BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0229.0004.p (358 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_241929.1| D-mannonate hydrolase [Bacillus halodurans] >gi... 487 e-136 sp|Q9ZFL7|UXUA_BACST Mannonate dehydratase (D-mannonate hydrolas... 449 e-125 ref|NP_389116.1| similar to D-mannonate hydrolase [Bacillus subt... 438 e-121 ref|NP_227885.1| D-mannonate hydrolase [Thermotoga maritima] >gi... 437 e-121 emb|CAE11234.1| UxuA protein [Bacillus amyloliquefaciens] 435 e-121 >ref|NP_241929.1| D-mannonate hydrolase [Bacillus halodurans] sp|Q9KDZ8|UXA1_BACHD Mannonate dehydratase 1 (D-mannonate hydrolase 1) pir||G83782 D-mannonate hydrolase BH1063 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04782.1| D-mannonate hydrolase [Bacillus halodurans] Length = 361 Score = 487 bits (1253), Expect = e-136, Method: Compositional matrix adjust. Identities = 227/354 (64%), Positives = 276/354 (77%), Gaps = 4/354 (1%) Query: 1 MNMTWRWYGEGNDNITLDHIRQIPGVMGIVWSLHEKVAGEVWEMERIQEVADQITSKGFS 60 M M +RW+G GND+++L HIRQIPGV GIVW+LH+ GEVW + RI E+ +Q F Sbjct: 1 MRMVFRWFGAGNDSVSLAHIRQIPGVEGIVWALHDIPPGEVWPLSRIMEIKEQAEQHQFH 60 Query: 61 TAVVESVNVHDDIKIGLPTRDKYIDIYIDTIRKLAKVGVKVICYNFMPVFDWTRTELYKE 120 VVESVNVH+DIK+GLPTRD+YI+ Y TI+ LAK GVKVICYNFMPVFDW RT+LYK+ Sbjct: 61 IDVVESVNVHEDIKLGLPTRDRYIEAYKQTIKHLAKAGVKVICYNFMPVFDWVRTDLYKK 120 Query: 121 LPDGSNALFYEKAAITD-NPREMVDRILKGAGQFTMPGWEPERLAKLDELFAAYADVTEE 179 L DGS ALFYEK + + NP+ +V+ I FTMPGWEPERLA L LF AY V+ E Sbjct: 121 LEDGSTALFYEKKEVDEMNPKGLVETIASNK-TFTMPGWEPERLASLKTLFEAYEHVSTE 179 Query: 180 KLFENLKYFLERIIPVCEEVDIKMAIHPDDPAWPIFGLPRIIRSRDTIRRMLDAVDSPYN 239 L+++L YFL+ +IPV E DIKMAIHPDDP WPIFGLPRI+ + D IRR+L VD P N Sbjct: 180 DLWDHLHYFLQEVIPVAEACDIKMAIHPDDPPWPIFGLPRIVTNHDNIRRLLKLVDHPTN 239 Query: 240 GLTFCTGSLGSNPENDLPAMIREFHDRIYFAHIRNVKVFENGDFIEVSHRGRDGSVDVAE 299 G+T C+G+LG++P N +P MIREF DRI FAHIRN+K F+NGDF+E SHR +DGS+D+ Sbjct: 240 GITLCSGALGADPNNQVPEMIREFADRIPFAHIRNIKRFDNGDFVETSHREQDGSIDIVS 299 Query: 300 VVKAYHENGYTGYVRPDHGRHLWGEEKNCRPGYGLYDRALGIMYLLGVWDSLDN 353 +VKAYHE G+TGYVRPDHGRH+W E+ CRPGYGLYDRALGIMYL G+WDS+ N Sbjct: 300 IVKAYHEVGFTGYVRPDHGRHIWDEQ--CRPGYGLYDRALGIMYLWGIWDSVQN 351 >sp|Q9ZFL7|UXUA_BACST Mannonate dehydratase (D-mannonate hydrolase) gb|AAC98134.1| D-mannonate hydrolase [Geobacillus stearothermophilus] Length = 371 Score = 449 bits (1154), Expect = e-125, Method: Compositional matrix adjust. Identities = 209/358 (58%), Positives = 270/358 (75%), Gaps = 5/358 (1%) Query: 1 MNMTWRWYGEGNDNITLDHIRQIPGVMGIVWSLHEKVAGEVWEMERIQEVADQITSKGFS 60 M MT+RW+G+ +D ++LDHIRQIPGV GIV +L+ GEVW ++ I E+ Q+ KGF Sbjct: 1 MKMTFRWFGKEHDTVSLDHIRQIPGVEGIVGALYHIPVGEVWPLDDILELKRQVNEKGFH 60 Query: 61 TAVVESVNVHDDIKIGLPTRDKYIDIYIDTIRKLAKVGVKVICYNFMPVFDWTRTELYKE 120 V+ESVNVH+DIK+GLP+RD+YI+ Y TIR LAK GVKVICYNFMP+FDWTR++L K Sbjct: 61 LEVIESVNVHEDIKLGLPSRDRYIENYKQTIRNLAKAGVKVICYNFMPIFDWTRSDLAKR 120 Query: 121 LPDGSNALFYEKAAITD-NPREMVDRILKGAGQFTMPGWEPERLAKLDELFAAYADVTEE 179 PDGS L YEK I +P EM+ RI GA F +PGWEPERL + LF+ Y VTEE Sbjct: 121 RPDGSTVLAYEKQKIEQIDPEEMIRRIESGANGFLLPGWEPERLKTIKPLFSLYKGVTEE 180 Query: 180 KLFENLKYFLERIIPVCEEVDIKMAIHPDDPAWPIFGLPRIIRSRDTIRRMLDAVDSPYN 239 LF++L+YFLE+I+PV EE ++MA+HPDDP W +FGLPRI +++ + R++ V+SP N Sbjct: 181 DLFDHLRYFLEQIVPVAEECGVRMALHPDDPPWSVFGLPRIATNKENLDRIVHMVNSPAN 240 Query: 240 GLTFCTGSLGSNPENDLPAMIREF--HDRIYFAHIRNVKVFENGDFIEVSHRGRDGSVDV 297 GLT C+GSLG+NP ND+P + R F R+ FAH+RNV++ NGDF E SHR DGS+++ Sbjct: 241 GLTLCSGSLGANPANDVPDIFRHFLRMGRVPFAHVRNVEIHANGDFEETSHRSCDGSLNI 300 Query: 298 AEVVKAYHENGYTGYVRPDHGRHLWGEEKNCRPGYGLYDRALGIMYLLGVWDSLDNVK 355 E++KA HE + GY+RPDHGR +WGE+ RPGYGLYDRALGIMYLLG+WDSL+N K Sbjct: 301 CEIMKALHEANFQGYIRPDHGRMIWGEQ--ARPGYGLYDRALGIMYLLGIWDSLENEK 356 >ref|NP_389116.1| similar to D-mannonate hydrolase [Bacillus subtilis] sp|O34346|UXUA_BACSU Mannonate dehydratase (D-mannonate hydrolase) pir||G69852 D-mannonate hydrolase homolog yjmE - Bacillus subtilis gb|AAC46330.1| hypothetical D-mannonate hydratase [Bacillus subtilis] emb|CAB13091.1| D-mannonate hydrolase [Bacillus subtilis subsp. subtilis str. 168] Length = 359 Score = 438 bits (1126), Expect = e-121, Method: Compositional matrix adjust. Identities = 203/353 (57%), Positives = 266/353 (75%), Gaps = 4/353 (1%) Query: 1 MNMTWRWYGEGNDNITLDHIRQIPGVMGIVWSLHEKVAGEVWEMERIQEVADQITSKGFS 60 MNMT+RWYG GND +TL++++QIPGV GIVW+LH+K G+VWE E I+ + I S GF Sbjct: 1 MNMTFRWYGRGNDTVTLEYVKQIPGVKGIVWALHQKPVGDVWEKEEIRAETEYIQSYGFH 60 Query: 61 TAVVESVNVHDDIKIGLPTRDKYIDIYIDTIRKLAKVGVKVICYNFMPVFDWTRTELYKE 120 VVESVNVH+ IK+G R +YI+ Y TIR LA GVKVICYNFMPVFDWTRT++++ Sbjct: 61 AEVVESVNVHEAIKLGNEERGRYIENYKQTIRNLAGFGVKVICYNFMPVFDWTRTDMFRP 120 Query: 121 LPDGSNALFYEKAAITD-NPREMVDRILKGAGQFTMPGWEPERLAKLDELFAAYADVTEE 179 L DGS ALF+EKA + +P+E++ R ++ A T+PGWEPE+LA++ ELFAAY V EE Sbjct: 121 LEDGSTALFFEKAKVESLDPQELI-RTVEEASDMTLPGWEPEKLARIKELFAAYRTVDEE 179 Query: 180 KLFENLKYFLERIIPVCEEVDIKMAIHPDDPAWPIFGLPRIIRSRDTIRRMLDAVDSPYN 239 KL++NL +FL+ I+PV E ++MAIHPDDP WPIFGLPRII + +++ DSP N Sbjct: 180 KLWDNLSFFLQEILPVAEAYGVQMAIHPDDPPWPIFGLPRIITGEASYKKLRAISDSPSN 239 Query: 240 GLTFCTGSLGSNPENDLPAMIREFHDRIYFAHIRNVKVFENGDFIEVSHRGRDGSVDVAE 299 +T CTGS+G+NP ND+ + + + F+HIRNVK++ENGDFIE SH +DGS+++ Sbjct: 240 CITLCTGSMGANPANDMVEIAKTYAGIAPFSHIRNVKIYENGDFIETSHLTKDGSINIQG 299 Query: 300 VVKAYHENGYTGYVRPDHGRHLWGEEKNCRPGYGLYDRALGIMYLLGVWDSLD 352 V++ H+ Y GYVRPDHGRHLWGE+ CRPGYGLYDRALGIMYL G+WD+ + Sbjct: 300 VMEELHKQDYEGYVRPDHGRHLWGEQ--CRPGYGLYDRALGIMYLNGLWDAYE 350 >ref|NP_227885.1| D-mannonate hydrolase [Thermotoga maritima] sp|Q9WXS4|UXUA_THEMA Mannonate dehydratase (D-mannonate hydrolase) pir||A72423 D-mannonate hydrolase - Thermotoga maritima (strain MSB8) gb|AAD35163.1| D-mannonate hydrolase [Thermotoga maritima] Length = 360 Score = 437 bits (1125), Expect = e-121, Method: Compositional matrix adjust. Identities = 208/358 (58%), Positives = 266/358 (74%), Gaps = 5/358 (1%) Query: 1 MNMTWRWYGEGNDNITLDHIRQIPGVMGIVWSLHEKVAGEVWEMERIQEVADQITSKGFS 60 M + +RWYGE +D +TL+ IRQIPGV G+V +L + GEVW E I ++ + + G Sbjct: 1 MKLVFRWYGEKHDTVTLEQIRQIPGVEGVVGALFDIPVGEVWPFEEIMKLKETVEKAGLK 60 Query: 61 TAVVESVNVHDDIKIGLPTRDKYIDIYIDTIRKLAKVGVKVICYNFMPVFDWTRTELYKE 120 V+ESVNVH+DIK+GLPTRD+YI+ Y TIR LAK GVKV+CYNFMPVFDW RT+L+K+ Sbjct: 61 LEVIESVNVHEDIKLGLPTRDRYIENYKKTIRNLAKAGVKVVCYNFMPVFDWMRTDLHKK 120 Query: 121 LPDGSNALFYEKAAITD-NPREMVDRILKGAGQFTMPGWEPERLAKLDELFAAYADVTEE 179 LPDGS + Y+ I P E++ R+ +G+ F +PGWE +RL KL E F Y +V EE Sbjct: 121 LPDGSETMEYDHRLIEGVTPDELIKRVKEGSQGFVLPGWEWDRLEKLRETFELYKNVDEE 180 Query: 180 KLFENLKYFLERIIPVCEEVDIKMAIHPDDPAWPIFGLPRIIRSRDTIRRMLDAVDSPYN 239 KLFENL YFLER+IPVCEE D+K+AIHPDDP W IFGLPRII +++ I RML AVDSPYN Sbjct: 181 KLFENLVYFLERVIPVCEECDVKLAIHPDDPPWSIFGLPRIITNKENIERMLKAVDSPYN 240 Query: 240 GLTFCTGSLGSNPENDLPAMIREFHD--RIYFAHIRNVKVFENGDFIEVSHRGRDGSVDV 297 G+TFC GSLG+NPEN++P MIR F RI+FAH+RN+K F E +H GS D+ Sbjct: 241 GITFCMGSLGANPENNIPEMIRYFGKMGRIHFAHVRNLKFTGEKSFYETAHPSFCGSHDL 300 Query: 298 AEVVKAYHENGYTGYVRPDHGRHLWGEEKNCRPGYGLYDRALGIMYLLGVWDSLDNVK 355 EV+KA+H+ GY GY+RPDHGR +WGE+ RPGYGLYDRALG Y+LG+W+++D +K Sbjct: 301 FEVMKAFHDIGYEGYIRPDHGRLIWGEK--ARPGYGLYDRALGATYILGLWEAIDKMK 356 >emb|CAE11234.1| UxuA protein [Bacillus amyloliquefaciens] Length = 380 Score = 435 bits (1118), Expect = e-121, Method: Compositional matrix adjust. Identities = 200/353 (56%), Positives = 259/353 (73%), Gaps = 2/353 (0%) Query: 1 MNMTWRWYGEGNDNITLDHIRQIPGVMGIVWSLHEKVAGEVWEMERIQEVADQITSKGFS 60 MN+T+RWYG+GND +TL+H+RQIPGV GIVW+LH+K AGEVWE E I+ I S GF Sbjct: 23 MNITFRWYGQGNDTVTLEHVRQIPGVKGIVWALHQKPAGEVWEKEDIEAEVRFIQSYGFH 82 Query: 61 TAVVESVNVHDDIKIGLPTRDKYIDIYIDTIRKLAKVGVKVICYNFMPVFDWTRTELYKE 120 VVESVNVH+ IK+G R +YI+ Y TIR L++ GVKVICYNFMP+FDWTRT++++ Sbjct: 83 AEVVESVNVHEAIKLGHEERGQYIEHYKQTIRNLSEFGVKVICYNFMPIFDWTRTDMFRA 142 Query: 121 LPDGSNALFYEKAAITDNPREMVDRILKGAGQFTMPGWEPERLAKLDELFAAYADVTEEK 180 L DGS ALF+EKA + P E + R ++ + T+PGWEPE++A++ ELF AY V E++ Sbjct: 143 LEDGSTALFFEKAKVESLPPEELIRTVEESSDLTLPGWEPEKMARIKELFEAYQTVEEKQ 202 Query: 181 LFENLKYFLERIIPVCEEVDIKMAIHPDDPAWPIFGLPRIIRSRDTIRRMLDAVDSPYNG 240 L+ NL++FL I+PV EE I+MAIHPDDP W IFGLPRII + ++ + DSP N Sbjct: 203 LWANLEFFLHEILPVAEEHGIQMAIHPDDPPWSIFGLPRIITGEKSYEKLREISDSPANC 262 Query: 241 LTFCTGSLGSNPENDLPAMIREFHDRIYFAHIRNVKVFENGDFIEVSHRGRDGSVDVAEV 300 +T CTGS+G++P ND+ + + + FAHIRNVK+ +NGDF E SH DGS+D+ V Sbjct: 263 ITLCTGSMGADPANDMVKITKHYAGAAPFAHIRNVKICDNGDFTETSHLTSDGSIDITGV 322 Query: 301 VKAYHENGYTGYVRPDHGRHLWGEEKNCRPGYGLYDRALGIMYLLGVWDSLDN 353 VK H Y GYVRPDHGRH+WGE+ CRPGYGLYDRALGIMYL G+WD+ + Sbjct: 323 VKELHGQDYKGYVRPDHGRHIWGEQ--CRPGYGLYDRALGIMYLNGLWDAFSS 373 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.141 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 587,250,948 Number of extensions: 25644364 Number of successful extensions: 57235 Number of sequences better than 1.0e-05: 94 Number of HSP's gapped: 57094 Number of HSP's successfully gapped: 120 Length of query: 358 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 231 Effective length of database: 412,420,005 Effective search space: 95269021155 Effective search space used: 95269021155 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)