BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0210.0006.p (339 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAA31551.1| XynX [Aeromonas punctata] 484 e-135 pir||JC7139 endo-1,4-beta-xylanase (EC 3.2.1.8) - Aeromonas caviae 481 e-134 emb|CAA07174.1| endo-1,4-beta-xylanase [Bacillus sp. BP-23] 478 e-134 pdb|1N82|A Chain A, The High-Resolution Crystal Structure Of Ixt... 411 e-113 pir||I40356 endo-beta-1,4-xylanase - Bacillus sp >gi|662884|emb|... 411 e-113 >dbj|BAA31551.1| XynX [Aeromonas punctata] Length = 333 Score = 484 bits (1246), Expect = e-135, Method: Compositional matrix adjust. Identities = 221/326 (67%), Positives = 271/326 (83%) Query: 6 SEIPALHEMFREAFKIGAAVSTGIIAAQGPFIARHYNSITAENEMKPALVQPEEGEFTFE 65 +EIP+LH + FKIGAAV T ++ ++G FIA+H+NSITAEN+MK + PEE ++FE Sbjct: 3 TEIPSLHAAYANTFKIGAAVHTRMLQSEGEFIAKHFNSITAENQMKFEEIHPEEDRYSFE 62 Query: 66 AADGIFEFAESHGIGVRGHTLLWHNQTGDWMFREAEGGTCSREQLLARLQTHINTVVGRY 125 AAD I +FA + GIGVRGHTL+WHNQT W+F + G SRE LL+RL+ HI+TVVGRY Sbjct: 63 AADQIVDFAVAQGIGVRGHTLVWHNQTSKWVFEDTSGAPASRELLLSRLKQHIDTVVGRY 122 Query: 126 RGRGYAWDVVNEAIEDKTDQYLRDTRWLEIIGEDYLRQAFEMAHQADPDALLFYNDYNET 185 +G+ YAWDVVNEA+EDKTD ++RDT+WLE++GEDYL QAF MAH+ADP+ALLFYNDYNET Sbjct: 123 KGQIYAWDVVNEAVEDKTDLFMRDTKWLELVGEDYLLQAFSMAHEADPNALLFYNDYNET 182 Query: 186 DPVKSGKIYKLVRSLLDQNTPIHGIGMQGHWNIYGPSIEEIRNALKLYASLGLKIHVTEL 245 DPVK KIY LVRSLLD+ P+HGIG+QGHWNI+GPSIEEIR A++ YASL +++HVTEL Sbjct: 183 DPVKREKIYNLVRSLLDKGAPVHGIGLQGHWNIHGPSIEEIRMAIERYASLDVQLHVTEL 242 Query: 246 DLSVFRHDDKRTDLKAPTDEMLKLQEERYAEIFALLLEFRDSIDSVTFWGVADDYTWLDG 305 D+SVFRH+D+RTDL APT EM +LQE RY EIF L E++ SI SVTFWGVAD+YTWLD Sbjct: 243 DMSVFRHEDRRTDLTAPTSEMAELQELRYEEIFNLFREYKSSITSVTFWGVADNYTWLDH 302 Query: 306 FPVRGRKNWPFLFDEQKQPKASFARL 331 FPVRGRKNWPF+FD+Q QPK SF R+ Sbjct: 303 FPVRGRKNWPFVFDQQLQPKESFWRI 328 >pir||JC7139 endo-1,4-beta-xylanase (EC 3.2.1.8) - Aeromonas caviae Length = 333 Score = 481 bits (1238), Expect = e-134, Method: Compositional matrix adjust. Identities = 220/326 (67%), Positives = 270/326 (82%) Query: 6 SEIPALHEMFREAFKIGAAVSTGIIAAQGPFIARHYNSITAENEMKPALVQPEEGEFTFE 65 +EIP+LH + FKIGAAV T ++ ++G FIA+H+NSITAEN+MK + PEE ++FE Sbjct: 3 TEIPSLHAAYANTFKIGAAVHTRMLQSEGEFIAKHFNSITAENQMKFEEIHPEEDRYSFE 62 Query: 66 AADGIFEFAESHGIGVRGHTLLWHNQTGDWMFREAEGGTCSREQLLARLQTHINTVVGRY 125 AAD I + A + GIGVRGHTL+WHNQT W+F + G SRE LL+RL+ HI+TVVGRY Sbjct: 63 AADQIVDCAVAQGIGVRGHTLVWHNQTSKWVFEDTSGAPASRELLLSRLKQHIDTVVGRY 122 Query: 126 RGRGYAWDVVNEAIEDKTDQYLRDTRWLEIIGEDYLRQAFEMAHQADPDALLFYNDYNET 185 +G+ YAWDVVNEA+EDKTD ++RDT+WLE++GEDYL QAF MAH+ADP+ALLFYNDYNET Sbjct: 123 KGQIYAWDVVNEAVEDKTDLFMRDTKWLELVGEDYLLQAFSMAHEADPNALLFYNDYNET 182 Query: 186 DPVKSGKIYKLVRSLLDQNTPIHGIGMQGHWNIYGPSIEEIRNALKLYASLGLKIHVTEL 245 DPVK KIY LVRSLLD+ P+HGIG+QGHWNI+GPSIEEIR A++ YASL +++HVTEL Sbjct: 183 DPVKREKIYNLVRSLLDKGAPVHGIGLQGHWNIHGPSIEEIRMAIERYASLDVQLHVTEL 242 Query: 246 DLSVFRHDDKRTDLKAPTDEMLKLQEERYAEIFALLLEFRDSIDSVTFWGVADDYTWLDG 305 D+SVFRH+D+RTDL APT EM +LQE RY EIF L E++ SI SVTFWGVAD+YTWLD Sbjct: 243 DMSVFRHEDRRTDLTAPTSEMAELQELRYEEIFNLFREYKSSITSVTFWGVADNYTWLDH 302 Query: 306 FPVRGRKNWPFLFDEQKQPKASFARL 331 FPVRGRKNWPF+FD+Q QPK SF R+ Sbjct: 303 FPVRGRKNWPFVFDQQLQPKESFWRI 328 >emb|CAA07174.1| endo-1,4-beta-xylanase [Bacillus sp. BP-23] Length = 332 Score = 478 bits (1231), Expect = e-134, Method: Compositional matrix adjust. Identities = 221/327 (67%), Positives = 270/327 (82%) Query: 5 NSEIPALHEMFREAFKIGAAVSTGIIAAQGPFIARHYNSITAENEMKPALVQPEEGEFTF 64 ++EIP+L + +FKIGAAV T ++ +G FIA+HYNS+TAEN+MK V P E E+TF Sbjct: 2 STEIPSLSASYANSFKIGAAVHTRMLQTEGEFIAKHYNSVTAENQMKFEEVHPREHEYTF 61 Query: 65 EAADGIFEFAESHGIGVRGHTLLWHNQTGDWMFREAEGGTCSREQLLARLQTHINTVVGR 124 EAAD I +FA + GIGVRGHTL+WHNQT WMF +A GGT SRE +L+RL+ HI+TVVGR Sbjct: 62 EAADEIVDFAVARGIGVRGHTLVWHNQTPAWMFEDASGGTASREMMLSRLKQHIDTVVGR 121 Query: 125 YRGRGYAWDVVNEAIEDKTDQYLRDTRWLEIIGEDYLRQAFEMAHQADPDALLFYNDYNE 184 Y+ + YAWDVVNEAIEDKTD +RDT+WL ++GEDYL QAF MAH+ADP+ALLFYNDYNE Sbjct: 122 YKDQIYAWDVVNEAIEDKTDLIMRDTKWLRLLGEDYLVQAFNMAHEADPNALLFYNDYNE 181 Query: 185 TDPVKSGKIYKLVRSLLDQNTPIHGIGMQGHWNIYGPSIEEIRNALKLYASLGLKIHVTE 244 TDPVK KIY LVRSLLDQ P+HGIGMQGHWNI+GPS++EIR A++ YASL +++HVTE Sbjct: 182 TDPVKREKIYNLVRSLLDQGAPVHGIGMQGHWNIHGPSMDEIRQAIERYASLDVQLHVTE 241 Query: 245 LDLSVFRHDDKRTDLKAPTDEMLKLQEERYAEIFALLLEFRDSIDSVTFWGVADDYTWLD 304 LDLSVFRH+D+RTDL PT EM +LQ++RY +IF L E+R +I SVTFWGVAD+YTWLD Sbjct: 242 LDLSVFRHEDQRTDLTEPTAEMAELQQKRYEDIFGLFREYRSNITSVTFWGVADNYTWLD 301 Query: 305 GFPVRGRKNWPFLFDEQKQPKASFARL 331 FPVRGRKNWPF+FD + QPK SF R+ Sbjct: 302 NFPVRGRKNWPFVFDTELQPKDSFWRI 328 >pdb|1N82|A Chain A, The High-Resolution Crystal Structure Of Ixt6, A Thermophilic, Intracellular Xylanase From G. Stearothermophilus pdb|1N82|B Chain B, The High-Resolution Crystal Structure Of Ixt6, A Thermophilic, Intracellular Xylanase From G. Stearothermophilus Length = 331 Score = 411 bits (1057), Expect = e-113, Method: Compositional matrix adjust. Identities = 190/326 (58%), Positives = 248/326 (76%) Query: 5 NSEIPALHEMFREAFKIGAAVSTGIIAAQGPFIARHYNSITAENEMKPALVQPEEGEFTF 64 NS +P+L ++F F+IGAAV+ I Q + H NSITAEN MK +QPEEG+FTF Sbjct: 2 NSSLPSLRDVFANDFRIGAAVNPVTIEMQKQLLIDHVNSITAENHMKFEHLQPEEGKFTF 61 Query: 65 EAADGIFEFAESHGIGVRGHTLLWHNQTGDWMFREAEGGTCSREQLLARLQTHINTVVGR 124 + AD I +FA SH + VRGHTL+WHNQT DW+F++ +G SR+ LL R++ HI+TVV R Sbjct: 62 QEADRIVDFACSHRMAVRGHTLVWHNQTPDWVFQDGQGHFVSRDVLLERMKCHISTVVRR 121 Query: 125 YRGRGYAWDVVNEAIEDKTDQYLRDTRWLEIIGEDYLRQAFEMAHQADPDALLFYNDYNE 184 Y+G+ Y WDV+NEA+ D+ D+ LR ++W +IIG+D++ QAF A++ADPDALLFYNDYNE Sbjct: 122 YKGKIYCWDVINEAVADEGDELLRPSKWRQIIGDDFMEQAFLYAYEADPDALLFYNDYNE 181 Query: 185 TDPVKSGKIYKLVRSLLDQNTPIHGIGMQGHWNIYGPSIEEIRNALKLYASLGLKIHVTE 244 P K KI+ LV+SL D+ PIHGIGMQ HW++ PS++EIR A++ YASLG+ +H+TE Sbjct: 182 CFPEKREKIFALVKSLRDKGIPIHGIGMQAHWSLTRPSLDEIRAAIERYASLGVVLHITE 241 Query: 245 LDLSVFRHDDKRTDLKAPTDEMLKLQEERYAEIFALLLEFRDSIDSVTFWGVADDYTWLD 304 LD+S+F D+RTDL APT EM++ Q ERY +IFAL E+RD I SVTFWG+ADD+TWLD Sbjct: 242 LDVSMFEFHDRRTDLAAPTSEMIERQAERYGQIFALFKEYRDVIQSVTFWGIADDHTWLD 301 Query: 305 GFPVRGRKNWPFLFDEQKQPKASFAR 330 FPV GRKNWP LFDEQ +PK +F R Sbjct: 302 NFPVHGRKNWPLLFDEQHKPKPAFWR 327 >pir||I40356 endo-beta-1,4-xylanase - Bacillus sp emb|CAA84631.1| endo-beta-1,4-xylanase [Bacillus sp.] Length = 331 Score = 411 bits (1056), Expect = e-113, Method: Compositional matrix adjust. Identities = 176/321 (54%), Positives = 249/321 (77%) Query: 8 IPALHEMFREAFKIGAAVSTGIIAAQGPFIARHYNSITAENEMKPALVQPEEGEFTFEAA 67 IP L+E++++ F IGAAV++ + ++ + +HYNS+TAENEMK L+QPE+G F F A Sbjct: 7 IPNLYEIYKDFFSIGAAVNSKTLESEKELLKKHYNSLTAENEMKFELLQPEQGNFNFTQA 66 Query: 68 DGIFEFAESHGIGVRGHTLLWHNQTGDWMFREAEGGTCSREQLLARLQTHINTVVGRYRG 127 D + FA H + +RGHTL+WHNQT W+F+ ++G +RE LL R++ HI+TV+GRY+G Sbjct: 67 DKLVAFANEHNMKLRGHTLVWHNQTTGWLFQNSDGTQVNRETLLQRMEAHISTVLGRYKG 126 Query: 128 RGYAWDVVNEAIEDKTDQYLRDTRWLEIIGEDYLRQAFEMAHQADPDALLFYNDYNETDP 187 + Y+WDVVNEAI D +YLR ++WL+IIGED++ +AFE AHQADP+A LFYNDYNE+ P Sbjct: 127 QFYSWDVVNEAISDDDSEYLRKSKWLDIIGEDFIAKAFEFAHQADPNASLFYNDYNESHP 186 Query: 188 VKSGKIYKLVRSLLDQNTPIHGIGMQGHWNIYGPSIEEIRNALKLYASLGLKIHVTELDL 247 K +IY+LV+SLLD++ PIHG+G+Q HWN++ PS+++IR A++ YASLG+++ +TE+D+ Sbjct: 187 NKRERIYRLVKSLLDKDVPIHGVGLQAHWNVHDPSLDDIRAAIERYASLGIQLQITEMDV 246 Query: 248 SVFRHDDKRTDLKAPTDEMLKLQEERYAEIFALLLEFRDSIDSVTFWGVADDYTWLDGFP 307 S+F D++R DL PT++ML LQEERY + F L E++D I +VTFWG D YTWL+ FP Sbjct: 247 SMFSWDNRRADLTQPTEKMLNLQEERYDQFFQLFREYKDVISNVTFWGANDSYTWLNDFP 306 Query: 308 VRGRKNWPFLFDEQKQPKASF 328 VRGRKNWPF+FDE+ P+ F Sbjct: 307 VRGRKNWPFIFDEKGDPQRVF 327 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 515,631,684 Number of extensions: 21822606 Number of successful extensions: 50118 Number of sequences better than 1.0e-05: 361 Number of HSP's gapped: 49088 Number of HSP's successfully gapped: 361 Length of query: 339 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 213 Effective length of database: 415,135,104 Effective search space: 88423777152 Effective search space used: 88423777152 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)