BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0231.0010.p (425 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_691508.1| hypothetical protein OB0587 [Oceanobacillus ihe... 278 2e-73 ref|NP_560186.1| conserved hypothetical protein [Pyrobaculum aer... 274 3e-72 ref|NP_819408.1| conserved hypothetical protein [Coxiella burnet... 273 8e-72 gb|EAI76015.1| unknown [environmental sequence] 258 1e-67 gb|EAI29220.1| unknown [environmental sequence] 258 2e-67 >ref|NP_691508.1| hypothetical protein OB0587 [Oceanobacillus iheyensis HTE831] dbj|BAC12543.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 354 Score = 278 bits (710), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 161/353 (45%), Positives = 217/353 (61%), Gaps = 23/353 (6%) Query: 67 TEXXXXNPENSQPALSSSAFPYTAQTLASGLNVPWEMAFAPDGRIFFTERPGTL-RLFEE 125 TE + ENS + S Q +AS L PW + + I+ TERPG + + E+ Sbjct: 18 TEQSDPSSENSSDVTTPSN---EQQVIASQLQTPWAIELVGE-TIYLTERPGNIVEVLED 73 Query: 126 GELRKNPLLELPAPFISEGEGGLLGFALDPAFEDNGFAYAYHSYRSASGGVENRVLRLSI 185 GEL + P+ E E E GLLG A F + AY Y+SY + +G + R+ +S Sbjct: 74 GELTRKPV-EFIDDLAGEPESGLLGIAFPDDFSSSNRAYIYYSYHTNNGNFQ-RITTISE 131 Query: 186 SGSKAEIDKVLLDGIPGDANHNGGRIKFGPDGYLYITTGEKYEPELAQDQESLGGKILRI 245 ++VLLDGIPG H+GGRIK+GPD LYIT G+ +PELAQ+ +SL GK+LR+ Sbjct: 132 QEDTWVEEEVLLDGIPGGTYHHGGRIKYGPDNLLYITVGDASQPELAQNLDSLAGKVLRM 191 Query: 246 SKDGSIPADNPFLDSPVYSRGHRNPQGLAWQPQSGVLYSSEHGQSSHDEINIIEAGSNYG 305 + DGSIP +NPF +S VYS GHRNPQGLAW + G LY++EHG S+ DEIN+IE G+NYG Sbjct: 192 NPDGSIPDNNPFENSYVYSYGHRNPQGLAWN-EKGELYATEHGNSALDEINLIEPGNNYG 250 Query: 306 WPLIEGDEAGTGRGEQMKLPLLHSGEETWAPSGMAFITQGPWSGELLVANLAGQQLLHVS 365 WP+IEGDE + M P++HSGE+TWAPSGM + +G L A L G+ + + Sbjct: 251 WPIIEGDET----MDDMVSPMIHSGEDTWAPSGMEYA-----NGALYFATLRGESVKRFN 301 Query: 366 PGTGDKKPSAEALFQGKWGRIRNVAEGPDGTLYVMTNNRDGRGSPGTADDQLI 418 P + E + + K GRIR+V G +G LY++TNN DGRG+P DDQL+ Sbjct: 302 PADSE----VETILENK-GRIRDVKYGNNG-LYIITNNTDGRGNPSDNDDQLL 348 >ref|NP_560186.1| conserved hypothetical protein [Pyrobaculum aerophilum] gb|AAL64368.1| conserved hypothetical protein [Pyrobaculum aerophilum str. IM2] Length = 371 Score = 274 bits (701), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 156/346 (45%), Positives = 207/346 (59%), Gaps = 19/346 (5%) Query: 83 SSAFPYTAQTLASGLNVPWEMAFAPDGRIFFTERPGTLRLFE-EGELRKNPLLELPAPF- 140 S + + +AS L VPW +A GR TERPG L L G+ +L A F Sbjct: 31 SEEWKFKISEVASDLEVPWSIAPLGGGRYLVTERPGRLVLISPSGK-------KLVASFD 83 Query: 141 -ISEGEGGLLGFALDPAFEDNGFAYAYHSYRSASGGVENRVLRLSISGSKAEIDKV--LL 197 + GE GLLG AL P F + Y Y SY + G + NRV+R + GS ++ +V L+ Sbjct: 84 VANVGEAGLLGLALHPEFPKKSWVYLYASYFAEGGHIRNRVIRGRLDGSTFKLKEVKTLI 143 Query: 198 DGIPGDANHNGGRIKFGPDGYLYITTGEKYEPELAQDQESLGGKILRISKDGSIPADNPF 257 DGIPG HNGGRI+FGPDG LYITTG+ +P LAQD SL GKILR+ ++G PADNPF Sbjct: 144 DGIPGAYIHNGGRIRFGPDGMLYITTGDAADPRLAQDLSSLAGKILRVDEEGRPPADNPF 203 Query: 258 LDSPVYSRGHRNPQGLAWQPQSGVLYSSEHGQSSHDEINIIEAGSNYGWPLIEGDEAGTG 317 +SP++S GHRNPQG+ W SGV+ ++EHG HDE+NII G NYGWPL G G Sbjct: 204 PNSPIWSYGHRNPQGIDWHRASGVMVATEHGPVGHDEVNIILKGGNYGWPLATGK---AG 260 Query: 318 RGEQMKLPLLHSGEETWAPSGMAFITQGPW---SGELLVANLAGQQLLHVSPGTGDKKPS 374 RGE + P++ +G ETWAPSG +F+ + G LL+A L G L V+ G + Sbjct: 261 RGEFVD-PVIDTGSETWAPSGASFVHGDMFPGLRGWLLIACLRGSMLAAVNFGDNMEVRK 319 Query: 375 AEALFQGKWGRIRNVAEGPDGTLYVMTNNRDGRGSPGTADDQLIAL 420 F+ +GR+R+V DG + + T+NRDGRGS DD+++ + Sbjct: 320 ISTFFKNVFGRLRDVVIDDDGGILISTSNRDGRGSLRAGDDKILKI 365 >ref|NP_819408.1| conserved hypothetical protein [Coxiella burnetii RSA 493] gb|AAO89922.1| conserved hypothetical protein [Coxiella burnetii RSA 493] Length = 369 Score = 273 bits (697), Expect = 8e-72, Method: Compositional matrix adjust. Identities = 151/336 (44%), Positives = 206/336 (61%), Gaps = 22/336 (6%) Query: 90 AQTLASGLNVPWEMAFAPDGRIFFTERPGTLRLFEEGELRKNPLLELPAPF---ISEGEG 146 L SGL VPW ++F PD + TER G + L + P++++ A S E Sbjct: 50 VSILISGLEVPWALSFLPDRDLLVTERKGRVLLIDLQS--GKPVIKVIAKINEVASVAES 107 Query: 147 GLLGFALDPAFEDNGFAYAYHSYRSASGGVENRVLRLSISGSKAEIDKVLLDGIPGDANH 206 GL G A+ P F+ N + Y Y++YR +G N+V+R + ++ K++LD I H Sbjct: 108 GLSGIAVHPHFQQNHWIYLYYTYRR-NGRYVNKVVRYELMNNQLTNPKIILDNILAARTH 166 Query: 207 NGGRIKFGPDGYLYITTGEKYEPELAQDQESLGGKILRISKDGSIPADNPFLDSPVYSRG 266 +GGRIKFGPDGYLYITTG+ + AQ+ +SL GKILRI DGSIPADNPF SPVYS G Sbjct: 167 DGGRIKFGPDGYLYITTGDAQVAKAAQNPKSLAGKILRIKDDGSIPADNPFPGSPVYSYG 226 Query: 267 HRNPQGLAWQPQSGVLYSSEHGQSSHDEINIIEAGSNYGWPLIEGDEAGTGRGEQMKLPL 326 HRN QG+AW ++G L+S+EHG +HDEIN+I+ G NYGWP++ G+E + M+ P+ Sbjct: 227 HRNSQGIAWD-KAGNLWSTEHGSDAHDEINLIKPGKNYGWPIVRGNE----KRSDMESPI 281 Query: 327 LHSGEETWAPSGMAFITQGPWSGELLVANLAGQQLLHVSPGTGDKKPSAEALFQGKWGRI 386 L S E TWAP+G A I + +L A L G+ L + T D F+G++GR+ Sbjct: 282 LQSCEATWAPAGAAIIGE-----KLYFAGLRGEALYVFNIKTHD----LHKYFEGEFGRL 332 Query: 387 RNVAEGPDGTLYVMTNNRDGRGSPGTADDQLIALKP 422 R V GPDG LYV T+NRDGRG P D+++ + P Sbjct: 333 RAVVVGPDGLLYVTTSNRDGRGLP--RPDEILVINP 366 >gb|EAI76015.1| unknown [environmental sequence] Length = 368 Score = 258 bits (660), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 146/324 (45%), Positives = 187/324 (57%), Gaps = 19/324 (5%) Query: 91 QTLASGLNVPWEMAFAPDGRIFFTERPGTLRLFEE-GELRKNPLLELPAPFISEGEGGLL 149 + GLN PWEM FAP+G I+F+ER G + E GE + + EGG L Sbjct: 4 DVVVDGLNNPWEMVFAPNGDIYFSERDGRVWKIENFGEAKVIQTFPKSGSY----EGGTL 59 Query: 150 GFALDPAFEDNGFAYAYHSYRSASGGVENRVLRLSISGSKAEIDKVLLDGIPGDANHNGG 209 G ALDP FE+N Y Y + +N+V ++ G + + ++D IPG H+GG Sbjct: 60 GLALDPNFEENKKIYIYQTNLELEF-FQNKVFSFTVDGDELVDVQTVIDDIPGAPWHDGG 118 Query: 210 RIKFGPDGYLYITTGEKYEPELAQDQESLGGKILRISKDGSIPADNPFLDSPVYSRGHRN 269 RI FGPD LYITTG+ P +QD SL GKILRI+ DGSIP DNPF S ++S GHRN Sbjct: 119 RIAFGPDEKLYITTGDAVNPGWSQDLSSLAGKILRINSDGSIPDDNPFDSSAIFSYGHRN 178 Query: 270 PQGLAWQPQSGVLYSSEHGQS-----SHDEINIIEAGSNYGWPLIEGDEAGTGRGEQMKL 324 PQG+AW + G+L SSEHG S HDEIN+I G NYGWP + GD + + Sbjct: 179 PQGIAWNDE-GMLVSSEHGPSGEMGYGHDEINVIVKGKNYGWPKVVGDSS----DDSFVN 233 Query: 325 PLLHSGEETWAPSGMAFITQGPWS---GELLVANLAGQQLLHVSPGTGDKKPSAEALFQG 381 P++HSGE+TWAPSGM F S G+ LV L GQ L+ + SAE +F+G Sbjct: 234 PIIHSGEQTWAPSGMVFYNSDKISSLEGKFLVGALRGQHLMVLDVANDGSLISAEKMFEG 293 Query: 382 KWGRIRNVAEGPDGTLYVMTNNRD 405 +GRIR PDG LY++T N D Sbjct: 294 DFGRIRTAQISPDGVLYLLTANGD 317 >gb|EAI29220.1| unknown [environmental sequence] Length = 367 Score = 258 bits (659), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 146/324 (45%), Positives = 187/324 (57%), Gaps = 19/324 (5%) Query: 91 QTLASGLNVPWEMAFAPDGRIFFTERPGTLRLFEE-GELRKNPLLELPAPFISEGEGGLL 149 + GLN PWEM FAP+G I+F+ER G + E GE + + EGG L Sbjct: 4 DVVVDGLNNPWEMVFAPNGDIYFSERDGRVWKIENFGEAKVIQTFPKSGSY----EGGTL 59 Query: 150 GFALDPAFEDNGFAYAYHSYRSASGGVENRVLRLSISGSKAEIDKVLLDGIPGDANHNGG 209 G ALDP FE+N Y Y + +N+V ++ G + + ++DGIPG H+GG Sbjct: 60 GLALDPNFEENKKIYIYQTNLELEF-FQNKVFSFTVDGDELIDVQTVIDGIPGAPWHDGG 118 Query: 210 RIKFGPDGYLYITTGEKYEPELAQDQESLGGKILRISKDGSIPADNPFLDSPVYSRGHRN 269 RI FGPD LYITTG+ P +QD S GKILRI+ DGSIP DNPF S ++S GHRN Sbjct: 119 RIAFGPDDKLYITTGDAVNPGWSQDLSSPAGKILRINSDGSIPDDNPFDSSAIFSYGHRN 178 Query: 270 PQGLAWQPQSGVLYSSEHGQS-----SHDEINIIEAGSNYGWPLIEGDEAGTGRGEQMKL 324 PQG+AW +G+L SSEHG S HDEIN+I G NYGWP + GD + + Sbjct: 179 PQGIAWN-DNGLLVSSEHGPSGEMGYGHDEINVIVKGKNYGWPKVVGDSS----DDSFVN 233 Query: 325 PLLHSGEETWAPSGMAFITQGPWS---GELLVANLAGQQLLHVSPGTGDKKPSAEALFQG 381 P++HSGE+TWAPSGM F S G+ LV L GQ L+ + SAE +F+G Sbjct: 234 PIIHSGEQTWAPSGMVFYNSDKISSLEGKFLVGALRGQHLMVLDIANDSSLISAEKMFEG 293 Query: 382 KWGRIRNVAEGPDGTLYVMTNNRD 405 +GRIR PDG LY++T N D Sbjct: 294 DFGRIRTAQISPDGVLYLLTANGD 317 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.134 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 720,317,076 Number of extensions: 34102749 Number of successful extensions: 63391 Number of sequences better than 1.0e-05: 553 Number of HSP's gapped: 62275 Number of HSP's successfully gapped: 553 Length of query: 425 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 297 Effective length of database: 409,704,906 Effective search space: 121682357082 Effective search space used: 121682357082 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 127 (53.5 bits)