BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0091.p (230 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||E53402 serine O-acetyltransferase (EC 2.3.1.30) cysE [simil... 302 4e-81 ref|NP_829990.1| Serine acetyltransferase [Bacillus cereus ATCC ... 302 4e-81 ref|NP_654037.1| hypothetical protein predicted by GeneMark [Bac... 301 8e-81 ref|NP_387974.1| serine acetyltransferase [Bacillus subtilis] >g... 287 2e-76 ref|NP_240976.1| serine O-acetyltransferase [Bacillus halodurans... 280 2e-74 >pir||E53402 serine O-acetyltransferase (EC 2.3.1.30) cysE [similarity] - Bacillus stearothermophilus Length = 225 Score = 302 bits (774), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 137/224 (61%), Positives = 180/224 (80%), Gaps = 3/224 (1%) Query: 1 MFKHIKSDIRAVFDNDPAARSWFEVVFTYAGLHAIWAHRIAHSFYKRRWFTLARIVSQVS 60 MFK +K DI +F+ DPAARS+ EV+ TY+GLHAIWAHRIAH+ YKR+++ LAR++SQ+S Sbjct: 2 MFKTLKEDIEVIFEQDPAARSYLEVILTYSGLHAIWAHRIAHALYKRKFYFLARLISQIS 61 Query: 61 RFMTGVEIHPGATIGSRLFIDHGMGIVIGETCEIGDDVIIYQGVTLGGTGKEKGKRHPTV 120 RF TG+EIHPGA IG R FIDHGMG+VIGETCEIGD+V +YQGVTLGGTGKEKGKRHPT+ Sbjct: 62 RFFTGIEIHPGAKIGRRFFIDHGMGVVIGETCEIGDNVTVYQGVTLGGTGKEKGKRHPTI 121 Query: 121 GNNVVIGSGAKVLGSFQIGDNCNIGSNAVVLREVPSNSTVVGNPGRVVKRNGERVSDRLD 180 +N +I +GAKVLGS IG+N IG+ +VVL++VP NSTVVG PGRVV R+G +V L+ Sbjct: 122 KDNCLIAAGAKVLGSITIGENSKIGAGSVVLKDVPPNSTVVGIPGRVVVRDGVKVKKDLN 181 Query: 181 HTKMPDPLVDSLRFLQKEIEEIREQLGTEDKQKKEQRRLESQQY 224 HT +PDP+ D R L++EI ++ +L + K+++R+ E +Q+ Sbjct: 182 HTDLPDPIADRFRELEEEIVRLKSEL---EALKQQERKSEYEQH 222 >ref|NP_829990.1| Serine acetyltransferase [Bacillus cereus ATCC 14579] gb|AAP07191.1| Serine acetyltransferase [Bacillus cereus ATCC 14579] Length = 221 Score = 302 bits (773), Expect = 4e-81, Method: Compositional matrix adjust. Identities = 138/221 (62%), Positives = 179/221 (80%), Gaps = 2/221 (0%) Query: 1 MFKHIKSDIRAVFDNDPAARSWFEVVFTYAGLHAIWAHRIAHSFYKRRWFTLARIVSQVS 60 MFK ++ DI VF+ DPAAR +FEV+ TY+GLHA+WAHRIAH+FYK+ +F LAR VSQVS Sbjct: 1 MFKRLREDIEVVFEQDPAARGYFEVILTYSGLHAVWAHRIAHAFYKKNFFFLARFVSQVS 60 Query: 61 RFMTGVEIHPGATIGSRLFIDHGMGIVIGETCEIGDDVIIYQGVTLGGTGKEKGKRHPTV 120 RF TG+EIHPGATIG R FIDHGMG+VIGETCEIGD+V IYQGVTLGGTGKEKGKRHPT+ Sbjct: 61 RFFTGIEIHPGATIGRRFFIDHGMGVVIGETCEIGDNVTIYQGVTLGGTGKEKGKRHPTI 120 Query: 121 GNNVVIGSGAKVLGSFQIGDNCNIGSNAVVLREVPSNSTVVGNPGRVVKRNGERVSDRLD 180 +NV+I +GAKVLGS +G+N IG+ +VVL+EVP++STVVG PGRVV +NG ++ L+ Sbjct: 121 QDNVLIATGAKVLGSITVGENSKIGAGSVVLKEVPAHSTVVGIPGRVVIQNGVKIGQELN 180 Query: 181 HTKMPDPLVDSLRFLQKEIEEIREQ--LGTEDKQKKEQRRL 219 H+ +PDP+ D L+ ++ E++++++Q L E K K + L Sbjct: 181 HSDLPDPIFDKLKAMEVELDKLKKQLELKVERKDKNDYSHL 221 >ref|NP_654037.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_842656.1| serine O-acetyltransferase [Bacillus anthracis str. Ames] ref|NP_976416.1| serine O-acetyltransferase [Bacillus cereus ATCC 10987] gb|AAP24142.1| serine O-acetyltransferase [Bacillus anthracis str. Ames] gb|AAS39024.1| serine O-acetyltransferase [Bacillus cereus ATCC 10987] Length = 221 Score = 301 bits (771), Expect = 8e-81, Method: Compositional matrix adjust. Identities = 134/213 (62%), Positives = 179/213 (84%) Query: 1 MFKHIKSDIRAVFDNDPAARSWFEVVFTYAGLHAIWAHRIAHSFYKRRWFTLARIVSQVS 60 MFK ++ DI VF+ DPAARS+FEV+ TY+GLHA+WAHRIAH+FYK+ +F +AR +SQVS Sbjct: 1 MFKRLREDIEVVFEQDPAARSYFEVILTYSGLHAVWAHRIAHAFYKKNFFFIARWISQVS 60 Query: 61 RFMTGVEIHPGATIGSRLFIDHGMGIVIGETCEIGDDVIIYQGVTLGGTGKEKGKRHPTV 120 RF TG+EIHPGATIG R FIDHGMG+VIGETCEIGD+V IYQGVTLGGTGKEKGKRHPT+ Sbjct: 61 RFFTGIEIHPGATIGRRFFIDHGMGVVIGETCEIGDNVTIYQGVTLGGTGKEKGKRHPTI 120 Query: 121 GNNVVIGSGAKVLGSFQIGDNCNIGSNAVVLREVPSNSTVVGNPGRVVKRNGERVSDRLD 180 +NV+I +GAKVLGS +G+N IG+ +VVL+EVP++STVVG PGRVV +NG ++ L+ Sbjct: 121 QDNVLIATGAKVLGSITVGENSKIGAGSVVLKEVPAHSTVVGIPGRVVIQNGVKIGQELN 180 Query: 181 HTKMPDPLVDSLRFLQKEIEEIREQLGTEDKQK 213 H+ +PDP+ D L+ ++ E++++++QL + ++K Sbjct: 181 HSDLPDPIFDKLKVMEVELDKLKKQLEVKVERK 213 >ref|NP_387974.1| serine acetyltransferase [Bacillus subtilis] sp|Q06750|CYSE_BACSU Serine acetyltransferase (SAT) pir||B53402 serine O-acetyltransferase (EC 2.3.1.30) cysE [validated] - Bacillus subtilis gb|AAA21797.1| serine acetyltransferase dbj|BAA05327.1| serine acetyltransferase [Bacillus subtilis] emb|CAB11869.1| serine acetyltransferase [Bacillus subtilis subsp. subtilis str. 168] Length = 217 Score = 287 bits (734), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 130/214 (60%), Positives = 174/214 (81%), Gaps = 2/214 (0%) Query: 1 MFKHIKSDIRAVFDNDPAARSWFEVVFTYAGLHAIWAHRIAHSFYKRRWFTLARIVSQVS 60 F+ +K DI VFD DPAARS+FEV+ TY+GLHAIWAHRIAH+ YKR+++ LAR++SQVS Sbjct: 2 FFRMLKEDIDTVFDQDPAARSYFEVILTYSGLHAIWAHRIAHALYKRKFYFLARLISQVS 61 Query: 61 RFMTGVEIHPGATIGSRLFIDHGMGIVIGETCEIGDDVIIYQGVTLGGTGKEKGKRHPTV 120 RF TG+EIHPGATIG R FIDHGMG+VIGETCEIG++V ++QGVTLGGTGKEKGKRHPT+ Sbjct: 62 RFFTGIEIHPGATIGRRFFIDHGMGVVIGETCEIGNNVTVFQGVTLGGTGKEKGKRHPTI 121 Query: 121 GNNVVIGSGAKVLGSFQIGDNCNIGSNAVVLREVPSNSTVVGNPGRVVKRNGERVSDRLD 180 ++ +I +GAKVLGS +G+ IG+ +VVL +VP STVVG PGRVV +NG++V L+ Sbjct: 122 KDDALIATGAKVLGSITVGEGSKIGAGSVVLHDVPDFSTVVGIPGRVVVQNGKKVRRDLN 181 Query: 181 HTKMPDPLVDSLRFLQKEIEEIREQLGTEDKQKK 214 H +PDP+ D + L+++I E++ +L ED++++ Sbjct: 182 HQDLPDPVADRFKSLEQQILELKAEL--EDRKER 213 >ref|NP_240976.1| serine O-acetyltransferase [Bacillus halodurans] pir||F83663 serine O-acetyltransferase cysE [imported] - Bacillus halodurans (strain C-125) dbj|BAB03829.1| serine O-acetyltransferase [Bacillus halodurans] Length = 229 Score = 280 bits (717), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 131/206 (63%), Positives = 169/206 (82%) Query: 1 MFKHIKSDIRAVFDNDPAARSWFEVVFTYAGLHAIWAHRIAHSFYKRRWFTLARIVSQVS 60 +FK + +DI VF+ DPAARS EV+FTY+G+HAIWAHR+AH ++++ + +AR +SQVS Sbjct: 4 IFKTLLNDIDVVFEQDPAARSRLEVIFTYSGVHAIWAHRLAHWLWRKKLYFVARFLSQVS 63 Query: 61 RFMTGVEIHPGATIGSRLFIDHGMGIVIGETCEIGDDVIIYQGVTLGGTGKEKGKRHPTV 120 R +TG+EIHPGA IG RLFIDHGMG+VIGETCEIGD+V IYQGVTLGGTGKEKGKRHPTV Sbjct: 64 RLITGIEIHPGAKIGQRLFIDHGMGVVIGETCEIGDNVTIYQGVTLGGTGKEKGKRHPTV 123 Query: 121 GNNVVIGSGAKVLGSFQIGDNCNIGSNAVVLREVPSNSTVVGNPGRVVKRNGERVSDRLD 180 ++V+I SGAKVLGSF IG N IG+ +VVL EVP NSTVVG PG+VV R+G +V+ LD Sbjct: 124 EDHVLIASGAKVLGSFTIGKNSRIGAGSVVLNEVPPNSTVVGIPGKVVIRDGIKVNRDLD 183 Query: 181 HTKMPDPLVDSLRFLQKEIEEIREQL 206 H +PDP+ D R L+K++E +++++ Sbjct: 184 HHLLPDPVSDKFRELEKQLEALQKEI 209 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 361,659,348 Number of extensions: 15918849 Number of successful extensions: 60204 Number of sequences better than 1.0e-05: 921 Number of HSP's gapped: 59395 Number of HSP's successfully gapped: 921 Length of query: 230 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 109 Effective length of database: 428,710,599 Effective search space: 46729455291 Effective search space used: 46729455291 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 124 (52.4 bits)