIALVGKGVTFDTGGYCLKPRDSMVDMKTDMAGA 292 Query: 298 AAVLGVMEALGTLRPRINIVMLIPSAENMPAANAFKPGDIVTTLSGRTIEILNTDAEGRV 357 AAVLG MEA+G LRP N++ +IP+ +NM + AFKP D++T+LSG+TIE+ NTDAEGR+ Sbjct: 293 AAVLGAMEAIGELRPEQNVLAVIPATDNMISGEAFKPDDVITSLSGKTIEVKNTDAEGRL 352 Query: 358 VLADALTYSLEWGAERIIDVATLTGAVLSVLGDIATGAVTNNEVMMEALQTASIRAGEKI 417 +LADA+TY+ + GA IIDVATLTG V+ LG TGA+TNNE + E L AS+ GE I Sbjct: 353 ILADAVTYAKQHGASYIIDVATLTGGVIVALGTDKTGAMTNNEELFEQLLEASMETGEFI 412 Query: 418 WRLPVFPEFREML-SSEVADIRNAAGRYGGASTAGLFIGEFAEGRPWVHLDIAGTAFLSK 476 WRLP+ RE + SS++AD+ N+ GR G A G FIGEFAE PWVHLDIAGTA K Sbjct: 413 WRLPITENDRERVRSSKIADLNNSPGREGHAIMGGAFIGEFAEDTPWVHLDIAGTAATKK 472 Query: 477 ERGVNPKGATGVMVRTLLQYL 497 + + P GATGVMVRTL ++ Sbjct: 473 DGDLGPAGATGVMVRTLTAFV 493 >ref|NP_391085.1| similar to leucyl aminopeptidase [Bacillus subtilis] sp|O32106|AMPA_BACSU Probable cytosol aminopeptidase (Leucine aminopeptidase) (LAP) (Leucyl aminopeptidase) pir||F70012 leucyl aminopeptidase homolog yuiE - Bacillus subtilis emb|CAB15195.1| yuiE [Bacillus subtilis subsp. subtilis str. 168] Length = 500 Score = 342 bits (877), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 180/382 (47%), Positives = 246/382 (64%), Gaps = 21/382 (5%) Query: 121 VRPHEAGQALTEGLILGAYRRTHYKRE--QPVYAGLESAVFHLDRQAEAA------ESLE 172 V P +A AL E +L +Y YK + +P D+Q EA ++ E Sbjct: 116 VPPADAAHALAESCLLASYEVQDYKHKSNEP------------DKQIEAVYVVTDEDTQE 163 Query: 173 WSQGIRQGQAFGEATNLARXXXXXXXXXXXXSALAMAAIEVAERHGFPAEVLDEQEIAQK 232 G+R GQA+G+ TN AR + LA A E+A ++ F E+L++ E+ + Sbjct: 164 VQAGLRVGQAYGQGTNSARTLVNMPGNMLTATDLASYAEELAAKYDFECEILEKSEMEEL 223 Query: 233 GMGGLLAVGKGSVHPPRMIVIRYQGTSQWENVTAIVGKGITFDSGGISLKRAPGMEDMIS 292 GMGG+LAV +GS PP+MIV++YQG +WE+V +VGKGITFD+GG S+K G+ M S Sbjct: 224 GMGGILAVNQGSTEPPKMIVLKYQGKKEWEDVVGLVGKGITFDTGGYSIKTKSGIVGMKS 283 Query: 293 DMGGAAAVLGVMEALGTLRPRINIVMLIPSAENMPAANAFKPGDIVTTLSGRTIEILNTD 352 DMGGAAAVLG ME +G LRP N++ +IPS +NM + A KP D++ +LSG+TIEILNTD Sbjct: 284 DMGGAAAVLGAMETIGELRPEQNVLCVIPSTDNMISGGAMKPDDVIVSLSGKTIEILNTD 343 Query: 353 AEGRVVLADALTYSLEWGAERIIDVATLTGAVLSVLGDIATGAVTNNEVMMEALQTASIR 412 AEGR+VLAD +TY+ + GA ++DVATLTG V+ LG TGA+TN++ + + A+ Sbjct: 344 AEGRLVLADGITYAKQHGASVLVDVATLTGGVVVALGTETTGAMTNDQSFYQQVADAAQE 403 Query: 413 AGEKIWRLPVFPEFREML-SSEVADIRNAAGRYGGASTAGLFIGEFAEGRPWVHLDIAGT 471 GE IW+LP+ + ++ + SS++AD+ N+ GR G A AG F+GEFAE PWVHLDIAGT Sbjct: 404 CGEAIWQLPITEKDKKRVKSSQMADLSNSPGREGHAIMAGTFLGEFAESTPWVHLDIAGT 463 Query: 472 AFLSKERGVNPKGATGVMVRTL 493 A +K P GATGVM RTL Sbjct: 464 ATANKATCFGPAGATGVMARTL 485 >ref|NP_653388.1| Peptidase_M17, Cytosol aminopeptidase family, catalytic domain [Bacillus anthracis A2012] ref|NP_847341.1| cytosol aminopeptidase [Bacillus anthracis str. Ames] gb|AAP28827.1| cytosol aminopeptidase [Bacillus anthracis str. Ames] Length = 494 Score = 325 bits (832), Expect = 2e-87, Method: Compositional matrix adjust. Identities = 174/361 (48%), Positives = 235/361 (65%), Gaps = 10/361 (2%) Query: 136 LGAYRRTHYKREQPVYAGLE--SAVFHLDRQAEAAESLEWSQGIRQGQAFGEATNLARXX 193 LG Y YK ++ LE +A+ AE A+ +E + + G G ATN AR Sbjct: 131 LGTYELQTYKSDKKDRVELEKFTAI-----TAEDAQEIEAALTV--GYVHGRATNSARTL 183 Query: 194 XXXXXXXXXXSALAMAAIEVAERHGFPAEVLDEQEIAQKGMGGLLAVGKGSVHPPRMIVI 253 + LA A+E+AE++ +VL+++E+ + GMG LLAV +GSV PP+MI + Sbjct: 184 VNMPPNVLTATKLAEYAVELAEKYDMDYKVLEKEEMEELGMGALLAVNQGSVEPPKMIAL 243 Query: 254 RYQGTSQWENVTAIVGKGITFDSGGISLKRAPGMEDMISDMGGAAAVLGVMEALGTLRPR 313 Y+G +W +V VGKGIT+D+GG SLK GM M DMGGAAAVLG ME +G LRP Sbjct: 244 IYKGKEEWTDVIGFVGKGITYDTGGYSLKPREGMVGMKGDMGGAAAVLGAMEIIGELRPE 303 Query: 314 INIVMLIPSAENMPAANAFKPGDIVTTLSGRTIEILNTDAEGRVVLADALTYSLEWGAER 373 N++ +IPS +N+ + AFKP D++T++SG+TIE+LNTDAEGR+ LAD +TY+ + GA Sbjct: 304 QNVIAVIPSTDNVVSGTAFKPDDVITSMSGKTIEVLNTDAEGRLALADGITYAKKLGANY 363 Query: 374 IIDVATLTGAVLSVLGDIATGAVTNNEVMMEALQTASIRAGEKIWRLPVFPEFREML-SS 432 +IDVATLTG V+ LG+ TGA+TNNE + E + AS+ E IW+LP+F +E + +S Sbjct: 364 LIDVATLTGGVIVALGNHTTGAMTNNEELFEQVLEASMETDESIWQLPIFDRDKERVRNS 423 Query: 433 EVADIRNAAGRYGGASTAGLFIGEFAEGRPWVHLDIAGTAFLSKERGVNPKGATGVMVRT 492 + AD+ N+ GR G A AG FIGEFAE PWVHLDIAGT+ S + P GATG MVRT Sbjct: 424 KFADLNNSPGREGHAVMAGTFIGEFAEDTPWVHLDIAGTSESSGAHDLGPAGATGAMVRT 483 Query: 493 L 493 L Sbjct: 484 L 484 >ref|NP_981353.1| cytosol aminopeptidase [Bacillus cereus ATCC 10987] gb|AAS43961.1| cytosol aminopeptidase [Bacillus cereus ATCC 10987] Length = 494 Score = 323 bits (827), Expect = 7e-87, Method: Compositional matrix adjust. Identities = 172/361 (47%), Positives = 231/361 (63%), Gaps = 10/361 (2%) Query: 136 LGAYRRTHYKREQPVYAGLE--SAVFHLDRQAEAAESLEWSQGIRQGQAFGEATNLARXX 193 LG Y YK ++ LE +A+ D Q E + G G ATN AR Sbjct: 131 LGTYELQTYKSDKKDRVELEKFTAITAEDTQ-------EIEAALTVGYVHGRATNSARTL 183 Query: 194 XXXXXXXXXXSALAMAAIEVAERHGFPAEVLDEQEIAQKGMGGLLAVGKGSVHPPRMIVI 253 + LA A+E+AE++ +VL+++E+ + GMG LLAV +GSV PP+MI + Sbjct: 184 VNMPPNVLTATKLAEYAVELAEKYDMDYKVLEKEEMEELGMGALLAVNQGSVEPPKMIAL 243 Query: 254 RYQGTSQWENVTAIVGKGITFDSGGISLKRAPGMEDMISDMGGAAAVLGVMEALGTLRPR 313 Y+G +W +V VGKGIT+D+GG SLK GM M DMGGAAAVLG ME +G LRP Sbjct: 244 IYKGKEEWTDVIGFVGKGITYDTGGYSLKPREGMVGMKGDMGGAAAVLGAMEIIGELRPE 303 Query: 314 INIVMLIPSAENMPAANAFKPGDIVTTLSGRTIEILNTDAEGRVVLADALTYSLEWGAER 373 N++ +IPS +N+ + AFKP D++T++SG+TIE+LNTDAEGR+ LAD +TY+ + GA Sbjct: 304 QNVIAVIPSTDNVVSGTAFKPDDVITSMSGKTIEVLNTDAEGRLALADGITYAKKLGANY 363 Query: 374 IIDVATLTGAVLSVLGDIATGAVTNNEVMMEALQTASIRAGEKIWRLPVFPEFREML-SS 432 +IDVATLTG V+ LG+ TGA+TNNE + E + AS+ E IW+LP+F +E + +S Sbjct: 364 LIDVATLTGGVIVALGNHTTGAMTNNEELFEQVLEASMETDESIWQLPIFDRDKERVRNS 423 Query: 433 EVADIRNAAGRYGGASTAGLFIGEFAEGRPWVHLDIAGTAFLSKERGVNPKGATGVMVRT 492 + AD+ N+ GR G A AG F+GEFAE PWVHLDIAGT+ S + P GATG MVRT Sbjct: 424 KFADLNNSPGREGHAVMAGTFLGEFAEDTPWVHLDIAGTSESSGAHDLGPAGATGAMVRT 483 Query: 493 L 493 L Sbjct: 484 L 484 >ref|NP_834593.1| Cytosol aminopeptidase [Bacillus cereus ATCC 14579] gb|AAP11794.1| Cytosol aminopeptidase [Bacillus cereus ATCC 14579] Length = 494 Score = 322 bits (825), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 169/359 (47%), Positives = 232/359 (64%), Gaps = 6/359 (1%) Query: 136 LGAYRRTHYKREQPVYAGLESAVFHLDRQAEAAESLEWSQGIRQGQAFGEATNLARXXXX 195 LG Y YK ++ LE + +E A+ +E + + G G ATN AR Sbjct: 131 LGTYELQTYKSDKKDRVELEK---FMAITSEDAQEIEAALTV--GYVHGRATNSARTLVN 185 Query: 196 XXXXXXXXSALAMAAIEVAERHGFPAEVLDEQEIAQKGMGGLLAVGKGSVHPPRMIVIRY 255 + LA A+E+AE++ +VL+++E+ GMG LLAV +GSV PP+MI + Y Sbjct: 186 MPPNVLTATKLAEYAVELAEKYDMDYKVLEKEEMEDLGMGALLAVNQGSVEPPKMIALIY 245 Query: 256 QGTSQWENVTAIVGKGITFDSGGISLKRAPGMEDMISDMGGAAAVLGVMEALGTLRPRIN 315 +G +W++V VGKGIT+D+GG SLK GM M DMGGAAAVLG ME +G LRP N Sbjct: 246 KGKEEWKDVIGFVGKGITYDTGGYSLKPREGMVGMKGDMGGAAAVLGAMEIIGELRPEQN 305 Query: 316 IVMLIPSAENMPAANAFKPGDIVTTLSGRTIEILNTDAEGRVVLADALTYSLEWGAERII 375 ++ +IPS +N+ + AFKP D++T++SG+TIE+LNTDAEGR+ LAD +TY+ + GA ++ Sbjct: 306 VIAVIPSTDNVVSGTAFKPDDVITSMSGKTIEVLNTDAEGRLALADGITYAKKLGANYLV 365 Query: 376 DVATLTGAVLSVLGDIATGAVTNNEVMMEALQTASIRAGEKIWRLPVFPEFREML-SSEV 434 DVATLTG V+ LG+ TGA+TNNE + E + AS+ E IW+LP+F +E + +S+ Sbjct: 366 DVATLTGGVIVALGNYTTGAMTNNEELFEQVLEASMETDEPIWQLPIFDRDKERVRNSKF 425 Query: 435 ADIRNAAGRYGGASTAGLFIGEFAEGRPWVHLDIAGTAFLSKERGVNPKGATGVMVRTL 493 AD+ N+ GR G A AG F+GEFAE PWVHLDIAGT+ + P GATG MVRTL Sbjct: 426 ADLNNSPGREGHAVMAGTFLGEFAEDTPWVHLDIAGTSETKGAHDLGPAGATGAMVRTL 484 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 675,445,433 Number of extensions: 25356063 Number of successful extensions: 62627 Number of sequences better than 1.0e-05: 836 Number of HSP's gapped: 61372 Number of HSP's successfully gapped: 837 Length of query: 514 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 384 Effective length of database: 404,274,708 Effective search space: 155241487872 Effective search space used: 155241487872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)