BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0011.p (1735 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_623945.1| S-layer homology domain [Thermoanaerobacter ten... 145 8e-33 ref|NP_624131.1| Subtilisin-like serine proteases [Thermoanaerob... 131 2e-28 ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella th... 119 9e-25 gb|AAO88279.1| endo-beta-N-acetylglucosaminidase [Bacillus circu... 112 9e-23 sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pu... 107 2e-21 >ref|NP_623945.1| S-layer homology domain [Thermoanaerobacter tengcongensis] gb|AAM25549.1| S-layer homology domain [Thermoanaerobacter tengcongensis] Length = 1321 Score = 145 bits (366), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 117/393 (29%), Positives = 180/393 (45%), Gaps = 37/393 (9%) Query: 1353 KQIEASKKALAAVSE-QLTAKLGAGLASELKVSLTVEVPAMGDGVNKLNTSLPSEILKVL 1411 K IE++ KA+ V E +++ + E++ LT V L +P +L ++ Sbjct: 949 KVIESADKAVLVVDEIKVSKDIKDSTRKEVQFDLT---NISTKSVKAL--EIPVSVLNLI 1003 Query: 1412 QDN------KVDGLKLQMGTTGFTIEPDTFGTVEAGQKITLAAEVVENAVIGKPGQAEPL 1465 +N K D + L+ + + + + ITL E K + E Sbjct: 1004 AENQKDVIVKSDEIALKFESKVLAVSKEVLDLINKEGSITLTIE--------KKSKPEAN 1055 Query: 1466 ATLPV---MEFRATVGDKAVKHFEKPVNVTFDVSAINTSKYPAENLERLTVYVLNETSLT 1522 A PV + G+ +K E PV +TF+V A+++ ++ VY LNE + Sbjct: 1056 AYKPVSNAYDITIKAGESKIK-LESPVKLTFEVK-------DAKDIRKVGVYYLNEATGK 1107 Query: 1523 WEAVGGKYDPVTQTVSALRGHFSRYTVMIGSASFKDVADNHWAKKEINYLLTKGILEQTA 1582 WE VGGK D T TV+A HFS Y FKDV + WA IN L +K I++ Sbjct: 1108 WEYVGGKVDKNTNTVTAEVKHFSTYGAFEYDKQFKDVPKDFWAYDVINVLASKHIIKGMD 1167 Query: 1583 E--FNPSGKVTRAQFANWISRAYGLDGSGLTLPFSDVAAGSAGYNGVAAAYEAGIITGKS 1640 + F P+ K+TRA+FA + RA G+ F DV AG+ N + AAY AGI+ G Sbjct: 1168 DDNFAPNAKITRAEFAALMIRALGIPEEPYKGEFEDVKAGAWYANAIEAAYRAGIMMGDG 1227 Query: 1641 STVFDPEATISRQEIATMLARALTLYNGAKTVADPGAVNAAYTDGGKIAKWAAAGVALAN 1700 + P+ I+R+E+A ++ R G K + N + D I++WA VA A Sbjct: 1228 KKM-RPDDPITREEMAAVIMRVYAKMTGYK---EENIGNTVFKDNDSISEWARNVVANAA 1283 Query: 1701 RTDLFKGFEDGTFRPXXXXXXXXXXXLIYRLYQ 1733 + + KG+ED TFR L+YR+ + Sbjct: 1284 KLGIVKGYEDNTFRAKNNATRAEGVTLLYRILE 1316 >ref|NP_624131.1| Subtilisin-like serine proteases [Thermoanaerobacter tengcongensis] gb|AAM25735.1| Subtilisin-like serine proteases [Thermoanaerobacter tengcongensis] Length = 1999 Score = 131 bits (329), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 109/409 (26%), Positives = 187/409 (45%), Gaps = 44/409 (10%) Query: 1331 SKEHVTLEGNTVSSQLTEELVSKQI-EASKKALAAVSEQLTAKLGAGLASELKVSLTVEV 1389 S+ V +EGNT + E V+ +I +A+KK E+ LT Sbjct: 1624 SQGKVVVEGNTTVLVVEENKVANEIKDATKK-------------------EVVFDLT--- 1661 Query: 1390 PAMGDGVNKLNTSLPSEILKVLQDNKVDGLKLQMGTTGFTIEPDTFGTVEAGQKITLAAE 1449 +GD +K +P ++L ++ +N + ++ G +I+P E + Sbjct: 1662 -NVGDTSSKA-IEVPVKVLNMIAENN-KNVVVKSGEVALSIDPKAMTVSEETLNLINKTG 1718 Query: 1450 VVENAVIGKPGQAEPLATLPV---MEFRATVGDKAVKHFEKPVNVTFDVSAINTSKYPAE 1506 V+ V+ G+ + +PV + G+ +K + PV +TF+V + + Sbjct: 1719 SVK-LVVEDKGKKVANSLIPVSNAYDITIKTGENKIK-LDSPVKLTFEVKDV-------K 1769 Query: 1507 NLERLTVYVLNETSLTWEAVGGKYDPVTQTVSALRGHFSRYTVMIGSASFKDVADNHWAK 1566 ++ ++ +Y LNE + WE VGGK D T++ HFS Y FKDV + W Sbjct: 1770 DIRKVGIYYLNEVTGKWEYVGGKIDKNANTITFEAKHFSTYGAFEYDKQFKDVEKDFWGY 1829 Query: 1567 KEINYLLTKGILEQTAE--FNPSGKVTRAQFANWISRAYGLDGSGLTLPFSDVAAGSAGY 1624 IN L ++ I++ E F P+ K+TRA+FA + RA G+ F DV G Sbjct: 1830 DVINVLASRHIIKGMTEDTFAPNAKITRAEFAALMIRALGIPEEPYKGEFEDVKEGMWYA 1889 Query: 1625 NGVAAAYEAGIITGKSSTVFDPEATISRQEIATMLARALTLYNGAKTVADPGAVNAAYTD 1684 N + AAY+ GI+ G + P+ I+R+E+A ++ R + G K + N + D Sbjct: 1890 NAIEAAYQEGIMLGDGKKM-RPDDPITREEMAAVIMRVYSKLAGYK---EENIGNTTFAD 1945 Query: 1685 GGKIAKWAAAGVALANRTDLFKGFEDGTFRPXXXXXXXXXXXLIYRLYQ 1733 KI++WA VA A + + +G++D TF+P ++YR+ + Sbjct: 1946 NNKISEWARNVVANAVKLGIVRGYDDNTFKPKDNATRAEAAAMLYRILE 1994 >ref|ZP_00181177.1| hypothetical protein Mther075501 [Moorella thermoacetica ATCC 39073] Length = 368 Score = 119 bits (297), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 88/255 (34%), Positives = 124/255 (48%), Gaps = 20/255 (7%) Query: 1488 PVNVTFDVSAINTSKYPAENL---ERLTVYVLNETSLTWEAVGGKYDPVTQTVSALRGHF 1544 P TF V K+ +L E L + ++T ++W A+ D + A HF Sbjct: 119 PDGTTFAVPVTLYLKFALPSLVKPENLALAWYDKTKVSWVAIPAVVDVSKGLILARINHF 178 Query: 1545 SRYTVMIGSA--SFKDV--ADNHWAKKEINYLLTKGILEQT--AEFNPSGKVTRAQFANW 1598 S Y V + A +F DV A WA+ I L GI+ + F P VTRA+FA+ Sbjct: 179 SDYAVFVKEARKAFLDVTPASFGWAQDTIETLAGAGIVAGVDGSHFEPGRAVTRAEFASL 238 Query: 1599 ISRAYGLDG-SGLTLPFSDVAAGSAGYNG-VAAAYEAGIITGKSSTVFDPEATISRQEIA 1656 + +A GL+G +G PF DV G A Y G V AA G++ G F P+ I+R+E+A Sbjct: 239 LVKALGLEGKAGEKNPFKDVK-GDAWYAGAVTAAASNGLVKGYEDGTFRPDNVITREELA 297 Query: 1657 TMLARALTLYNGAKTVADPGAVNAAYTDGGKIAKWAAAGVALANRTDLFKGFEDGTFRPX 1716 ML RA+ L G + +A + D K++ WA VA+A L KGFEDGTFRP Sbjct: 298 AMLVRAVNLPAGEEKLA--------FKDNDKVSTWARKSVAVAAAHGLVKGFEDGTFRPG 349 Query: 1717 XXXXXXXXXXLIYRL 1731 ++YR+ Sbjct: 350 DAASRAECAVMVYRV 364 >gb|AAO88279.1| endo-beta-N-acetylglucosaminidase [Bacillus circulans] Length = 1936 Score = 112 bits (280), Expect = 9e-23, Method: Compositional matrix adjust. Identities = 79/252 (31%), Positives = 126/252 (50%), Gaps = 16/252 (6%) Query: 1482 VKHFEKPVNVTFDVSAINTSKYPAENLERLTVYVLNETSLTWEAVGGKYDPVTQTVSALR 1541 + F++P+ ++ + + N E++ +Y ++ + +GG+Y ++A Sbjct: 1696 LSEFDQPITISLKIES-------GVNPEQVGIYYISGNGKL-DYIGGEYR--DGELAAEV 1745 Query: 1542 GHFSRYTVMIGSASFKDVADNHWAKKEINYLLTKGILEQTAE--FNPSGKVTRAQFANWI 1599 HFS+Y V+ F DV HWA+ I+ L ++ +++ T+E F P VTRA+F + Sbjct: 1746 THFSQYAVLKVVKVFDDVPAGHWAEGVISKLTSRLMVDGTSETTFEPERVVTRAEFTALL 1805 Query: 1600 SRAYGLDGSGLTLPFSDVAAGSAGYNGVAAAYEAGIITGKSSTVFDPEATISRQEIATML 1659 +RA L G T F+DV AG + V AA EAGI GKS+ F+P+A I+R+E+ M Sbjct: 1806 ARALKLTAGG-TPTFADVKAGDWYADAVTAAVEAGIAEGKSAGQFEPQARITREEMVVMT 1864 Query: 1660 ARALTLYNGAKTVADPGAVNAAYTDGGKIAKWAAAGVALANRTDLFKGFEDGTFRPXXXX 1719 RA YN AK V A++TD +I+ WA V A L +G G F P Sbjct: 1865 MRA---YNKAKDKGPSTGVEASFTDENQISAWAVEQVKAAAALQLIQGRAQGKFEPQGTA 1921 Query: 1720 XXXXXXXLIYRL 1731 +I+ + Sbjct: 1922 TRAEAVQVIFNM 1933 >sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pullulanase) (Pullulanase type II) [Includes: Alpha-amylase (1,4-alpha-D-glucan glucanohydrolase); Pullulanase (1,4-alpha-D-glucan glucanohydrolase) (Alpha-dextrin endo-1,6-alpha-glucosidase)] Length = 1861 Score = 107 bits (268), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 78/249 (31%), Positives = 117/249 (46%), Gaps = 14/249 (5%) Query: 1487 KPVNVTFDVSAINTSKYPAENLERLTVYVLNETSLTWEAVGGKYDPVTQTVSALRGHFSR 1546 KPV VT ++S N + ++ VY N T+ WE VGGK D + T++ HFS+ Sbjct: 1620 KPVEVTLNISKANDPR-------KVAVYYYNPTTNQWEYVGGKVDASSGTITFNATHFSQ 1672 Query: 1547 YTVMIGSASFKDVADNHWAKKEINYLLTKGILE--QTAEFNPSGKVTRAQFANWISRAYG 1604 Y +F D+ DN WAK I L ++ I+E ++ P+ VTRA+F I R Sbjct: 1673 YAAFEYDKTFNDIKDN-WAKDVIEVLASRHIVEGMTDTQYEPNKTVTRAEFTAMILRLLN 1731 Query: 1605 LDGSGLTLPFSDVAAGSAGYNGVAAAYEAGIITGKSSTVFDPEATISRQEIATMLARALT 1664 + + FSDV +G N + AAY+AGII G P +I+R+E+ + RA Sbjct: 1732 IKDETYSGEFSDVKSGDWYANAIEAAYKAGIIEGDGKNA-RPNDSITREEMTAIAMRAYE 1790 Query: 1665 LYNGAKTVADPGAVNAAYTDGGKIAKWAAAGVALANRTDLFKGFEDGTFRPXXXXXXXXX 1724 + K + GA ++D I+ WA VA A + + G + F P Sbjct: 1791 MLTQYKE-ENIGA--TTFSDDKSISDWARNVVANAAKLGIVNGEPNNVFAPKGNATRAEA 1847 Query: 1725 XXLIYRLYQ 1733 +IY L + Sbjct: 1848 AAIIYGLLE 1856 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.130 0.357 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 2,324,390,229 Number of extensions: 97073185 Number of successful extensions: 204744 Number of sequences better than 1.0e-05: 78 Number of HSP's gapped: 204677 Number of HSP's successfully gapped: 79 Length of query: 1735 Length of database: 757,237,578 Length adjustment: 140 Effective length of query: 1595 Effective length of database: 377,123,718 Effective search space: 601512330210 Effective search space used: 601512330210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 133 (55.8 bits)