BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0018.p (426 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_242736.1| BH1870~unknown conserved protein in others [Bac... 580 e-164 ref|ZP_00121337.1| hypothetical protein [Bifidobacterium longum ... 331 2e-89 ref|NP_695396.1| possible alpha-galactosidase [Bifidobacterium l... 328 1e-88 dbj|BAB02198.1| unnamed protein product [Arabidopsis thaliana] 199 8e-50 ref|NP_189269.2| glycosyl hydrolase family protein 27 / alpha-ga... 199 8e-50 >ref|NP_242736.1| BH1870~unknown conserved protein in others [Bacillus halodurans] pir||F83883 hypothetical protein BH1870 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05589.1| BH1870~unknown conserved protein in others [Bacillus halodurans] Length = 432 Score = 580 bits (1495), Expect = e-164, Method: Compositional matrix adjust. Identities = 270/422 (63%), Positives = 325/422 (77%), Gaps = 10/422 (2%) Query: 3 HKLAAPTPPLGWNSWDCYGAAVTEDEIRGNAEYMAEHLKDFGWSYITVDIQWYEPHANSS 62 ++L+A TPP+GWNSWDCYGA+VTE+E+ GNAEYMA HLK +GW YI VDIQWYEP ANSS Sbjct: 4 NRLSALTPPMGWNSWDCYGASVTEEEVLGNAEYMANHLKKYGWEYIVVDIQWYEPTANSS 63 Query: 63 QYRPFVPLVMDEYSRLMPAANRFPSAADGQGFKPLADYVHSLGLQFGIHIMRGIPRQAVH 122 Y PF PL MDEY RL+PA NRFPSA +G GFKPL+D +H LGL+FGIHIMRGIPRQAV+ Sbjct: 64 AYNPFAPLCMDEYGRLLPATNRFPSAKNGAGFKPLSDAIHDLGLKFGIHIMRGIPRQAVY 123 Query: 123 AATPILGTTATARDVAHTNSICPWNTDMYGVDASKEGAQAYYDSLFELYAQWGVDLVKVD 182 +P+LG+T TAR++AHTNSICPWNTDMYGVD +KEGAQ+YY+SLFELYAQWGVD VKVD Sbjct: 124 ENSPVLGSTKTAREIAHTNSICPWNTDMYGVDPTKEGAQSYYNSLFELYAQWGVDFVKVD 183 Query: 183 DIAASRLYDTHQPEIALISKAIERCGRPMVLSLSPGPAPVEYAGFFTGHANMWRVTDDFW 242 DIAASRLYDTH EI +I +AI+ CGRPMVLSLSPGPAP++YA F +ANMWR+TDDFW Sbjct: 184 DIAASRLYDTHLEEIKMIQRAIQACGRPMVLSLSPGPAPIKYAHHFKTNANMWRITDDFW 243 Query: 243 DQWPLLLDMFSRCRKWQGVPQAGSWPDCDMLPLGHIGIRSVDGGGADRWTRFTRDEQLTM 302 D W LL MF RC W+ G WPDCDMLPLGHIGIRSVDG G DRWTRFT+DEQLTM Sbjct: 244 DDWSLLYQMFERCEVWEKHIGTGHWPDCDMLPLGHIGIRSVDGPGGDRWTRFTKDEQLTM 303 Query: 303 MSLWSIFRSPLIFGGELRDNDDWTLSLLTNRGVLRMHRESYGAKEALCKDELIVWTAGHT 362 M+LW+I RSPL+FGGELRDND+WTLSLLTN G+L ++++S + +++ + W A Sbjct: 304 MNLWAICRSPLMFGGELRDNDEWTLSLLTNEGILSINQKSVLNRFVYREEDKVAWAANGR 363 Query: 363 DGTRYAAVFNVGESPLQLDLALEEIGLSGTAAGTELW------SGTPAELNAGSLQTTIP 416 +G Y A+FN+ + L L+ +G+ T +W S P+E S Q + Sbjct: 364 NGEAYVALFNLHDQQKTLQFRLDMVGIMETVQLFNVWDRSFLQSLAPSE----SFQIELK 419 Query: 417 PH 418 PH Sbjct: 420 PH 421 >ref|ZP_00121337.1| hypothetical protein [Bifidobacterium longum DJO10A] Length = 469 Score = 331 bits (848), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 189/454 (41%), Positives = 259/454 (57%), Gaps = 49/454 (10%) Query: 10 PPLGWNSWDCYGAAVTEDEIRGNAEYMAEHLKDFGWSYITVDIQWYEPHANSSQYRPFVP 69 PP+GWNSWD YG +TEDE+ NA +MAEHLK+ GW + +DI WY+P A + Y P Sbjct: 14 PPMGWNSWDSYGTTLTEDELLANARFMAEHLKNAGWDTLVIDIDWYDPTARAHGYNDNAP 73 Query: 70 LVMDEYSRLMPAANRFPSAADGQGFKPLADYVHSLGLQFGIHIMRGIPRQAVHAATPILG 129 L++DEY R +P RFPSAA G+GF PLA VH LGL+ G+H+MRGIPR AV P+ G Sbjct: 74 LILDEYGRQLPDPVRFPSAAGGKGFGPLAAAVHELGLKLGMHMMRGIPRIAVDKNLPVYG 133 Query: 130 TTATARDVAHTNSICPWNTDMYGVDASKEGAQAYYDSLFELYAQWGVDLVKVDDIAASRL 189 T TA+DVA + +C WN D YG++ S GAQA+YD+ +L+A WG+D +KVDD+ Sbjct: 134 TNYTAKDVADLDHVCKWNPDNYGLNQSHPGAQAWYDAQLDLFASWGLDFLKVDDMQTP-- 191 Query: 190 YDTHQPEIA----LISKAIERCGRPMVLSLSPGP-APVEYAGFFTGHANMWRVTDDFWDQ 244 H EIA I+KA + GR + LSLSPG Y F +A MWR++DD WD+ Sbjct: 192 --FHSDEIAAYHRAIAKAEAKYGRSIDLSLSPGGWVATSYVDFLRENAQMWRISDDLWDR 249 Query: 245 WPLLLDMFSRCRKWQGVPQAGSWPDCDMLPLGHIGIRSVDGGGADRWTRFTRDEQLTMMS 304 W + F+R +W G W D DM+P GHIG+R+ G DR +R T DEQ T+++ Sbjct: 250 WEDIYQQFARLARWAPFQTTGHWADADMVPFGHIGLRAERGD--DRQSRLTLDEQKTLLA 307 Query: 305 LWSIFRSPLIFGGELRDNDDWTLSLLTN---RGVL---RMHRESYGAK-------EALCK 351 LW + RSPL+ GG+L ++ ++LL N R VL +RE+ + E+ + Sbjct: 308 LWCMGRSPLMVGGDLPTSNSDAIALLQNPALREVLAGSTNNRETVRERIFGKWWDESTYR 367 Query: 352 DELIVWTAGH---TDGTR-------YAAVFNVGESPLQLDLALEEIGLSGTAAGTELWS- 400 E IVW+A DGTR YAA+F G ++ ++ + G A + W+ Sbjct: 368 GEFIVWSADAADWADGTRSAHHGGHYAALFWTGSDTYEIGRNIQLQSIVGLDARNDDWTL 427 Query: 401 --------GTPAELNAGS------LQTTIPPHGV 420 G PA++ + TIPPHGV Sbjct: 428 ADLYADAPGEPADVRLEGVGADRVITGTIPPHGV 461 >ref|NP_695396.1| possible alpha-galactosidase [Bifidobacterium longum NCC2705] gb|AAN24032.1| possible alpha-galactosidase [Bifidobacterium longum NCC2705] Length = 469 Score = 328 bits (841), Expect = 1e-88, Method: Compositional matrix adjust. Identities = 189/454 (41%), Positives = 259/454 (57%), Gaps = 49/454 (10%) Query: 10 PPLGWNSWDCYGAAVTEDEIRGNAEYMAEHLKDFGWSYITVDIQWYEPHANSSQYRPFVP 69 PP+GWNSWD YG +TEDE+ NA +MAEHLK+ GW + +DI WY+P A + Y P Sbjct: 14 PPMGWNSWDSYGTTLTEDELLANARFMAEHLKNAGWDTLVIDIDWYDPTARAHGYNDNAP 73 Query: 70 LVMDEYSRLMPAANRFPSAADGQGFKPLADYVHSLGLQFGIHIMRGIPRQAVHAATPILG 129 L++DEY R +P RFPSAA G+GF PLA VH LGL+ G+H+MRGIPR AV P+ G Sbjct: 74 LILDEYGRQLPDPVRFPSAAGGKGFGPLAAAVHELGLKLGMHMMRGIPRIAVDKNLPVYG 133 Query: 130 TTATARDVAHTNSICPWNTDMYGVDASKEGAQAYYDSLFELYAQWGVDLVKVDDIAASRL 189 T TA+DVA + +C WN D YG++ S GAQA+YD+ +L+A WG+D +KVDD+ Sbjct: 134 TNYTAKDVADLDHVCKWNPDNYGLNQSHPGAQAWYDAQLDLFASWGLDFLKVDDMQTP-- 191 Query: 190 YDTHQPEIA----LISKAIERCGRPMVLSLSPGP-APVEYAGFFTGHANMWRVTDDFWDQ 244 H EIA I+KA + GR + LSLSPG Y F +A MWR++DD WD+ Sbjct: 192 --FHSDEIAAYHRAIAKAEAKYGRSIDLSLSPGGWVATSYVDFLRENAQMWRISDDLWDR 249 Query: 245 WPLLLDMFSRCRKWQGVPQAGSWPDCDMLPLGHIGIRSVDGGGADRWTRFTRDEQLTMMS 304 W + F+R +W G W D DM+P GHIG+R+ G DR +R T DEQ T+++ Sbjct: 250 WEDIYQQFARLARWAPFQTTGHWADADMVPFGHIGLRAERGD--DRQSRLTLDEQKTLLA 307 Query: 305 LWSIFRSPLIFGGELRDNDDWTLSLLTN---RGVL---RMHRESYGAK-------EALCK 351 LW + RSPL+ GG+L ++ ++LL N R VL +RE+ + E+ + Sbjct: 308 LWCMGRSPLMVGGDLPTSNSDAIALLQNPALREVLAGSTNNRETVRERIFGKWWDESTYR 367 Query: 352 DELIVWTAGH---TDGTR-------YAAVFNVGESPLQLDLALEEIGLSGTAAG------ 395 E IVW+A DGTR YAA+F G ++ ++ + G A Sbjct: 368 GEFIVWSADAADWADGTRSAHHGGHYAALFWTGSDTYEIGRNIQLQSIVGLDARNDDWTL 427 Query: 396 TELWSGTPAELNAGSLQ---------TTIPPHGV 420 ++L++ P E L+ TIPPHGV Sbjct: 428 SDLYADAPGESADVRLEGVGADRVITGTIPPHGV 461 >dbj|BAB02198.1| unnamed protein product [Arabidopsis thaliana] Length = 676 Score = 199 bits (507), Expect = 8e-50, Method: Compositional matrix adjust. Identities = 124/364 (34%), Positives = 188/364 (51%), Gaps = 30/364 (8%) Query: 7 APTPPLGWNSWDCYGAAVTEDEIRGNAEYMAEHLKDFGWSYITVDIQWYEPHANSSQYRP 66 A PP GWNS+D + ++E E +AE +++ L G+ Y+ VD WY + Sbjct: 39 ASFPPRGWNSYDSFCWTISEAEFLQSAEIVSKRLLPHGYQYVVVDYLWYRKKVEGAYVDS 98 Query: 67 FVPLVMDEYSRLMPAANRFPSAADGQGFKPLADYVHSLGLQFGIHIMRGIPRQAVHAATP 126 V+DE+ RL P R+PS+ G+GF +A+ VH +GL+FGIH+M GI QA +A + Sbjct: 99 LGFDVIDEWGRLHPDPGRWPSSRGGKGFTEVAEKVHRMGLKFGIHVMGGISTQAYNANSL 158 Query: 127 IL-----------GTTATARDVAHTNSICPWNTDMYGVDASKEGA-QAYYDSLFELYAQW 174 ++ G A+D+ C W + + +K GA +A+ SL+ YA+W Sbjct: 159 VMDSVKGGAYEESGRQWRAKDIGIKERACVWMSHGFMSVNTKLGAGKAFLRSLYRQYAEW 218 Query: 175 GVDLVKVDDIAASRLYDTHQPEIALISKAIERCGRPMVLSLSPGPAPV-EYAGFFTGHAN 233 GVD +K D + + D + EI +S+ ++ RP++ S+SPG + A + N Sbjct: 219 GVDFIKHDCVFGT---DFNIEEITYVSEVLKELDRPVLYSISPGTSVTPTMAKEVSQLVN 275 Query: 234 MWRVTDDFWDQWPLLLDMFSRCRKWQGVP-------QAGSWPDCDMLPLGHIGIRSVDGG 286 M+R+T D WD W + F R Q SWPD DMLPLG + D G Sbjct: 276 MYRITGDDWDTWKDVTAHFDISRDLSASSMIGARGLQGKSWPDLDMLPLGWL----TDQG 331 Query: 287 ---GADRWTRFTRDEQLTMMSLWSIFRSPLIFGGELRDNDDWTLSLLTNRGVLRMHRESY 343 G R +EQ + M+LWSI +SPL+FGG++R+ D T +L+TN +L ++ S Sbjct: 332 SNVGPHRACNLNLEEQKSQMTLWSIAKSPLMFGGDVRNLDATTYNLITNPTLLEINSYSS 391 Query: 344 GAKE 347 KE Sbjct: 392 NNKE 395 >ref|NP_189269.2| glycosyl hydrolase family protein 27 / alpha-galactosidase family protein / melibiase family protein [Arabidopsis thaliana] Length = 647 Score = 199 bits (507), Expect = 8e-50, Method: Compositional matrix adjust. Identities = 124/364 (34%), Positives = 188/364 (51%), Gaps = 30/364 (8%) Query: 7 APTPPLGWNSWDCYGAAVTEDEIRGNAEYMAEHLKDFGWSYITVDIQWYEPHANSSQYRP 66 A PP GWNS+D + ++E E +AE +++ L G+ Y+ VD WY + Sbjct: 39 ASFPPRGWNSYDSFCWTISEAEFLQSAEIVSKRLLPHGYQYVVVDYLWYRKKVEGAYVDS 98 Query: 67 FVPLVMDEYSRLMPAANRFPSAADGQGFKPLADYVHSLGLQFGIHIMRGIPRQAVHAATP 126 V+DE+ RL P R+PS+ G+GF +A+ VH +GL+FGIH+M GI QA +A + Sbjct: 99 LGFDVIDEWGRLHPDPGRWPSSRGGKGFTEVAEKVHRMGLKFGIHVMGGISTQAYNANSL 158 Query: 127 IL-----------GTTATARDVAHTNSICPWNTDMYGVDASKEGA-QAYYDSLFELYAQW 174 ++ G A+D+ C W + + +K GA +A+ SL+ YA+W Sbjct: 159 VMDSVKGGAYEESGRQWRAKDIGIKERACVWMSHGFMSVNTKLGAGKAFLRSLYRQYAEW 218 Query: 175 GVDLVKVDDIAASRLYDTHQPEIALISKAIERCGRPMVLSLSPGPAPV-EYAGFFTGHAN 233 GVD +K D + + D + EI +S+ ++ RP++ S+SPG + A + N Sbjct: 219 GVDFIKHDCVFGT---DFNIEEITYVSEVLKELDRPVLYSISPGTSVTPTMAKEVSQLVN 275 Query: 234 MWRVTDDFWDQWPLLLDMFSRCRKWQGVP-------QAGSWPDCDMLPLGHIGIRSVDGG 286 M+R+T D WD W + F R Q SWPD DMLPLG + D G Sbjct: 276 MYRITGDDWDTWKDVTAHFDISRDLSASSMIGARGLQGKSWPDLDMLPLGWL----TDQG 331 Query: 287 ---GADRWTRFTRDEQLTMMSLWSIFRSPLIFGGELRDNDDWTLSLLTNRGVLRMHRESY 343 G R +EQ + M+LWSI +SPL+FGG++R+ D T +L+TN +L ++ S Sbjct: 332 SNVGPHRACNLNLEEQKSQMTLWSIAKSPLMFGGDVRNLDATTYNLITNPTLLEINSYSS 391 Query: 344 GAKE 347 KE Sbjct: 392 NNKE 395 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.446 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 708,172,537 Number of extensions: 30912742 Number of successful extensions: 73982 Number of sequences better than 1.0e-05: 83 Number of HSP's gapped: 73814 Number of HSP's successfully gapped: 118 Length of query: 426 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 298 Effective length of database: 409,704,906 Effective search space: 122092061988 Effective search space used: 122092061988 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 127 (53.5 bits)