BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0217.0002.p (447 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061479.1| COG0642: Signal transduction histidine kinase... 137 5e-31 ref|NP_691506.1| two-component sensor histidine kinase [Oceanoba... 124 5e-27 ref|NP_780903.1| sensory transduction histidine kinase [Clostrid... 102 2e-20 ref|NP_563403.1| two-component sensor histidine kinase [Clostrid... 98 3e-19 ref|NP_563155.1| two-component sensor histidine kinase [Clostrid... 94 5e-18 >ref|ZP_00061479.1| COG0642: Signal transduction histidine kinase [Clostridium thermocellum ATCC 27405] Length = 495 Score = 137 bits (345), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 121/484 (25%), Positives = 211/484 (43%), Gaps = 55/484 (11%) Query: 3 TIRGKMLLSFFLALVVTVGITVALFVRLIDDILVNQVKTQLHGQAEKAEKIL-------- 54 TI+ ++ +A+ V + +T +F I + N +K QL A++ E Sbjct: 11 TIKWRIFKYNIIAISVLLALTTIVFNITIQSYIKNDIKEQLSTIAQRVEDTALYRGPDFF 70 Query: 55 -------------QDSDISRLNNTEFKYVV--------KGMMLNADYLILDANNKIIDAN 93 + + + R NN F++ +LNAD+++LD N II Sbjct: 71 PPQEPQRPRPLPGRQNGMFRENNDLFRFYFFLDRSLREPLSILNADFILLDNNMDIITPP 130 Query: 94 EDQGVGTTLHYALSERQGIAVLHGKKIL-YTKERLSGRPYSIFIYAPLSSLRA------- 145 E + A I L G K+ Y ++ Y I I P+S + Sbjct: 131 EGDFFKPSGEIANEIISKIKKLDGSKVQEYVYFNVNNTDY-ISIVKPVSDKNSFGLGWLI 189 Query: 146 LYGSLMRTTLLAIGFSFFMILAIGL----------LTVSRVVRPLNRLKEAVSRY-EPYR 194 +Y SL + L +G + +I+ + L L ++ P + L + + E Sbjct: 190 IYSSLQKVNQLKLGINMILIVIVLLSALIFAIFSSLAAKKISAPFSSLNQHIRAIAERNF 249 Query: 195 SQDAVFPVDDTTEIGELITTFQSMSERIQQHQRNQVEFLQNVSHELKTPLMSIHGFVFAI 254 PVD E+ EL+ + MSE+++ + + Q F QN SHE +TPLMSI + I Sbjct: 250 GVKIEMPVD--AELQELVNSINIMSEKLESYDKAQKTFFQNASHEFRTPLMSIQSYAEGI 307 Query: 255 QDQVVTQETGLDVIMNQSLRLIDMVDKLLQLSRLEAVDEHWPISEIDLRTXXXXXXXXXX 314 + VV + T D+I++++ R+ +V+ LL LSRL+ ++E++ +++D + Sbjct: 308 KYDVVDKNTAADIIIDETKRMTHLVEDLLYLSRLDTIEENYRFADLDFKDFINACQERMY 367 Query: 315 XXXXXRGVALKVE--GESLLTVAAGEXXXXXXXXXXXXAVRHTSSQIRVQIEAGYPGAGW 372 G+ L+VE E ++ A E +R+ ++ + + + + Sbjct: 368 GIASKSGINLEVENINEPVVVYADEEKLFRAFANIIGNCIRYANNTVSI-VPKIIDRSKI 426 Query: 373 AIHIDDDGNGLSEAEREAVFQRFYTGPNGVTGLGLAICRQIAANLDGELTYSSSPLGGAR 432 I I DDG G+ E +F+RFY G G GLGLAI + I +G++T +S GA Sbjct: 427 EIVIRDDGPGIDSNELPNIFERFYKGKKGNVGLGLAISKNIIERHNGKITARNSE-SGAV 485 Query: 433 FSFI 436 F+ + Sbjct: 486 FTIV 489 >ref|NP_691506.1| two-component sensor histidine kinase [Oceanobacillus iheyensis HTE831] dbj|BAC12541.1| two-component sensor histidine kinase [Oceanobacillus iheyensis HTE831] Length = 460 Score = 124 bits (311), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 83/320 (25%), Positives = 158/320 (49%), Gaps = 10/320 (3%) Query: 119 KILYTKERLSGRPYSIFIYAPLSSLRALYGSLMRTTLLAIGFSFFMILAIGLLTVSRVVR 178 +I+++ E+ + + P+ L+ + + LL + + +++V Sbjct: 126 RIIFSPEQTG---LELILLTPMHDLQVVQQNFFVRLLLVFLVGAVAAVILSYFMTNKLVT 182 Query: 179 PLNRLKEAVSRYEPYRSQDAVFPVDDTTEIGELITTFQSMSERIQQHQRNQVEFLQNVSH 238 PL++LK + + E R D + V T EI E+ + M++ ++Q+ ++Q F QN SH Sbjct: 183 PLSQLKTELKKVEN-RQFDRMSRVKATGEIKEVEQSVYEMAQTLEQYMKSQQTFFQNASH 241 Query: 239 ELKTPLMSIHGFVFAIQDQVVTQ---ETGLDVIMNQSLRLIDMVDKLLQLSRLEAVDEHW 295 ELKTPLM+I G+ I+D + + E GL+V++ + RL ++++++ L++L+ + Sbjct: 242 ELKTPLMTIQGYAEGIRDGIFDEKDTEKGLEVMVTEVNRLKGIINEMILLAKLDTDPAEY 301 Query: 296 PISEIDLRTXXXXXXXXXXXXXXXRGVALKVEGES-LLTVAAGEXXXXXXXXXXXXAVRH 354 ++ G+ L + E+ ++ A E +RH Sbjct: 302 SPFPLEADQFIHQVVDRAHPIVNEEGIELNHQVENGVILFADEEKLLRALLNIVFNGIRH 361 Query: 355 TSSQIRVQIEAGYPGAGWAIHIDDDGNGLSEAEREAVFQRFYTGPNGVTGLGLAICRQIA 414 + S + +Q+ I ++DDG+G++E + E +F RF G +G TGLGLAI R I Sbjct: 362 SKSTVNIQVYT--KDHKVMIDVEDDGSGITEEDAEHIFHRFVKGKDGETGLGLAIARAII 419 Query: 415 ANLDGELTYSSSPLGGARFS 434 G ++ S+S +GGA+F+ Sbjct: 420 EQAGGNISVSNSEIGGAKFT 439 >ref|NP_780903.1| sensory transduction histidine kinase [Clostridium tetani E88] gb|AAO34840.1| sensory transduction histidine kinase [Clostridium tetani E88] Length = 468 Score = 102 bits (255), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 78/303 (25%), Positives = 141/303 (46%), Gaps = 15/303 (4%) Query: 143 LRALYGSLMRTTLLAIGFSFFMILAIGLLTVSRVVRPLNRLKEAVSRYEPYRSQDAVFPV 202 L+ +Y + + +LAI FSFF+I + + + +N + + +S+ E D V Sbjct: 166 LKKVYNIIWLSAILAIMFSFFVIYYFSQRIIIKPLEKINYVADKISKGEV----DKRVFV 221 Query: 203 DDTTEIGELITTFQSMSERIQQHQRNQVEFLQNVSHELKTPLMSIHGFVFAIQDQVVTQE 262 + EIG+L +F +M+E +++ + N+ F+ NVSHE+++P+ SI GF+ I D V+ E Sbjct: 222 NSEDEIGDLANSFNAMAESLEKIEENRRSFISNVSHEIRSPITSIKGFISGILDGVIPSE 281 Query: 263 TG---LDVIMNQSLRLIDMVDKLLQLSRLEAVDEHWPISEIDLRTXXXXXXXXXXXXXXX 319 L + +++ RL +V+ LL LS +EA I E+++ Sbjct: 282 KQNYYLALAYDETQRLTRLVNDLLDLSAIEAGQLKLRIEEVNINEIIRLTVIKFKPLIDR 341 Query: 320 RG--VALKVEGESLLTVAAGEXXXXXXXXXXXXAVRHTSSQIRVQIEAGYPGAGWAIHID 377 + V + ++ + L V + A+++T +V++ G + I Sbjct: 342 KNLDVDICLQEDDLYVVGDKDRLFQVMINLIDNAIKYTVENGKVEVRTKLKGNKVYVSIY 401 Query: 378 DDGNGLSEAEREAVFQRFYTGPNG-----VTGLGLAICRQIAANLDGELTYSSSPLGGAR 432 + G + E + + V+ RFY TGLGL+I R I L GE + + G Sbjct: 402 NSGKHIPEEDIKNVWSRFYKVDKSRTSKVSTGLGLSIVRSILTQL-GEDIWVKNVDAGVL 460 Query: 433 FSF 435 F+F Sbjct: 461 FTF 463 >ref|NP_563403.1| two-component sensor histidine kinase [Clostridium perfringens] dbj|BAB82193.1| two-component sensor histidine kinase [Clostridium perfringens str. 13] Length = 469 Score = 98.2 bits (243), Expect = 3e-19, Method: Compositional matrix adjust. Identities = 116/475 (24%), Positives = 209/475 (44%), Gaps = 56/475 (11%) Query: 2 RTIRGKMLLSFFLALVVT---VGITVALFVRLIDDILVNQVKTQLHGQAEKAEKILQDSD 58 R + K++L+ + + +T VGI ++ R ++ ++KT L G+AE E+++ Sbjct: 4 RGLVPKLILTLSVTICITFFIVGIILSSLFR--EEYYQERLKT-LSGEAEVIEQVVNSFM 60 Query: 59 ISRLNNTEFKYVVK--GMMLNADYLILDANNKI---------------IDANEDQGVGTT 101 S E + G L AD ++D+ I ID N+ + + Sbjct: 61 NSETTLGELDKSISFIGDSLKADIYLIDSYGYIYSVSNEKYDYLKRTKIDDNDMKTLKEN 120 Query: 102 LHYALSERQGIAVLHGKKILYTKERLSGRPYSIFIYAPLSSLRALYGSLMRTT-----LL 156 + + +++ ++ K I Y K +G ++ I PLS AL SL+R + Sbjct: 121 I--PIEQKKKNEFIYSKPIFY-KGVFTG---AVSIVTPLS---ALSDSLVRINQIVWMCV 171 Query: 157 AIGFSFFMILAIGLLTVSRVVRPLNRLKEAVSRYEPYRSQDAV-FPVDDTTEIGELITTF 215 AI F LAI ++ +++PL + A + + V DD E+GEL +F Sbjct: 172 AIAI-IFSGLAIYFISKKIIIKPLKVINNAAKKIANGEIGNRVNVSYDD--EVGELAESF 228 Query: 216 QSMSERIQQHQRNQVEFLQNVSHELKTPLMSIHGFVFAIQDQVV---TQETGLDVIMNQS 272 M+ I++ + N+ EF+ NVSHEL++P+ SI GF+ AI D V+ +E L ++ ++ Sbjct: 229 NIMATSIEEAELNRREFISNVSHELRSPMTSIKGFITAILDGVIPKDKEEYYLVIVNDEI 288 Query: 273 LRLIDMVDKLLQLSRLEAVDEHWPISEIDLRTXXXXXXXXXXXXXXXRGVALKV--EGES 330 RL +++ LL LS ++A + ISE++L + + ++V E E Sbjct: 289 SRLTRLINDLLDLSAMQAGKLEFNISELELNRIIETTVLKMNQKAASKNIKIEVLLESEK 348 Query: 331 LLTVAAGEXXXXXXXXXXXXAVRHTSSQIRVQIEAGYPGAGWAIHIDDDGNGLSEAEREA 390 L + A+++ V+I G+ + I + G+ E + Sbjct: 349 LYVYGDNDRLIQVVTNLVDNAIKYCDENGEVKISTKTKGSKIIVSIFNTSKGIDEYDLTH 408 Query: 391 VFQRFYT-----GPNGVTGLGLAICRQIAANLDGEL-----TYSSSPLGGARFSF 435 ++ FY TGLGL+I R I L+ ++ + + G RF+F Sbjct: 409 IWDIFYKVDKARSNKTSTGLGLSIVRNIILQLEEDIWAENVKDKNGEIKGVRFNF 463 >ref|NP_563155.1| two-component sensor histidine kinase [Clostridium perfringens] dbj|BAB81945.1| two-component sensor histidine kinase [Clostridium perfringens str. 13] Length = 471 Score = 94.4 bits (233), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 111/464 (23%), Positives = 205/464 (44%), Gaps = 43/464 (9%) Query: 1 MRTIRGKMLLSFFLALVVTVGITVALFVRLI--------DDILVNQVKTQLH------GQ 46 M++I+ +++ +F + L+ T+ I A+F+ I ++IL++Q+ + Sbjct: 1 MKSIKTRLMKNFLVLLLSTIIIVSAMFLIFISRYYYQNTEEILLSQINISVDFYKRYLSN 60 Query: 47 AEKAEKILQDSDI-SRLNNTEFK-YVVKGMMLNADYLILDANNKIIDANEDQGVGTTLHY 104 E + +D DI + + + + Y +KG ++ D + L+ + N V L Sbjct: 61 VSLEENVYEDVDIFWKQTDAQVQIYNLKGQLI-MDSIGLEPK----EYNTPVDVKRALEG 115 Query: 105 ALSERQGIAVLHGKKILYTKERLSGRPYSIF-IYAPLSSLRALYGSLMRTTL--LAIGFS 161 ++ G + K++ L I + +SSLR + ++ L L IG + Sbjct: 116 DTAKWVGTVPDYTGKVMAISAPLRNSSNEIVGVIRYISSLRNVDNFILNFFLVFLVIGLT 175 Query: 162 FFMILAIGLLT----VSRVVRPLNRLKEAVSRYEP--YRSQDAVFPVDDTTEIGELITTF 215 +LAIG++ + +V P+ L + + + ++ + D EI +L + Sbjct: 176 ---VLAIGIILSYFLANSIVNPITELIKVSEQMAKGNLKVRNKIVTND---EIEKLADSL 229 Query: 216 QSMSERIQQHQRNQVEFLQNVSHELKTPLMSIHGFVFAIQDQVVTQET---GLDVIMNQS 272 M+E I+ + + EF+ +VSHEL+TPL SI G+ + + +ET G D+I ++ Sbjct: 230 NIMAEEIENREILKNEFISSVSHELRTPLTSIKGWAITLNNDFTDRETLKMGFDIIEKEA 289 Query: 273 LRLIDMVDKLLQLSRLEAVDEHWPISEIDLRTXXXXXXXXXXXXXXXRGVALKVEG--ES 330 RL +MV++LL S+ + EI+L+ L ++G E Sbjct: 290 DRLSNMVEELLDFSKFVSGKIKLKYEEINLKEFIEYLRLYMNPRAEREHKELILKGVTED 349 Query: 331 LLTVAAGEXXXXXXXXXXXXAVRHTSSQIRVQIEAGYPGAGWAIHIDDDGNGLSEAEREA 390 + V + A + T ++ IE Y G I+I D G G+S+ E Sbjct: 350 FIIVGDKDRLKQVFINIIDNAFKFTHENEKITIEFVYADEGIYINIIDTGCGISKEELPR 409 Query: 391 VFQRFYTGPNGVT--GLGLAICRQIAANLDGELTYSSSPLGGAR 432 V ++FY G N + G+GL+IC +I A +G L S G + Sbjct: 410 VKEKFYKGKNSKSQNGIGLSICDEIIALHEGTLEIYSELGNGTK 453 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.137 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 554,649,409 Number of extensions: 21136282 Number of successful extensions: 70999 Number of sequences better than 1.0e-05: 898 Number of HSP's gapped: 71990 Number of HSP's successfully gapped: 900 Length of query: 447 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 318 Effective length of database: 406,989,807 Effective search space: 129422758626 Effective search space used: 129422758626 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 127 (53.5 bits)