BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0006.p (349 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_654629.1| hypothetical protein predicted by GeneMark [Bac... 208 1e-52 ref|NP_830487.1| Permease [Bacillus cereus ATCC 14579] >gi|29894... 208 2e-52 ref|NP_977073.1| membrane protein, putative [Bacillus cereus ATC... 203 4e-51 ref|NP_782003.1| putative permease [Clostridium tetani E88] >gi|... 172 1e-41 ref|ZP_00036729.1| COG4767: Glycopeptide antibiotics resistance ... 167 3e-40 >ref|NP_654629.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_843208.1| membrane protein, putative [Bacillus anthracis str. Ames] gb|AAP24694.1| membrane protein, putative [Bacillus anthracis str. Ames] Length = 383 Score = 208 bits (530), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 127/358 (35%), Positives = 200/358 (55%), Gaps = 18/358 (5%) Query: 4 SYLFPISNAFMSFPVAALVFTLPFLVVQYRRHGYINKVRAVVXXXXXXXXXNAFYLIMLP 63 +YLFP+ AF+ FP A+ +PFL+ YR++GY+NK R+ + NA++L++LP Sbjct: 3 AYLFPVKTAFILFPFLAMFLLIPFLIFNYRKYGYLNKWRSFILYSLLLYLLNAYFLVILP 62 Query: 64 MPASRHNAAPVGSIIQHV---PLQFIQEIRRNSNIDPGNFLSYFSIFRQPDFLLAAFNVV 120 +P + + + QH+ P FIQEI +++ +YF + ++ FL AFNV+ Sbjct: 63 LPQTYDTCSLQPANTQHMQLSPFYFIQEISNHTSAILTKPATYFYLLKESAFLQVAFNVL 122 Query: 121 LTVPFGLFLGYYFRTRWVMCILLSFGLSLLFEVTQITGIYGFFDHPYRIFDVDDLITNTL 180 LTVPFG++L YYFR ++ I +SF LSL FE+TQ+TG+YG ++ YR+FD+DDL NTL Sbjct: 123 LTVPFGIYLRYYFRRSFLQTICISFCLSLFFELTQVTGLYGIYNCAYRLFDIDDLFLNTL 182 Query: 181 GGVLGYRIALWISALLPRIEQLDSKVDYSAKRVTYTRRGIAWMLDLPIWMIPFLTLHSWG 240 GGV+G+ IA LP+ +LDS VD K V + RR IA +D I + + G Sbjct: 183 GGVIGFIIAPIFMYFLPKANELDSHVDLETKPVGFVRRFIAMQIDWIFLSIVVPVIKNKG 242 Query: 241 MQ------GAY-------WITTA--VYFVLLPCCTGGRTFGKWVVRISISGQDGRSRLWG 285 AY +IT + +YF+++P T G+T GK ++RI + G+ R + Sbjct: 243 NSFFVSNIQAYTNIYELIFITCSILIYFIIIPYFTNGKTIGKALLRIYVKGKSDRITMKE 302 Query: 286 LIVRYVLQYGVLIGANVFXXXXXXXXXXXXXWTGIPYSLVMLLDFMFLIHLIIRVFKR 343 L +RY + Y +L G N + ++++ +F+IH+++ VF R Sbjct: 303 LFIRYGIFYFILGGINYILSSSSILNLKEPLVLIVIVLFQLVINGLFIIHVLLHVFSR 360 >ref|NP_830487.1| Permease [Bacillus cereus ATCC 14579] gb|AAP07688.1| Permease [Bacillus cereus ATCC 14579] Length = 383 Score = 208 bits (530), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 129/361 (35%), Positives = 203/361 (56%), Gaps = 24/361 (6%) Query: 4 SYLFPISNAFMSFPVAALVFTLPFLVVQYRRHGYINKVRAVVXXXXXXXXXNAFYLIMLP 63 +YLFP+ AF+ FP A+ +PFL+ YR++GY+NK R+ + NA++L++LP Sbjct: 3 AYLFPVKTAFILFPFLAMFLLIPFLIFNYRKYGYLNKWRSFILYSLLLYLLNAYFLVILP 62 Query: 64 MPASRHNAAPVGSIIQHV---PLQFIQEIRRNSNIDPGNFLSYFSIFRQPDFLLAAFNVV 120 +P + + + QH+ P FIQEI +++ +YF + ++ FL AFNV+ Sbjct: 63 LPQTFDTCSLQPANTQHMQLSPFYFIQEISSHTSAVLTKPATYFYLLKESAFLQVAFNVL 122 Query: 121 LTVPFGLFLGYYFRTRWVMCILLSFGLSLLFEVTQITGIYGFFDHPYRIFDVDDLITNTL 180 LTVPFG++L YYFR ++ I +SF LSL FE+TQ+TG+YG ++ YR+FD+DDL NTL Sbjct: 123 LTVPFGIYLRYYFRRSFLQTICISFFLSLFFELTQVTGLYGIYNCAYRLFDIDDLFLNTL 182 Query: 181 GGVLGYRIALWISALLPRIEQLDSKVDYSAKRVTYTRRGIAWMLDLPIWM-----IPFL- 234 GGV+G+ IA + LP+ +LDS +D K V + RR IA +D W+ +P + Sbjct: 183 GGVIGFIIAPIFTYFLPKTNELDSHIDLETKPVGFIRRLIAMQID---WIFLSIVVPVVK 239 Query: 235 ---------TLHSW-GMQGAYWITTA--VYFVLLPCCTGGRTFGKWVVRISISGQDGRSR 282 + S+ M +IT + +YF+L+P T G+T GK ++RI + G+ R Sbjct: 240 NKGNSFFVSNMQSYTNMYELIFITCSILIYFILIPYFTNGKTIGKALLRIHLKGKADRIT 299 Query: 283 LWGLIVRYVLQYGVLIGANVFXXXXXXXXXXXXXWTGIPYSLVMLLDFMFLIHLIIRVFK 342 L L +RY + Y L G N I + +++ +F+IH+++ VF Sbjct: 300 LKELFIRYGIFYFALGGINYILSSSSMLNHTEPLVLLITLLFLFIINGLFIIHVLLHVFS 359 Query: 343 R 343 R Sbjct: 360 R 360 >ref|NP_977073.1| membrane protein, putative [Bacillus cereus ATCC 10987] gb|AAS39681.1| membrane protein, putative [Bacillus cereus ATCC 10987] Length = 383 Score = 203 bits (517), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 128/366 (34%), Positives = 202/366 (55%), Gaps = 34/366 (9%) Query: 4 SYLFPISNAFMSFPVAALVFTLPFLVVQYRRHGYINKVRAVVXXXXXXXXXNAFYLIMLP 63 +Y+FPI AF+ FP+ A+ +PFL+ YR++GY+NK R+ + NA++L++LP Sbjct: 3 AYIFPIKTAFILFPILAMFLLIPFLIFNYRKYGYLNKWRSFILYSLLLYLLNAYFLVILP 62 Query: 64 MPASRHNAAPVGSIIQHV---PLQFIQEIRRNSNIDPGNFLSYFSIFRQPDFLLAAFNVV 120 +P + + + QH+ P FIQEI +++ +YF + ++ FL AFNV+ Sbjct: 63 LPQTYDTCSLQPANTQHMQLSPFYFIQEISNHTSAILTKPATYFYLLKESAFLQVAFNVL 122 Query: 121 LTVPFGLFLGYYFRTRWVMCILLSFGLSLLFEVTQITGIYGFFDHPYRIFDVDDLITNTL 180 LTVPFG++L YYFR ++ I +SF LSL FE+TQ+TG+YG ++ YR+FD+DDL NTL Sbjct: 123 LTVPFGIYLRYYFRRSFLQTICISFCLSLFFELTQVTGLYGIYNCAYRLFDIDDLFLNTL 182 Query: 181 GGVLGYRIALWISALLPRIEQLDSKVDYSAKRVTYTRRGIAWMLDLPIWMIPFLTLHSWG 240 GGV+G+ IA + LP+ +LDS ++ K V + RR IA +D I + + G Sbjct: 183 GGVIGFIIAPIFTYFLPKANELDSHINLETKPVGFVRRLIAMQIDWIFLSIVVPVIKNKG 242 Query: 241 MQGAYWIT-----TAVYFVLLPCC------------TGGRTFGKWVVRISISGQDGRSRL 283 +++++ T +Y ++ C T G+T GK ++RI + G+ R L Sbjct: 243 --NSFFVSNIQSYTNIYELIFITCSIFIYFIIIPYFTNGKTIGKALLRIYVKGKSDRITL 300 Query: 284 WGLIVRYVLQYGVLIGANVFXXXXXXXXXXXXXWTGIPYSLVMLLDF------MFLIHLI 337 L +RY + Y VL G N P LV++L F +F+IH++ Sbjct: 301 KELFIRYGIFYFVLGGINYILSSSSILNLKE------PLVLVVILLFQLVINGLFIIHVL 354 Query: 338 IRVFKR 343 + VF R Sbjct: 355 LHVFSR 360 >ref|NP_782003.1| putative permease [Clostridium tetani E88] gb|AAO35940.1| putative permease [Clostridium tetani E88] Length = 378 Score = 172 bits (436), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 103/294 (35%), Positives = 165/294 (56%), Gaps = 16/294 (5%) Query: 4 SYLFPISNAFMSFPVAALVFTLPFLVVQYRRHGYINKVRAVVXXXXXXXXXNAFYLIMLP 63 +Y+FPI A ++ + +P+ ++QYR+HG+++K R+ + +AFYL++LP Sbjct: 3 AYVFPIKIALLTVIFLIYLAFIPYCIIQYRKHGFVSKYRSFMHIAFVFYIISAFYLVILP 62 Query: 64 MP----ASRHNAAPVGSIIQHVPLQFIQEI-RRNSNIDPGNFLSYFSIFRQPDFLLAAFN 118 +P +H P Q +P FI + + SN+ F +Y SI + FN Sbjct: 63 LPDRNFVPKHIIHP-----QLIPFNFIGDFFKEYSNLVARGFSTYSSILKNRGLWQVFFN 117 Query: 119 VVLTVPFGLFLGYYFRTRWVMCILLSFGLSLLFEVTQITGIYGFFDHPYRIFDVDDLITN 178 V+L P G FL Y++ + + +SF SL FEVTQ+TGI+G + + YR+FDVDDL+ N Sbjct: 118 VLLLTPLGFFLRYFYNINFKKALAISFLTSLFFEVTQLTGIFGIYKYAYRLFDVDDLMLN 177 Query: 179 TLGGVLGYRIALWISALLPRIEQLDSKVDYSAKRVTYTRRGIAWMLD-LPIWMIPFLTLH 237 T+GGV+GY + +LP I+++D + +Y +V Y RRGIA+ D L I IP + Sbjct: 178 TIGGVIGYYLVPISKKILPNIDEIDLRYNYDESKVGYIRRGIAFAADLLVISFIPLINRK 237 Query: 238 SWGMQGAYWITTAVYFVLLPCCTGGRTFGKWVVRISISGQ-DGRSRLWGLIVRY 290 + + ++I +Y +LLP T G T GK +V+I I + + + + LI RY Sbjct: 238 N-DITSIWFI---LYIILLPYITNGYTLGKKLVKIKIVNESNTKLKFIELIKRY 287 >ref|ZP_00036729.1| COG4767: Glycopeptide antibiotics resistance protein [Enterococcus faecium] Length = 387 Score = 167 bits (423), Expect = 3e-40, Method: Compositional matrix adjust. Identities = 104/304 (34%), Positives = 163/304 (53%), Gaps = 22/304 (7%) Query: 4 SYLFPISNAFMSFPVAALVFTLPFLVVQYRRHGYINKVRAVVXXXXXXXXXNAFYLIMLP 63 SY+ PI A + FP AL + F + +YR++G RAV+ A++L++LP Sbjct: 3 SYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVILYSFVFYLLCAYFLVILP 62 Query: 64 MPASRHNAAPVGSIIQHVPLQFIQEIRRNSNIDPGNFLSYFSIFRQPDFLLAAFNVVLTV 123 +PA A G ++ Q R N+ + +Y +Q FL AFNV+L + Sbjct: 63 LPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTYLPAMKQSVFLEPAFNVLLLI 122 Query: 124 PFGLFLGYYFRTRWVMCILLSFGLSLLFEVTQITGIYGFFDHPYRIFDVDDLITNTLGGV 183 PFG++L YYFR ++ ++ SF LSL FE+TQ++G+Y + PYR+FDV+DL+TNTLGG+ Sbjct: 123 PFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYFIYPRPYRLFDVNDLVTNTLGGI 182 Query: 184 LGYRIALWISALLPRIEQLDSKVDYSAKRVTYTRRGIAWMLDLPIWMIPFLTL------- 236 +G+ + + +LP ++D K V+ TRR +A+++D W FL+L Sbjct: 183 IGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRLVAYLID---W--TFLSLINLVAAI 237 Query: 237 ------HSWGMQGAYW---ITTAVYFVLLPCCTGGRTFGKWVVRISISGQD-GRSRLWGL 286 S + W + VYF+++P T G T GK +VRI + +D R L L Sbjct: 238 FFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLGKKIVRIRLVEEDRERISLKAL 297 Query: 287 IVRY 290 ++RY Sbjct: 298 MIRY 301 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.333 0.147 0.481 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 482,955,302 Number of extensions: 19095189 Number of successful extensions: 71675 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 71677 Number of HSP's successfully gapped: 11 Length of query: 349 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 223 Effective length of database: 415,135,104 Effective search space: 92575128192 Effective search space used: 92575128192 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.5 bits) S2: 126 (53.1 bits)