BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0176.0014.p (186 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_633900.1| Purine phosphoribosyltransferase [Methanosarcin... 80 2e-14 ref|ZP_00148012.1| hypothetical protein Mbur163301 [Methanococco... 76 4e-13 ref|NP_615677.1| hypoxanthine phosphoribosyltransferase [Methano... 75 6e-13 ref|ZP_00078349.1| COG0503: Adenine/guanine phosphoribosyltransf... 75 9e-13 ref|NP_279597.1| adenine phosphoribosyltransferase; Apt [Halobac... 71 9e-12 >ref|NP_633900.1| Purine phosphoribosyltransferase [Methanosarcina mazei Goe1] gb|AAM31572.1| Purine phosphoribosyltransferase [Methanosarcina mazei Goe1] Length = 189 Score = 80.5 bits (197), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 49/180 (27%), Positives = 96/180 (53%), Gaps = 10/180 (5%) Query: 7 RLSESISKAKNVRIYKDKDTAYDFKLYPFGERGTYIDPELISEITDSLAESITAQFPAFD 66 RL +S+ ++ + K Y++ ++P + IDP L+ EI+D ++E D Sbjct: 4 RLKDSLIRSPII-----KRGEYNYFIHPISDGVPSIDPHLVEEISDYISEIADMNV---D 55 Query: 67 YIVSPEPGGHTWGMLAAYKLMKPMNILRLSTELYDNYEVSIKRETAYNENYIYFDGFTAG 126 I++ E G + K P+ I+R + EV + + T Y++ +Y +G G Sbjct: 56 TILTVEAMGIPVANALSLKTGIPLTIVRKRPYFLEG-EVELSQSTGYSKGVLYINGLKKG 114 Query: 127 DRVLLLDDVISSGATIRCIARQMARMGIELTGVQAILAKGDHYKQLQTDIGVPVRFLSKV 186 DR++++DDVIS+G T+ + R + +G+E+ V +++ +GD Y +L+ ++GV + L + Sbjct: 115 DRIIIVDDVISTGGTLLALVRALQTIGVEVMDVISVIGRGDGYLKLR-ELGVEPKILVTI 173 >ref|ZP_00148012.1| hypothetical protein Mbur163301 [Methanococcoides burtonii DSM 6242] Length = 190 Score = 75.9 bits (185), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 47/159 (29%), Positives = 86/159 (54%), Gaps = 5/159 (3%) Query: 28 YDFKLYPFGERGTYIDPELISEITDSLAESITAQFPAFDYIVSPEPGGHTWGMLAAYKLM 87 Y + ++P + ++PEL+ EI D + I +D IVS E G + + K Sbjct: 20 YPYFIHPISDGIPSLEPELLDEIADHM---IDIAGKDYDRIVSIEAMGIPLATVLSMKTG 76 Query: 88 KPMNILRLSTELYDNYEVSIKRETAYNENYIYFDGFTAGDRVLLLDDVISSGATIRCIAR 147 KPM+I+R + EV + + T Y++ +Y +G + G+ VL++DDVIS+G T++ + Sbjct: 77 KPMSIVRKRQYGLEG-EVILSQSTGYSKGELYINGISKGETVLVVDDVISTGGTLKALLP 135 Query: 148 QMARMGIELTGVQAILAKGDHYKQLQTDIGVPVRFLSKV 186 + MG + V ++++GD ++ +D+G V L K+ Sbjct: 136 ALENMGANICNVIVVISRGDGAAKM-SDMGYNVNTLVKI 173 >ref|NP_615677.1| hypoxanthine phosphoribosyltransferase [Methanosarcina acetivorans str. C2A] gb|AAM04157.1| hypoxanthine phosphoribosyltransferase [Methanosarcina acetivorans str. C2A] Length = 189 Score = 75.5 bits (184), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 47/163 (28%), Positives = 86/163 (52%), Gaps = 5/163 (3%) Query: 24 KDTAYDFKLYPFGERGTYIDPELISEITDSLAESITAQFPAFDYIVSPEPGGHTWGMLAA 83 K Y++ ++P + IDP LI EI + + I D I++ E G + Sbjct: 16 KRGEYNYFIHPISDGVPSIDPRLIEEIANYI---IRIADMDVDTILTIEAMGIPVANALS 72 Query: 84 YKLMKPMNILRLSTELYDNYEVSIKRETAYNENYIYFDGFTAGDRVLLLDDVISSGATIR 143 K P+ I+R + EV + + T Y++ +Y +G GDRV+++DDVIS+G T+ Sbjct: 73 LKTGIPLTIVRKRPYFLEG-EVELSQSTGYSKGVLYINGLKKGDRVVIVDDVISTGGTLL 131 Query: 144 CIARQMARMGIELTGVQAILAKGDHYKQLQTDIGVPVRFLSKV 186 + + + MG+E+T V +++ +G Y +L+ ++GV + L + Sbjct: 132 ALVKALQNMGVEITDVISVIGRGAGYFKLR-ELGVEPKILVTI 173 >ref|ZP_00078349.1| COG0503: Adenine/guanine phosphoribosyltransferases and related PRPP-binding proteins [Methanosarcina barkeri] Length = 190 Score = 74.7 bits (182), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 43/163 (26%), Positives = 85/163 (52%), Gaps = 4/163 (2%) Query: 24 KDTAYDFKLYPFGERGTYIDPELISEITDSLAESITAQFPAFDYIVSPEPGGHTWGMLAA 83 K Y++ ++P + IDP ++ EI + + + D I++ E G + Sbjct: 16 KRGEYNYFIHPISDGVPSIDPHMVEEIAEYILQ--ITDIKKVDTILTIEAMGIPVANALS 73 Query: 84 YKLMKPMNILRLSTELYDNYEVSIKRETAYNENYIYFDGFTAGDRVLLLDDVISSGATIR 143 K P+ I+R + EV + + T Y++ +Y +G GDRV+++DDVIS+G T+ Sbjct: 74 LKTGIPLTIVRKRPYFLEG-EVELSQRTGYSKGALYINGLKKGDRVIIVDDVISTGGTLI 132 Query: 144 CIARQMARMGIELTGVQAILAKGDHYKQLQTDIGVPVRFLSKV 186 + + + +G+E+ V +++ +G Y QL+ ++GV + L + Sbjct: 133 ALVKALKTIGVEIQDVISVIGRGTGYLQLK-ELGVEPKILVTI 174 >ref|NP_279597.1| adenine phosphoribosyltransferase; Apt [Halobacterium sp. NRC-1] sp|Q9HRT1|APT_HALN1 Adenine phosphoribosyltransferase (APRT) pir||A84214 adenine phosphoribosyltransferase [imported] - Halobacterium sp. NRC-1 gb|AAG19077.1| adenine phosphoribosyltransferase; Apt [Halobacterium sp. NRC-1] Length = 188 Score = 71.2 bits (173), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 54/173 (31%), Positives = 88/173 (50%), Gaps = 10/173 (5%) Query: 7 RLSESISKAKNVRIYKDKDTAYDFKLYPFGERGTYIDPELISEITDSLAESITAQFPAFD 66 RL +S+ A I KD Y + ++P + ++PEL+ EI + A+ D Sbjct: 3 RLKQSLLDAP--IIEKD---GYHYFVHPISDGVPMLEPELLREIVIRIIRK--AELDEVD 55 Query: 67 YIVSPEPGGHTWGMLAAYKLMKPMNILRLSTELYD-NYEVSIKRETAYNENYIYFDGFTA 125 IV+P G + A LM + I+ + Y EV++ +ET Y+EN +Y + Sbjct: 56 KIVTPAAMG--IHISTAVSLMTDIPIVVIRKREYGLPGEVALSQETGYSENEMYINDVHE 113 Query: 126 GDRVLLLDDVISSGATIRCIARQMARMGIELTGVQAILAKGDHYKQLQTDIGV 178 GDRVL+LDDV+S+G T+R I + + G ++ V A++ K + TD+ V Sbjct: 114 GDRVLVLDDVLSTGGTLRAITDALEQTGADVADVLAVIKKAGPNELDDTDMDV 166 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 263,127,319 Number of extensions: 10268050 Number of successful extensions: 29046 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 29219 Number of HSP's successfully gapped: 14 Length of query: 186 Length of database: 757,237,578 Length adjustment: 118 Effective length of query: 68 Effective length of database: 436,855,896 Effective search space: 29706200928 Effective search space used: 29706200928 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 122 (51.6 bits)