BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0216.0002.p (199 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAA02944.1| unnamed protein product [Bacillus subtilis] >gi|... 225 5e-58 dbj|BAA02945.1| unnamed protein product [Bacillus subtilis] 225 5e-58 dbj|BAA02946.1| unnamed protein product [Bacillus subtilis] 225 6e-58 ref|NP_388196.1| ATP-binding membrane protein [Bacillus subtilis... 223 2e-57 ref|NP_825859.1| putative TmrB-like protein [Streptomyces avermi... 144 1e-33 >dbj|BAA02944.1| unnamed protein product [Bacillus subtilis] prf||1410166A tunicamycin resistance gene tmrB Length = 206 Score = 225 bits (573), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 103/196 (52%), Positives = 137/196 (69%) Query: 1 MILWINGAFGSGKTQTAFELNRRIPHSFVFDPENAGYYIQKNIPSTLKKNDFQNYSQWRE 60 MI+WINGAFGSGKTQTAFEL+RR+ S+V+DPE G+ ++ +P + K+DFQ+Y WR Sbjct: 10 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 69 Query: 61 FNYSMLRYIASEFEGIIIVPMTIVEPDYFREIVGRLREDGIKVYHYTLYVXXXXXXXXXX 120 FNYS+L + + GI+IVPMTIV P+YF EI+GRLR++G V+H+TL Sbjct: 70 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 129 Query: 121 XXGEGKHSWAANQIDRCLKGHQHPIFAEQLVTDNMSIEQVAEAIAAKLQISLLPAAPGRM 180 EGK+SWAA QIDRC++G PIF + + TDN+SI+ VAE IAA+ ++ L P G + Sbjct: 130 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 189 Query: 181 RRFIQRLKVKYQHIRL 196 RRF RL VK HIR+ Sbjct: 190 RRFADRLMVKLNHIRI 205 >dbj|BAA02945.1| unnamed protein product [Bacillus subtilis] Length = 199 Score = 225 bits (573), Expect = 5e-58, Method: Compositional matrix adjust. Identities = 103/196 (52%), Positives = 137/196 (69%) Query: 1 MILWINGAFGSGKTQTAFELNRRIPHSFVFDPENAGYYIQKNIPSTLKKNDFQNYSQWRE 60 MI+WINGAFGSGKTQTAFEL+RR+ S+V+DPE G+ ++ +P + K+DFQ+Y WR Sbjct: 3 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 62 Query: 61 FNYSMLRYIASEFEGIIIVPMTIVEPDYFREIVGRLREDGIKVYHYTLYVXXXXXXXXXX 120 FNYS+L + + GI+IVPMTIV P+YF EI+GRLR++G V+H+TL Sbjct: 63 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 122 Query: 121 XXGEGKHSWAANQIDRCLKGHQHPIFAEQLVTDNMSIEQVAEAIAAKLQISLLPAAPGRM 180 EGK+SWAA QIDRC++G PIF + + TDN+SI+ VAE IAA+ ++ L P G + Sbjct: 123 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 182 Query: 181 RRFIQRLKVKYQHIRL 196 RRF RL VK HIR+ Sbjct: 183 RRFADRLMVKLNHIRI 198 >dbj|BAA02946.1| unnamed protein product [Bacillus subtilis] Length = 197 Score = 225 bits (573), Expect = 6e-58, Method: Compositional matrix adjust. Identities = 103/196 (52%), Positives = 137/196 (69%) Query: 1 MILWINGAFGSGKTQTAFELNRRIPHSFVFDPENAGYYIQKNIPSTLKKNDFQNYSQWRE 60 MI+WINGAFGSGKTQTAFEL+RR+ S+V+DPE G+ ++ +P + K+DFQ+Y WR Sbjct: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPEKMGFALRSMVPQEIAKDDFQSYPLWRA 60 Query: 61 FNYSMLRYIASEFEGIIIVPMTIVEPDYFREIVGRLREDGIKVYHYTLYVXXXXXXXXXX 120 FNYS+L + + GI+IVPMTIV P+YF EI+GRLR++G V+H+TL Sbjct: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120 Query: 121 XXGEGKHSWAANQIDRCLKGHQHPIFAEQLVTDNMSIEQVAEAIAAKLQISLLPAAPGRM 180 EGK+SWAA QIDRC++G PIF + + TDN+SI+ VAE IAA+ ++ L P G + Sbjct: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180 Query: 181 RRFIQRLKVKYQHIRL 196 RRF RL VK HIR+ Sbjct: 181 RRFADRLMVKLNHIRI 196 >ref|NP_388196.1| ATP-binding membrane protein [Bacillus subtilis] sp|P12921|TMRB_BACSU Tunicamycin resistance protein pir||YTBST tunicamycin resistance protein - Bacillus subtilis dbj|BAA08948.1| tunicamycin resistance protein [Bacillus subtilis] emb|CAB12108.1| ATP-binding membrane protein [Bacillus subtilis subsp. subtilis str. 168] Length = 197 Score = 223 bits (569), Expect = 2e-57, Method: Compositional matrix adjust. Identities = 102/196 (52%), Positives = 137/196 (69%) Query: 1 MILWINGAFGSGKTQTAFELNRRIPHSFVFDPENAGYYIQKNIPSTLKKNDFQNYSQWRE 60 MI+WINGAFGSGKTQTAFEL+RR+ S+V+DP+ G+ ++ +P + K+DFQ+Y WR Sbjct: 1 MIIWINGAFGSGKTQTAFELHRRLNPSYVYDPQKMGFALRSMVPQEIAKDDFQSYPLWRA 60 Query: 61 FNYSMLRYIASEFEGIIIVPMTIVEPDYFREIVGRLREDGIKVYHYTLYVXXXXXXXXXX 120 FNYS+L + + GI+IVPMTIV P+YF EI+GRLR++G V+H+TL Sbjct: 61 FNYSLLASLTDTYRGILIVPMTIVHPEYFNEIIGRLRQEGRIVHHFTLMASKETLLKRLR 120 Query: 121 XXGEGKHSWAANQIDRCLKGHQHPIFAEQLVTDNMSIEQVAEAIAAKLQISLLPAAPGRM 180 EGK+SWAA QIDRC++G PIF + + TDN+SI+ VAE IAA+ ++ L P G + Sbjct: 121 TRAEGKNSWAAKQIDRCVEGLSSPIFEDHIQTDNLSIQDVAENIAARAELPLDPDTRGSL 180 Query: 181 RRFIQRLKVKYQHIRL 196 RRF RL VK HIR+ Sbjct: 181 RRFADRLMVKLNHIRI 196 >ref|NP_825859.1| putative TmrB-like protein [Streptomyces avermitilis MA-4680] dbj|BAC72394.1| putative TmrB-like protein [Streptomyces avermitilis MA-4680] Length = 210 Score = 144 bits (362), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 73/209 (34%), Positives = 112/209 (53%), Gaps = 15/209 (7%) Query: 1 MILWINGAFGSGKTQTAFELNRRIPHSFVFDPENAGYYIQKNIPSTLKKNDFQNYSQWRE 60 M+LWING FG GKTQTA E+ RR+P S + DPE+ G+ +++ +P L+ N FQ+ + WR+ Sbjct: 1 MLLWINGPFGGGKTQTAHEIQRRLPGSVICDPEHVGFGLRRMLPPGLRAN-FQDLASWRQ 59 Query: 61 FNYSMLRYIASEFEGIIIVPMTIVEPDYFREIVGRLREDGIKVYHYTLYVXXXXXXXXXX 120 +L + +G++I PMTI + F + VGRLRE G V H+ L Sbjct: 60 GVVEVLDLALTRHDGVVIAPMTITDSGCFAQTVGRLRELGHDVRHFALLAERETVLRRLR 119 Query: 121 XXGEG--------------KHSWAANQIDRCLKGHQHPIFAEQLVTDNMSIEQVAEAIAA 166 G G SWA Q+D CL+ + P F E L TD ++ + A+ IA Sbjct: 120 ERGFGHLLRYVAGKDAPLRGESWAVGQLDHCLERLREPEFGEHLWTDQSTVPKTADRIAV 179 Query: 167 KLQISLLPAAPGRMRRFIQRLKVKYQHIR 195 ++L P G +R +++++V +H+R Sbjct: 180 LAGLTLAPNTEGALRTRLRQVRVGVKHMR 208 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.324 0.139 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 281,258,222 Number of extensions: 10303820 Number of successful extensions: 33990 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 33975 Number of HSP's successfully gapped: 19 Length of query: 199 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 80 Effective length of database: 434,140,797 Effective search space: 34731263760 Effective search space used: 34731263760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 123 (52.0 bits)