BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0203.0002.p (125 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00098101.1| COG1283: Na+/phosphate symporter [Desulfitoba... 96 2e-19 ref|NP_242219.1| BH1353~unknown conserved protein [Bacillus halo... 80 1e-14 ref|NP_987786.1| Na+/Pi-cotransporter related protein [Methanoco... 75 6e-13 ref|NP_390420.1| similar to Na+/Pi cotransporter [Bacillus subti... 73 2e-12 ref|NP_563284.1| conserved hypothetical protein [Clostridium per... 72 6e-12 >ref|ZP_00098101.1| COG1283: Na+/phosphate symporter [Desulfitobacterium hafniense] Length = 287 Score = 96.3 bits (238), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 56/124 (45%), Positives = 76/124 (61%), Gaps = 2/124 (1%) Query: 1 MLHSSAAVIGMAMGLASSGVMPPGLGIAIVLGANIGTCVTAVXXXXXXXXXXKFVAGSHV 60 +LHSSAA G+ M LAS G + IA + GANIGTC TAV + VA HV Sbjct: 166 VLHSSAATTGIVMILASDGCITLPTAIAFIFGANIGTCFTAVIASLATNRAAQRVALFHV 225 Query: 61 ALNIGGALLFLPFIQQLQVLSAWLGGGPAAQLAHAQTIFNVVCSLGVLPLCYLPVWTRLE 120 LN+ GAL+F PF+ L + W+GG + Q+A+A T+FNV+ SL +P +LP+ RL Sbjct: 226 LLNLFGALIFFPFLTPLALFLQWIGGPVSLQVANAHTLFNVLSSLIAMP--FLPLAARLL 283 Query: 121 ERLQ 124 ER++ Sbjct: 284 ERIR 287 >ref|NP_242219.1| BH1353~unknown conserved protein [Bacillus halodurans] pir||A83819 hypothetical protein BH1353 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05072.1| BH1353~unknown conserved protein [Bacillus halodurans] Length = 310 Score = 80.1 bits (196), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 50/110 (45%), Positives = 65/110 (59%) Query: 1 MLHSSAAVIGMAMGLASSGVMPPGLGIAIVLGANIGTCVTAVXXXXXXXXXXKFVAGSHV 60 ++ SS A +AMG + ++ GIAI+LGANIGTC TA+ + VA +H+ Sbjct: 185 LIQSSTATTAIAMGFLNEEIIGIHGGIAIMLGANIGTCFTALLASIGSGRTSRLVAFAHL 244 Query: 61 ALNIGGALLFLPFIQQLQVLSAWLGGGPAAQLAHAQTIFNVVCSLGVLPL 110 LN+ G LLF+P I L L PA QLAHA TIFNV+CSL +LP Sbjct: 245 WLNVIGVLLFIPLIPHLSHFVMTLTTIPAIQLAHASTIFNVICSLAILPF 294 >ref|NP_987786.1| Na+/Pi-cotransporter related protein [Methanococcus maripaludis S2] emb|CAF30222.1| Na+/Pi-cotransporter related protein [Methanococcus maripaludis] Length = 541 Score = 74.7 bits (182), Expect = 6e-13, Method: Composition-based stats. Identities = 46/116 (39%), Positives = 64/116 (55%), Gaps = 9/116 (7%) Query: 1 MLHSSAAVIGMAMGLASSGVMPPGLGIAIVLGANIGTCVTAVXXXXXXXXXXKFVAGSHV 60 M+ SS+A IG+ + +AS+G + + I+ G NIGTCVTA+ K A H+ Sbjct: 185 MIQSSSATIGLLIAVASTGALSLAVAFPILFGDNIGTCVTALLSSIGANRTAKRAALMHL 244 Query: 61 ALNIGGALLFL------PFIQQLQVLSAWLGGGPAAQLAHAQTIFNVVCSLGVLPL 110 NI GAL+F+ P I +Q LSA G Q+A+A TIFNV +L +LP Sbjct: 245 IFNITGALIFMVLIYTTPLISWIQSLSA---GSVERQIANAHTIFNVTNTLIMLPF 297 >ref|NP_390420.1| similar to Na+/Pi cotransporter [Bacillus subtilis] sp|P54463|YQEW_BACSU Hypothetical protein yqeW pir||F69952 Na+/Pi cotransporter homolog yqeW - Bacillus subtilis dbj|BAA12469.1| YqeW [Bacillus subtilis] dbj|BAA12081.1| YqeW [Bacillus subtilis] emb|CAB14484.1| yqeW [Bacillus subtilis subsp. subtilis str. 168] Length = 310 Score = 73.2 bits (178), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 46/125 (36%), Positives = 66/125 (52%), Gaps = 2/125 (1%) Query: 1 MLHSSAAVIGMAMGLASSGVMPPGLGIAIVLGANIGTCVTAVXXXXXXXXXXKFVAGSHV 60 ++HSS+ IG+ M + G++ +++VLG+NIGTC+TAV K A +HV Sbjct: 186 IIHSSSVCIGILMSFMNEGIIGLTQAMSVVLGSNIGTCITAVMAAVSGGYAAKQTAYAHV 245 Query: 61 ALNIGGALLFLPFIQQLQVLSAWLGGGPAAQLAHAQTIFNVVCSLGVLPLCYLPVWTRLE 120 N+ G L LPF+ L PA ++AH +FNVV +L LPL L + RL Sbjct: 246 VFNVLGVALVLPFLTAATGFVEQLSPDPAQKIAHFSLLFNVVTALLFLPLTNL--FYRLI 303 Query: 121 ERLQP 125 L P Sbjct: 304 HLLIP 308 >ref|NP_563284.1| conserved hypothetical protein [Clostridium perfringens] dbj|BAB82074.1| conserved hypothetical protein [Clostridium perfringens str. 13] Length = 537 Score = 71.6 bits (174), Expect = 6e-12, Method: Composition-based stats. Identities = 39/111 (35%), Positives = 63/111 (56%), Gaps = 1/111 (0%) Query: 1 MLHSSAAVIGMAMGLASSGVMPPGLGIAIVLGANIGTCVTAVXXXXXXXXXXKFVAGSHV 60 +L SS+A G+ + LAS+G + + + I+ G NIGTCVTA+ A H+ Sbjct: 188 ILQSSSATTGILIALASTGSITINIVLPILFGCNIGTCVTALIASIGANKTAHKAAAIHL 247 Query: 61 ALNIGGALLFLPFIQQLQVLSAWLGGGPAA-QLAHAQTIFNVVCSLGVLPL 110 NI G L+F+PF++ L + + G Q+A+A TIFN+ ++ ++PL Sbjct: 248 MFNILGTLIFIPFLKPLAYIVQDMSPGDVERQIANAHTIFNITATIILVPL 298 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.140 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 167,856,694 Number of extensions: 5393965 Number of successful extensions: 17229 Number of sequences better than 1.0e-05: 52 Number of HSP's gapped: 17204 Number of HSP's successfully gapped: 52 Length of query: 125 Length of database: 757,237,578 Length adjustment: 89 Effective length of query: 36 Effective length of database: 515,593,767 Effective search space: 18561375612 Effective search space used: 18561375612 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 120 (50.8 bits)