BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0183.0001.p (1288 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060097.1| hypothetical protein [Clostridium thermocellu... 321 8e-86 dbj|BAC45001.1| xylanase 5 [Paenibacillus sp. W-61] 279 4e-73 ref|ZP_00060727.1| hypothetical protein [Clostridium thermocellu... 249 3e-64 dbj|BAA12826.1| beta-1,4-endoglucanase [Clostridium josui] 141 1e-31 gb|AAL79562.1| beta-1,4-glucanase [Clostridium cellulolyticum] 136 3e-30 >ref|ZP_00060097.1| hypothetical protein [Clostridium thermocellum ATCC 27405] pir||JC6031 scaffoldin dockerin binding protein precursor - Clostridium thermocellum gb|AAB07763.1| SdbA Length = 631 Score = 321 bits (822), Expect = 8e-86, Method: Compositional matrix adjust. Identities = 178/390 (45%), Positives = 240/390 (61%), Gaps = 16/390 (4%) Query: 914 KVTMQATVNNGTA----------FVKVLDLAKAKEFMEKNVTLDIPAAQGVNSYSVGLPA 963 KV ++ ++N T K LD AK E +K V L+I + ++Y LPA Sbjct: 243 KVELEPVLDNATGEAKAAIDEEKLNKALDEAKKSE-DDKLVELNIKKVENADAYIQQLPA 301 Query: 964 AALTSGTKDDKLTISTEFGQVVISGNMLTGTTES----SGKEVTMEIGKGDKAKLPAEVK 1019 L + KL I+TE G + + NML S + V I K +L AE+K Sbjct: 302 KFLIKSDAEYKLRIATEQGIIEVPANMLNTADISKLVKNDSVVEFVIRKVKVDELGAELK 361 Query: 1020 AALGDRPVIQLSLKVDGKETAWSNPFAPVTVSVPYKPSADELKNPEMIVVWYIDGSGDVL 1079 +G+RPVI +S+ VDGK+ WSN A V +S+PYKP A EL+N E IVV +ID +G + Sbjct: 362 EKIGNRPVIDISVVVDGKKVEWSNYKAKVKISIPYKPDAKELENHEHIVVLHIDDAGKAV 421 Query: 1080 TVPSGRYAPKTGMVTFTTTHFSSYAVAYVSRTFTDLGTAVWAKNAVEVLASKDILK-TEG 1138 +VPSG+Y P G+VTF T H S YAV+YV +TF D+G+ WAK +EVLASK ++ T Sbjct: 422 SVPSGKYEPSLGVVTFETNHLSKYAVSYVYKTFADIGSYAWAKKQIEVLASKGVINGTSD 481 Query: 1139 HVFNPSTGITRADFLYSLVRALGLNARVNGNFSDVQKSAYYYNEIAIAKALGITNGIDND 1198 F P ITRADF+ LV+ALGL A V NF DV + YYY + IAK LGIT G+ N+ Sbjct: 482 TTFTPQADITRADFMILLVKALGLTAEVTSNFDDVSEKDYYYEYVGIAKELGITTGVGNN 541 Query: 1199 RFGSAYKITRQDMMVLTERALKLEKKLSNQAEAADLERFTDKSEVASYAVNSVAAMVKEG 1258 +F KITRQDMMVLT AL++ K+S+ AD+ERF+DK ++ASYAV VA +VKEG Sbjct: 542 KFNPKAKITRQDMMVLTTNALRIAGKISSTGTRADVERFSDKDQIASYAVEGVATLVKEG 601 Query: 1259 LIEGSGNKVNPAGNTTKAEAAVFLYRLYNK 1288 ++ GSG+ +NP GN ++AE A +Y++Y K Sbjct: 602 IVVGSGDIINPRGNASRAELAAIIYKIYYK 631 >dbj|BAC45001.1| xylanase 5 [Paenibacillus sp. W-61] Length = 1326 Score = 279 bits (713), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 157/382 (41%), Positives = 235/382 (61%), Gaps = 8/382 (2%) Query: 909 VVQGQKVTMQATVNNGTAFVKVLDLAKAKEFMEKNVTLDIPAAQGVNSYSVGLPAAALTS 968 VV+ + T AT+++ + K ++ A +K V++++P SY V LP ++L S Sbjct: 949 VVKVENGTATATISSDS-LNKAIEQASPGTNGKKQVSIEVPKQANAQSYVVQLPTSSLQS 1007 Query: 969 GTKDDKLTISTEFGQVVISGNMLTGTTESSGKEVTMEIGKGDKAKLPAEVKAALGDRPVI 1028 + + + T+ + I NML+ TE + +V+++I K +L AEV+A +G RPVI Sbjct: 1008 -NNNVEFVLKTDHATLQIPSNMLSNMTEQA-DQVSIQIRKVLADRLDAEVRAKVGSRPVI 1065 Query: 1029 QLSLKVDGKETAWSNPFAPVTVSVPYKPSADELKNPEMIVVWYIDGSGDVLTVPSGRYAP 1088 LS+K + AW+NP APVTVSVPY P+ EL N +V+ ID G + VP+GRY Sbjct: 1066 DLSIKAGDRVLAWNNPAAPVTVSVPYTPAGQELVNAHQLVILNIDSQGKTIVVPNGRYDA 1125 Query: 1089 KTGMVTFTTTHFSSYAVAYVSRTFTDLGTAVWAKNAVEVLASKDILK-TEGHVFNPSTGI 1147 K+G F TT FS+YAVAYV+++F D+ +WAK A+E +A++DI+K F P+ I Sbjct: 1126 KSGSAVFKTTEFSTYAVAYVTKSFADVQHVLWAKEAIEAMAARDIIKGISDESFAPAASI 1185 Query: 1148 TRADFLYSLVRALGLNARVN--GNFSDVQKSAYYYNEIAIAKALGITNGIDNDRFGSAYK 1205 TRADF+ LVRAL L + FSDVQ +AYY +AIAK LGI +G +++ F Sbjct: 1186 TRADFITLLVRALELKGKDTNAAAFSDVQSTAYYAQAVAIAKELGIASGFEDNTFKPGSS 1245 Query: 1206 ITRQDMMVLTERALKLEKKLSNQAEAADLERFTDKSEVASYAVNSVAAMVKEGLIEGSGN 1265 I+RQDMMVLT +ALK K S + +L ++D + +++YAV+SV ++V G++ G G Sbjct: 1246 ISRQDMMVLTAKALKAAGKPSTG--SGNLAPYSDAASISTYAVDSVTSLVGSGIVNGKGG 1303 Query: 1266 KVNPAGNTTKAEAAVFLYRLYN 1287 K+ P + T+AEAAV L R++N Sbjct: 1304 KIAPTESLTRAEAAVILSRIWN 1325 >ref|ZP_00060727.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 2547 Score = 249 bits (637), Expect = 3e-64, Method: Compositional matrix adjust. Identities = 136/349 (38%), Positives = 195/349 (55%), Gaps = 2/349 (0%) Query: 942 KNVTLDIPAAQGVNSYSVGLPAAALTSGTKDDKLTISTEFGQVVISGNMLTGTTESSGKE 1001 K V+++I A GV Y LP++ T K+ I T V I +M + Sbjct: 2193 KKVSIEIKKADGVEVYEQKLPSSIFTDTESGRKIEIKTPVATVEIPLDMFEEKDIEKAES 2252 Query: 1002 VTMEIGKGDKAKLPAEVKAALGDRPVIQLSLKVDGKETAWSNPFAPVTVSVPYKPSADEL 1061 +++ + K + + L E KA +GDRPVIQL + VDG+ W + + +SV Y P DEL Sbjct: 2253 ISVRVSKVEASDLSMEQKAQIGDRPVIQLEVLVDGRLINWRSNKTSIKISVDYTPKGDEL 2312 Query: 1062 KNPEMIVVWYIDGSGDVLTVPSGRYAPKTGMVTFTTTHFSSYAVAYVSRTFTDLGTAVWA 1121 K + I VWY+ SG + +P Y G V F+ YAVAY ++F DL WA Sbjct: 2313 KKTDRIFVWYVGDSGKLSAIPMAVYKEDAGKVIFSVQQSGKYAVAYRYKSFDDLKGYDWA 2372 Query: 1122 KNAVEVLASKDILK-TEGHVFNPSTGITRADFLYSLVRALGLNARVNGNFSDVQKSAYYY 1180 K +E+LASK I+ T ++P ITRAD + +V+ALGL A NF DV YYY Sbjct: 2373 KEQIEILASKGIINGTSSTKYSPGLNITRADAVILIVKALGLEAEFTENFDDVSADKYYY 2432 Query: 1181 NEIAIAKALGITNGIDNDRFGSAYKITRQDMMVLTERALKLEKKLSNQAEAADLERFTDK 1240 + IA++ GI G+ N++F ITRQ++MV+ RALK+ + + ++LE F D Sbjct: 2433 EAVGIARSFGIVTGVGNNKFNPETPITRQELMVIVNRALKVVGINLDTGDVSELEAFKDF 2492 Query: 1241 SEVASYAVNSVAAMVKEGLIEGSGNK-VNPAGNTTKAEAAVFLYRLYNK 1288 SE++ YAV SVAA+VK G+I+G NK + P N T+AE AV +Y+L+ K Sbjct: 2493 SEISPYAVESVAALVKAGIIKGDDNKLIAPLRNITRAETAVIIYKLFEK 2541 >dbj|BAA12826.1| beta-1,4-endoglucanase [Clostridium josui] Length = 930 Score = 141 bits (356), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 73/165 (44%), Positives = 108/165 (65%), Gaps = 1/165 (0%) Query: 1123 NAVEVLASKDILKT-EGHVFNPSTGITRADFLYSLVRALGLNARVNGNFSDVQKSAYYYN 1181 N++ +L SK I+ + N ++ I+R +FL +V+ L LNA+ + NFSDV K YYN Sbjct: 761 NSITMLVSKGIINNADVKKINFNSSISRGEFLMWIVKTLDLNAKFSSNFSDVNKKGSYYN 820 Query: 1182 EIAIAKALGITNGIDNDRFGSAYKITRQDMMVLTERALKLEKKLSNQAEAADLERFTDKS 1241 + IAKALGIT+G+ N++F I+R+DM+VLT +ALK+ K + A DL++FTD S Sbjct: 821 SVGIAKALGITSGVGNNKFNPNKAISREDMLVLTYKALKIVNKNLAKGNANDLKQFTDAS 880 Query: 1242 EVASYAVNSVAAMVKEGLIEGSGNKVNPAGNTTKAEAAVFLYRLY 1286 +V+ AV SVA +VK G G K+NP + K+EAA+ LY++Y Sbjct: 881 KVSKNAVESVATIVKNGFYSGDAKKLNPKASVAKSEAALMLYKIY 925 >gb|AAL79562.1| beta-1,4-glucanase [Clostridium cellulolyticum] Length = 930 Score = 136 bits (343), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 73/165 (44%), Positives = 107/165 (64%), Gaps = 1/165 (0%) Query: 1123 NAVEVLASKDILKTEG-HVFNPSTGITRADFLYSLVRALGLNARVNGNFSDVQKSAYYYN 1181 NA+ +L SK I+K+ N + I+R +FL LV+ L LNA+ + NFSDV K YYN Sbjct: 761 NAIAMLVSKGIIKSSDVKKINLNKSISRGEFLNWLVKTLDLNAKYSVNFSDVHKKDSYYN 820 Query: 1182 EIAIAKALGITNGIDNDRFGSAYKITRQDMMVLTERALKLEKKLSNQAEAADLERFTDKS 1241 + IAKALGITNG+ +++F I+R+DM+VLT +ALKL K + A DL++FTD S Sbjct: 821 PVGIAKALGITNGVGHNKFNPNKAISREDMLVLTFKALKLVNKNLVKGNADDLKQFTDAS 880 Query: 1242 EVASYAVNSVAAMVKEGLIEGSGNKVNPAGNTTKAEAAVFLYRLY 1286 +V+ +V S++ +VK G G K+N + +KAE A+ LY++Y Sbjct: 881 KVSRNSVESISTIVKNGFYSGDAKKLNLKASVSKAETALMLYKIY 925 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,878,050,221 Number of extensions: 81164495 Number of successful extensions: 152815 Number of sequences better than 1.0e-05: 68 Number of HSP's gapped: 152730 Number of HSP's successfully gapped: 69 Length of query: 1288 Length of database: 757,237,578 Length adjustment: 138 Effective length of query: 1150 Effective length of database: 382,553,916 Effective search space: 439937003400 Effective search space used: 439937003400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)