BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0184.0004.p (401 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_242801.1| spore germination protein KC [Bacillus halodura... 140 5e-32 ref|ZP_00097484.1| hypothetical protein [Desulfitobacterium hafn... 133 7e-30 ref|NP_149183.1| Spore germination protein, GRKC [Clostridium ac... 122 2e-26 ref|NP_349895.1| Spore germination protein GerKC, membrane prote... 116 1e-24 gb|AAB61394.1| germination protein 115 2e-24 >ref|NP_242801.1| spore germination protein KC [Bacillus halodurans] pir||G83891 spore germination protein KC gerKC [imported] - Bacillus halodurans (strain C-125) dbj|BAB05654.1| spore germination protein KC [Bacillus halodurans] Length = 399 Score = 140 bits (353), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 111/404 (27%), Positives = 187/404 (46%), Gaps = 33/404 (8%) Query: 15 KSILLVMAATVLLFSTGCWDRKEVNDLAIVTTAGLDLKNNGEIELTLEIVHPKEVGGDTQ 74 K+ LLV+ LL S+ CWD E++DL+I++ G+D NG +++ +I++P EV D Sbjct: 5 KTQLLVLFFATLLMSS-CWDASELSDLSIISGVGIDKGENGTYKVSFQIINPSEVSADED 63 Query: 75 QXXXXXXXXXXXXXXGSTLIWXXXXXXXXXXXXELQVKLSRTVYWGQLEILVIGEALSRS 134 G+++ + +LSR +Y ++VIGE +R Sbjct: 64 GFQQGSTSTVVISSVGNSIF---------EAVRKASEQLSRRIYSPHTNLVVIGEDFARE 114 Query: 135 Q-LREQLDYLVRDSNVRLRVRPFVCKG-TARDFLASDPPLEKTKADFLG----------G 182 + L + LD+ RD R + + +G TA + L P E+ A + G Sbjct: 115 EGLNKALDWFERDHAFRTNIPMVIARGSTAEEVLLVQSPFERVPAKKIDEAIKTSGAVWG 174 Query: 183 ESKRLFRRAITLNSLVQDLGN-ISKEAFLPYVDTTQDG---GESVPYV----KGYAAFSS 234 E+K + + + +N L N IS + +G G+S P V G A F Sbjct: 175 ETKEVEIQDVIINQLRMGRENTISGIKIVGNFSEAVEGEGLGKSKPPVILQAAGMAMFRD 234 Query: 235 YRMAGLIQGESYSGAKWILKQVKGDVETVKLERPSSPLISLGVLSSSTRIDPKFAG-EIP 293 + + ++ G WI ++ V V L + I++ ++ S+ +++ K E Sbjct: 235 GHLHSWLDHKAARGTNWIDNAIESTV--VTLPCHNDEKIAIEIIQSNAQMEAKMTDQETL 292 Query: 294 RMDIVIEAEMSLVQNTTRFKTTDSKFIRNVEEAAVDKIRKKAEITIKQAQRMKADIFGFG 353 +++ I AE ++ + R T + I VE+ D+IR +A I+ +Q + DIFGFG Sbjct: 293 EINLTIFAEGNIGEVNCRVDLTVIENIHKVEKQLEDEIRSEALEAIQISQEEQIDIFGFG 352 Query: 354 EEIERHNPRKWKHIHDQWERMYPLVQANVQVKVRIRQTGMNSVP 397 EE+ R P +WK + DQW + +Q NVQV IR+TG + P Sbjct: 353 EEVHRKYPDQWKMLKDQWNEHFAELQVNVQVNTFIRRTGFRNNP 396 >ref|ZP_00097484.1| hypothetical protein [Desulfitobacterium hafniense] Length = 389 Score = 133 bits (335), Expect = 7e-30, Method: Compositional matrix adjust. Identities = 93/392 (23%), Positives = 175/392 (44%), Gaps = 28/392 (7%) Query: 17 ILLVMAATVLLFSTGCWDRKEVNDLAIVTTAGLD-LKNNGEIELTLEIVHPKEV------ 69 ++ +M ++L TGCW+++E+N LAIV G+D + +I++T +I +PK + Sbjct: 9 VIFLMCTPLILNITGCWNQRELNSLAIVMGLGIDKAQEPNQIQVTAQIANPKALKSSSLG 68 Query: 70 GGDTQQXXXXXXXXXXXXXXGSTLIWXXXXXXXXXXXXELQVKLSRTVYWGQLEILVIGE 129 G D+Q G TL + + +R +Y+ +++L++G Sbjct: 69 GPDSQ-------AFWNIQNTGDTLF---------SILRDFTHESNRKLYFPHIQVLILGR 112 Query: 130 ALSRSQLREQLDYLVRDSNVRLRVRPFVCKGTARDFLASDPPLEKTKA----DFLGGESK 185 + ++++LD+ RD RL + V +G ARD L P +++ A L + Sbjct: 113 DYAEEGIQKELDFFARDHETRLNIWLVVSEGNARDVLNIKPVIDRIPAINISKLLDDQDP 172 Query: 186 RLFRRAITLNSLVQDLGNISKEAFLPYVDTTQDGGESVPYVKGYAAFSSYRMAGLIQGES 245 LN + DL + + P V+ + GG VKG A F ++ G + Sbjct: 173 NSQTSTSQLNDFLSDLISPTTAPTAPLVEIIRKGGNESLQVKGTAVFKGDKLVGELNKYE 232 Query: 246 YSGAKWILKQVKGDVETVKLERPSSPLISLGVLSSSTRIDPKFAGEIPRMDIVIEAEMSL 305 G W +VK + V+ S +SL ++ S T++ + M + I+ + +L Sbjct: 233 TRGLLWARDEVKSGIIDVECPECGSK-VSLEIIDSKTKVSAEIVDNQVIMKVYIQEKGAL 291 Query: 306 VQNTTRFKTTDSKFIRNVEEAAVDKIRKKAEITIKQAQRMKADIFGFGEEIERHNPRKWK 365 + + +E I+ + K+AQ + ADIFGFGE + + + ++WK Sbjct: 292 AAQYCAENLRTKEKVAAIERNKAAAIQDEVLAAFKKAQELNADIFGFGEVVRKKHLKEWK 351 Query: 366 HIHDQWERMYPLVQANVQVKVRIRQTGMNSVP 397 ++ + W+ ++P + V V I ++G P Sbjct: 352 NMQEHWDEIFPEIIVEVTVDASIERSGRIHYP 383 >ref|NP_149183.1| Spore germination protein, GRKC [Clostridium acetobutylicum] gb|AAK76765.1| Spore germination protein, GRKC [Clostridium acetobutylicum] Length = 394 Score = 122 bits (305), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 101/398 (25%), Positives = 178/398 (44%), Gaps = 17/398 (4%) Query: 11 MHACKSILLVMAATVLLFS--TGCWDRKEVNDLAIVTTAGLD-LKNNGEIELTLEIVHPK 67 M +C+ L + + +L+ T C D +E+N LAIV G+D L+N + E+T+++ Sbjct: 1 MKSCRKKFLKIISCILIAVNLTSCIDSRELNKLAIVLAVGIDKLENCDDTEMTIQLAKLS 60 Query: 68 EVGGDTQQXXXXXXXXXXXXXXGSTLIWXXXXXXXXXXXXELQVKLSRTVYWGQLEILVI 127 + + TL KL R +++ +++V+ Sbjct: 61 -----SNKSGSISSGGSAPSANNVTLNLTETGNGLGKIVKTFNRKLDRQLFFSHNQVIVL 115 Query: 128 GEALSRSQLREQLDYLVRDSNVRLRVRPFVCKGTARDFLASDPPLEKTKADFLG---GES 184 GE+ ++S + + LD+ +R RL V + K +D L+ P +E T+ L E Sbjct: 116 GESAAKSGIAKYLDFFMRYRETRLLVWLLITKSKVKDILSVPPNMEDTQGRNLAELISEQ 175 Query: 185 KRLFR-RAITLNSLVQDLGNISKEAFLPYVD--TTQDGGESVPYVKGYAAFSSYRMAGLI 241 K + AI L + + + + +P V+ T G + A F +M G + Sbjct: 176 KEVSEIPAINLKDFTERIMSKTSAPIIPIVEISTNNSAGTKALRLTSTAVFKKDKMIGTL 235 Query: 242 QGESYSGAKWILKQVKGDVETVKLERPSSPLISLGVLSSSTRIDPKFAGEIPRMDIVIEA 301 + G W L +VK V V L I + + S +I K M I I+ Sbjct: 236 DSQQTRGLLWALNKVKTGVVPVNLPGYKGT-IDVETIHSKGKIAAKLKDNKLIMKINIKQ 294 Query: 302 EMSLV-QNTTRFKTTDSKFIRNVEEAAVDKIRKKAEITIKQAQRMKADIFGFGEEIERHN 360 E +L+ QN++ T F + +E+A + I+K+ E I +++ + ADIFGFG+ I R + Sbjct: 295 EGNLLEQNSSENLATPDNF-KVIEKAEEETIKKQIEAAILKSRDLNADIFGFGDAIYRRH 353 Query: 361 PRKWKHIHDQWERMYPLVQANVQVKVRIRQTGMNSVPV 398 P+ +K I QW + + + V V ++R+ G S P+ Sbjct: 354 PKYFKKIEPQWNKTFKNLNIEVNVNCKLRRNGRISKPI 391 >ref|NP_349895.1| Spore germination protein GerKC, membrane protein [Clostridium acetobutylicum] pir||H97305 spore germination protein GerKC, membrane protein [imported] - Clostridium acetobutylicum gb|AAK81235.1| Spore germination protein GerKC, membrane protein [Clostridium acetobutylicum] Length = 383 Score = 116 bits (290), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 103/398 (25%), Positives = 178/398 (44%), Gaps = 26/398 (6%) Query: 11 MHACKSILLVMAATVLLFSTGCWDRK-EVNDLAIVTTAGLDLKNNGEIELTLEIVHPKEV 69 M SI L++ +L GC D K E+N L++V +G+DL +G T+EI++ V Sbjct: 1 MRKLISIFLILCTFLL---CGCSDVKHELNRLSLVLCSGIDLSEDGRYIYTVEILN---V 54 Query: 70 GGDTQQXXXXXXXXXXXXXXGSTLIWXXXXXXXXXXXXELQVKLSRTVYWGQLEILVIGE 129 G + Q S ++ V +++ ++V+GE Sbjct: 55 GASSPQSSGKQGASP------SINVFSSEGHTITEAFNNASVLSGEPLFFAHSRMVVVGE 108 Query: 130 ALSRSQLREQLDYLVRDSNVRLRVRPFVCKGTARDFLASDPPLEKTKADFLGGES--KRL 187 L++ L + +D L R +R FV KG ARD +++ P + A+ L S + + Sbjct: 109 RLAKYGLSDMMDKLFRHYTIRPDGLIFVTKGNARDIVSTYTPDQPLPAEKLRTMSMLQEM 168 Query: 188 FRRAITLN--SLVQDLGNISKEAFLPYVDTTQDGGESVPY-VKGYAAFSSYRMAGLIQGE 244 +I+ + LV+ L + S + + D S + + G F ++ G + Sbjct: 169 TGYSISYSRLDLVKALTSKSHAGAIGLISLRPDQKFSNRFNMYGAGVFKKDKLVGYLDTY 228 Query: 245 SYSGAKWILKQVKGDVETVKLERPSSPLISLGVLSSSTRIDPKFAGEIPRMDIVIEAEMS 304 G +W+ + V L P I ++ S ++I K G M+I + AE S Sbjct: 229 ETRGLQWVTGHISSGSILVPL--PDGKRIVFDIIDSKSKIKTKVNGNKLTMEINV-AEES 285 Query: 305 LVQNTTRFKTTDSKFIRNVEEAA---VDKIRKKAEITIKQAQ-RMKADIFGFGEEIERHN 360 L+ T KT K + + + A + I+K+ + +Q R+K DIFGFG EI R N Sbjct: 286 LISETND-KTNVMKHYKEMNKLAKLESEAIKKEVSSALAASQDRLKTDIFGFGSEIYRDN 344 Query: 361 PRKWKHIHDQWERMYPLVQANVQVKVRIRQTGMNSVPV 398 P+ WK + + W+ ++P V + VK +R+ G+ S P+ Sbjct: 345 PKYWKTVENNWDNIFPNVNVKINVKCIVRRPGLTSQPI 382 >gb|AAB61394.1| germination protein Length = 408 Score = 115 bits (288), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 94/403 (23%), Positives = 176/403 (43%), Gaps = 32/403 (7%) Query: 15 KSILLVMAATVLLFSTGCWDRKEVNDLAIVTTAGLDLKNNGEIELTLEIVHPKEVGGDTQ 74 KS+ L++ +++ T CW +KE+ DL+ V+ G+D +G ELT +I++P V G Sbjct: 4 KSLFLLVMTIMMVLLTSCWSKKELTDLSFVSAMGIDKTKDGRYELTFQIINPGNVAGLQG 63 Query: 75 QXXXXXXXXXXXXXXGSTLIWXXXXXXXXXXXXELQVKLSRTVYWGQLEILVIGEALSRS 134 G L+ ++SR +Y+ ++VIGE L+R Sbjct: 64 GGGTQSPPVTIYSASGDNLV---------EASRRASARISRRLYYAHTNLVVIGEKLARE 114 Query: 135 Q-LREQLDYLVRDSNVRLRVRPFVC-KGTARDFLASDPPLEKTKADFLGGESKRLFRR-- 190 + + +D + RD R+ + + +A D + + P++K A+ + + R+ Sbjct: 115 EGINILIDSIDRDPEFRITTSLVIANQSSAADLVKALTPVDKIPANKIMKTLEFTQRKWG 174 Query: 191 ---AITLNSLVQDLGNISKEAFLPYV-------------DTTQDGGESVPYVKGYAAFSS 234 ++ +++ L + EA + + Q E+ G A F + Sbjct: 175 ENLKVSFQEVMRSLESPGGEALVSGFRLVGNPQQAEKLDNLQQSAPEATLRASGIAVFKN 234 Query: 235 YRMAGLIQGESYSGAKWILKQVKGDVETVKLERPSSPLISLGVLSSSTRIDPKFAGEIPR 294 ++ + G+ WIL +++G V + L + + T + K P Sbjct: 235 GKLIDWLYNSPARGSVWILDKIQGTDINVDWKEKKEAL-AYQTVRQKTDVSVKMIKGKPY 293 Query: 295 MDIVIEAEMSLVQNTTRFKTTDSKFIRNVEEAAVDKIRKKAEITIKQAQRMKADIFGFGE 354 + E L + T I+ +E+AA +I+K+ + IKQAQ K DIFGFGE Sbjct: 294 ASVHTRVEGDLGEVEVPVDTRQPAVIKKMEKAAGKEIKKELQTAIKQAQTDKVDIFGFGE 353 Query: 355 EIERHNPRKWKHIHDQWER--MYPLVQANVQVKVRIRQTGMNS 395 +ER P +W+ + +W+ +P + +V V+ IR+TG+ + Sbjct: 354 ALERTRPNQWEKLKSEWDDDVYFPKLDVDVTVEAYIRRTGLRN 396 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 513,381,477 Number of extensions: 19151235 Number of successful extensions: 45475 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 45449 Number of HSP's successfully gapped: 32 Length of query: 401 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 273 Effective length of database: 409,704,906 Effective search space: 111849439338 Effective search space used: 111849439338 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)