BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0021.p (429 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO66291.1| dihydropyrimidine dehydrogenase [Brevibacillus agri] 622 e-177 gb|EAJ19912.1| unknown [environmental sequence] 452 e-125 ref|ZP_00033317.1| COG0167: Dihydroorotate dehydrogenase [Burkho... 449 e-125 ref|ZP_00006802.1| COG0167: Dihydroorotate dehydrogenase [Rhodob... 446 e-124 ref|NP_746165.1| dihydroorotate dehydrogenase family protein [Ps... 441 e-122 >gb|AAO66291.1| dihydropyrimidine dehydrogenase [Brevibacillus agri] Length = 425 Score = 622 bits (1604), Expect = e-177, Method: Compositional matrix adjust. Identities = 298/418 (71%), Positives = 338/418 (80%), Gaps = 1/418 (0%) Query: 1 MADLSIDLAGIKSPNPFWLASAPPTNTGYQVQRAFEAGWGGAVWKTLGDPIINTSSRFAA 60 MADLSI+LAGI SPNPFWLASAPPTN+GYQVQRAFEAGWGGAVWKTLG+PIIN +SRFA Sbjct: 1 MADLSINLAGITSPNPFWLASAPPTNSGYQVQRAFEAGWGGAVWKTLGEPIINVTSRFAG 60 Query: 61 VHFNGQRVAGFNNIELITDRPLEVNLKEIYETKKRFPNHAVVASLMVEPKQEKWHEIVKR 120 + F GQRV GFNNIELITD+PLEVNLKE+ ETKKRFP H ++ASLMVE KQE WHEIVK+ Sbjct: 61 LTFGGQRVFGFNNIELITDKPLEVNLKEMDETKKRFPKHTLIASLMVEHKQEAWHEIVKK 120 Query: 121 AEAVGVDGLELNFGCPHGMAERGMGAASGQQPDLVEAQTYWVKEVAATPVIVKLTPNITD 180 EA+GVDGLELNFGCPHGMAERGMG+A GQ PDL+ Q WVKEVA TPVIVKLTPNITD Sbjct: 121 VEAIGVDGLELNFGCPHGMAERGMGSAVGQHPDLIRQQVEWVKEVAQTPVIVKLTPNITD 180 Query: 181 ITVVARHAVKGGADAISLINTINSLAGVDIHSWNTIPNVGGQGAHGGYCGPAVKPIALSM 240 I AR A GGADAIS+INTINSL GVD+ W IP+V G+GAHGGYCGPAVKPIAL+M Sbjct: 181 IRFTARAASAGGADAISMINTINSLMGVDLDKWLPIPHVDGKGAHGGYCGPAVKPIALNM 240 Query: 241 VAECARDRXXXXXXXXXXXXXTWQDVVEFMLMGSTGIQVCTAVMHHGFRIVGEMIDGLNN 300 VAECARD W+D VEF+LMG+TG+QVCTA MHHGFRIV +MIDGLNN Sbjct: 241 VAECARDPFVGVPISGIGGISNWRDAVEFLLMGATGVQVCTAAMHHGFRIVEDMIDGLNN 300 Query: 301 YLDDKGLASVTELIGKSVPKYSNWGDLDLNYKVVARXXXXXXXXXXKCHIACEDASHQCI 360 YLD + +ASV +++GK+V YS+WG L+LNYKVVAR KCHIACED SHQCI Sbjct: 301 YLDQRKIASVMDIVGKAVHTYSDWGHLNLNYKVVARIHEETCINCNKCHIACEDTSHQCI 360 Query: 361 DMLTDAG-GKAILHVREEDCVGCNLCSIVCPADGAIDMVALDSGAVPMSWNQRQKVIS 417 D++ DA GK L VREEDCVGCNLCSIVCP +G I+MV + +G P+SWNQRQ ++ Sbjct: 361 DIVKDAATGKERLVVREEDCVGCNLCSIVCPVEGTIEMVEIPTGKPPLSWNQRQAALA 418 >gb|EAJ19912.1| unknown [environmental sequence] Length = 442 Score = 452 bits (1162), Expect = e-125, Method: Compositional matrix adjust. Identities = 228/436 (52%), Positives = 294/436 (67%), Gaps = 10/436 (2%) Query: 1 MADLSIDLAGIKSPNPFWLASAPPTNTGYQVQRAFEAGWGGAVWKTLG-DP-IINTSSRF 58 MADL +AGI SPNPFWLASAPPT+ Y V RAFEAGWGG VWKTLG DP ++N SSR+ Sbjct: 1 MADLRCTIAGITSPNPFWLASAPPTDKAYNVNRAFEAGWGGVVWKTLGLDPHVVNVSSRY 60 Query: 59 AAVHFNGQRVAGFNNIELITDRPLEVNLKEIYETKKRFPNHAVVASLMVEPKQEKWHEIV 118 AV +NGQR+AG NNIELITDRPL+VNLKEI + K+ +P A++ SLMV + W I+ Sbjct: 61 GAVQWNGQRIAGLNNIELITDRPLDVNLKEIAQVKRDWPERAMIVSLMVPCNERDWKWIL 120 Query: 119 KRAEAVGVDGLELNFGCPHGMAERGMGAASGQQPDLVEAQTYWVKEVAATPVIVKLTPNI 178 E G D +ELNFGCPHGM+ERGMGAA GQ P+ +E T WVKE + P +VKLTPNI Sbjct: 121 PLVEDTGADAVELNFGCPHGMSERGMGAAVGQVPEYIEMVTRWVKEGSKLPCLVKLTPNI 180 Query: 179 TDITVVARHAVKGGADAISLINTINSLAGVDIHSWNTIPNVGGQGAHGGYCGPAVKPIAL 238 +DI + +R A KGGAD +SLINTINS+ VD+ + + +P V G+G HGGYCGPAVKPIAL Sbjct: 181 SDIRLGSRAAYKGGADGVSLINTINSIVAVDLDAMSPLPMVDGKGTHGGYCGPAVKPIAL 240 Query: 239 SMVAECARD-RXXXXXXXXXXXXXTWQDVVEFMLMGSTGIQVCTAVMHHGFRIVGEMIDG 297 +MVAE ARD TW+D EFM++G+ +QVCTA MH+GFRIV ++ DG Sbjct: 241 NMVAEIARDVETPNLPISGIGGISTWRDAAEFMVLGAGSVQVCTAAMHYGFRIVSDLADG 300 Query: 298 LNNYLDDKGLASVTELIGKSVPKYSNWGDLDLNYKVVARXXXXXXXXXXKCHIACEDASH 357 L+N++D+KG A++ ++ G++VP ++W L+L Y + AR CHIACED +H Sbjct: 301 LSNWMDEKGYATLDDIRGRAVPNVTDWQYLNLKYDIKARIDQDKCIQCGLCHIACEDTAH 360 Query: 358 QCIDMLTDAGGKAILHVREEDCVGCNLCSIVCPADGAIDMVALDSGAVPMSW----NQRQ 413 Q I + + G V + +CVGCNLC VCP + I M +DSG +W N Sbjct: 361 QAI--MKEKDGVRHFEVMDSECVGCNLCMHVCPVEQCITMERVDSGEY-ANWTTHPNNPA 417 Query: 414 KVISSLNGSYSEAEVV 429 +V ++ G+ AE V Sbjct: 418 RVNAATGGTSDAAEAV 433 >ref|ZP_00033317.1| COG0167: Dihydroorotate dehydrogenase [Burkholderia fungorum] Length = 519 Score = 449 bits (1155), Expect = e-125, Method: Compositional matrix adjust. Identities = 220/406 (54%), Positives = 282/406 (69%), Gaps = 5/406 (1%) Query: 1 MADLSIDLAGIKSPNPFWLASAPPTNTGYQVQRAFEAGWGGAVWKTLG-DP-IINTSSRF 58 MADL +AGI SPNPFWLASAPPT+ Y V RAFEAGWGG VWKTLG DP ++N SSR+ Sbjct: 76 MADLRCTIAGITSPNPFWLASAPPTDKAYNVNRAFEAGWGGVVWKTLGLDPHVVNVSSRY 135 Query: 59 AAVHFNGQRVAGFNNIELITDRPLEVNLKEIYETKKRFPNHAVVASLMVEPKQEKWHEIV 118 AV +NGQR+AG NNIELITDRPL+VNLKEI + K+ +P+ A++ SLMV + W I+ Sbjct: 136 GAVQWNGQRIAGLNNIELITDRPLDVNLKEIAQVKRDWPDRAMIVSLMVPCNERDWKWIL 195 Query: 119 KRAEAVGVDGLELNFGCPHGMAERGMGAASGQQPDLVEAQTYWVKEVAATPVIVKLTPNI 178 E G D +ELNFGCPHGM+ERGMGAA GQ P+ VE T WVKE P +VKLTPNI Sbjct: 196 PLVEDTGADAVELNFGCPHGMSERGMGAAVGQVPEYVEMVTRWVKEGTKLPCLVKLTPNI 255 Query: 179 TDITVVARHAVKGGADAISLINTINSLAGVDIHSWNTIPNVGGQGAHGGYCGPAVKPIAL 238 +DI + +R A KGGAD +SLINTINS+ VD+ + + +P V G+G HGGYCGPAVKPIAL Sbjct: 256 SDIRLGSRAAYKGGADGVSLINTINSIVAVDLDAMSPLPMVDGKGTHGGYCGPAVKPIAL 315 Query: 239 SMVAECARD-RXXXXXXXXXXXXXTWQDVVEFMLMGSTGIQVCTAVMHHGFRIVGEMIDG 297 +MVAE ARD +W+D EFM++G+ +QVCTA MH+GFRIV ++ DG Sbjct: 316 NMVAEIARDAETPNLPISGIGGISSWRDAAEFMVLGAGSVQVCTAAMHYGFRIVSDLADG 375 Query: 298 LNNYLDDKGLASVTELIGKSVPKYSNWGDLDLNYKVVARXXXXXXXXXXKCHIACEDASH 357 L+N++D+KG A++ ++ G++VP ++W L+L Y + AR CHIACED +H Sbjct: 376 LSNWMDEKGYATLDDIRGRAVPNVTDWKYLNLKYDIKARIDQDKCIQCGLCHIACEDTAH 435 Query: 358 QCIDMLTDAGGKAILHVREEDCVGCNLCSIVCPADGAIDMVALDSG 403 Q I + + G V + +CVGCNLC VCP + I M +D+G Sbjct: 436 QAI--MKEKDGVRHFEVMDSECVGCNLCMHVCPVEQCITMERVDNG 479 >ref|ZP_00006802.1| COG0167: Dihydroorotate dehydrogenase [Rhodobacter sphaeroides] Length = 434 Score = 446 bits (1147), Expect = e-124, Method: Compositional matrix adjust. Identities = 222/410 (54%), Positives = 279/410 (68%), Gaps = 9/410 (2%) Query: 1 MADLSIDLAGIKSPNPFWLASAPPTNTGYQVQRAFEAGWGGAVWKTLGD---PIINTSS- 56 MA+L D GIKSPNPFWLASAPPT+ Y V+RAFEAGWGG VWKTLG P++N + Sbjct: 1 MANLRSDFIGIKSPNPFWLASAPPTDKEYNVRRAFEAGWGGVVWKTLGSEGPPVVNVNGP 60 Query: 57 RFAAVHFNGQRVAGFNNIELITDRPLEVNLKEIYETKKRFPNHAVVASLMVEPKQEKWHE 116 R+ A++ +R+ G NNIELITDRPLEVNL+EI K+ +P+ A+V SLMV +E W Sbjct: 61 RYGAIYGADRRLLGLNNIELITDRPLEVNLREIKSVKRDYPDRALVVSLMVPCDEESWKA 120 Query: 117 IVKRAEAVGVDGLELNFGCPHGMAERGMGAASGQQPDLVEAQTYWVKEVAATPVIVKLTP 176 I+ E G DG+ELNFGCPHGMAERGMG+A GQ P+ +E T WVK+ + P IVKLTP Sbjct: 121 ILAHVEDTGADGVELNFGCPHGMAERGMGSAVGQVPEYIEMVTRWVKQHSQMPCIVKLTP 180 Query: 177 NITDITVVARHAVKGGADAISLINTINSLAGVDIHSWNTIPNVGGQGAHGGYCGPAVKPI 236 N+TDI A A +GGADA+SLINTINS+ GVDI S+ +P + G+G HGGYCGPAVKPI Sbjct: 181 NVTDIRKPAEAARRGGADAVSLINTINSITGVDIDSFAPMPTIDGKGTHGGYCGPAVKPI 240 Query: 237 ALSMVAECARD-RXXXXXXXXXXXXXTWQDVVEFMLMGSTGIQVCTAVMHHGFRIVGEMI 295 AL+MVAE AR+ TW+D VEFML+G+ +QVCTA M +GFR+V EMI Sbjct: 241 ALNMVAEIARNPETHGLPISGIGGVTTWRDAVEFMLLGAGNVQVCTAAMTYGFRVVQEMI 300 Query: 296 DGLNNYLDDKGLASVTELIGKSVPKYSNWGDLDLNYKVVARXXXXXXXXXXKCHIACEDA 355 GL++Y+D KG AS +L+G++VP ++W L+LNY A +C+ ACED Sbjct: 301 SGLSDYMDAKGFASTADLVGRAVPNVTDWQYLNLNYVTKAEIDQDLCIKCGRCYAACEDT 360 Query: 356 SHQCIDMLTDAGGKAILHVREEDCVGCNLCSIVCPADGAIDMVALDSGAV 405 SHQ I M TD V++E+CV CNLC VCP + I M L GA+ Sbjct: 361 SHQAIAMSTD----RTFTVKDEECVACNLCVDVCPVEDCITMRELPKGAL 406 >ref|NP_746165.1| dihydroorotate dehydrogenase family protein [Pseudomonas putida KT2440] gb|AAN69629.1| dihydroorotate dehydrogenase family protein [Pseudomonas putida KT2440] Length = 424 Score = 441 bits (1133), Expect = e-122, Method: Compositional matrix adjust. Identities = 230/414 (55%), Positives = 273/414 (65%), Gaps = 3/414 (0%) Query: 1 MADLSIDLAGIKSPNPFWLASAPPTNTGYQVQRAFEAGWGGAVWKTLG-DPI-INTSSRF 58 MADLSI AGIK+PNPFWLASAPPT+ Y V RAFEAGWGG VWKTLG DP +N SSR+ Sbjct: 1 MADLSIVFAGIKAPNPFWLASAPPTDKAYNVVRAFEAGWGGVVWKTLGEDPAAVNVSSRY 60 Query: 59 AAVHFNGQRVAGFNNIELITDRPLEVNLKEIYETKKRFPNHAVVASLMVEPKQEKWHEIV 118 +A + ++V G NNIELITDR LE+NL+EI + KK +P+ A++ SLMV +E W I+ Sbjct: 61 SAHYGPNRQVQGINNIELITDRSLEINLREITQVKKDWPDRALIVSLMVPCIEESWKFIL 120 Query: 119 KRAEAVGVDGLELNFGCPHGMAERGMGAASGQQPDLVEAQTYWVKEVAATPVIVKLTPNI 178 EA G DG+ELNFGCPHGM ERGMGAA GQ P+ VE T W K A PVIVKLTPNI Sbjct: 121 PLVEATGADGIELNFGCPHGMPERGMGAAVGQVPEYVEMVTRWCKTYCALPVIVKLTPNI 180 Query: 179 TDITVVARHAVKGGADAISLINTINSLAGVDIHSWNTIPNVGGQGAHGGYCGPAVKPIAL 238 TDI AR A +GGADA+SLINTINS+ VD+ P VG Q HGGYCG AVKPIAL Sbjct: 181 TDIRQSARAAHRGGADAVSLINTINSITSVDLDRMVAHPIVGDQSTHGGYCGSAVKPIAL 240 Query: 239 SMVAECARD-RXXXXXXXXXXXXXTWQDVVEFMLMGSTGIQVCTAVMHHGFRIVGEMIDG 297 +MVAE ARD W+D EFM +GS +QVCTA M HGFRIV +M DG Sbjct: 241 NMVAEIARDPETRGLPICGIGGIGNWRDAAEFMALGSGAVQVCTAAMLHGFRIVEDMQDG 300 Query: 298 LNNYLDDKGLASVTELIGKSVPKYSNWGDLDLNYKVVARXXXXXXXXXXKCHIACEDASH 357 L ++D G A+V G++V ++W LD+NYK VA +CHIACED SH Sbjct: 301 LARWMDQHGHATVEAFRGQAVGHTTDWKYLDINYKSVAHIDQEACIGCGRCHIACEDTSH 360 Query: 358 QCIDMLTDAGGKAILHVREEDCVGCNLCSIVCPADGAIDMVALDSGAVPMSWNQ 411 Q I A G V EE+CVGCNLC I CP + I+M A D+G ++W Q Sbjct: 361 QAIASTLKADGTHAYSVIEEECVGCNLCQITCPVENCIEMEAQDTGKPYLNWTQ 414 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 623,341,038 Number of extensions: 24928314 Number of successful extensions: 65305 Number of sequences better than 1.0e-05: 171 Number of HSP's gapped: 64919 Number of HSP's successfully gapped: 174 Length of query: 429 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 301 Effective length of database: 409,704,906 Effective search space: 123321176706 Effective search space used: 123321176706 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)