BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0178.0006.p (505 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||S15713 alpha-amylase (EC 3.2.1.1) - Bacillus circulans >gi|... 847 0.0 ref|ZP_00183205.1| hypothetical protein Exigu144301 [Exiguobacte... 671 0.0 sp|P19571|AMT6_BACS7 Glucan 1,4-alpha-maltohexaosidase precursor... 513 e-144 gb|AAF00567.1| raw starch digesting amylase precursor [Cytophaga... 513 e-144 dbj|BAA32431.1| amylase [Bacillus sp.] 508 e-142 >pir||S15713 alpha-amylase (EC 3.2.1.1) - Bacillus circulans emb|CAA43194.1| amylase [Bacillus circulans] Length = 493 Score = 847 bits (2189), Expect = 0.0, Method: Compositional matrix adjust. Identities = 399/481 (82%), Positives = 438/481 (91%) Query: 1 MMQFFEWHVAADGQHWKRLAQMAPELKAAGIDSVWVPPVTKAISPEDTGYGVYDLYDLGE 60 MMQFFEWH+AADG HWKRLA+MAPELKA GID+VWVPPVTKA+S EDTGYGVYDLYDLGE Sbjct: 7 MMQFFEWHLAADGDHWKRLAEMAPELKAKGIDTVWVPPVTKAVSAEDTGYGVYDLYDLGE 66 Query: 61 FDQKGTIRTKYGTKQELIDAIAECLRNGIAVYVDLVMNHKAGADETEVFEVIEVDPNNRN 120 FDQKGT+RTKYGTKQELI+AIAEC +NGIAVYVDLVMNHKAGADETEVF+VIEVDPN+R Sbjct: 67 FDQKGTVRTKYGTKQELIEAIAECQKNGIAVYVDLVMNHKAGADETEVFKVIEVDPNDRT 126 Query: 121 KEISAPFEIEGWTKFTFPGRGDTYSSFKWNHTHFNGTDFDAKGGRNGVFRINGENKNWNQ 180 KEIS PFEIEGWTKFTFPGRGD YSSFKWN HFNGTDFDA+ R GVFRI GENK WN+ Sbjct: 127 KEISEPFEIEGWTKFTFPGRGDQYSSFKWNSEHFNGTDFDAREERTGVFRIAGENKKWNE 186 Query: 181 NVDDEFGNYDYLMFANIDYSQQEVRREMLEWGKWLVDTLQCSGYRLDAIKHINHEFIKEF 240 NVDDEFGNYDYLMFANIDY+ +VRREM++WGKWL+DTLQC G+RLDAIKHINHEFIKEF Sbjct: 187 NVDDEFGNYDYLMFANIDYNHPDVRREMIDWGKWLIDTLQCGGFRLDAIKHINHEFIKEF 246 Query: 241 AAEMTKKRGEDFYIVGEFWNSNLEACREFLNTVDYQIDLFDVSLHYKLYAAALGGRDFDL 300 AAEM +KRG+DFYIVGEFWNSNL+ACREFL+TVDYQIDLFDVSLHYKL+ A+L GRDFDL Sbjct: 247 AAEMIRKRGQDFYIVGEFWNSNLDACREFLDTVDYQIDLFDVSLHYKLHEASLKGRDFDL 306 Query: 301 THIFDDTLVQTHPGNAVTFVDNHDSQPHEALESWVGDWFKQSAYALILLRRDGYPVVFYG 360 + IFDDTLVQTHP +AVTFVDNHDSQPHEALESW+GDWFK SAYAL LLRRDGYPVVFYG Sbjct: 307 SKIFDDTLVQTHPTHAVTFVDNHDSQPHEALESWIGDWFKPSAYALTLLRRDGYPVVFYG 366 Query: 361 DYYGIGGPAPIEGKKAAIDPLLYARCHKAYGEQDDYFDHPNTIGWVRRGIEEIEGSGCAV 420 DYYGIGGP P++GKK +D LL ARC+KAYGEQ+DYFDH NTIGWVRRG+EEIEGSGCAV Sbjct: 367 DYYGIGGPEPVDGKKEILDILLSARCNKAYGEQEDYFDHANTIGWVRRGVEEIEGSGCAV 426 Query: 421 VISNGDNGEKRMFLGEERAGEVWVDFTHNRRESVTIGKDGWAVFPVNGGSVSVWALPDGA 480 VISNGD+GEKRMF+GE RAGEVWVD T + + +TI +DGWA F V GG VSVWALP+ Sbjct: 427 VISNGDDGEKRMFIGEHRAGEVWVDLTKSCDDQITIEEDGWATFHVCGGGVSVWALPEQN 486 Query: 481 E 481 E Sbjct: 487 E 487 >ref|ZP_00183205.1| hypothetical protein Exigu144301 [Exiguobacterium sp. 255-15] Length = 486 Score = 671 bits (1732), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/478 (65%), Positives = 375/478 (78%) Query: 1 MMQFFEWHVAADGQHWKRLAQMAPELKAAGIDSVWVPPVTKAISPEDTGYGVYDLYDLGE 60 MMQFFEWHV DG+HW+RL + APEL+AAGI SVW+PP +K S EDTGYG+YD+YDLGE Sbjct: 7 MMQFFEWHVENDGKHWQRLKERAPELRAAGITSVWIPPASKGQSDEDTGYGIYDVYDLGE 66 Query: 61 FDQKGTIRTKYGTKQELIDAIAECLRNGIAVYVDLVMNHKAGADETEVFEVIEVDPNNRN 120 FDQKGT+RTKYGTK ELI+AI N IAVY D+VMNHKA ADE E V+EV P +R+ Sbjct: 67 FDQKGTVRTKYGTKDELIEAIQVAHENDIAVYADVVMNHKAAADELETINVVEVHPEDRS 126 Query: 121 KEISAPFEIEGWTKFTFPGRGDTYSSFKWNHTHFNGTDFDAKGGRNGVFRINGENKNWNQ 180 KEIS FEIE WTKFTFPGR YS F W H FNGTDFDA+ + GVF+I+G+NK+WN Sbjct: 127 KEISEEFEIEAWTKFTFPGRNGKYSDFIWTHEFFNGTDFDAREEKTGVFKISGKNKDWND 186 Query: 181 NVDDEFGNYDYLMFANIDYSQQEVRREMLEWGKWLVDTLQCSGYRLDAIKHINHEFIKEF 240 VD+EFGNYDYLMFANIDY+ EVR EM+ WG W +T+ C G+RLDAIKHIN+EF+ EF Sbjct: 187 QVDNEFGNYDYLMFANIDYNHPEVREEMINWGNWFKETINCQGFRLDAIKHINYEFVNEF 246 Query: 241 AAEMTKKRGEDFYIVGEFWNSNLEACREFLNTVDYQIDLFDVSLHYKLYAAALGGRDFDL 300 A M + FY+VGEFW S+L+ CR+FL++VDY IDLFDV LHYK + A+ G+DFDL Sbjct: 247 AKSMIGDDPDGFYMVGEFWKSDLDDCRQFLDSVDYTIDLFDVPLHYKFHEASQQGQDFDL 306 Query: 301 THIFDDTLVQTHPGNAVTFVDNHDSQPHEALESWVGDWFKQSAYALILLRRDGYPVVFYG 360 T +F DTLV++HP NAVTFVDNHDSQP E+LESW+ DWFKQ AYA +LLR+DGYP VFYG Sbjct: 307 TTLFADTLVESHPTNAVTFVDNHDSQPGESLESWIDDWFKQHAYASVLLRKDGYPCVFYG 366 Query: 361 DYYGIGGPAPIEGKKAAIDPLLYARCHKAYGEQDDYFDHPNTIGWVRRGIEEIEGSGCAV 420 DYYG+ GP P+EGKK ID LLY R HKAYGEQ+DY D + +GWVRRG+EE SGCAV Sbjct: 367 DYYGVQGPHPVEGKKEMIDALLYTRYHKAYGEQEDYMDDAHCVGWVRRGVEEFPNSGCAV 426 Query: 421 VISNGDNGEKRMFLGEERAGEVWVDFTHNRRESVTIGKDGWAVFPVNGGSVSVWALPD 478 ++SN D EKRMF+GE RAGE W D+T+++ E V I ++G+ VFPV GG VSV+A D Sbjct: 427 LLSNADMCEKRMFVGETRAGEEWFDYTNHQEEPVVIDEEGFGVFPVPGGGVSVFAPRD 484 >sp|P19571|AMT6_BACS7 Glucan 1,4-alpha-maltohexaosidase precursor (G6-amylase) (Maltohexaose-producing amylase) (Exo-maltohexaohydrolase) pir||A27705 alpha-amylase (EC 3.2.1.1) precursor - Bacillus sp gb|AAA22231.1| G6-amylase precursor Length = 518 Score = 513 bits (1321), Expect = e-144, Method: Compositional matrix adjust. Identities = 247/479 (51%), Positives = 321/479 (67%), Gaps = 8/479 (1%) Query: 1 MMQFFEWHVAADGQHWKRLAQMAPELKAAGIDSVWVPPVTKAISPEDTGYGVYDLYDLGE 60 MMQ+FEW++ DG HW RL A LK+ GI +VW+PP K S D GYG YDLYDLGE Sbjct: 42 MMQYFEWYLPNDGNHWNRLNSDASNLKSKGITAVWIPPAWKGASQNDVGYGAYDLYDLGE 101 Query: 61 FDQKGTIRTKYGTKQELIDAIAECLRNGIAVYVDLVMNHKAGADETEVFEVIEVDPNNRN 120 F+QKGT+RTKYGT+ +L A+ NGI VY D+VMNHK GAD TE+ +EV+PNNRN Sbjct: 102 FNQKGTVRTKYGTRSQLQAAVTSLKNNGIQVYGDVVMNHKGGADATEMVRAVEVNPNNRN 161 Query: 121 KEISAPFEIEGWTKFTFPGRGDTYSSFKWNHTHFNGTDFD-AKGGRNGVFRINGENKNWN 179 +E++ + IE WT+F FPGRG+T+SSFKW HF+G D+D ++ N +++ G K W+ Sbjct: 162 QEVTGEYTIEAWTRFDFPGRGNTHSSFKWRWYHFDGVDWDQSRRLNNRIYKFRGHGKAWD 221 Query: 180 QNVDDEFGNYDYLMFANIDYSQQEVRREMLEWGKWLVDTLQCSGYRLDAIKHINHEFIKE 239 VD E GNYDYLM+A+ID EV E+ WG W +TL G+R+DA+KHI + F ++ Sbjct: 222 WEVDTENGNYDYLMYADIDMDHPEVVNELRNWGVWYTNTLGLDGFRIDAVKHIKYSFTRD 281 Query: 240 FAAEMTKKRGEDFYIVGEFWNSNLEACREFLNTVDYQIDLFDVSLHYKLYAAALGGRDFD 299 + + G++ + V EFW ++L A +L ++ +FDV LHY LY A+ G ++D Sbjct: 282 WINHVRSATGKNMFAVAEFWKNDLGAIENYLQKTNWNHSVFDVPLHYNLYNASKSGGNYD 341 Query: 300 LTHIFDDTLVQTHPGNAVTFVDNHDSQPHEALESWVGDWFKQSAYALILLRRDGYPVVFY 359 + +IF+ T+VQ HP +AVTFVDNHDSQP EALES+V +WFK AYAL L R GYP VFY Sbjct: 342 MRNIFNGTVVQRHPSHAVTFVDNHDSQPEEALESFVEEWFKPLAYALTLTREQGYPSVFY 401 Query: 360 GDYYGI---GGPAPIEGKKAAIDPLLYARCHKAYGEQDDYFDHPNTIGWVRRGIEEIEGS 416 GDYYGI G PA ++ IDP+L AR AYG+Q+DY DH N IGW R G S Sbjct: 402 GDYYGIPTHGVPA----MRSKIDPILEARQKYAYGKQNDYLDHHNIIGWTREGNTAHPNS 457 Query: 417 GCAVVISNGDNGEKRMFLGEERAGEVWVDFTHNRRESVTIGKDGWAVFPVNGGSVSVWA 475 G A ++S+G G K MF+G +AG+VW D T NR +VTI DGW F VNGGSVS+W Sbjct: 458 GLATIMSDGAGGSKWMFVGRNKAGQVWSDITGNRTGTVTINADGWGNFSVNGGSVSIWV 516 >gb|AAF00567.1| raw starch digesting amylase precursor [Cytophaga sp.] Length = 519 Score = 513 bits (1321), Expect = e-144, Method: Compositional matrix adjust. Identities = 248/476 (52%), Positives = 311/476 (65%), Gaps = 2/476 (0%) Query: 1 MMQFFEWHVAADGQHWKRLAQMAPELKAAGIDSVWVPPVTKAISPEDTGYGVYDLYDLGE 60 MMQ+FEW+V DGQ W RL AP L + GI +VW PP K S D GYG YDLYDLGE Sbjct: 41 MMQYFEWYVPNDGQQWNRLRTDAPYLSSVGITAVWTPPAYKGTSQADVGYGPYDLYDLGE 100 Query: 61 FDQKGTIRTKYGTKQELIDAIAECLRNGIAVYVDLVMNHKAGADETEVFEVIEVDPNNRN 120 F+QKGT+RTKYGTK EL A+ NGI VY D+VMNHKAGAD TE +EV+P+NRN Sbjct: 101 FNQKGTVRTKYGTKGELKSAVNTLHSNGIQVYGDVVMNHKAGADYTENVTAVEVNPSNRN 160 Query: 121 KEISAPFEIEGWTKFTFPGRGDTYSSFKWNHTHFNGTDFDAKGGRNGVFRINGENKNWNQ 180 +E S + I+ WT F FPGRG TYS+FKW HF+GTD+D + +F+ G K W+ Sbjct: 161 QETSGEYNIQAWTGFNFPGRGTTYSNFKWQWFHFDGTDWDQSRSLSRIFKFRGTGKAWDW 220 Query: 181 NVDDEFGNYDYLMFANIDYSQQEVRREMLEWGKWLVDTLQCSGYRLDAIKHINHEFIKEF 240 V E GNYDYLM+A+IDY +V EM +WG W + + GYRLDA+KHI F+K++ Sbjct: 221 EVSSENGNYDYLMYADIDYDHPDVVNEMKKWGVWYANEVGLDGYRLDAVKHIKFSFLKDW 280 Query: 241 AAEMTKKRGEDFYIVGEFWNSNLEACREFLNTVDYQIDLFDVSLHYKLYAAALGGRDFDL 300 G++ + VGE+W ++L A +L V+Y LFD LHY YAA+ GG +D+ Sbjct: 281 VDNARAATGKEMFTVGEYWQNDLGALNNYLAKVNYNQSLFDAPLHYNFYAASTGGGYYDM 340 Query: 301 THIFDDTLVQTHPGNAVTFVDNHDSQPHEALESWVGDWFKQSAYALILLRRDGYPVVFYG 360 +I ++TLV ++P AVT V+NHD+QP ++LES V WFK AYA IL R GYP VFYG Sbjct: 341 RNILNNTLVASNPTKAVTLVENHDTQPGQSLESTVQPWFKPLAYAFILTRSGGYPSVFYG 400 Query: 361 DYYGIGGPAP--IEGKKAAIDPLLYARCHKAYGEQDDYFDHPNTIGWVRRGIEEIEGSGC 418 D YG G I K+ I+PLL AR AYG Q DY D+P+ IGW R G SG Sbjct: 401 DMYGTKGTTTREIPALKSKIEPLLKARKDYAYGTQRDYIDNPDVIGWTREGDSTKAKSGL 460 Query: 419 AVVISNGDNGEKRMFLGEERAGEVWVDFTHNRRESVTIGKDGWAVFPVNGGSVSVW 474 A VI++G G KRM++G AGE+W D T NR + +TIG DG+A FPVNGGSVSVW Sbjct: 461 ATVITDGPGGSKRMYVGTSNAGEIWYDLTGNRTDKITIGSDGYATFPVNGGSVSVW 516 >dbj|BAA32431.1| amylase [Bacillus sp.] Length = 516 Score = 508 bits (1308), Expect = e-142, Method: Compositional matrix adjust. Identities = 247/476 (51%), Positives = 317/476 (66%), Gaps = 2/476 (0%) Query: 1 MMQFFEWHVAADGQHWKRLAQMAPELKAAGIDSVWVPPVTKAISPEDTGYGVYDLYDLGE 60 MMQ+FEWH+ DG HW RL A LK+ GI +VW+PP K S D GYG YDLYDLGE Sbjct: 40 MMQYFEWHLPNDGNHWNRLRDDAANLKSKGITAVWIPPAWKGTSQNDVGYGAYDLYDLGE 99 Query: 61 FDQKGTIRTKYGTKQELIDAIAECLRNGIAVYVDLVMNHKAGADETEVFEVIEVDPNNRN 120 F+QKGT+RTKYGT+ +L A+ NGI VY D+VMNHK GAD TE+ +EV+ +NRN Sbjct: 100 FNQKGTVRTKYGTRSQLQGAVTSLKNNGIQVYGDVVMNHKGGADGTEMVNAVEVNRSNRN 159 Query: 121 KEISAPFEIEGWTKFTFPGRGDTYSSFKWNHTHFNGTDFD-AKGGRNGVFRINGENKNWN 179 +EIS + IE WTKF FPGRG+T+S+FKW HF+GTD+D ++ +N +++ G K W+ Sbjct: 160 QEISGEYTIEAWTKFDFPGRGNTHSNFKWRWYHFDGTDWDQSRQLQNKIYKFRGTGKAWD 219 Query: 180 QNVDDEFGNYDYLMFANIDYSQQEVRREMLEWGKWLVDTLQCSGYRLDAIKHINHEFIKE 239 VD E GNYDYLM+A+ID EV E+ WG W +TL G+R+DA+KHI + + ++ Sbjct: 220 WEVDIENGNYDYLMYADIDMDHPEVINELRNWGVWYTNTLNLDGFRIDAVKHIKYSYTRD 279 Query: 240 FAAEMTKKRGEDFYIVGEFWNSNLEACREFLNTVDYQIDLFDVSLHYKLYAAALGGRDFD 299 + + G+ + V EFW ++L A +LN + +FDV LHY LY A+ G FD Sbjct: 280 WLTHVRNTTGKPMFAVAEFWKNDLAAIENYLNKTSWNHSVFDVPLHYNLYNASNSGGYFD 339 Query: 300 LTHIFDDTLVQTHPGNAVTFVDNHDSQPHEALESWVGDWFKQSAYALILLRRDGYPVVFY 359 + +I + ++VQ HP +AVTFVDNHDSQP EALES+V WFK AYALIL R GYP VFY Sbjct: 340 MRNILNGSVVQKHPIHAVTFVDNHDSQPGEALESFVQSWFKPLAYALILTREQGYPSVFY 399 Query: 360 GDYYGIGGPAPIEGKKAAIDPLLYARCHKAYGEQDDYFDHPNTIGWVRRGIEEIEGSGCA 419 GDYYGI + K+ IDPLL AR AYG Q DYFDH + IGW R G SG A Sbjct: 400 GDYYGIPTHG-VPSMKSKIDPLLQARQTYAYGTQHDYFDHHDIIGWTREGDSSHPNSGLA 458 Query: 420 VVISNGDNGEKRMFLGEERAGEVWVDFTHNRRESVTIGKDGWAVFPVNGGSVSVWA 475 ++S+G G K M++G+ +AG+VW D T NR +VTI DGW F VNGG+VSVW Sbjct: 459 TIMSDGPGGNKWMYVGKHKAGQVWRDITGNRSGTVTINADGWGNFTVNGGAVSVWV 514 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.138 0.438 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 873,094,900 Number of extensions: 40868841 Number of successful extensions: 98052 Number of sequences better than 1.0e-05: 139 Number of HSP's gapped: 97994 Number of HSP's successfully gapped: 150 Length of query: 505 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 375 Effective length of database: 404,274,708 Effective search space: 151603015500 Effective search space used: 151603015500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)