BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0225.0015.p (310 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAC62422.1| ABC transporter ATP-binding protein [Bacillus fir... 141 2e-32 ref|NP_561360.1| probable ABC transporter [Clostridium perfringe... 131 3e-29 ref|NP_646691.1| ORFID:MW1874~hypothetical protein, similar to A... 127 5e-28 ref|NP_372457.1| hypothetical protein [Staphylococcus aureus sub... 124 2e-27 ref|NP_846672.1| ABC transporter, ATP-binding protein [Bacillus ... 124 4e-27 >gb|AAC62422.1| ABC transporter ATP-binding protein [Bacillus firmus] Length = 290 Score = 141 bits (356), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 88/290 (30%), Positives = 147/290 (50%), Gaps = 11/290 (3%) Query: 6 IELRNVSKKRRQGKTIGPLNLNLPQGYITALVGQNGSGKSTMLHMLLQLTFPDEGEIRWF 65 I L+++ KK + +GPL+ + G I ALVG NG+GKST+ +++L + GEI Sbjct: 2 ILLQDIYKKVDKSFELGPLSCQIEPGSIVALVGNNGAGKSTLFQTIMELVKKESGEI--- 58 Query: 66 EHSYADGLPLALRQRIAYVPENPQAEEKYWSAAEAAEFRRHWYPAWDQDFFEALLAKFEV 125 ++ + +Q AYVP+ P + + A+ A+F YP W+++ F+ L + F++ Sbjct: 59 DYHFGTDRENGWKQAFAYVPQTPLMYHGF-TIAQLADFFEMSYPGWNRNEFDRLTSLFDL 117 Query: 126 PEDAKLGKMSKGERRKFEIXXXXXXXXXXXXXDEPSSGLDPFAWKAMIETLRKYMDES-G 184 P ++ K+S G ++K + DEP +G+D + M + + +YM+ + Sbjct: 118 PLKKQVEKLSGGMQKKAMLSLTLARQSKVLLLDEPLAGVDFEGQEQMRDEMVRYMERAED 177 Query: 185 ATVVISTHIVEEVRRLADYIVLMHHGQLLGMAEKDSLFGSWSEIWXXXXXXXXXXXXXXX 244 T+++STH +E+R LADYI+LM G LL EKDSL SWS IW Sbjct: 178 QTILLSTHSADEIRTLADYILLMKEGHLLNQYEKDSLIASWSRIWVEGDTSALKNLKGMV 237 Query: 245 XXXXXHFAIDTPGVASFITPQAQQNEKRVQDLGVKVIKSRILELDEILSL 294 ++ +T A + E+ ++ +G V + LEL EIL + Sbjct: 238 STSKQGSLVEC------VTSNAFEVEQELKKMGATVTSVQPLELREILRI 281 >ref|NP_561360.1| probable ABC transporter [Clostridium perfringens] dbj|BAB80150.1| probable ABC transporter [Clostridium perfringens str. 13] Length = 287 Score = 131 bits (329), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 66/224 (29%), Positives = 126/224 (56%), Gaps = 3/224 (1%) Query: 2 VQQAIELRNVSKKRRQGKTIGPLNLNLPQGYITALVGQNGSGKSTMLHMLLQLTFPDEGE 61 ++ +E++N+SK + G ++ ++ N+P+G++ L+G NG+GK+T + ++ L D GE Sbjct: 1 MENVLEIKNISKNYK-GFSLKDVSFNIPKGFVMGLIGPNGAGKTTTIKAIMNLINLDSGE 59 Query: 62 IRWFEHSYADGLPLALRQRIAYVPENPQAEEKYWSAAEAAEFRRHWYPAWDQDFFEALLA 121 I F ++++ RI +V ++ A E + + E + +Y WD++ F+ L Sbjct: 60 IYIFGEDIKKN-EISIKDRIGFVYDDCCAFEDF-TIKENKKIISKFYSKWDEEKFKYYLE 117 Query: 122 KFEVPEDAKLGKMSKGERRKFEIXXXXXXXXXXXXXDEPSSGLDPFAWKAMIETLRKYMD 181 KFE+ E+ KL ++SKG++ +F + DEP+SGLDP +++ L + + Sbjct: 118 KFELNENKKLKQLSKGQKMRFSLALALSHKAELLILDEPTSGLDPVFRSELLDILFEIIG 177 Query: 182 ESGATVVISTHIVEEVRRLADYIVLMHHGQLLGMAEKDSLFGSW 225 E +++ STHI ++ +LADYI ++ G++ EKD L + Sbjct: 178 EEEVSILYSTHITTDLEKLADYITFLNKGKVEFSLEKDLLLEEY 221 >ref|NP_646691.1| ORFID:MW1874~hypothetical protein, similar to ABC transporter, ATP-binding protein [Staphylococcus aureus subsp. aureus MW2] dbj|BAB95739.1| MW1874 [Staphylococcus aureus subsp. aureus MW2] Length = 298 Score = 127 bits (318), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 65/222 (29%), Positives = 117/222 (52%), Gaps = 3/222 (1%) Query: 4 QAIELRNVSKKRRQGKTIGPLNLNLPQGYITALVGQNGSGKSTMLHMLLQLTFPDEGEIR 63 AIEL NV+ Q + ++ +PQG++T +G+NG+GK+T++ +++ L P G IR Sbjct: 2 NAIELSNVNYSSDQF-NLKNISFKVPQGFVTGFIGRNGAGKTTIIRLIMDLYQPQTGVIR 60 Query: 64 WFEHSYADGLPLALRQRIAYVPENPQAEEKYWSAAEAAEFRRHWYPAWDQDFFEALLAKF 123 E A P+ L+ RI +V E+ W+ + + +Y WD FE L KF Sbjct: 61 VLEEDMALN-PIELKNRIGFVYSENYFNER-WTTKQLEKMIAPFYRKWDHQVFEFYLEKF 118 Query: 124 EVPEDAKLGKMSKGERRKFEIXXXXXXXXXXXXXDEPSSGLDPFAWKAMIETLRKYMDES 183 ++P + + S G + K + DEP+SGLDP A ++E +++ + + Sbjct: 119 DLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLDPLARNELLEIIQQELIDE 178 Query: 184 GATVVISTHIVEEVRRLADYIVLMHHGQLLGMAEKDSLFGSW 225 T+ +STHI+ ++ ++ADYI+ + G+++ K+ L + Sbjct: 179 NKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQRY 220 >ref|NP_372457.1| hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] ref|NP_375040.1| hypothetical protein, similar to ABC transporter, ATP-binding protein [Staphylococcus aureus subsp. aureus N315] pir||D89982 hypothetical protein SA1747 [imported] - Staphylococcus aureus (strain N315) dbj|BAB43019.1| SA1747 [Staphylococcus aureus subsp. aureus N315] dbj|BAB58095.1| hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] Length = 298 Score = 124 bits (311), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 64/222 (28%), Positives = 116/222 (52%), Gaps = 3/222 (1%) Query: 4 QAIELRNVSKKRRQGKTIGPLNLNLPQGYITALVGQNGSGKSTMLHMLLQLTFPDEGEIR 63 AIEL NV+ Q + ++ +PQG++T +G+NG+GK+T++ +++ L P G IR Sbjct: 2 NAIELSNVNYSSDQF-NLKNISFKVPQGFVTGFIGRNGAGKTTIIRLIMDLYQPQTGVIR 60 Query: 64 WFEHSYADGLPLALRQRIAYVPENPQAEEKYWSAAEAAEFRRHWYPAWDQDFFEALLAKF 123 E A P+ L+ I +V E+ W+ + + +Y WD FE L KF Sbjct: 61 VLEEDMALN-PIELKNSIGFVYSENYFNER-WTTKQLEKMIAPFYRKWDHQVFEFYLEKF 118 Query: 124 EVPEDAKLGKMSKGERRKFEIXXXXXXXXXXXXXDEPSSGLDPFAWKAMIETLRKYMDES 183 ++P + + S G + K + DEP+SGLDP A ++E +++ + + Sbjct: 119 DLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLDPLARNELLEIIQQELIDE 178 Query: 184 GATVVISTHIVEEVRRLADYIVLMHHGQLLGMAEKDSLFGSW 225 T+ +STHI+ ++ ++ADYI+ + G+++ K+ L + Sbjct: 179 NKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQRY 220 >ref|NP_846672.1| ABC transporter, ATP-binding protein [Bacillus anthracis str. Ames] gb|AAP28158.1| ABC transporter, ATP-binding protein [Bacillus anthracis str. Ames] Length = 290 Score = 124 bits (310), Expect = 4e-27, Method: Compositional matrix adjust. Identities = 67/223 (30%), Positives = 119/223 (53%), Gaps = 9/223 (4%) Query: 6 IELRNVSKKRRQGKTIGPLNLNLPQGYITALVGQNGSGKSTMLHMLLQLTFPDEGEIRWF 65 +EL+NV K + + ++ LP+GYI VG NG+GKST + M++ L D GEI+ F Sbjct: 2 LELKNVCK-SYEDFAVKNISFTLPRGYIMGFVGPNGAGKSTTIKMIMNLIKKDSGEIKIF 60 Query: 66 EHSYADGLPLALRQRIAYVPENPQAEEKYWSAAEAAEFRR---HWYPAWDQDFFEALLAK 122 ++Q I +V + E Y+ + +R Y WD++ +++ + + Sbjct: 61 GKDNKKA-EKEIKQNIGFVYD----ENHYYEDLTCEQMKRIIAPLYKKWDEEQYQSYMHR 115 Query: 123 FEVPEDAKLGKMSKGERRKFEIXXXXXXXXXXXXXDEPSSGLDPFAWKAMIETLRKYMDE 182 +VP+ K+ ++SKG + KF I DEP++GLDP +++ L++ + E Sbjct: 116 LQVPKYKKIKELSKGMKMKFAIAIALSHHAEFIIMDEPTAGLDPVVRSELLDMLQEIVME 175 Query: 183 SGATVVISTHIVEEVRRLADYIVLMHHGQLLGMAEKDSLFGSW 225 +V+ STHI ++ R+ADYI ++ G+++ EKD L ++ Sbjct: 176 DEVSVLFSTHITTDLERIADYITFINDGEIIFTGEKDDLMENY 218 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 412,800,797 Number of extensions: 15108226 Number of successful extensions: 98255 Number of sequences better than 1.0e-05: 3772 Number of HSP's gapped: 97897 Number of HSP's successfully gapped: 3824 Length of query: 310 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 185 Effective length of database: 417,850,203 Effective search space: 77302287555 Effective search space used: 77302287555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)