BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0023.p (344 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00110260.1| COG3882: Predicted enzyme involved in methoxy... 379 e-104 gb|AAF86387.1| FkbH [Streptomyces hygroscopicus var. ascomyceticus] 259 6e-68 gb|AAO06922.1| GdmH [Streptomyces hygroscopicus] 256 7e-67 gb|AAM54094.1| unknown [Actinosynnema pretiosum subsp. auranticum] 221 1e-56 ref|ZP_00060858.1| COG3882: Predicted enzyme involved in methoxy... 130 5e-29 >ref|ZP_00110260.1| COG3882: Predicted enzyme involved in methoxymalonyl-ACP biosynthesis [Nostoc punctiforme] Length = 356 Score = 379 bits (974), Expect = e-104, Method: Compositional matrix adjust. Identities = 188/337 (55%), Positives = 232/337 (68%), Gaps = 1/337 (0%) Query: 7 IKCVVWDLDNTLWDGVLSEGDEVTLKPQIKEILEELDHRGILLSVASKNNLDDAMNKLKE 66 IKCVVWDLDNTLW GVL E + V L+ I I++ LD RGIL SVASKN+ AMNKL+E Sbjct: 19 IKCVVWDLDNTLWHGVLLEDNHVYLRDDIVNIIKTLDSRGILQSVASKNDCVLAMNKLQE 78 Query: 67 FEIDHYFLYPQIHWEPKSKSLDAIRQSINIGMDTLLFIDDRPFELDEVKSVHAAINCVHA 126 F + YFLYPQI+W K+ S+ I + INIG D + FIDD+PFEL+EV + C++ Sbjct: 79 FGLQEYFLYPQINWNSKAASIQEIAKLINIGTDAIAFIDDQPFELEEVNFSLPEVFCINV 138 Query: 127 DHYQELLDHPSLIPAFITEDSVKRRLMYLEDMKRKQEEEAFSGTSEEFLKSLGMKFIISX 186 LLD P + P FITEDS RRLMY+ D+KR+ E+ F GT EEFL +L M F IS Sbjct: 139 SQLAHLLDMPEMNPRFITEDSKIRRLMYISDIKRQNTEKEFVGTPEEFLATLKMSFTISS 198 Query: 187 XXXXXXXXXXXXTVRTNQLNSTGRIYSYDELNHFRVSPGHKLFVCELTDKYGSYGKIGIA 246 TVRTNQLN+TG YSYDELNHFR S HKLF+ L DKYGSYGKIG+ Sbjct: 199 AKEEDLQRAEELTVRTNQLNTTGYTYSYDELNHFRQSESHKLFIASLDDKYGSYGKIGLV 258 Query: 247 LAELTDTHWHIKLLIMSCRVISHGVGTVLLTHIMQQAKQANKRLLADFKNTERNKMMYVS 306 L E ++ W IKLL+MSCRV+S G+GT++L HIM AK N RL A+F ++ RN+MMY++ Sbjct: 259 LIECQESIWTIKLLLMSCRVMSRGIGTIMLNHIMNLAKNNNVRLCAEFVSSHRNRMMYIA 318 Query: 307 YRFSNFKEIAKAEDGFVILENDLTTIQKFPPYIELLV 343 Y+F+ FKEI K + VI ENDLT IQ FP YI+L + Sbjct: 319 YKFAGFKEIEKRGE-LVIFENDLTRIQPFPKYIKLQI 354 >gb|AAF86387.1| FkbH [Streptomyces hygroscopicus var. ascomyceticus] Length = 360 Score = 259 bits (663), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 126/308 (40%), Positives = 179/308 (58%) Query: 7 IKCVVWDLDNTLWDGVLSEGDEVTLKPQIKEILEELDHRGILLSVASKNNLDDAMNKLKE 66 +KC+VWDLDNTLW G + E DEV L +I+E++ LD RGIL +VASKN+ D A +L+ Sbjct: 4 VKCLVWDLDNTLWRGTVLEDDEVVLTDEIREVITTLDDRGILQAVASKNDHDLAWERLER 63 Query: 67 FEIDHYFLYPQIHWEPKSKSLDAIRQSINIGMDTLLFIDDRPFELDEVKSVHAAINCVHA 126 + YF+ +I W PKS+S+ I +N T+ FIDD+P E EV + C A Sbjct: 64 LGVAEYFVLARIGWGPKSQSVREIATELNFAPTTIAFIDDQPAERAEVAFHLPEVRCYPA 123 Query: 127 DHYQELLDHPSLIPAFITEDSVKRRLMYLEDMKRKQEEEAFSGTSEEFLKSLGMKFIISX 186 + LL P P T DS +RRLMY R Q EA+SG E+FL+SL + I+ Sbjct: 124 EQAATLLSLPEFSPPVSTVDSRRRRLMYQAGFARDQAREAYSGPDEDFLRSLDLSMTIAP 183 Query: 187 XXXXXXXXXXXXTVRTNQLNSTGRIYSYDELNHFRVSPGHKLFVCELTDKYGSYGKIGIA 246 T+RT+Q+N+TG YS +L P H++ V + D++G +G +GI Sbjct: 184 AGEEELSRVEELTLRTSQMNATGVHYSDADLRACFTDPAHEVLVVTMGDRFGPHGAVGII 243 Query: 247 LAELTDTHWHIKLLIMSCRVISHGVGTVLLTHIMQQAKQANKRLLADFKNTERNKMMYVS 306 L E + WH+KLL SCRV+S G G +L + Q +A L+ADF+ T+RN+MM ++ Sbjct: 244 LLEKKPSTWHLKLLATSCRVVSFGAGATILNWLTDQGARAGAHLVADFRRTDRNRMMEIA 303 Query: 307 YRFSNFKE 314 YRF+ F + Sbjct: 304 YRFAGFAD 311 >gb|AAO06922.1| GdmH [Streptomyces hygroscopicus] Length = 370 Score = 256 bits (653), Expect = 7e-67, Method: Compositional matrix adjust. Identities = 127/317 (40%), Positives = 177/317 (55%) Query: 2 EIKNGIKCVVWDLDNTLWDGVLSEGDEVTLKPQIKEILEELDHRGILLSVASKNNLDDAM 61 E +KC+VWDLDNTLW G L E EV +++++ LD RGIL S+ASKN+ D A Sbjct: 9 EPPTAVKCLVWDLDNTLWRGTLLEDGEVLPFEWVRDVITTLDERGILQSIASKNDHDHAW 68 Query: 62 NKLKEFEIDHYFLYPQIHWEPKSKSLDAIRQSINIGMDTLLFIDDRPFELDEVKSVHAAI 121 +L+ + YF+ P I W PKS SL AI +N + F+DD+P E EV + Sbjct: 69 ERLEALGLAEYFVLPHIGWGPKSASLRAIADRLNFADRAMAFVDDQPAERAEVTYRLPEV 128 Query: 122 NCVHADHYQELLDHPSLIPAFITEDSVKRRLMYLEDMKRKQEEEAFSGTSEEFLKSLGMK 181 C A+ L P PA +T DS +RR MY +R E FSG E+FL++L ++ Sbjct: 129 RCYAAEDLAGLTRLPEFSPAVVTVDSRQRRNMYQSGFRRDAERAEFSGPDEDFLRTLDIR 188 Query: 182 FIISXXXXXXXXXXXXXTVRTNQLNSTGRIYSYDELNHFRVSPGHKLFVCELTDKYGSYG 241 IS T+RT+Q+N+TG Y L P H++ V + D++G +G Sbjct: 189 MGISRATERELSRVEELTLRTSQMNATGVHYPDSALRGLLTDPAHEVLVITMADRFGPHG 248 Query: 242 KIGIALAELTDTHWHIKLLIMSCRVISHGVGTVLLTHIMQQAKQANKRLLADFKNTERNK 301 +GI L E WH+KLL SCRV+S+G G+ LL+ + QA QA L ADF+ T+RN+ Sbjct: 249 AVGIVLLERHPAVWHLKLLATSCRVVSYGAGSTLLSWLADQAAQAGVHLAADFRRTDRNR 308 Query: 302 MMYVSYRFSNFKEIAKA 318 MM V+YRF+ F + A Sbjct: 309 MMEVAYRFAGFADTTCA 325 >gb|AAM54094.1| unknown [Actinosynnema pretiosum subsp. auranticum] Length = 437 Score = 221 bits (564), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 107/279 (38%), Positives = 158/279 (56%) Query: 34 QIKEILEELDHRGILLSVASKNNLDDAMNKLKEFEIDHYFLYPQIHWEPKSKSLDAIRQS 93 +++ + ELD RG+L SVAS+N+ + A+ L+ + YF+ P+I W KS ++ I Sbjct: 5 RVRAAIVELDARGVLQSVASRNDPEPALAALEALGVAEYFVLPEIGWGVKSAAVARIADR 64 Query: 94 INIGMDTLLFIDDRPFELDEVKSVHAAINCVHADHYQELLDHPSLIPAFITEDSVKRRLM 153 + + F+DDRP E EV + C A+ EL P PA +TED+ RR + Sbjct: 65 LGFAHRAIAFVDDRPAERAEVAFHLPEVRCYPAERVLELCSLPEFSPAVVTEDARNRRAL 124 Query: 154 YLEDMKRKQEEEAFSGTSEEFLKSLGMKFIISXXXXXXXXXXXXXTVRTNQLNSTGRIYS 213 Y R E+ AF+G EEFL+SLG+ I T RT+Q+N+TG Y Sbjct: 125 YRAGFARGAEQAAFTGPDEEFLRSLGLVLRIDRARPEDLHRVEELTARTSQMNATGVHYP 184 Query: 214 YDELNHFRVSPGHKLFVCELTDKYGSYGKIGIALAELTDTHWHIKLLIMSCRVISHGVGT 273 + +L P H++ V LTD++G +G IGI L E WH+KLL SCRV+ +GVG+ Sbjct: 185 HGQLRALLDDPAHEVLVAGLTDRFGPHGAIGIVLLERHTAAWHLKLLATSCRVVPYGVGS 244 Query: 274 VLLTHIMQQAKQANKRLLADFKNTERNKMMYVSYRFSNF 312 VLL + QA +A L+ADF+ T+RN+MM ++YRF+ + Sbjct: 245 VLLDWLCGQALEAGAHLVADFRRTDRNRMMEIAYRFAGY 283 >ref|ZP_00060858.1| COG3882: Predicted enzyme involved in methoxymalonyl-ACP biosynthesis [Clostridium thermocellum ATCC 27405] Length = 551 Score = 130 bits (326), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 97/319 (30%), Positives = 164/319 (51%), Gaps = 30/319 (9%) Query: 8 KCVVWDLDNTLWDGVLSEG----------DEVTLKPQIKEILEELDHRGILLSVASKNNL 57 KC+V DLDNTLW GV+ E E + L+E+ GI+L+V SKNN Sbjct: 191 KCLVLDLDNTLWGGVVGEAGIEGIELSDYKEGARYKDFQRKLKEIKDLGIILAVVSKNNF 250 Query: 58 DDAMNKLKEFE----IDHYFLYPQIHWEPKSKSLDAIRQSINIGMDTLLFIDDRPFELDE 113 DDA+ ++E + + F+ +I+W+ KS+++ + + +NIG+D+++FIDD P E + Sbjct: 251 DDAIKVIREHKHMVLKEEDFVALKINWDLKSQNIRDLSEELNIGLDSMVFIDDNPVERES 310 Query: 114 VKSVHAAINCVHADHYQ---ELLDHPSLI--PAFIT-----EDSVKRRLMYLEDMKRKQE 163 VK + V D Q EL+D + + F T ED+VK + Y ++MKR+ Sbjct: 311 VKRELPEV--VVPDFPQDSSELVDFATELYNNYFYTLDTTYEDTVKTEI-YRQNMKRRDA 367 Query: 164 EEAFSGTSEEFLKSLGMKFIISXXXXXXXXXXXXXTVRTNQLNSTGRIYSYDELNHFRVS 223 +++ S + E+FL+SL + I T +TNQ N T + YS EL Sbjct: 368 QKS-SASYEDFLRSLETRIEIRRINAENVQRAAQLTQKTNQFNLTTKRYSEQELLALIND 426 Query: 224 PGHKLFVCELTDKYGSYGKIGIALAEL-TDTHWHIKLLIMSCRVISHGVGTVLLTHIMQQ 282 G + FV ++DK+G G + + + +D+ + ++SCRV+ + ++ I Sbjct: 427 QGFEGFVAYVSDKFGDNGMVSVVITRRKSDSEVELDTFLLSCRVMGRFIEDRIIGFIEDL 486 Query: 283 AKQAN-KRLLADFKNTERN 300 K+A K+ + ++ TE+N Sbjct: 487 YKKAGYKKFITYYRPTEKN 505 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 484,810,143 Number of extensions: 18968473 Number of successful extensions: 57501 Number of sequences better than 1.0e-05: 60 Number of HSP's gapped: 57397 Number of HSP's successfully gapped: 60 Length of query: 344 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 218 Effective length of database: 415,135,104 Effective search space: 90499452672 Effective search space used: 90499452672 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 126 (53.1 bits)