BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0020.p (303 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAO66290.1| glutamate synthase small subunit protein [Breviba... 339 4e-92 ref|NP_229440.1| glutamate synthase, beta subunit [Thermotoga ma... 222 1e-56 ref|NP_632688.1| glutamate synthase [NADPH] [Methanosarcina maze... 203 4e-51 ref|NP_618658.1| glutamate synthase (NADPH) [Methanosarcina acet... 196 4e-49 ref|ZP_00061534.1| COG0493: NADPH-dependent glutamate synthase b... 196 7e-49 >gb|AAO66290.1| glutamate synthase small subunit protein [Brevibacillus agri] Length = 448 Score = 339 bits (870), Expect = 4e-92, Method: Compositional matrix adjust. Identities = 170/286 (59%), Positives = 215/286 (75%), Gaps = 2/286 (0%) Query: 1 VVIYEAKELAGGLDTHGIVSFRLPQSIPLWEVEQVRKLGVEIRTGMKVGVDVSVEELKAG 60 V IYEAKE AGGL+T+GIVSFRLPQ I LWEVEQV LGV I+T ++GVD+ +EL A Sbjct: 154 VTIYEAKERAGGLNTYGIVSFRLPQEISLWEVEQVEALGVTIKTNTRIGVDIMPDELVAN 213 Query: 61 YDAIVLAAGMGYVPPLGIEGEKLSGVYDAIELVETTKTGIPALELMGRRVAVIGAGNTAV 120 YD+++LA GM VP L I GE+L GV DAI LVE TKT +++G++V VIGAGNTA+ Sbjct: 214 YDSVLLAVGMSSVPSLHIPGEELEGVLDAIALVEDTKTKPLTTDMVGKKVVVIGAGNTAI 273 Query: 121 DAATCSVRLGAANVKMVYRRTPAEMTAYDFEYEFAKQEGVEFSWLTLPKRIVGDELGNVT 180 DAATCS RLGA NV+++YRRT EM+ Y FEYEFAKQ+GVEF WL P R++G++ G VT Sbjct: 274 DAATCSKRLGADNVQILYRRTVQEMSCYQFEYEFAKQDGVEFRWLVAPTRVLGNK-GRVT 332 Query: 181 ALECVQMKLTGETGKDGRLIPVPVEGSEFLMPVDAVVVAIGQKRRVDLIEALGLEHERGV 240 LE ++M+L GE GR PVP+ GSEF + VD VV AIGQ R + LI+ LGL+H+ G+ Sbjct: 333 GLELIRMEL-GEPDAKGRKRPVPIPGSEFFIEVDFVVKAIGQNRHLSLIDQLGLQHQNGI 391 Query: 241 VKIDEATGRTSDPQIYAAGDIVFGSGKGEATVVSAARQGKDAAYAI 286 V +++ T RTS P+++AAGD++FG GK +A VV AA GK AAYAI Sbjct: 392 VTVEDGTYRTSHPKVFAAGDVIFGGGKTDAMVVDAANHGKRAAYAI 437 >ref|NP_229440.1| glutamate synthase, beta subunit [Thermotoga maritima] pir||H72230 glutamate synthase, beta subunit - Thermotoga maritima (strain MSB8) gb|AAD36707.1| glutamate synthase, beta subunit [Thermotoga maritima] Length = 468 Score = 222 bits (565), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 143/303 (47%), Positives = 181/303 (59%), Gaps = 18/303 (5%) Query: 1 VVIYEAKELAGGLDTHGIVSFRLPQSIPLWEVEQVRKLGVEIRTGMKVGVDVSVEELKAG 60 V I+EA GG+ +GI FRLP+ I EV +RKLGV VG V V+EL + Sbjct: 166 VDIFEAFHKPGGVLVYGIPEFRLPKRIVEREVSYIRKLGVNFHLNTVVGKTVKVKELLSE 225 Query: 61 YDAIVLAAGMGYVPPLGIEGEKLSGVYDAIELVETTKTGIPALELM---------GRRVA 111 YDA+ + G G +GI G L+GVY A E + T+ + L G++VA Sbjct: 226 YDAVFIGTGAGTPKFMGIPGTNLNGVYSANEFL--TRVNLMKAYLFPEYDTPIRVGKKVA 283 Query: 112 VIGAGNTAVDAATCSVRLGAANVKMVYRRTPAEMTAYDFEYEFAKQEGVEFSWLTLPKRI 171 VIGAGNTA+DAA ++RLGA V +VYRRT EM A EY A +EG+EF WLTLP R Sbjct: 284 VIGAGNTAMDAARSALRLGAEKVYIVYRRTEREMPARREEYHHALEEGIEFLWLTLPIRY 343 Query: 172 VGDELGNVTALECVQMKLTGETGKDGRLIPVPVEGSEFLMPVDAVVVAIGQ-KRRVDLIE 230 +GD GNV A+ECV+M+L E GR PVP+EGS F++ VD V+ AIGQ RV L E Sbjct: 344 IGDANGNVEAMECVRMELK-EADGSGRPRPVPIEGSNFVLEVDMVIEAIGQGPNRVLLSE 402 Query: 231 ALGLE-HERGVVKIDEATGRTSDPQIYAAGDIVFGSGKGEATVVSAARQGKDAAYAIMQQ 289 GLE +ERG +K DE TG TS ++A GDIV G+ ATV+ A GK AA I Sbjct: 403 FPGLELNERGYIKADEDTGATSVKGVFAGGDIVTGA----ATVIKAMGAGKKAAQFIHSY 458 Query: 290 MSG 292 ++G Sbjct: 459 LTG 461 >ref|NP_632688.1| glutamate synthase [NADPH] [Methanosarcina mazei Goe1] gb|AAM30360.1| glutamate synthase [NADPH] [Methanosarcina mazei Goe1] Length = 469 Score = 203 bits (516), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 138/299 (46%), Positives = 182/299 (60%), Gaps = 13/299 (4%) Query: 1 VVIYEAKELAGGLDTHGIVSFRLPQSIPLWEVEQVRKLGVEIRTGMKVGVDVSVEELKAG 60 V I+E+ AGG+ ++GI FRLP+ I E+E + KLGVE+R +G +++EL Sbjct: 174 VTIFESLHKAGGVLSYGIPEFRLPKEIVRQEIEYIEKLGVEVRPNYIIGRIKTLDELCDD 233 Query: 61 YDAIVLAAGMGYVPPLGIEGEKLSGVYDAIEL---VETTKTGIPALELMGRR---VAVIG 114 +DA+ L G G +GIEGE L+GVY A E V K P + RR V V+G Sbjct: 234 FDAVFLGTGAGLPSFMGIEGENLNGVYSANEFLTRVNLMKAYDPEYDTTVRRGKNVVVVG 293 Query: 115 AGNTAVDAATCSVRLGAANVKMVYRRTPAEMTAYDFEYEFAKQEGVEFSWLTLPKRIVGD 174 GN A+DAA ++RLGA V +VYRR EM A E E AK+EG+ F LT P RI+GD Sbjct: 294 GGNVAMDAARSALRLGADEVSIVYRRGEDEMPARREEIEHAKEEGITFRLLTNPVRILGD 353 Query: 175 ELGNVTALECVQMKLTGETGKDGRLIPVPVEGSEFLMPVDAVVVAIGQKRRVDLIE-ALG 233 E NVT +EC++M+L GE K GR PVPVEGSEFL+P D VV+AIG + + + G Sbjct: 354 EKFNVTGVECIRMEL-GEPDKSGRRSPVPVEGSEFLVPADVVVIAIGTSPNPIIFKGSEG 412 Query: 234 LE-HERGVVKIDEATGRTSDPQIYAAGDIVFGSGKGEATVVSAARQGKDAAYAIMQQMS 291 LE ++RG V DE TG TS ++A GD+V G+ ATV+SA GK AA AI + + Sbjct: 413 LEQNKRGTVVADEETGATSKCGVFAGGDVVTGA----ATVISAMGAGKKAAKAIDEYLK 467 >ref|NP_618658.1| glutamate synthase (NADPH) [Methanosarcina acetivorans str. C2A] gb|AAM07138.1| glutamate synthase (NADPH) [Methanosarcina acetivorans str. C2A] Length = 469 Score = 196 bits (499), Expect = 4e-49, Method: Compositional matrix adjust. Identities = 135/294 (45%), Positives = 178/294 (60%), Gaps = 13/294 (4%) Query: 1 VVIYEAKELAGGLDTHGIVSFRLPQSIPLWEVEQVRKLGVEIRTGMKVGVDVSVEELKAG 60 V I+E+ AGG+ ++GI FRLP+ I EVE + KLGVE++ +G +++EL Sbjct: 174 VTIFESLHKAGGVLSYGIPEFRLPKEIVRQEVEYIEKLGVEVKPNYIIGRIKTLDELCDD 233 Query: 61 YDAIVLAAGMGYVPPLGIEGEKLSGVYDAIEL---VETTKTGIPALELM---GRRVAVIG 114 +DA+ L G G +GIEGE L+GVY A E V K P + M G+ V V+G Sbjct: 234 FDAVFLVTGAGLPNFMGIEGENLNGVYSANEFLTRVNLMKAYDPEYDTMVRHGKHVVVVG 293 Query: 115 AGNTAVDAATCSVRLGAANVKMVYRRTPAEMTAYDFEYEFAKQEGVEFSWLTLPKRIVGD 174 GN A+DAA ++RLGA V +VYRR EM A E E AK+EG+ F LT P RI+GD Sbjct: 294 GGNVAMDAARSALRLGADEVSIVYRRGEDEMPARREEIEHAKEEGIIFRLLTNPVRILGD 353 Query: 175 ELGNVTALECVQMKLTGETGKDGRLIPVPVEGSEFLMPVDAVVVAIGQKRRVDLIE-ALG 233 E NVT +EC++M+L GE K GR PVPVEG+EFL+ D V++AIG + + + G Sbjct: 354 EKFNVTGVECIKMEL-GEPDKSGRRSPVPVEGTEFLVQADVVIIAIGTSPNPIIFKGSEG 412 Query: 234 LE-HERGVVKIDEATGRTSDPQIYAAGDIVFGSGKGEATVVSAARQGKDAAYAI 286 LE + RG V DE TG TS ++A GD V G+ ATV+SA GK AA AI Sbjct: 413 LEQNRRGTVVADEETGATSKCGVFAGGDAVTGA----ATVISAMGAGKKAAKAI 462 >ref|ZP_00061534.1| COG0493: NADPH-dependent glutamate synthase beta chain and related oxidoreductases [Clostridium thermocellum ATCC 27405] Length = 464 Score = 196 bits (497), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 131/298 (43%), Positives = 182/298 (61%), Gaps = 19/298 (6%) Query: 1 VVIYEAKELAGGLDTHGIVSFRLPQSIPLWEVEQVRKLGVEIRTGMKVGVDVSVEELKA- 59 V I+EA + GG+ +GI FRLP+ + E+E +++LGVEI+T M +G S++ELKA Sbjct: 166 VTIFEAFHVPGGVLMYGIPEFRLPKKLVQEEIETIKQLGVEIKTNMVIGKVYSIDELKAE 225 Query: 60 GYDAIVLAAGMGYVPPLGIEGEKLSGVYDAIELVETTKTGIPALEL--------MGRRVA 111 GYDAI + +G G + I GE L+GVY A E + T + A E +G+ VA Sbjct: 226 GYDAIFIGSGAGLPSFMKIPGENLNGVYSANEFL-TRINLMKAYEFPNCDTPVKVGKNVA 284 Query: 112 VIGAGNTAVDAATCSVRLGAANVKMVYRRTPAEMTAYDFEYEFAKQEGVEFSWLTLPKRI 171 V+G GN A+DAA + RLGA NV +VYRR+ AEM A E AK+EG+ F +LT P RI Sbjct: 285 VVGGGNVAMDAARSAKRLGAENVYIVYRRSEAEMPARLEEIHHAKEEGILFKFLTNPTRI 344 Query: 172 VGDELGNVTALECVQMKLTGETGKDGRLIPVPVEGSEFLMPVDAVVVAIGQKRRVDLIEA 231 +G + G V +EC++M+L GE + GR PVP GSE ++ V+ V++AIGQ LI + Sbjct: 345 LGTDDGWVKGMECIEMEL-GEPDESGRRRPVPKPGSEHVIDVETVIIAIGQTPN-PLIAS 402 Query: 232 L--GLEHER-GVVKIDEATGRTSDPQIYAAGDIVFGSGKGEATVVSAARQGKDAAYAI 286 GL ++ G + +DE TG T+ +YA GD V G+ ATV+ A GK AA AI Sbjct: 403 TTPGLATQKWGGIIVDENTGATNIEGVYAGGDAVTGA----ATVILAMGAGKKAAKAI 456 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 435,882,865 Number of extensions: 18195352 Number of successful extensions: 55025 Number of sequences better than 1.0e-05: 724 Number of HSP's gapped: 53794 Number of HSP's successfully gapped: 727 Length of query: 303 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 178 Effective length of database: 417,850,203 Effective search space: 74377336134 Effective search space used: 74377336134 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)