BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0207.0015.p (460 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_243072.1| oxoglutarate dehydrogenase [Bacillus halodurans... 530 e-149 ref|NP_692010.1| oxoglutarate dehydrogenase E1 subunit [Oceanoba... 494 e-138 ref|NP_977701.1| 2-oxoglutarate dehydrogenase, E1 component [Bac... 493 e-138 ref|NP_655162.1| E1_dehydrog, Dehydrogenase E1 component [Bacill... 492 e-138 ref|NP_831036.1| 2-oxoglutarate dehydrogenase E1 component [Baci... 489 e-137 >ref|NP_243072.1| oxoglutarate dehydrogenase [Bacillus halodurans] pir||F83925 oxoglutarate dehydrogenase BH2206 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05925.1| oxoglutarate dehydrogenase [Bacillus halodurans] Length = 945 Score = 530 bits (1365), Expect = e-149, Method: Compositional matrix adjust. Identities = 262/464 (56%), Positives = 333/464 (71%), Gaps = 7/464 (1%) Query: 1 MAVKEPYNESIWSKYHGPNLGYIQEKYEQFVKDPSSVEHHYRDLFTVSGXXXXXXXXXXX 60 M+ KE +E W ++GPNLG E YE++ KDP++V+ R+ F G Sbjct: 1 MSSKEHTSEEPWQGFYGPNLGVAVELYEEYQKDPNAVDDELREAFEKWGPPPATAPERTQ 60 Query: 61 XXXXLSGGTE--WLRKAVRASKLIANIRIYGHMAANIDPL--EQGNNPMAKWLDYETYDL 116 + T+ ++K V A KL +IR +GH+AA+I P+ EQ M D + + L Sbjct: 61 STFTAASITDPDVIKKFVGAVKLADHIRAFGHLAADIQPILKEQRREDM---FDLDRFGL 117 Query: 117 TREDLIAMPASLIWENAPADVHTAWDAVHRMLQAYTQTIAYEFGHVHDEQELRWLNSQAE 176 T D+ ++P L+ APA V DA++ + + YT+TIA+EF HV DE+E +WLN E Sbjct: 118 TESDIRSVPVDLLCPYAPAHVKNGLDAINHLKEVYTKTIAFEFVHVTDEEERKWLNRMIE 177 Query: 177 STSSPAPLNNAERKELLNRLIQVEQFETFLHKTFVGQKRFSLEGNDALVPMLDEIVRAAA 236 S L+ +RK LL RL VE FE FLH+TFVGQKRFS+EG D LVPMLDE+VR A Sbjct: 178 SGLYLPNLSTEQRKSLLKRLTDVEGFEKFLHRTFVGQKRFSIEGLDTLVPMLDEVVREAV 237 Query: 237 HDGAEHILMGMAHRGRLNVLAHILGKPYDIIFSEFHHSPNKELFPSEGSMGINYGWTGDV 296 H+G ++++GMAHRGRLNVLAH+L KPY++IF+EF HS NK+LFPSEGS+GINYGWTGDV Sbjct: 238 HEGTTNVMIGMAHRGRLNVLAHVLNKPYEMIFAEFLHSLNKDLFPSEGSIGINYGWTGDV 297 Query: 297 KYHLGADRAVREGETVRTRITLANNPSHLEFVNPVVEGFARAAQEDRNSPGLPQLDTNKA 356 KYHLGADR +R+ T R+TLANNPSHLEFV+P+VEG+ARAAQEDR SPG P+ D KA Sbjct: 298 KYHLGADRQIRDENTAEARLTLANNPSHLEFVSPIVEGYARAAQEDRTSPGAPEQDIMKA 357 Query: 357 MAVLMHGDAAFPGEGIVAETLNIGKLKGYSNGGTVHIIVNNRIGFTTESEDSRSTHYASD 416 ++L+HGDAAFPG+G+V ETLN+ +L GY GG++HII NN IGFTTE+ DSRST Y+SD Sbjct: 358 YSILIHGDAAFPGQGVVTETLNLSRLTGYQTGGSLHIIANNNIGFTTETFDSRSTLYSSD 417 Query: 417 LAKGYEIPIVHVNADDPEACIAAVRLASAYRSQFKKDFVIDLIG 460 AKG+EIPIVHVNADDPEAC+AAV LA YR +FKKDF+IDLIG Sbjct: 418 PAKGFEIPIVHVNADDPEACLAAVHLAFQYRKRFKKDFLIDLIG 461 >ref|NP_692010.1| oxoglutarate dehydrogenase E1 subunit [Oceanobacillus iheyensis HTE831] dbj|BAC13045.1| oxoglutarate dehydrogenase E1 subunit (alpha-ketoglutarte dehydrogenase) [Oceanobacillus iheyensis HTE831] Length = 953 Score = 494 bits (1271), Expect = e-138, Method: Compositional matrix adjust. Identities = 241/461 (52%), Positives = 324/461 (70%), Gaps = 3/461 (0%) Query: 1 MAVKEPYNESIWSKYHGPNLGYIQEKYEQFVKDPSSVEHHYRDLFTVSGXXXXXXXXXXX 60 MA E W ++HG N GY+++++E + +DP VE R +F G Sbjct: 1 MAENAESAERFWGQFHGQNTGYLEQQFELYKEDPELVESSIRTIFDTHGAPSWLSSTENV 60 Query: 61 XXXXLSGGTEW-LRKAVRASKLIANIRIYGHMAANIDPLEQGNNPMAKWLDYETYDLTRE 119 +S +++ + K A +L+ IR YGH A+I P+ +K LD TY+L + Sbjct: 61 KS--VSNASDFDVTKLTSAIRLVEAIRRYGHTDADIYPVGGYKGDRSKMLDLSTYNLKEQ 118 Query: 120 DLIAMPASLIWENAPADVHTAWDAVHRMLQAYTQTIAYEFGHVHDEQELRWLNSQAESTS 179 DL +PAS IWE V TA D V+++ + YT TI +E+ HV++++E +WL E + Sbjct: 119 DLEKIPASWIWEKQAPGVATALDVVNQLKKYYTGTITFEYDHVNNDEERKWLFDLIEEGN 178 Query: 180 SPAPLNNAERKELLNRLIQVEQFETFLHKTFVGQKRFSLEGNDALVPMLDEIVRAAAHDG 239 + ++ ERK++L RL VE FE FLHKTFVGQKRFS+EG +++VPM+D IV+ + D Sbjct: 179 ARLDPSDDERKKILQRLADVEGFEKFLHKTFVGQKRFSIEGLESMVPMIDHIVQYSNQDS 238 Query: 240 AEHILMGMAHRGRLNVLAHILGKPYDIIFSEFHHSPNKELFPSEGSMGINYGWTGDVKYH 299 EH++MGMAHRGRL+VLA++LGKPYD IFSEF+++ KEL PSEGS INYGWTGDVKYH Sbjct: 239 IEHVMMGMAHRGRLSVLANVLGKPYDKIFSEFNYTKEKELMPSEGSRAINYGWTGDVKYH 298 Query: 300 LGADRAVREGETVRTRITLANNPSHLEFVNPVVEGFARAAQEDRNSPGLPQLDTNKAMAV 359 GA++ V G +TRITLA+NPSHLEFVNPVVEGF RAAQ+DR+ G P+ TNKA++V Sbjct: 299 YGAEKEVEFGNKGQTRITLAHNPSHLEFVNPVVEGFTRAAQDDRSEKGYPKQVTNKAVSV 358 Query: 360 LMHGDAAFPGEGIVAETLNIGKLKGYSNGGTVHIIVNNRIGFTTESEDSRSTHYASDLAK 419 L+HGDAAF GEG+VAETLN+ L GYS GGT+HII NN +G+TT+ ED RST YASDLAK Sbjct: 359 LIHGDAAFIGEGVVAETLNLSGLPGYSTGGTLHIIANNLLGYTTDREDGRSTRYASDLAK 418 Query: 420 GYEIPIVHVNADDPEACIAAVRLASAYRSQFKKDFVIDLIG 460 G+EIP++ VNADDP +CI+A+++A YR +F+KDF+IDL+G Sbjct: 419 GFEIPVIRVNADDPISCISAIKIAYEYRQKFQKDFLIDLVG 459 >ref|NP_977701.1| 2-oxoglutarate dehydrogenase, E1 component [Bacillus cereus ATCC 10987] gb|AAS40309.1| 2-oxoglutarate dehydrogenase, E1 component [Bacillus cereus ATCC 10987] Length = 955 Score = 493 bits (1270), Expect = e-138, Method: Compositional matrix adjust. Identities = 243/452 (53%), Positives = 315/452 (69%), Gaps = 12/452 (2%) Query: 12 WSKYHGPNLGYIQEKYEQFVKDPSSVEHHYRDLFTVSGXXXXXXXXXXXXXXXL---SGG 68 W+K+HGPNLGY+ E+Y+ +V SV+ ++LF + G Sbjct: 11 WAKFHGPNLGYVIEQYDLYVTGAGSVDPELQELFEIFGAPSFQDDVVTGDNTATHFSPQN 70 Query: 69 TEWLRKAVRASKLIANIRIYGHMAANIDPLEQGNNPMAKWLDYETYDLTREDLIAMPASL 128 T + K ++ +L+ IR +GH A+I+P+E N + L+ +L+ DL A+PA Sbjct: 71 TGNIEKILKVVQLVEQIRSFGHTLAHINPMEDAANGQS-LLEKAMNELSDADLKAIPAKT 129 Query: 129 IWENAPADVHTAWDAVHRMLQAYTQTIAYEFGHVHDEQELRWLNSQAESTSSPAPLNNAE 188 +W++AP +HTA D +HR+ + YTQT+AYEF H+ D +E WL+ ES S PL+N + Sbjct: 130 VWQDAPEGIHTALDVIHRLKEVYTQTLAYEFSHIQDSEERAWLHQMVESNSLRQPLSNKK 189 Query: 189 RKELLNRLIQVEQFETFLHKTFVGQKRFSLEGNDALVPMLDEIVRAAAHDGAEHILMGMA 248 R LL RL VE FE FLHKTFVGQKRFS+EG D LVP+LDEIV A +G E +++GMA Sbjct: 190 RTALLKRLTAVEGFEQFLHKTFVGQKRFSIEGVDMLVPVLDEIVLEGAKNGVEDVMIGMA 249 Query: 249 HRGRLNVLAHILGKPYDIIFSEFHHSPNKELFPSEGSMGINYGWTGDVKYHLGADRAVRE 308 HRGRL+VLAH+L KPY +F+EF H+ EG++ N GWTGDVKYHLG ++ V Sbjct: 250 HRGRLSVLAHVLEKPYSHMFAEFKHAK------IEGAVA-NSGWTGDVKYHLGREQVV-S 301 Query: 309 GETVRTRITLANNPSHLEFVNPVVEGFARAAQEDRNSPGLPQLDTNKAMAVLMHGDAAFP 368 E V TR+TLANNPSHLEFVNPVVEGFARAAQE+R GLP+ DT+K+ +L+HGDAAFP Sbjct: 302 NEEVSTRVTLANNPSHLEFVNPVVEGFARAAQENRKKSGLPEQDTSKSFVILVHGDAAFP 361 Query: 369 GEGIVAETLNIGKLKGYSNGGTVHIIVNNRIGFTTESEDSRSTHYASDLAKGYEIPIVHV 428 G+GIV+ETLN+ +L Y GGT+H+I NN +GFTT+S DSRST Y+SDLAKG++IPIVHV Sbjct: 362 GQGIVSETLNLSRLNAYQTGGTIHVIANNAVGFTTDSYDSRSTKYSSDLAKGFDIPIVHV 421 Query: 429 NADDPEACIAAVRLASAYRSQFKKDFVIDLIG 460 NADDPEAC+AA LA YR FKKDF+IDLIG Sbjct: 422 NADDPEACLAAANLAIQYRMLFKKDFLIDLIG 453 >ref|NP_655162.1| E1_dehydrog, Dehydrogenase E1 component [Bacillus anthracis A2012] ref|NP_843742.1| 2-oxoglutarate dehydrogenase, E1 component [Bacillus anthracis str. Ames] gb|AAP25228.1| 2-oxoglutarate dehydrogenase, E1 component [Bacillus anthracis str. Ames] Length = 955 Score = 492 bits (1266), Expect = e-138, Method: Compositional matrix adjust. Identities = 242/452 (53%), Positives = 315/452 (69%), Gaps = 12/452 (2%) Query: 12 WSKYHGPNLGYIQEKYEQFVKDPSSVEHHYRDLFTVSGXXXXXXXXXXXXXXXL---SGG 68 W+K+HGPNLGY+ E+Y+ +V SV+ ++LF + G Sbjct: 11 WAKFHGPNLGYVIEQYDLYVTGAGSVDPELQELFEIFGAPSFQDDVVTGDNTATHFSPQN 70 Query: 69 TEWLRKAVRASKLIANIRIYGHMAANIDPLEQGNNPMAKWLDYETYDLTREDLIAMPASL 128 T + K ++ +L+ IR +GH A+I+P+E N + L+ +L+ DL A+PA Sbjct: 71 TGNIEKILKVVQLVEQIRSFGHTLAHINPMEDAANGQS-LLEKAMNELSDADLKAIPAKT 129 Query: 129 IWENAPADVHTAWDAVHRMLQAYTQTIAYEFGHVHDEQELRWLNSQAESTSSPAPLNNAE 188 +W++AP +HTA D +HR+ + YTQ++AYEF H+ D +E WL+ ES S PL+N + Sbjct: 130 VWQDAPEGIHTALDVIHRLKEVYTQSLAYEFSHIQDSEERAWLHQMVESNSLRQPLSNKK 189 Query: 189 RKELLNRLIQVEQFETFLHKTFVGQKRFSLEGNDALVPMLDEIVRAAAHDGAEHILMGMA 248 R LL RL VE FE FLHKTFVGQKRFS+EG D LVP+LDEIV A +G E +++GMA Sbjct: 190 RTALLKRLTAVEGFEQFLHKTFVGQKRFSIEGVDMLVPVLDEIVLEGAKNGVEDVMIGMA 249 Query: 249 HRGRLNVLAHILGKPYDIIFSEFHHSPNKELFPSEGSMGINYGWTGDVKYHLGADRAVRE 308 HRGRL+VLAH+L KPY +F+EF H+ EG++ N GWTGDVKYHLG ++ V Sbjct: 250 HRGRLSVLAHVLEKPYSHMFAEFKHAK------IEGAVA-NSGWTGDVKYHLGREQVV-S 301 Query: 309 GETVRTRITLANNPSHLEFVNPVVEGFARAAQEDRNSPGLPQLDTNKAMAVLMHGDAAFP 368 E V TR+TLANNPSHLEFVNPVVEGFARAAQE+R GLP+ DT+K+ +L+HGDAAFP Sbjct: 302 NEEVSTRVTLANNPSHLEFVNPVVEGFARAAQENRKKSGLPEQDTSKSFVILVHGDAAFP 361 Query: 369 GEGIVAETLNIGKLKGYSNGGTVHIIVNNRIGFTTESEDSRSTHYASDLAKGYEIPIVHV 428 G+GIV+ETLN+ +L Y GGT+H+I NN +GFTT+S DSRST Y+SDLAKG++IPIVHV Sbjct: 362 GQGIVSETLNLSRLNAYQTGGTIHVIANNAVGFTTDSYDSRSTKYSSDLAKGFDIPIVHV 421 Query: 429 NADDPEACIAAVRLASAYRSQFKKDFVIDLIG 460 NADDPEAC+AA LA YR FKKDF+IDLIG Sbjct: 422 NADDPEACLAAANLAIQYRMLFKKDFLIDLIG 453 >ref|NP_831036.1| 2-oxoglutarate dehydrogenase E1 component [Bacillus cereus ATCC 14579] gb|AAP08237.1| 2-oxoglutarate dehydrogenase E1 component [Bacillus cereus ATCC 14579] Length = 955 Score = 489 bits (1258), Expect = e-137, Method: Compositional matrix adjust. Identities = 241/452 (53%), Positives = 313/452 (69%), Gaps = 12/452 (2%) Query: 12 WSKYHGPNLGYIQEKYEQFVKDPSSVEHHYRDLFTVSGXXXXXXXXXXXXXXXL---SGG 68 W+K+HGPNLGY+ E+Y+ +V SV+ ++LF + G Sbjct: 11 WAKFHGPNLGYVIEQYDLYVTGAGSVDPELQELFEIFGAPSFQDDVVTGDNTATHFSPQN 70 Query: 69 TEWLRKAVRASKLIANIRIYGHMAANIDPLEQGNNPMAKWLDYETYDLTREDLIAMPASL 128 T + K ++ +L+ IR +GH A+I+P+E N + L+ +L+ DL A+PA Sbjct: 71 TGNIEKILKVVQLVEQIRSFGHTLAHINPMEDAANGQS-LLEKAMSELSDADLKAIPAKT 129 Query: 129 IWENAPADVHTAWDAVHRMLQAYTQTIAYEFGHVHDEQELRWLNSQAESTSSPAPLNNAE 188 +W++AP +HTA D +HR+ YT+++AYEF H+ D +E WL+ ES S PL+N + Sbjct: 130 VWQDAPEGIHTALDVIHRLKDVYTKSLAYEFSHIQDSEERAWLHQMVESNSLRQPLSNKK 189 Query: 189 RKELLNRLIQVEQFETFLHKTFVGQKRFSLEGNDALVPMLDEIVRAAAHDGAEHILMGMA 248 R LL RL VE FE FLHKTFVGQKRFS+EG D LVP+LDEIV A +G E +++GMA Sbjct: 190 RTALLKRLTAVEGFEQFLHKTFVGQKRFSIEGVDMLVPVLDEIVLEGAKNGVEDVMIGMA 249 Query: 249 HRGRLNVLAHILGKPYDIIFSEFHHSPNKELFPSEGSMGINYGWTGDVKYHLGADRAVRE 308 HRGRL+VLAH+L KPY +F+EF H+ EG++ N GWTGDVKYHLG ++ V Sbjct: 250 HRGRLSVLAHVLEKPYSHMFAEFKHAK------IEGAVA-NSGWTGDVKYHLGREQVV-S 301 Query: 309 GETVRTRITLANNPSHLEFVNPVVEGFARAAQEDRNSPGLPQLDTNKAMAVLMHGDAAFP 368 E V TR+TLANNPSHLEFVNPVVEGFARAAQE+R GLP DT+K+ +L+HGDAAFP Sbjct: 302 NEEVSTRVTLANNPSHLEFVNPVVEGFARAAQENRKKSGLPDQDTSKSFVILVHGDAAFP 361 Query: 369 GEGIVAETLNIGKLKGYSNGGTVHIIVNNRIGFTTESEDSRSTHYASDLAKGYEIPIVHV 428 G+GIV+ETLN+ +L Y GGT+H+I NN +GFTT+S DSRST Y+SDLAKG++IPIVHV Sbjct: 362 GQGIVSETLNLSRLNAYQTGGTIHVIANNAVGFTTDSYDSRSTKYSSDLAKGFDIPIVHV 421 Query: 429 NADDPEACIAAVRLASAYRSQFKKDFVIDLIG 460 NADDPEAC+AA LA YR FKKDF+IDLIG Sbjct: 422 NADDPEACLAAANLAIQYRMLFKKDFLIDLIG 453 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 704,983,554 Number of extensions: 28922274 Number of successful extensions: 65405 Number of sequences better than 1.0e-05: 692 Number of HSP's gapped: 63495 Number of HSP's successfully gapped: 693 Length of query: 460 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 331 Effective length of database: 406,989,807 Effective search space: 134713626117 Effective search space used: 134713626117 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)