BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0078.p (367 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||S70528 sensor protein pilS - Myxococcus xanthus >gi|950652|... 172 1e-41 ref|NP_242786.1| two-component sensor histidine kinase [Bacillus... 169 1e-40 ref|NP_754648.1| Sensor protein atoS [Escherichia coli CFT073] >... 167 3e-40 ref|NP_416723.1| AtoS [Escherichia coli K12] >gi|732226|sp|Q0606... 166 8e-40 gb|AAA22800.1| SpoIIJ protein (gtg start codon) 160 4e-38 >pir||S70528 sensor protein pilS - Myxococcus xanthus gb|AAB40880.1| sensor protein Length = 525 Score = 172 bits (435), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 106/343 (30%), Positives = 175/343 (51%), Gaps = 24/343 (6%) Query: 26 EDVLEHIDSGILLFDEEGVLTFVNKQMYGILGLNRHSLLGCTLIHLLSNIQMNRFKKRQV 85 + +L + SG+ D + +T+VN GIL ++ + G + LL + Sbjct: 204 QQILSSMPSGLATCDAQRRVTYVNPAGCGILQVDPTQVAGVEVEALLPGV---------- 253 Query: 86 LHGYREMMKKGKPSYEFVDEYG---RYWKVSLHCGEHMKGSYLFTFKEISDYKLIEQTAY 142 ++ P E V G R +S+ E G+ L F++++ + +E Sbjct: 254 -----SVLAPRSPRSELVVGKGAKRRILGLSVTPLEGEPGALLMVFQDLTQLRRMEDDLK 308 Query: 143 QNDSLAMLGKLSASIAHEIRNPLTAIRGFIQLLHPHLHQLGKEEYAKIILAEIDRANDII 202 + D LA LG LSA +AHE+RNPL ++RG QLL ++ I++ E DR ++ Sbjct: 309 RADRLASLGALSAQLAHELRNPLASMRGSAQLLAQDARDDVAQKLTNILMRESDRLARLV 368 Query: 203 HEFLTSSKPSVPQVAMIPVSALLKEVVLLTESEALMKGCEINFYPLQEEMIISGDIKQMK 262 EFL ++P VP + +P+++L+ E + + + L + + E + +S D Q++ Sbjct: 369 EEFLRFARPPVPSLRRVPLASLVTETMDMLRVDPLARDVRVEVTA-PEPLPVSVDPDQLR 427 Query: 263 QVVLNMIRNAMEAITDKIDDSMGRIELGASREGAEVRIFISDNGKGMDICTLDRLFSPFF 322 QV++N++RN A + G +++ +R E RI I D+G + + LF PFF Sbjct: 428 QVLINLVRNGFLAAGPR-----GEVKVALTRSENEARIRIWDSGGSITEEMMGHLFEPFF 482 Query: 323 TTKENGTGLGLSVSDRIIKNHGGCISVSSRVNEGTRFVISLPL 365 TT++ GTGLGLS + II+ HGG I V S +EGT FV+ LPL Sbjct: 483 TTRDGGTGLGLSTAHSIIRAHGGSIRVRSNRDEGTEFVVGLPL 525 >ref|NP_242786.1| two-component sensor histidine kinase [Bacillus halodurans] pir||H83889 two-component sensor histidine kinase BH1920 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05639.1| two-component sensor histidine kinase [Bacillus halodurans] Length = 548 Score = 169 bits (428), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 113/339 (33%), Positives = 187/339 (55%), Gaps = 17/339 (5%) Query: 34 SGILLFDEEGVLTFVNKQMYGILGLNRHSLLGCTLIHLL---SNIQMNRFKKRQVLHGYR 90 +GI++ D+EG +T N + L+R +LG + + + + KK+++ + Sbjct: 210 NGIVITDKEGKITECNHSAEELFALDREDMLGKKIREVQPFGEYVDLAVRKKQRIDNMDL 269 Query: 91 EMMK-KGKPSYEFVDEYGRYWKVSLHCGEHMKGSYLFTFKEISDYKLIEQTAYQNDSLAM 149 + + K Y +D Y K + G+Y F++I+D +EQ QN+ + Sbjct: 270 NFINIENKMKYITMDVSPIYGK-----NQEFIGAY-GQFRDITDRVELEQQVIQNEKFSA 323 Query: 150 LGKLSASIAHEIRNPLTAIRGFIQLL--HPHLHQLGKEEYAKIILAEIDRANDIIHEFLT 207 +G+LSA +AHEIRNPLT I GF+ LL HL + K+ Y +I+ E++R ++++F+ Sbjct: 324 IGRLSAGLAHEIRNPLTPIIGFVNLLKNESHLDEKAKQ-YLRIMSDELERVRKLVNDFVI 382 Query: 208 SSKPSVPQVAMIPVSALLKEVVLLTESEALMKGCEINF-YPLQEEMIISGDIKQMKQVVL 266 SKP P++ + L+++V+ ES+ + ++ + + E + D QMKQV++ Sbjct: 383 VSKPEPPELKKTSLHTLVEQVIRFMESQGSLHNVQMEVNHSIDETDELLVDASQMKQVLI 442 Query: 267 NMIRNAMEAITDKIDDSMGRIELGASREGAEVRIFISDNGKGMDICTLDRLFSPFFTTKE 326 N++ NAM+A+ R+E G I + D G GM L++LF+PFFTTK+ Sbjct: 443 NLLTNAMDAMER---GGTIRVETGKDLGNGMFSIKVQDEGCGMSEDQLNQLFNPFFTTKD 499 Query: 327 NGTGLGLSVSDRIIKNHGGCISVSSRVNEGTRFVISLPL 365 +G GLGLS+ RIIKNHGG I+V S+ EG+ F I LP+ Sbjct: 500 DGLGLGLSICYRIIKNHGGTINVVSQQGEGSTFEILLPV 538 >ref|NP_754648.1| Sensor protein atoS [Escherichia coli CFT073] gb|AAN81216.1| Sensor protein atoS [Escherichia coli CFT073] Length = 612 Score = 167 bits (424), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 108/340 (31%), Positives = 178/340 (52%), Gaps = 8/340 (2%) Query: 28 VLEHIDSGILLFDEEGVLTFVNKQMYGILGLNRHSLLGCTLIHLLSNIQMNRFKKRQVLH 87 ++E+ G++ D +G +T +N I G RH L+G L N Q + H Sbjct: 271 IIENAADGVIAIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEH 330 Query: 88 GYREM-MKKGKPSYEFVDEYGRYWKVSLHCGEHMKGSYLFTFKEISDYKLIEQTAYQNDS 146 G + ++ P + E + M G+ L F +++ K ++ Q + Sbjct: 331 GTEHVALEISFPGRDRTIELSVTTSRIHNTHGEMIGA-LVIFSDLTARKETQRRMAQAER 389 Query: 147 LAMLGKLSASIAHEIRNPLTAIRGFIQLLHPHLHQLGKEEYAKIILAEIDRANDIIHEFL 206 LA LG+L A +AHE+RNPLTAIRG++Q+L +EY ++L EID N +I + L Sbjct: 390 LATLGELMAGVAHEVRNPLTAIRGYVQILRQQTRDPIHQEYLSVVLKEIDSINKVIQQLL 449 Query: 207 TSSKPSVPQVAMIPVSALLKEVVLLTESEALMKGCEINFYPLQEEMIISGDIKQMKQVVL 266 S+P Q + ++AL++E ++L ++ + + E I+ D + +KQV+L Sbjct: 450 EFSRPRHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLL 509 Query: 267 NMIRNAMEAITDKIDDSMGRIELGASR-EGAEVRIFISDNGKGMDICTLDRLFSPFFTTK 325 N++ NA++AI+ + G+I + + ++ I I DNG G+D+ ++F PFFTTK Sbjct: 510 NILINAVQAISAR-----GKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTK 564 Query: 326 ENGTGLGLSVSDRIIKNHGGCISVSSRVNEGTRFVISLPL 365 +GTGLGL++S RII H G I V+S G F + LP+ Sbjct: 565 ASGTGLGLALSQRIINAHQGDIRVASLPGYGATFTLILPI 604 >ref|NP_416723.1| AtoS [Escherichia coli K12] sp|Q06067|ATOS_ECOLI Sensor protein atoS pir||A64992 sensor protein AtoS (EC 2.7.3.-) - Escherichia coli (strain K-12) gb|AAA23449.1| ORF-1 dbj|BAA16015.1| Sensor protein AtoS (EC 2.7.3.-). [Escherichia coli] gb|AAC75279.1| sensor protein AtoS for response regulator AtoC [Escherichia coli K12] Length = 608 Score = 166 bits (420), Expect = 8e-40, Method: Compositional matrix adjust. Identities = 108/340 (31%), Positives = 178/340 (52%), Gaps = 8/340 (2%) Query: 28 VLEHIDSGILLFDEEGVLTFVNKQMYGILGLNRHSLLGCTLIHLLSNIQMNRFKKRQVLH 87 ++E+ G++ D +G +T +N I G RH L+G L N Q + H Sbjct: 267 IIENAADGVIAIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEH 326 Query: 88 GYREM-MKKGKPSYEFVDEYGRYWKVSLHCGEHMKGSYLFTFKEISDYKLIEQTAYQNDS 146 G + ++ P + E + M G+ L F +++ K ++ Q + Sbjct: 327 GTEHVALEISFPGRDRTIELSVTTSRIHNTHGEMIGA-LVIFSDLTARKETQRRMAQAER 385 Query: 147 LAMLGKLSASIAHEIRNPLTAIRGFIQLLHPHLHQLGKEEYAKIILAEIDRANDIIHEFL 206 LA LG+L A +AHE+RNPLTAIRG++Q+L +EY ++L EID N +I + L Sbjct: 386 LATLGELMAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLL 445 Query: 207 TSSKPSVPQVAMIPVSALLKEVVLLTESEALMKGCEINFYPLQEEMIISGDIKQMKQVVL 266 S+P Q + ++AL++E ++L ++ + + E I+ D + +KQV+L Sbjct: 446 EFSRPRHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLL 505 Query: 267 NMIRNAMEAITDKIDDSMGRIELGASR-EGAEVRIFISDNGKGMDICTLDRLFSPFFTTK 325 N++ NA++AI+ + G+I + + ++ I I DNG G+D+ ++F PFFTTK Sbjct: 506 NILINAVQAISAR-----GKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTK 560 Query: 326 ENGTGLGLSVSDRIIKNHGGCISVSSRVNEGTRFVISLPL 365 +GTGLGL++S RII H G I V+S G F + LP+ Sbjct: 561 ASGTGLGLALSQRIINAHQGDIRVASLPGYGATFTLILPI 600 >gb|AAA22800.1| SpoIIJ protein (gtg start codon) Length = 606 Score = 160 bits (405), Expect = 4e-38, Method: Compositional matrix adjust. Identities = 92/238 (38%), Positives = 143/238 (60%), Gaps = 8/238 (3%) Query: 128 FKEISDYKLIEQTAYQNDSLAMLGKLSASIAHEIRNPLTAIRGFIQLLHPHLHQLGKEEY 187 ++IS+ K E+ +++ L++ G+L+A IAHEIRNPLTAI+GF+QL+ P + G E Y Sbjct: 374 LRDISERKQTEELMLKSEKLSIAGQLAAGIAHEIRNPLTAIKGFLQLMKPTME--GNEHY 431 Query: 188 AKIILAEIDRANDIIHEFLTSSKPSVPQVA-MIPVSALLKEVVLLTESEALMKGCEINFY 246 I+ +E+ R I+ E L +KP V + + L+ EV L E++A + G I Sbjct: 432 FDIVFSELSRIELILSELLMLAKPQQNAVKEYLNLKKLIGEVSALLETQANLNGIFIRTS 491 Query: 247 PLQEEMIISGDIKQMKQVVLNMIRNAMEAITDKIDDSMGRIELGASREGAEVRIFISDNG 306 ++ + I+GD Q+KQV +N+I+NA+E++ D G +++ + + V + + D G Sbjct: 492 YEKDSIYINGDQNQLKQVFINLIKNAVESMPDG-----GTVDIIITEDEHSVHVTVKDEG 546 Query: 307 KGMDICTLDRLFSPFFTTKENGTGLGLSVSDRIIKNHGGCISVSSRVNEGTRFVISLP 364 +G+ L+R+ PF TTKE GTGLGL V+ II+NH G I V S +GT F IS P Sbjct: 547 EGIPEKVLNRIGEPFLTTKEKGTGLGLMVTFNIIENHQGVIHVDSHPEKGTAFKISFP 604 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 517,709,656 Number of extensions: 21376678 Number of successful extensions: 73660 Number of sequences better than 1.0e-05: 4536 Number of HSP's gapped: 67865 Number of HSP's successfully gapped: 4554 Length of query: 367 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 240 Effective length of database: 412,420,005 Effective search space: 98980801200 Effective search space used: 98980801200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)