BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0212.0004.p (315 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060122.1| hypothetical protein [Clostridium thermocellu... 86 1e-15 ref|ZP_00060580.1| hypothetical protein [Clostridium thermocellu... 82 1e-14 ref|ZP_00060931.1| COG2340: Uncharacterized protein with SCP/PR1... 79 2e-13 ref|ZP_00060970.1| COG4880: Secreted protein containing C-termin... 74 4e-12 ref|ZP_00062184.1| hypothetical protein [Clostridium thermocellu... 74 4e-12 >ref|ZP_00060122.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 265 Score = 86.3 bits (212), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 41/118 (34%), Positives = 67/118 (56%) Query: 49 NGIYANMDVAPYITKGTTMVPLQVAQQIPGSSVQWNNASKTVTLTRNGETITLVAGQKTA 108 NG+ DVAPYI G TMVP + + G + WN ++T+ T + I + G+ A Sbjct: 51 NGVEIKFDVAPYIKNGRTMVPFRAIFEALGVDISWNGVNRTILATNDTTEIYIEIGKAFA 110 Query: 109 KVGNKEVKLEAASTLKQGRVMVPLRFIAESTGAYVLWNPKQRIVFVAKSSEEEELKEQ 166 V +V L+A + + GR VPLRF++E+ GA V W+ +R V+++ ++ +L E+ Sbjct: 111 YVNGYKVNLDAEAEIVGGRTFVPLRFVSENAGADVSWDGARRTVYISYVNQVRDLGEK 168 >ref|ZP_00060580.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 591 Score = 82.4 bits (202), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 40/98 (40%), Positives = 59/98 (60%), Gaps = 1/98 (1%) Query: 56 DVAPYITKGT-TMVPLQVAQQIPGSSVQWNNASKTVTLTRNGETITLVAGQKTAKVGNKE 114 D P+I + TMVP++ + + V W + V + + G I+LV G KTAKV KE Sbjct: 40 DQQPFIDSNSRTMVPIRFISEALDAKVDWIEKERMVVIKKQGTEISLVVGMKTAKVNGKE 99 Query: 115 VKLEAASTLKQGRVMVPLRFIAESTGAYVLWNPKQRIV 152 +KL+ +S + GR VP+RFI+E+ GA V W+ K +IV Sbjct: 100 IKLDTSSVIAGGRTFVPVRFISEAFGATVEWDGKNKIV 137 >ref|ZP_00060931.1| COG2340: Uncharacterized protein with SCP/PR1 domains [Clostridium thermocellum ATCC 27405] Length = 451 Score = 78.6 bits (192), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 40/114 (35%), Positives = 61/114 (53%) Query: 49 NGIYANMDVAPYITKGTTMVPLQVAQQIPGSSVQWNNASKTVTLTRNGETITLVAGQKTA 108 NG Y +DV P I G T+VP++ + + V+W SK V + +N ITL A Sbjct: 39 NGQYLQLDVPPVIENGRTLVPVRGVFESINADVEWVPESKKVNVYKNDIVITLKINSNIA 98 Query: 109 KVGNKEVKLEAASTLKQGRVMVPLRFIAESTGAYVLWNPKQRIVFVAKSSEEEE 162 + + V+L+ + + +GR +VP+RFI+ES GA V W+ R V + E E Sbjct: 99 YINDNAVRLDVPARIVEGRTLVPVRFISESIGASVGWDDATRTVIINTYDENVE 152 >ref|ZP_00060970.1| COG4880: Secreted protein containing C-terminal beta-propeller domain distantly related to WD-40 repeats [Clostridium thermocellum ATCC 27405] Length = 845 Score = 74.3 bits (181), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 34/93 (36%), Positives = 54/93 (58%) Query: 54 NMDVAPYITKGTTMVPLQVAQQIPGSSVQWNNASKTVTLTRNGETITLVAGQKTAKVGNK 113 N +V PYI G T+VP++ + G+ V W+ + VT+ N + + LV G K KVG+K Sbjct: 81 NANVKPYIKDGRTLVPVRFVSESIGAQVDWDRKNSRVTVKLNNKVVELVIGNKIMKVGDK 140 Query: 114 EVKLEAASTLKQGRVMVPLRFIAESTGAYVLWN 146 E L+ A + +GR +PLR + E+ G V ++ Sbjct: 141 EEVLDVAPEISEGRTYLPLRSLVEALGKKVFYD 173 >ref|ZP_00062184.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 243 Score = 73.9 bits (180), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 34/99 (34%), Positives = 59/99 (59%) Query: 56 DVAPYITKGTTMVPLQVAQQIPGSSVQWNNASKTVTLTRNGETITLVAGQKTAKVGNKEV 115 DV P G +VP++ + G+ V +N+A+K V + ++ I L G + A V +++ Sbjct: 143 DVKPENVNGRVLVPIRTISEEMGAEVTYNDATKQVLIAKDDTEILLTIGSQEAYVNGEKI 202 Query: 116 KLEAASTLKQGRVMVPLRFIAESTGAYVLWNPKQRIVFV 154 L+ + + +GR MVPLRFI+ES A V W+ + +IV++ Sbjct: 203 MLDVPAMIIEGRTMVPLRFISESMNATVEWDGEAQIVYI 241 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.132 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 419,778,845 Number of extensions: 15357800 Number of successful extensions: 27588 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 27570 Number of HSP's successfully gapped: 26 Length of query: 315 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 190 Effective length of database: 417,850,203 Effective search space: 79391538570 Effective search space used: 79391538570 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 126 (53.1 bits)