BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0188.0045.p (432 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_241020.1| preprotein translocase subunit [Bacillus halodu... 509 e-143 ref|NP_654073.1| secY, eubacterial secY protein [Bacillus anthra... 499 e-140 pir||B44859 preprotein translocase secY - Bacillus sp >gi|484251... 496 e-139 ref|NP_691060.1| preprotein translocase subunit [Oceanobacillus ... 495 e-139 ref|NP_388017.1| preprotein translocase subunit [Bacillus subtil... 482 e-135 >ref|NP_241020.1| preprotein translocase subunit [Bacillus halodurans] sp|P38375|SECY_BACHD Preprotein translocase secY subunit pir||T44403 preprotein translocase subunit secY [imported] - Bacillus halodurans (strain C-125) dbj|BAA75291.1| secY homologue (identity of 70% to B. subtilis ) [Bacillus halodurans] dbj|BAB03873.1| preprotein translocase subunit [Bacillus halodurans] Length = 430 Score = 509 bits (1311), Expect = e-143, Method: Compositional matrix adjust. Identities = 256/434 (58%), Positives = 322/434 (74%), Gaps = 7/434 (1%) Query: 1 MFKTLKNIWHIEDLRKKILFTLFVLIIYRIGSFVPVPGVNKEVLESTNQGGEALMGLLNT 60 MF+T+ NI+ + DLR+K++FTL +LI++RIGSF+PVPG N+EVL+ +Q G LNT Sbjct: 1 MFRTISNIFRVGDLRRKVIFTLLMLIVFRIGSFIPVPGTNREVLDFVDQANA--FGFLNT 58 Query: 61 FSGGALKNFSIFAISIYPYITASIIVQLLSMDVVPKFAEWAKQGEHGKKQLAQITRYGTV 120 F GGAL NFSIFA+ I PYITASI++QLL MDVVPKFAEWAK+GE G+++LAQ TRYGT+ Sbjct: 59 FGGGALGNFSIFAMGIMPYITASIVMQLLQMDVVPKFAEWAKEGEAGRRKLAQFTRYGTI 118 Query: 121 VLGLIQAFATSIGFNRIYGTEMIPNATFADYLLIAIILTAGTSFLMWLGEQITEKGIGNG 180 VLG IQA S+GFN + +IPN + + YL IA++LTAGT+FLMWLGEQIT KG+GNG Sbjct: 119 VLGFIQALGMSVGFNNFF-PGLIPNPSVSVYLFIALVLTAGTAFLMWLGEQITAKGVGNG 177 Query: 181 ISILIFAGIVAAIPGYITTTAQSSFIQP--DQXXXXXXXXXXXXXXXXXXXXXXXXXQQG 238 ISI+IFAGI A IP + S+ IQ +Q QQ Sbjct: 178 ISIIIFAGIAAGIPNGLNLI-YSTRIQDAGEQLFLNIVVILLLALAILAIIVGVIFVQQA 236 Query: 239 IRKIPVQYAKRVVGNKMYGGQNTHIPLKINAAGVIPVIFAVSLLQFPIVISS-FWSTHEW 297 +RKIPVQYAKR+VG GGQ+TH+PLK+NAAGVIPVIFA+SL FP ++ F S H Sbjct: 237 LRKIPVQYAKRLVGRNPVGGQSTHLPLKVNAAGVIPVIFALSLFIFPPTVAGLFGSDHPV 296 Query: 298 AKWVTNNLAHDKPLGMVLYVIMIIGFTFFYTFVQMNPQQMADNMKKNGGYIPGIRPGKAT 357 A WV + P+GM +Y ++IIGFT+FYTF+Q+NP++MA+N+KK GGYIPGIRPGKAT Sbjct: 297 AAWVIETFDYTHPIGMAVYALLIIGFTYFYTFIQVNPERMAENLKKQGGYIPGIRPGKAT 356 Query: 358 EKYLTRVMSRLTMSGALFLAVISILPVLFGSLSGLPRQVQIGGTALLIVVGVALDTMKQI 417 + Y+TR++ RLT G+LFLAV++ILPV F + LP+ +QIGGT LLIVVGVALDTMKQI Sbjct: 357 QTYITRILYRLTFVGSLFLAVVAILPVFFIKFADLPQAIQIGGTGLLIVVGVALDTMKQI 416 Query: 418 ESQLIKRHYKGFIN 431 E+QLIKR YKGFI Sbjct: 417 EAQLIKRSYKGFIK 430 >ref|NP_654073.1| secY, eubacterial secY protein [Bacillus anthracis A2012] ref|NP_842698.1| preprotein translocase, SecY subunit [Bacillus anthracis str. Ames] ref|NP_976458.1| preprotein translocase, SecY subunit [Bacillus cereus ATCC 10987] gb|AAP24184.1| preprotein translocase, SecY subunit [Bacillus anthracis str. Ames] gb|AAS39066.1| preprotein translocase, SecY subunit [Bacillus cereus ATCC 10987] Length = 433 Score = 499 bits (1286), Expect = e-140, Method: Compositional matrix adjust. Identities = 249/435 (57%), Positives = 319/435 (73%), Gaps = 5/435 (1%) Query: 1 MFKTLKNIWHIEDLRKKILFTLFVLIIYRIGSFVPVPGVNKEVLESTNQGGEALMGLLNT 60 MF+T+ N + ++R+KILFTL +LI++RIG+F+PVP N +VL++ +Q +G+LNT Sbjct: 1 MFRTISNFMRVAEIRRKILFTLAMLIVFRIGTFIPVPFTNGDVLKAQDQLNA--LGILNT 58 Query: 61 FSGGALKNFSIFAISIYPYITASIIVQLLSMDVVPKFAEWAKQGEHGKKQLAQITRYGTV 120 F GGALKNFSIFA+ I PYITASIIVQLL MDVVPKF+EWAKQGE G+++L Q TRY T+ Sbjct: 59 FGGGALKNFSIFAMGIMPYITASIIVQLLQMDVVPKFSEWAKQGEMGRRKLTQFTRYFTI 118 Query: 121 VLGLIQAFATSIGFNRIYGTEMIPNATFADYLLIAIILTAGTSFLMWLGEQITEKGIGNG 180 VL IQ F SIGFN + G ++I N ++ YL IA +LTAGT+FLMWLGEQIT KG+GNG Sbjct: 119 VLAFIQGFGMSIGFNGMVGGQLILNPGWSTYLYIATVLTAGTAFLMWLGEQITAKGVGNG 178 Query: 181 ISILIFAGIVAAIPGYITTTAQSSFIQ-PDQXXXXXXXXXXXXXXXXXXXXXXXXXQQGI 239 ISILIF GI AAIP I+ Q F DQ QQ + Sbjct: 179 ISILIFGGIAAAIPSVISQVYQQQFQNIGDQLFMSIVKVALILLAVLAVIVGVIFIQQAV 238 Query: 240 RKIPVQYAKRVVG-NKMYGG-QNTHIPLKINAAGVIPVIFAVSLLQFPIVISSFWSTHEW 297 RKIP+QYAKRV G N Y G QNTH+PLK+N+AGVIPVIFAVS L P I+ F+ H+ Sbjct: 239 RKIPIQYAKRVTGGNGGYAGAQNTHLPLKVNSAGVIPVIFAVSFLITPPTIAQFFPKHDV 298 Query: 298 AKWVTNNLAHDKPLGMVLYVIMIIGFTFFYTFVQMNPQQMADNMKKNGGYIPGIRPGKAT 357 ++W+ N + P+GM++YV +I+ FT+FY FVQ+NP+QM++N+ K GGY+PGIRPGK T Sbjct: 299 SQWIIANFNYSHPVGMIIYVALIVAFTYFYAFVQVNPEQMSENLNKQGGYVPGIRPGKNT 358 Query: 358 EKYLTRVMSRLTMSGALFLAVISILPVLFGSLSGLPRQVQIGGTALLIVVGVALDTMKQI 417 E+YLT+++ RLT G++FLA I+ILPV+F L LP QIGGT++LIVVGVAL+TMKQ+ Sbjct: 359 EQYLTKILYRLTFVGSIFLAAIAILPVIFTKLGNLPPSAQIGGTSMLIVVGVALETMKQL 418 Query: 418 ESQLIKRHYKGFINK 432 ESQL+KRHYKGFI + Sbjct: 419 ESQLVKRHYKGFIKQ 433 >pir||B44859 preprotein translocase secY - Bacillus sp dbj|BAA01191.1| secretion protein Y [Bacillus sp.] Length = 431 Score = 496 bits (1278), Expect = e-139, Method: Compositional matrix adjust. Identities = 255/435 (58%), Positives = 319/435 (73%), Gaps = 8/435 (1%) Query: 1 MFKTLKNIWHIEDLRKKILFTLFVLIIYRIGSFVPVPGVNKEVLESTNQGGEALMGLLNT 60 MF+T+ NI+ + DLR+K++FTL +LI++RIGSF+PVPG N+EVL+ +Q G LNT Sbjct: 1 MFRTISNIFRVGDLRRKVIFTLLMLIVFRIGSFIPVPGTNREVLDFVDQANA--FGFLNT 58 Query: 61 FSGGALKNFSIFAISIYPYITASIIVQLLSMDVVPKFAEWAKQGEHGKKQLAQITRYGT- 119 F GGAL NFSIFA+ I PYITASI++QLL MDVVPKFAEWAK+GE G+++LAQ TRYGT Sbjct: 59 FGGGALGNFSIFAMGIMPYITASIVMQLLQMDVVPKFAEWAKEGEAGRRKLAQFTRYGTI 118 Query: 120 VVLGLIQAFATSIGFNRIYGTEMIPNATFADYLLIAIILTAGTSFLMWLGEQITEKGIGN 179 VVLG IQA S+GFN + +IPN + + YL IA++LTAGT+FLMWLGEQIT KG+GN Sbjct: 119 VVLGFIQALGMSVGFNNFF-PGLIPNPSVSVYLFIALVLTAGTAFLMWLGEQITAKGVGN 177 Query: 180 GISILIFAGIVAAIPGYITTTAQSSFIQP--DQXXXXXXXXXXXXXXXXXXXXXXXXXQQ 237 GISI+IFAGI A IP + S+ IQ +Q QQ Sbjct: 178 GISIIIFAGIAAGIPNGLNLI-YSTRIQDAGEQLFLNIVVILLLALAILAIIVGVIFVQQ 236 Query: 238 GIRKIPVQYAKRVVGNKMYGGQNTHIPLKINAAGVIPVIFAVSLLQFPIVISS-FWSTHE 296 +RKIPVQYAKR+VG GGQ+TH+PLK+NAAGVIPVIFA+SLL FP ++ F S H Sbjct: 237 ALRKIPVQYAKRLVGRNPVGGQSTHLPLKVNAAGVIPVIFALSLLIFPPTVAGLFGSDHP 296 Query: 297 WAKWVTNNLAHDKPLGMVLYVIMIIGFTFFYTFVQMNPQQMADNMKKNGGYIPGIRPGKA 356 A WV + +GM +Y + IIGFT+FY F+Q+NP++MA+N+KK GGYIPGIRPGKA Sbjct: 297 VAAWVIETFDYTHLIGMAVYALRIIGFTYFYAFIQVNPERMAENLKKQGGYIPGIRPGKA 356 Query: 357 TEKYLTRVMSRLTMSGALFLAVISILPVLFGSLSGLPRQVQIGGTALLIVVGVALDTMKQ 416 T+ Y+T ++ RLT G+LFLAV++ILPV F + LP+ +QIGGT LLIVVGVALDTMKQ Sbjct: 357 TQTYITPILYRLTFVGSLFLAVVAILPVFFIKFADLPQAIQIGGTGLLIVVGVALDTMKQ 416 Query: 417 IESQLIKRHYKGFIN 431 IE+QLIKR YKGFI Sbjct: 417 IEAQLIKRSYKGFIK 431 >ref|NP_691060.1| preprotein translocase subunit [Oceanobacillus iheyensis HTE831] dbj|BAC12095.1| preprotein translocase subunit [Oceanobacillus iheyensis HTE831] Length = 430 Score = 495 bits (1275), Expect = e-139, Method: Compositional matrix adjust. Identities = 239/431 (55%), Positives = 314/431 (72%), Gaps = 3/431 (0%) Query: 1 MFKTLKNIWHIEDLRKKILFTLFVLIIYRIGSFVPVPGVNKEVLESTNQGGEALMGLLNT 60 MF+TL N + D+R KI+FTL +LI++RIG+F+PVP +K ++ N+ + + G LNT Sbjct: 1 MFRTLSNFMRVADIRNKIIFTLLMLIVFRIGTFIPVPFTDKAAIDFMNE--QNVFGFLNT 58 Query: 61 FSGGALKNFSIFAISIYPYITASIIVQLLSMDVVPKFAEWAKQGEHGKKQLAQITRYGTV 120 F GGAL+NFSIFA+ I PYITASII+QLL MDVVPKFAEW KQGE G+K++AQ+TRYGT+ Sbjct: 59 FGGGALQNFSIFAMGIMPYITASIIMQLLQMDVVPKFAEWKKQGEMGRKKIAQVTRYGTI 118 Query: 121 VLGLIQAFATSIGFNRIYGTEMIPNATFADYLLIAIILTAGTSFLMWLGEQITEKGIGNG 180 VL +QA A S+GFN + G +I + +L IAI+LT GT+FLMW+GEQIT G+GNG Sbjct: 119 VLAFVQAIAMSVGFNAMAGGMLIADPNVMKFLTIAIVLTGGTAFLMWMGEQITAHGVGNG 178 Query: 181 ISILIFAGIVAAIPGYITTTAQSSFIQP-DQXXXXXXXXXXXXXXXXXXXXXXXXXQQGI 239 ISI+IFAGIVAA+P + F P + Q + Sbjct: 179 ISIIIFAGIVAAVPNGVGQLYSQYFSNPGNDLFINIVIVALIALVAVAVTVGVIFVNQAL 238 Query: 240 RKIPVQYAKRVVGNKMYGGQNTHIPLKINAAGVIPVIFAVSLLQFPIVISSFWSTHEWAK 299 RKIPVQYAK++V GGQ+TH+P+K+NAAGVIPVIFA++ + P I+ F+ ++ A Sbjct: 239 RKIPVQYAKKLVNRSPVGGQSTHLPIKVNAAGVIPVIFAIAFMMAPTTIAGFFEGNQVAD 298 Query: 300 WVTNNLAHDKPLGMVLYVIMIIGFTFFYTFVQMNPQQMADNMKKNGGYIPGIRPGKATEK 359 ++T + P+GM++YV MII FT+FYTFVQ+NP+QM++N++K GGYIPGIRPG TE+ Sbjct: 299 FITRTFDYTSPVGMIIYVAMIIAFTYFYTFVQVNPEQMSENLQKQGGYIPGIRPGHNTEE 358 Query: 360 YLTRVMSRLTMSGALFLAVISILPVLFGSLSGLPRQVQIGGTALLIVVGVALDTMKQIES 419 YLTRV++RLT GALFLAV+S+ P++ GSL+ LP VQIGGT+LLIVVGVAL TMKQ+E Sbjct: 359 YLTRVINRLTFVGALFLAVVSVFPMILGSLANLPSAVQIGGTSLLIVVGVALQTMKQLEG 418 Query: 420 QLIKRHYKGFI 430 QL+KRHYKGFI Sbjct: 419 QLVKRHYKGFI 429 >ref|NP_388017.1| preprotein translocase subunit [Bacillus subtilis] sp|P16336|SECY_BACSU Preprotein translocase secY subunit pir||BWBSSY preprotein translocase secY - Bacillus subtilis emb|CAA35712.1| SecY protein (AA 1-431) [Bacillus subtilis] gb|AAB06819.1| integral membrane protein gb|AAB59118.1| secY gene product emb|CAB11912.1| preprotein translocase subunit [Bacillus subtilis subsp. subtilis str. 168] Length = 431 Score = 482 bits (1240), Expect = e-135, Method: Compositional matrix adjust. Identities = 239/433 (55%), Positives = 311/433 (71%), Gaps = 3/433 (0%) Query: 1 MFKTLKNIWHIEDLRKKILFTLFVLIIYRIGSFVPVPGVNKEVLESTNQGGEALMGLLNT 60 MFKT+ N + D+R KI+FTL +LI++RIG+F+PVP VN E L++ +Q G + LLNT Sbjct: 1 MFKTISNFMRVSDIRNKIIFTLLMLIVFRIGAFIPVPYVNAEALQAQSQMG--VFDLLNT 58 Query: 61 FSGGALKNFSIFAISIYPYITASIIVQLLSMDVVPKFAEWAKQGEHGKKQLAQITRYGTV 120 F GGAL FSIFA+ I PYITASII+QLL MDVVPKF EW+KQGE G+++LAQ TRY T+ Sbjct: 59 FGGGALYQFSIFAMGITPYITASIIIQLLQMDVVPKFTEWSKQGEVGRRKLAQFTRYFTI 118 Query: 121 VLGLIQAFATSIGFNRIYGTEMIPNATFADYLLIAIILTAGTSFLMWLGEQITEKGIGNG 180 VLG IQA S GFN + +I + + YL+IA++LT GT+FLMWLGEQIT G+GNG Sbjct: 119 VLGFIQALGMSYGFNNLANGMLIEKSGVSTYLIIALVLTGGTAFLMWLGEQITSHGVGNG 178 Query: 181 ISILIFAGIVAAIPGYITTTAQSSFI-QPDQXXXXXXXXXXXXXXXXXXXXXXXXXQQGI 239 ISI+IFAGIV++IP I ++ F+ DQ QQ + Sbjct: 179 ISIIIFAGIVSSIPKTIGQIYETQFVGSNDQLFIHIVKVALLVIAILAVIVGVIFIQQAV 238 Query: 240 RKIPVQYAKRVVGNKMYGGQNTHIPLKINAAGVIPVIFAVSLLQFPIVISSFWSTHEWAK 299 RKI +QYAK + GGQ+TH+PLK+N AGVIPVIFAV+ L P I+SF+ T++ K Sbjct: 239 RKIAIQYAKGTGRSPAGGGQSTHLPLKVNPAGVIPVIFAVAFLITPRTIASFFGTNDVTK 298 Query: 300 WVTNNLAHDKPLGMVLYVIMIIGFTFFYTFVQMNPQQMADNMKKNGGYIPGIRPGKATEK 359 W+ NN + P+GM +YV +II FT+FY FVQ+NP+QMADN+KK GGYIPG+RPGK T+ Sbjct: 299 WIQNNFDNTHPVGMAIYVALIIAFTYFYAFVQVNPEQMADNLKKQGGYIPGVRPGKMTQD 358 Query: 360 YLTRVMSRLTMSGALFLAVISILPVLFGSLSGLPRQVQIGGTALLIVVGVALDTMKQIES 419 +T ++ RLT G++FLAVISILP+ F +GLP+ QIGGT+LLIVVGVAL+TMKQ+ES Sbjct: 359 RITSILYRLTFVGSIFLAVISILPIFFIQFAGLPQSAQIGGTSLLIVVGVALETMKQLES 418 Query: 420 QLIKRHYKGFINK 432 QL+KR+Y+GF+ Sbjct: 419 QLVKRNYRGFMKN 431 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.141 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 589,844,273 Number of extensions: 23102581 Number of successful extensions: 80808 Number of sequences better than 1.0e-05: 968 Number of HSP's gapped: 78029 Number of HSP's successfully gapped: 1214 Length of query: 432 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 303 Effective length of database: 406,989,807 Effective search space: 123317911521 Effective search space used: 123317911521 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)