BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0022.p (293 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAR87756.1| putative 3-hydroxybutyryl-CoA dehydrogenase [Baci... 292 5e-78 gb|AAL32270.1| 3-hydroxybutyryl-CoA dehydrogenase protein [Bacil... 291 1e-77 ref|ZP_00110257.1| COG1250: 3-hydroxyacyl-CoA dehydrogenase [Nos... 271 2e-71 gb|AAK19885.1| beta-hydroxybutyryl-CoA dehydrogenase [Polyangium... 270 3e-71 ref|NP_754359.1| Putative 3-hydroxyacyl-CoA dehydrogenase [Esche... 244 2e-63 >gb|AAR87756.1| putative 3-hydroxybutyryl-CoA dehydrogenase [Bacillus cereus] Length = 282 Score = 292 bits (748), Expect = 5e-78, Method: Compositional matrix adjust. Identities = 141/281 (50%), Positives = 186/281 (66%) Query: 4 FETIGIIGAGVMGSDLALDLASQGYRVILKDIHEEALEKAKVKIREDFKLVKLMKKELPV 63 F+ IGI+GAG MG +A+DL G +L D+ EE LEKA+ KI E + L+ K P Sbjct: 2 FKKIGIVGAGTMGIGMAVDLVLHGLETVLIDVTEEQLEKAEEKILETVRFAPLINKAFPR 61 Query: 64 KKIEQVLSNIMFTTTYQSFHAVPFVIENITENWEAKQKVYLELQEHCQEDTIYGVNTSCI 123 E+VLS I +T +++EN+ ENW+ K+ +Y L E C++DTI+GVNTSCI Sbjct: 62 MHKEEVLSLISSSTNLDEVAECDYIVENVPENWQIKEPIYRRLDEICKKDTIFGVNTSCI 121 Query: 124 SITKVGAILPRPEHAIGMHFMNPVPLKQMVEIIKGYYTSEDTIRKIKDFLKAIDKKPVLV 183 SITKVG + RP+ IGMHFMNPV +K +E+I+G+ TS++T+ K + FLK +DK ++V Sbjct: 122 SITKVGGVTKRPDKIIGMHFMNPVYMKPSIEVIRGHLTSDETVEKAQSFLKQLDKDAIVV 181 Query: 184 NDSPGFVTNRVLMPMINECIWAVHDGVAAPQDVDKIFRLGFSHKMGPLATADLIGLDTIL 243 ND GFV+NR+ +NE W V DGVA P+ VD IF+ F H MGPL TADLIGLDT+L Sbjct: 182 NDQTGFVSNRISHLFMNESAWVVMDGVATPKQVDDIFKKCFGHTMGPLETADLIGLDTVL 241 Query: 244 NSILVLYESYNDPKYRPCPLLVKMVDAXXXXXXXXXXFFDY 284 +S+ VLYE Y DPK+R CPLL KMVDA F+ Y Sbjct: 242 HSLNVLYEEYQDPKFRCCPLLKKMVDAGECGRKSGKGFYAY 282 >gb|AAL32270.1| 3-hydroxybutyryl-CoA dehydrogenase protein [Bacillus thuringiensis serovar kurstaki] Length = 282 Score = 291 bits (745), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 141/281 (50%), Positives = 185/281 (65%) Query: 4 FETIGIIGAGVMGSDLALDLASQGYRVILKDIHEEALEKAKVKIREDFKLVKLMKKELPV 63 F+ IGI+GAG MG +A+DL G +L D+ EE LE A+ KI E + L+ K P Sbjct: 2 FKKIGIVGAGTMGIGMAVDLVLHGLETVLIDVTEEQLEIAEEKILETVRFAPLINKAFPR 61 Query: 64 KKIEQVLSNIMFTTTYQSFHAVPFVIENITENWEAKQKVYLELQEHCQEDTIYGVNTSCI 123 E+VLS I +T +++EN+ ENW+ K+ +Y L E C++DTI+GVNTSCI Sbjct: 62 MHKEEVLSLISSSTNLDEVAECDYIVENVPENWQIKEPIYRRLDEICKKDTIFGVNTSCI 121 Query: 124 SITKVGAILPRPEHAIGMHFMNPVPLKQMVEIIKGYYTSEDTIRKIKDFLKAIDKKPVLV 183 SITKVG + RP+ IGMHFMNPV +K +E+I+GY TS++T+ K + FLK +DK ++V Sbjct: 122 SITKVGGVTKRPDKIIGMHFMNPVYMKPSIEVIRGYLTSDETVEKAQSFLKQLDKDAIVV 181 Query: 184 NDSPGFVTNRVLMPMINECIWAVHDGVAAPQDVDKIFRLGFSHKMGPLATADLIGLDTIL 243 ND GFV+NR+ +NE W V DGVA P+ VD IF+ F H MGPL TADLIGLDT+L Sbjct: 182 NDQTGFVSNRISHLFMNESAWVVMDGVATPKQVDDIFKKCFGHTMGPLETADLIGLDTVL 241 Query: 244 NSILVLYESYNDPKYRPCPLLVKMVDAXXXXXXXXXXFFDY 284 +S+ VLYE Y DPK+R CPLL KMVDA F+ Y Sbjct: 242 HSLNVLYEEYQDPKFRCCPLLKKMVDAGECGRKSGKGFYAY 282 >ref|ZP_00110257.1| COG1250: 3-hydroxyacyl-CoA dehydrogenase [Nostoc punctiforme] Length = 288 Score = 271 bits (692), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 130/265 (49%), Positives = 179/265 (67%) Query: 22 DLASQGYRVILKDIHEEALEKAKVKIREDFKLVKLMKKELPVKKIEQVLSNIMFTTTYQS 81 +LA G++VIL D+ E+ L AK +IR + + +K + + +L+ I F+T Y+ Sbjct: 22 NLAQTGHQVILVDVSEDILNHAKQEIRNNVRFQGFFQKNSEAESPDDILNKIKFSTNYKF 81 Query: 82 FHAVPFVIENITENWEAKQKVYLELQEHCQEDTIYGVNTSCISITKVGAILPRPEHAIGM 141 FV+EN+TE W+ K++VY +L C E T + NTS ISIT++ ++ R +GM Sbjct: 82 LEDAEFVVENVTEKWDLKKEVYAQLDAICPEKTAFAANTSAISITRIASVTKRATRVVGM 141 Query: 142 HFMNPVPLKQMVEIIKGYYTSEDTIRKIKDFLKAIDKKPVLVNDSPGFVTNRVLMPMINE 201 HFMNPVP+K MVE+I+G++TS++TI K L + K+ ++VNDSPGFV+NRVLM INE Sbjct: 142 HFMNPVPMKPMVEMIRGHHTSDETIETAKKMLAQMGKECIIVNDSPGFVSNRVLMLTINE 201 Query: 202 CIWAVHDGVAAPQDVDKIFRLGFSHKMGPLATADLIGLDTILNSILVLYESYNDPKYRPC 261 I+ + D VA+ ++VDKIF+ F HKMGPL TADLIGLDTIL SI VLYES+ND KYRPC Sbjct: 202 AIFILQDQVASIEEVDKIFKTCFGHKMGPLETADLIGLDTILFSIEVLYESFNDSKYRPC 261 Query: 262 PLLVKMVDAXXXXXXXXXXFFDYTS 286 PLL KMVDA F+ Y++ Sbjct: 262 PLLKKMVDAGLHGRKSGKGFYAYSN 286 >gb|AAK19885.1| beta-hydroxybutyryl-CoA dehydrogenase [Polyangium cellulosum] Length = 293 Score = 270 bits (690), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 133/265 (50%), Positives = 174/265 (65%), Gaps = 3/265 (1%) Query: 23 LASQGYRVILKDIHEEALEKAKVKIREDFKLVKLMKKELPVKKI---EQVLSNIMFTTTY 79 LA G+ V+L D+ E AL +A++ IR + V L K+ + VL + FTT Y Sbjct: 28 LAQTGHDVVLVDVSEAALARARMGIRNGLRAVTLFGSAEDKKRAGDPKAVLERVAFTTDY 87 Query: 80 QSFHAVPFVIENITENWEAKQKVYLELQEHCQEDTIYGVNTSCISITKVGAILPRPEHAI 139 FV+EN+TE W+ K++VY L+ C+ + I+ +TS ISIT++G++ RP + Sbjct: 88 GRLAGADFVVENVTEKWDIKREVYARLEGVCRPEIIFAADTSAISITRIGSVTKRPSQVV 147 Query: 140 GMHFMNPVPLKQMVEIIKGYYTSEDTIRKIKDFLKAIDKKPVLVNDSPGFVTNRVLMPMI 199 GMHFMNPVPLK MVE+I+G++TS +T+ K FL + K V+V D+PGFV+NRVLM I Sbjct: 148 GMHFMNPVPLKPMVEVIRGFHTSPETLGAAKRFLAEMGKTCVVVEDAPGFVSNRVLMLTI 207 Query: 200 NECIWAVHDGVAAPQDVDKIFRLGFSHKMGPLATADLIGLDTILNSILVLYESYNDPKYR 259 NE I+ V D VA+ DVD+IF+ F HKMGPL TADLIGLDTIL SI VLYES+ND KYR Sbjct: 208 NEAIFLVQDQVASAADVDRIFKGCFEHKMGPLETADLIGLDTILYSIEVLYESFNDDKYR 267 Query: 260 PCPLLVKMVDAXXXXXXXXXXFFDY 284 PCPLL KMVDA F+ Y Sbjct: 268 PCPLLKKMVDAGLHGRKSGRGFYTY 292 >ref|NP_754359.1| Putative 3-hydroxyacyl-CoA dehydrogenase [Escherichia coli CFT073] gb|AAN80926.1| Putative 3-hydroxyacyl-CoA dehydrogenase [Escherichia coli CFT073] Length = 289 Score = 244 bits (622), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 121/284 (42%), Positives = 170/284 (59%), Gaps = 1/284 (0%) Query: 7 IGIIGAGVMGSDLALDLASQGYRVILKDIHEEALEKAKVKIREDFKLVKLM-KKELPVKK 65 + +IGAGVMG+ +A ++A G + DI + L+K + I + +L + + Sbjct: 4 VAVIGAGVMGTGVAHNMAQYGISTNVVDISQSQLDKCRQMIEANLRLYNFHPQHKKKTHS 63 Query: 66 IEQVLSNIMFTTTYQSFHAVPFVIENITENWEAKQKVYLELQEHCQEDTIYGVNTSCISI 125 +++ NI FTT VIENITE+ E K +Y + C T++GVNTS ISI Sbjct: 64 TAEIMENIRFTTELDDIVECDLVIENITEDIEKKNALYTRMNTICGASTVFGVNTSAISI 123 Query: 126 TKVGAILPRPEHAIGMHFMNPVPLKQMVEIIKGYYTSEDTIRKIKDFLKAIDKKPVLVND 185 T + ++ PE+ +G+HFMNPVPL VE+I+G +T+E T+ ++K ++VND Sbjct: 124 TALSKLMRHPENVVGVHFMNPVPLMHTVELIRGVHTAERTLNIFHHLFAQLNKTGIVVND 183 Query: 186 SPGFVTNRVLMPMINECIWAVHDGVAAPQDVDKIFRLGFSHKMGPLATADLIGLDTILNS 245 SPGFVTNR +M +NE I+ V + +A +D+D +F+ F HKMGPL TADLIGLDTIL S Sbjct: 184 SPGFVTNRAMMIFVNEAIFMVQEQIARAEDIDTLFKTCFGHKMGPLQTADLIGLDTILQS 243 Query: 246 ILVLYESYNDPKYRPCPLLVKMVDAXXXXXXXXXXFFDYTSVLA 289 + VLYES+ND KYRP LL KMVDA F+ Y A Sbjct: 244 LQVLYESFNDDKYRPSFLLKKMVDAGYLGVKSGQGFYRYQQTYA 287 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.322 0.138 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 414,142,497 Number of extensions: 16649945 Number of successful extensions: 59903 Number of sequences better than 1.0e-05: 985 Number of HSP's gapped: 58532 Number of HSP's successfully gapped: 1016 Length of query: 293 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 169 Effective length of database: 420,565,302 Effective search space: 71075536038 Effective search space used: 71075536038 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)