BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0026.p (388 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAD40109.1| putative acyl-CoA dehydrogenase [Bacillus cereus] 361 2e-98 ref|ZP_00110259.1| COG1960: Acyl-CoA dehydrogenases [Nostoc punc... 325 1e-87 gb|AAL32268.1| putative acyl-CoA dehydrogenase [Bacillus thuring... 294 2e-78 gb|AAR87758.1| acyl-CoA dehydrogenase [Bacillus cereus] 247 3e-64 ref|NP_563216.1| acyl-CoA dehydrogenase [Clostridium perfringens... 233 8e-60 >gb|AAD40109.1| putative acyl-CoA dehydrogenase [Bacillus cereus] Length = 382 Score = 361 bits (926), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 182/369 (49%), Positives = 258/369 (69%), Gaps = 2/369 (0%) Query: 6 NNSQSILEEVKQFVEEDIRPFAADFEEQQALPRELIRKMGRKGYLGASFPEEFGGLELDP 65 +N++ IL V +F E+ +RP A++FE + LP ++I+++ G LGA+ P+E+GGL LD Sbjct: 2 SNAEEILGVVDEFAEKKLRPRASEFESNEELPYDVIQEISSYGVLGATIPKEYGGLSLDS 61 Query: 66 LEYGSLVEEIGKGALSAAMLLTVSTGLVGETLIRWGTKYQQQKYVLPIARGEKIGAFALT 125 L+YG L E IGK S LLTV LVGE++ RWGT+ Q++K++ +A+G + +FALT Sbjct: 62 LDYGRLTEIIGKACNSVRELLTVHVSLVGESIKRWGTEEQKRKWLPEMAKGNLLFSFALT 121 Query: 126 EPGVGSGAKDVESAYEKVGDNYRITGRKKWISFGEIADFFIVIAAEPSSKAVSAFLVDRN 185 EP VGS AK V ++Y++V D++ + G KKWISF +IAD F+V A+ S VSAF+V+R+ Sbjct: 122 EPEVGSDAKAVGTSYKQVNDHFILNGHKKWISFADIADCFLVFAS--SEGKVSAFIVERS 179 Query: 186 LEGVKIKKMQGLLSCAASQLADIELTGVEVGPEMMVGKEGMGFAGIANFALDYGRFNVAW 245 + GV KM LL+ +S +A+I L V+V E ++G G G++ + N ALD+GR+++AW Sbjct: 180 MTGVSTNKMSKLLASNSSHIAEIHLQDVKVPAENLLGPIGGGWSYVVNTALDHGRYSIAW 239 Query: 246 MGVGLAQEALEVMVTYARKRSQSGQKIFHHQLIKGIIGDNVTNIMAARSLCKEAARMRIE 305 GV +AQEALE MV Y+R+R Q + I ++ I+ I+ + NI AARSLC+EA + R + Sbjct: 240 AGVAIAQEALEAMVAYSRRRKQGNKYICEYEAIQTILAEASVNIKAARSLCQEAGKKRQD 299 Query: 306 NHPDAAIHTTAAKYYSSTVANKAAADAIQVLGANGCSRRFPVERLFREAKVLEIIEGTSQ 365 DA I TT AKY++S +A K A DA+QV G NG SR +PVERLFREAKVLEIIEGTSQ Sbjct: 300 RQTDAVIETTIAKYFASKMAMKVATDAVQVFGGNGFSREYPVERLFREAKVLEIIEGTSQ 359 Query: 366 LQQQIIALY 374 + Q IIA + Sbjct: 360 VLQPIIAQH 368 >ref|ZP_00110259.1| COG1960: Acyl-CoA dehydrogenases [Nostoc punctiforme] Length = 395 Score = 325 bits (832), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 163/370 (44%), Positives = 237/370 (64%), Gaps = 3/370 (0%) Query: 14 EVKQFVEEDIRPFAADFEEQQALPRELIRKMGRKGYLGASFPEEFGGLELDPLEYGSLVE 73 E ++FV+ ++ A +++Q+ P +L++K +K YLGA P+EFGG +D + YG L E Sbjct: 16 EFREFVDAEVVSQANQYDQQEYTPSDLVQKFAQKRYLGAVLPKEFGGEAMDMITYGLLNE 75 Query: 74 EIGKGALSAAMLLTVSTGLVGETLIRWGTKYQQQKYVLPIARGEKIGAFALTEPGVGSGA 133 EIG+G S LLTV +V L +WG K Q+ ++ +A GE I AFAL+EP VGS A Sbjct: 76 EIGRGCSSLRSLLTVHC-MVSHALCKWGNKSQKNDWLPKLASGEIIAAFALSEPNVGSDA 134 Query: 134 KDVESAYEKVGDNYRITGRKKWISFGEIADFFIVIAAEPSSKAVSAFLVDRNLEGVKIKK 193 K VE+ GD Y + G KKWI++G+IAD F+V A SAFLV++N G+ IK Sbjct: 135 KSVETTATLSGDAYILNGHKKWITYGQIADVFLVFAQ--CEGKPSAFLVEKNSPGLSIKP 192 Query: 194 MQGLLSCAASQLADIELTGVEVGPEMMVGKEGMGFAGIANFALDYGRFNVAWMGVGLAQE 253 + G+L AS LA++ L + E +VG+ G GF+ +A+ ALDYGR++VAW VG+AQ Sbjct: 193 IFGMLGVRASMLAELHLNDCRIPQENLVGRLGFGFSYVASSALDYGRYSVAWGSVGIAQA 252 Query: 254 ALEVMVTYARKRSQSGQKIFHHQLIKGIIGDNVTNIMAARSLCKEAARMRIENHPDAAIH 313 L + Y R Q G + HQLI+ +I + + NI AAR LC +A ++ P + I Sbjct: 253 CLSACIRYTSDRKQFGVYLKEHQLIRQMITNMIANIKAARLLCYQAGYLKEIGDPSSIIE 312 Query: 314 TTAAKYYSSTVANKAAADAIQVLGANGCSRRFPVERLFREAKVLEIIEGTSQLQQQIIAL 373 T+ AKY++ST+A++ A+DA+Q+ G NGCS +PVER R+AK++EIIEG++Q+QQ IA Sbjct: 313 TSIAKYFTSTIASQVASDAVQIHGGNGCSSEYPVERYLRDAKIMEIIEGSTQIQQVTIAE 372 Query: 374 YGANKYYQPK 383 YG +Y P+ Sbjct: 373 YGYQEYLSPR 382 >gb|AAL32268.1| putative acyl-CoA dehydrogenase [Bacillus thuringiensis serovar kurstaki] Length = 309 Score = 294 bits (753), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 149/297 (50%), Positives = 209/297 (70%), Gaps = 2/297 (0%) Query: 85 LLTVSTGLVGETLIRWGTKYQQQKYVLPIARGEKIGAFALTEPGVGSGAKDVESAYEKVG 144 LLTV LVGE++ RWGT+ Q++K++ +A+G + +FALTEP VGS AK V ++Y++V Sbjct: 8 LLTVHESLVGESIKRWGTEEQKRKWLPEMAKGNLLFSFALTEPEVGSDAKAVGTSYKQVN 67 Query: 145 DNYRITGRKKWISFGEIADFFIVIAAEPSSKAVSAFLVDRNLEGVKIKKMQGLLSCAASQ 204 D++ + G KKWISF +IAD F+V A+ S VSAF+V+R++ GV KM LL+ +S Sbjct: 68 DHFILNGHKKWISFADIADCFLVFAS--SEGKVSAFIVERSMTGVSTNKMSKLLASNSSH 125 Query: 205 LADIELTGVEVGPEMMVGKEGMGFAGIANFALDYGRFNVAWMGVGLAQEALEVMVTYARK 264 +A+I L V+V E ++G G G++ + N ALD+GR+++AW GV +AQEALE MV Y+R+ Sbjct: 126 IAEIHLQDVKVPAENLLGPIGGGWSYVVNTALDHGRYSIAWAGVAIAQEALEAMVAYSRR 185 Query: 265 RSQSGQKIFHHQLIKGIIGDNVTNIMAARSLCKEAARMRIENHPDAAIHTTAAKYYSSTV 324 R Q + I ++ I+ I+ + NI AARSLC+EA + R + H DA I TT AKY++S + Sbjct: 186 RKQGNKYICEYEAIQTILAEASVNIKAARSLCQEAGKKRQDRHTDAVIETTIAKYFASKM 245 Query: 325 ANKAAADAIQVLGANGCSRRFPVERLFREAKVLEIIEGTSQLQQQIIALYGANKYYQ 381 A K A +A+QV G NG SR +PVERLFREAKVLEIIEGTSQ+ Q IIA + + Q Sbjct: 246 AMKVATNAVQVFGGNGFSREYPVERLFREAKVLEIIEGTSQVLQPIIAQHALHTCSQ 302 >gb|AAR87758.1| acyl-CoA dehydrogenase [Bacillus cereus] Length = 397 Score = 247 bits (631), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 121/355 (34%), Positives = 212/355 (59%), Gaps = 2/355 (0%) Query: 17 QFVEEDIRPFAADFEEQQALPRELIRKMGRKGYLGASFPEEFGGLELDPLEYGSLVEEIG 76 FV E+I P A +E +Q +P ++I+ + GYLG + P E+GGLE D + YG E I Sbjct: 20 DFVCENIEPHANRWEIEQGIPSDIIKTCAKNGYLGGTLPAEYGGLEWDYVTYGLFTEAIA 79 Query: 77 KGALSAAMLLTVSTGLVGETLIRWGTKYQQQKYVLPIARGEKIGAFALTEPGVGSGAKDV 136 +G++S + L V T +V ET+++WGT+ Q+ +++ +A G +I A ALTEPG GS + Sbjct: 80 RGSVSLSGLFNVHT-MVTETILKWGTENQKNQWLPLLASGNQIAALALTEPGAGSDLNMI 138 Query: 137 ESAYEKVGDNYRITGRKKWISFGEIADFFIVIAAEPSSKAVSAFLVDRNLEGVKIKKMQG 196 ++ +++ GD + G KKWI+FG AD F+V + + A LV ++ GV + ++ Sbjct: 139 KTNFDQHGDKLILNGVKKWITFGAAADVFLVFGKLNGEQPI-ACLVPKDSVGVNVTHIRD 197 Query: 197 LLSCAASQLADIELTGVEVGPEMMVGKEGMGFAGIANFALDYGRFNVAWMGVGLAQEALE 256 +L A+ LA+IE VE+ E ++G+ G F +A +AL++GR ++A+ +G+ + LE Sbjct: 198 MLGFKAAYLAEIEFNNVEIPFENIIGRPGFAFTYLAPYALEFGRISIAFAALGVLRTCLE 257 Query: 257 VMVTYARKRSQSGQKIFHHQLIKGIIGDNVTNIMAARSLCKEAARMRIENHPDAAIHTTA 316 + + RS G + H +I ++ D ++ A+ L EA + + E+ DA Sbjct: 258 ICGNHVLHRSAFGNSLIDHGMISHMVTDMGVDLEASILLSLEACKAKNEHRADATEKIMI 317 Query: 317 AKYYSSTVANKAAADAIQVLGANGCSRRFPVERLFREAKVLEIIEGTSQLQQQII 371 AKY++S + + +A+Q++GA GC+ + + R FR++K +EI+EG++Q+ Q ++ Sbjct: 318 AKYFASRSGTRHSTNAVQIMGALGCNDNYSISRCFRDSKTMEIVEGSNQIHQLLL 372 >ref|NP_563216.1| acyl-CoA dehydrogenase [Clostridium perfringens] dbj|BAB82006.1| acyl-CoA dehydrogenase [Clostridium perfringens str. 13] Length = 379 Score = 233 bits (593), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 129/361 (35%), Positives = 214/361 (59%), Gaps = 5/361 (1%) Query: 15 VKQFVEEDIRPFAADFEEQQALPRELIRKMGRKGYLGASFPEEFGGLELDPLEYGSLVEE 74 V++F + +++P AA+ + + P E ++KM + G +G F +E+GG D L Y VEE Sbjct: 16 VREFAQNEVKPIAAEIDVTERFPMENVKKMAKLGMMGIPFSKEYGGAGGDTLSYILTVEE 75 Query: 75 IGKGALSAAMLLTVSTGLVGETLIRWGTKYQQQKYVLPIARGEKIGAFALTEPGVGSGAK 134 + K + ++++ T L + ++GT Q++KY+ P+A+GEK+GAF LTEPG G+ A Sbjct: 76 LSKVCATTGVIVSAHTSLCASLIDQFGTPAQKEKYLTPLAKGEKLGAFGLTEPGAGTDAA 135 Query: 135 DVESAYEKVGDNYRITGRKKWISFGEIADFFIVIAAEPSS---KAVSAFLVDRNLEGVKI 191 ++ GDNY + G K +I+ G +AD FI+ A S + +SAF+V++N G I Sbjct: 136 GQQTTAVLEGDNYILNGSKIFITNGGVADTFIIFAMTDKSQGTRGISAFIVEKNFPGFSI 195 Query: 192 KKMQGLLSCAASQLADIELTGVEVGPEMMVGKEGMGFAGIANFALDYGRFNVAWMGVGLA 251 K++ + AS ++ + V E ++G+EG GF GIA LD GR +A +G+A Sbjct: 196 GKLEEKMGIRASSTTELIMENCVVPKENLIGREGKGF-GIAMKTLDGGRIGIAAQALGIA 254 Query: 252 QEALEVMVTYARKRSQSGQKIFHHQLIKGIIGDNVTNIMAARSLCKEAARMRIENHPDAA 311 + ALE V Y ++R Q G+ + Q ++ ++ + T I AAR L +AA ++ E P + Sbjct: 255 EGALEEAVAYMKERKQFGRSLSAFQGLQWMVAEMDTKIEAARFLVYKAACLKEEGKP-YS 313 Query: 312 IHTTAAKYYSSTVANKAAADAIQVLGANGCSRRFPVERLFREAKVLEIIEGTSQLQQQII 371 I AK +++ VA + A+Q+ G G ++ +PVER+ R+AK+ EI EGTS++Q+ +I Sbjct: 314 IDAARAKLFAADVAMEVTTKAVQLFGGYGYTKEYPVERMMRDAKITEIYEGTSEVQKMVI 373 Query: 372 A 372 A Sbjct: 374 A 374 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 534,906,128 Number of extensions: 21353280 Number of successful extensions: 61886 Number of sequences better than 1.0e-05: 2757 Number of HSP's gapped: 57738 Number of HSP's successfully gapped: 2763 Length of query: 388 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 261 Effective length of database: 412,420,005 Effective search space: 107641621305 Effective search space used: 107641621305 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)