BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0018.p (95 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_845612.1| RNA polymerase sigma-70 factor, ECF subfamily [... 96 2e-19 ref|NP_657186.1| Sigma70_ECF, Sigma-70 factor (ECF subfamily) [B... 96 2e-19 ref|NP_388833.1| similar to RNA polymerase ECF-type sigma factor... 92 3e-18 gb|AAP68417.1| SigM [Bacillus subtilis subsp. spizizenii] 92 4e-18 ref|NP_655022.1| Sigma70_ECF, Sigma-70 factor (ECF subfamily) [B... 86 2e-16 >ref|NP_845612.1| RNA polymerase sigma-70 factor, ECF subfamily [Bacillus anthracis str. Ames] gb|AAP27098.1| RNA polymerase sigma-70 factor, ECF subfamily [Bacillus anthracis str. Ames] Length = 166 Score = 96.3 bits (238), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 44/90 (48%), Positives = 62/90 (68%), Gaps = 3/90 (3%) Query: 6 LDELYQIYVQDIYAYLRSLCHDHYLAEDLMQETFYRAYLYLENCREDKIKPWLFRVAYNA 65 ++E+Y+IY+ D+Y YL SL H+ AEDLMQETFYRAYLYLE+ K+K WLF+VAY+ Sbjct: 3 IEEIYKIYINDVYRYLFSLSRSHHAAEDLMQETFYRAYLYLEDYENQKVKSWLFKVAYHT 62 Query: 66 FVDHTRKAKRRITAAYTCFSPLQIVQLSEN 95 F+D RK K+ ++ L+ +Q E+ Sbjct: 63 FIDFVRKEKK---VSFVGTEELEAIQAGES 89 >ref|NP_657186.1| Sigma70_ECF, Sigma-70 factor (ECF subfamily) [Bacillus anthracis A2012] Length = 166 Score = 96.3 bits (238), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 44/90 (48%), Positives = 62/90 (68%), Gaps = 3/90 (3%) Query: 6 LDELYQIYVQDIYAYLRSLCHDHYLAEDLMQETFYRAYLYLENCREDKIKPWLFRVAYNA 65 ++E+Y+IY+ D+Y YL SL H+ AEDLMQETFYRAYLYLE+ K+K WLF+VAY+ Sbjct: 3 IEEIYKIYINDVYRYLFSLSRSHHAAEDLMQETFYRAYLYLEDYENQKVKSWLFKVAYHT 62 Query: 66 FVDHTRKAKRRITAAYTCFSPLQIVQLSEN 95 F+D RK K+ ++ L+ +Q E+ Sbjct: 63 FIDFVRKEKK---VSFVGTEELEAIQAGES 89 >ref|NP_388833.1| similar to RNA polymerase ECF-type sigma factor [Bacillus subtilis] pir||C69826 RNA polymerase ECF-type sigma factor homolog yhdM - Bacillus subtilis emb|CAA74497.1| hypothetical protein [Bacillus subtilis] emb|CAB12791.1| RNA polymerase ECF(extracytoplasmic function)-type sigma factor (sigma-M) [Bacillus subtilis subsp. subtilis str. 168] Length = 163 Score = 92.0 bits (227), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 39/83 (46%), Positives = 60/83 (72%) Query: 5 SLDELYQIYVQDIYAYLRSLCHDHYLAEDLMQETFYRAYLYLENCREDKIKPWLFRVAYN 64 ++DE+YQ+Y+ D+Y +L S+ D +LAEDL+QETF RAY+++ + K+KPWLF+VA N Sbjct: 2 TIDEIYQMYMNDVYRFLLSMTKDKHLAEDLLQETFMRAYIHIHSYDHSKVKPWLFQVARN 61 Query: 65 AFVDHTRKAKRRITAAYTCFSPL 87 AF+D+ RK K+ +T + L Sbjct: 62 AFIDYVRKHKKEVTISDDLIGSL 84 >gb|AAP68417.1| SigM [Bacillus subtilis subsp. spizizenii] Length = 163 Score = 92.0 bits (227), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 39/83 (46%), Positives = 60/83 (72%) Query: 5 SLDELYQIYVQDIYAYLRSLCHDHYLAEDLMQETFYRAYLYLENCREDKIKPWLFRVAYN 64 ++DE+YQ+Y+ D+Y +L S+ D +LAEDL+QETF RAY+++ + K+KPWLF+VA N Sbjct: 2 TIDEIYQMYMNDVYRFLLSMTKDKHLAEDLLQETFMRAYIHIHSYDHSKVKPWLFQVARN 61 Query: 65 AFVDHTRKAKRRITAAYTCFSPL 87 AF+D+ RK K+ +T + L Sbjct: 62 AFIDYVRKHKKEVTISDDLIGSL 84 >ref|NP_655022.1| Sigma70_ECF, Sigma-70 factor (ECF subfamily) [Bacillus anthracis A2012] ref|NP_843600.1| RNA polymerase sigma-70 factor, ECF subfamily [Bacillus anthracis str. Ames] gb|AAP25086.1| RNA polymerase sigma-70 factor, ECF subfamily [Bacillus anthracis str. Ames] Length = 161 Score = 86.3 bits (212), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 40/83 (48%), Positives = 58/83 (69%) Query: 2 KPNSLDELYQIYVQDIYAYLRSLCHDHYLAEDLMQETFYRAYLYLENCREDKIKPWLFRV 61 + SL+E+Y ++QD++ YL SL D + AEDLMQETFYR ++++ + ++I+PWLF + Sbjct: 3 RKQSLEEIYSEHMQDLFRYLLSLTGDSHFAEDLMQETFYRMLVHIDYYKGEEIRPWLFTI 62 Query: 62 AYNAFVDHTRKAKRRITAAYTCF 84 AYNAF+D RK KR T T F Sbjct: 63 AYNAFIDWYRKEKRYKTTTITEF 85 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.138 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 132,763,017 Number of extensions: 4374518 Number of successful extensions: 11975 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 12127 Number of HSP's successfully gapped: 15 Length of query: 95 Length of database: 757,237,578 Length adjustment: 63 Effective length of query: 32 Effective length of database: 586,186,341 Effective search space: 18757962912 Effective search space used: 18757962912 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 120 (50.8 bits)