BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0035.p (334 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00181100.1| hypothetical protein Mther083901 [Moorella th... 103 7e-21 ref|NP_691616.1| spore germination protein [Oceanobacillus iheye... 93 1e-17 ref|NP_780992.1| spore germination protein A2 [Clostridium tetan... 88 3e-16 ref|NP_624074.1| spore germination protein homolog yndE [Thermoa... 78 3e-13 ref|NP_977104.1| spore germination protein GerLB [Bacillus cereu... 78 3e-13 >ref|ZP_00181100.1| hypothetical protein Mther083901 [Moorella thermoacetica ATCC 39073] Length = 342 Score = 103 bits (256), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 84/317 (26%), Positives = 138/317 (43%) Query: 6 GSSAPLVAFTGVLISFFSFWLLAALCRRFPRESLFVFSRRLIGRPLAMFFTLIIWLIFIM 65 G LV+ + S + +LL AL +FP L FSRRL+G+ LA LI+ + ++ Sbjct: 18 GVDTWLVSPLETIFSLGAIYLLTALVMKFPDLDLVGFSRRLVGKWLAWLLGLIVLVYWVG 77 Query: 66 LTGLTARQFGDVATSVLFNKTPIEATVVLMLLICQLSARRNIIKFSYIHFFYXXXXXXXX 125 LT R D S L + TP + LL+ A + + + Sbjct: 78 LTAEVGRVTADTIKSSLLSHTPDAVVLSSYLLVAAYLAGKGLEPLARASIIIVIFTLPIT 137 Query: 126 XXXXXXSMRDVDLLNLQPVLTAPSASFWKGALEASYLFQSSFIITLLVPFMQLPKQAVRX 185 + + L N P+L K AL + + +LVPF++ P++A R Sbjct: 138 LLLFALVIPRIHLDNFLPILPHGPWPVIKLALWRISNAEEMSLFLILVPFLKEPRRAWRA 197 Query: 186 XXXXXXXXXXVYLLIVIASVGMFGVEETKLLIYPTLETARSAVVGDGFLERMDALFIVIW 245 V + I+ G+ GV++ K + P L + A F+ER+ +FI +W Sbjct: 198 ASYGFLTVMAVVITIITTCQGVLGVDQLKYTLIPGLTVTQLAEFAGAFIERISLIFISVW 257 Query: 246 VISVXXXXXXXXXXXXXLLQNLLAFRDQRMTSSVLLPLVFAIAMLPKNVFETYTWTLLLG 305 +I V LL LL +D +M + LP+V+ +A P N FE ++ L Sbjct: 258 IILVFPTASALLWASSYLLGRLLNLKDYKMLAFYQLPVVYYLAWRPGNPFEVKSFFFFLQ 317 Query: 306 KMSMILMVGYPALLWAV 322 + ++++VG P+LL+ V Sbjct: 318 PLGLVVLVGIPSLLYLV 334 >ref|NP_691616.1| spore germination protein [Oceanobacillus iheyensis HTE831] dbj|BAC12651.1| spore germination protein [Oceanobacillus iheyensis HTE831] Length = 370 Score = 92.8 bits (229), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 68/319 (21%), Positives = 148/319 (46%), Gaps = 5/319 (1%) Query: 15 TGVLISFFSFWLLAALCRRFPRESLFVFSRRLIGRPLAMFFTLIIWLIFIMLTGLTARQF 74 T ++S W++A + +FP +S ++ L+ RP+A+ +++ ++ + G Sbjct: 51 TAGILSIMITWVIANIASKFPNQSFLDYASFLLSRPVAIAISVLYIIVCWFIVGYELAFI 110 Query: 75 GDVATSVLFNKTPIEATVV--LMLLICQLSARRNIIKFSYIHFFYXXXXXXXXXXXXXXS 132 D++ LF++TP E V+ L++++ +S RN + F + S Sbjct: 111 ADISQHYLFDRTPTEVVVLAFLLVIVYAVSGSRN--AMFRMCFGFMAFSIIATIVLVFMS 168 Query: 133 MRDVDLLNLQPVLTAPSASFWKGALEASYLFQSSFIITLLVPFMQLPKQAVRXXXXXXXX 192 + D +L PV T ++ K + FI+ + + PK+ + Sbjct: 169 LPFGDFDHLLPVFTTDWKTYIKELPNGILSYTGGFILLFYMKLNRDPKKGPKSAAIGVSV 228 Query: 193 XXXVYLLIVIASVGMFGVEETKLLIYPTLETARSAVVGDGFLERMDALFIVIWVISVXXX 252 +Y+ I +A + + G T IYP +E A + + GFL+R+++L+ VIW+ ++ Sbjct: 229 VVILYVSIYLACIAVLGNVTTANTIYPLVELANT-IHSAGFLDRIESLYYVIWITTIFLT 287 Query: 253 XXXXXXXXXXLLQNLLAFRDQRMTSSVLLPLVFAIAMLPKNVFETYTWTLLLGKMSMILM 312 ++ +L +++ + + P++F++++LP N ++ + ++G S+IL Sbjct: 288 CILAYDVSVMIITDLFPKANKKYITFGICPIIFSLSILPANYYQLGLYASIVGYSSIILT 347 Query: 313 VGYPALLWAVYGVRRFRKK 331 V L +Y +R +K+ Sbjct: 348 VLTIMTLNMMYFFKRKKKQ 366 >ref|NP_780992.1| spore germination protein A2 [Clostridium tetani E88] gb|AAO34929.1| spore germination protein A2 [Clostridium tetani E88] Length = 369 Score = 87.8 bits (216), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 63/282 (22%), Positives = 127/282 (45%), Gaps = 6/282 (2%) Query: 16 GVLISFFSFWLLAALCRRFPRESLFVFSRRLIGRPLAMFFTLIIWLIFIMLTGLTARQFG 75 G +I++ + +L+ ++ + + + G+ L L I ++ + R F Sbjct: 50 GGIITYVAAYLIYKCIQKSDYKDCYDIFQNSFGKVLGNIVALSIVAYGLIFISVALRAFT 109 Query: 76 DVATSVLFNKTPIEATVVLMLLICQLSAR---RNIIKFSYIHFFYXXXXXXXXXXXXXXS 132 + L TP E V+M+L R ++I+F+ I F+ S Sbjct: 110 EEIKLYLLKDTPTEFIFVVMILSGVYLVRAELEHLIRFNEISFW---IMFIPIVFVLLVS 166 Query: 133 MRDVDLLNLQPVLTAPSASFWKGALEASYLFQSSFIITLLVPFMQLPKQAVRXXXXXXXX 192 VD NL PV S++KG++ Y F I+ L+VP ++ + + Sbjct: 167 AYGVDFTNLLPVFKNKPLSYFKGSINVFYRFGGIGILFLIVPILKKKDKTPKVMKKSLLF 226 Query: 193 XXXVYLLIVIASVGMFGVEETKLLIYPTLETARSAVVGDGFLERMDALFIVIWVISVXXX 252 Y++I I S+ FG E++K+L++PT+ S + F+++ + + + IW++ Sbjct: 227 ISVFYIVIFILSIATFGEEQSKILLWPTITMISSIDIPGSFIQKWEGIVMAIWIMFYYTS 286 Query: 253 XXXXXXXXXXLLQNLLAFRDQRMTSSVLLPLVFAIAMLPKNV 294 +L+N+L D +++S++L+P ++ I + P+NV Sbjct: 287 FINYYYLSSDVLKNILKLDDIKLSSAILVPFIYIIGLYPENV 328 >ref|NP_624074.1| spore germination protein homolog yndE [Thermoanaerobacter tengcongensis] gb|AAM25678.1| spore germination protein homolog yndE [Thermoanaerobacter tengcongensis] Length = 365 Score = 78.2 bits (191), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 57/294 (19%), Positives = 124/294 (42%) Query: 30 LCRRFPRESLFVFSRRLIGRPLAMFFTLIIWLIFIMLTGLTARQFGDVATSVLFNKTPIE 89 + +FP E+ ++ +L+ +P+A+ ++++ + L R FG+V L TPIE Sbjct: 62 ISSKFPTETFSEYASKLVTKPVAVVLSIVLIFYLSVFVSLDVRIFGEVLKIYLLPSTPIE 121 Query: 90 ATVVLMLLICQLSARRNIIKFSYIHFFYXXXXXXXXXXXXXXSMRDVDLLNLQPVLTAPS 149 ++ ML R + + + ++ DVDL N P + Sbjct: 122 TIIITMLFASAYLVRYGLEPIARMSEILFPIMVIPLLLLFLPALSDVDLTNFLPFMRVSL 181 Query: 150 ASFWKGALEASYLFQSSFIITLLVPFMQLPKQAVRXXXXXXXXXXXVYLLIVIASVGMFG 209 F KG +Y F ++ ++ P++ ++ + Y+ I +G+FG Sbjct: 182 MKFLKGVFVTTYSFVGFELLYMVFPYVIDQEKLKKSVNASIISTTLFYMYICFFVIGIFG 241 Query: 210 VEETKLLIYPTLETARSAVVGDGFLERMDALFIVIWVISVXXXXXXXXXXXXXLLQNLLA 269 +ETK ++P L +S F+E ++ + + IW ++ +L L+ Sbjct: 242 YKETKEQLWPFLTLIKSINFPVFFIENVEGIVMAIWTFTIFTSIYSFHYFAVLVLSKLIK 301 Query: 270 FRDQRMTSSVLLPLVFAIAMLPKNVFETYTWTLLLGKMSMILMVGYPALLWAVY 323 R+ L+P ++ +A++P ++ Y + L+ + I + + ++ A + Sbjct: 302 AREHSYLVLPLIPPMYLMALIPDSIVTLYKYANLVSQYLSIFFISFLPIILAFF 355 >ref|NP_977104.1| spore germination protein GerLB [Bacillus cereus ATCC 10987] gb|AAS39712.1| spore germination protein GerLB [Bacillus cereus ATCC 10987] Length = 374 Score = 77.8 bits (190), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 64/308 (20%), Positives = 133/308 (43%), Gaps = 8/308 (2%) Query: 25 WLLAALCRRFPRESLFVFSRRLIGRPLAMFFTLIIWLIFIMLTGLTARQFGDVATSVLFN 84 W + + FP+++ ++ + +P+A ++++ L F LT +R ++ + LF+ Sbjct: 61 WFVTRVAILFPKQNFVQYTSAHLTKPVAYTISMVLVLTFAALTAYESRMIAIISQTYLFS 120 Query: 85 KTPIE--ATVVLMLLICQLSARRNIIKFSYIHFFYXXXXXXXXXXXXXXSMRDVDLLNLQ 142 +TP++ + L+++I ++ R + + F ++ +++ NL Sbjct: 121 ETPVQLLSFFFLLVVIYGIAGSRAALLRLNVMFLPIVLIAIVLLSLLNINLMEIN--NLF 178 Query: 143 PVLTAPSASFWKGALEASYLFQSSFIITLLVPFM---QLPKQAVRXXXXXXXXXXXVYLL 199 P + + G + + F F + L + + K+A Y+L Sbjct: 179 PAFQTEVSQYAVGVKNSIFTFIG-FEVALFYAVLLNDKTAKKAPMAVAKAVIVNVLSYIL 237 Query: 200 IVIASVGMFGVEETKLLIYPTLETARSAVVGDGFLERMDALFIVIWVISVXXXXXXXXXX 259 I + + +F T+ L YPT+E + +G GFLER DA+F W+I++ Sbjct: 238 IYVTCISVFTYMTTRGLTYPTIELGKEIEIGGGFLERFDAIFFTTWIITIYNTTAMYYDV 297 Query: 260 XXXLLQNLLAFRDQRMTSSVLLPLVFAIAMLPKNVFETYTWTLLLGKMSMILMVGYPALL 319 L + + + PL+F + MLP N+ ++ L + M +V P L+ Sbjct: 298 ASLLFCAMFPKVKKHIFIFGSAPLIFMLNMLPGNLNTLSSYGTYLAWIDMGFVVLAPLLV 357 Query: 320 WAVYGVRR 327 + VY ++R Sbjct: 358 FIVYKIKR 365 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.333 0.144 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 384,299,757 Number of extensions: 12463193 Number of successful extensions: 44488 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 44465 Number of HSP's successfully gapped: 22 Length of query: 334 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 208 Effective length of database: 415,135,104 Effective search space: 86348101632 Effective search space used: 86348101632 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.5 bits) S2: 126 (53.1 bits)