BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0176.0010.p (463 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value sp|O69108|HEM1_PAEMA Glutamyl-tRNA reductase (GluTR) >gi|3172403... 665 0.0 ref|NP_243914.1| glutamyl-tRNA reductase [Bacillus halodurans] >... 498 e-139 sp|Q8GCB0|HEM1_BACME Glutamyl-tRNA reductase (GluTR) >gi|2711722... 494 e-138 ref|NP_390695.1| glutamyl-tRNA reductase [Bacillus subtilis] >gi... 469 e-131 ref|NP_980850.1| glutamyl-tRNA reductase [Bacillus cereus ATCC 1... 466 e-130 >sp|O69108|HEM1_PAEMA Glutamyl-tRNA reductase (GluTR) gb|AAC18585.1| glutamyl-tRNA reductase [Paenibacillus macerans] Length = 437 Score = 665 bits (1717), Expect = 0.0, Method: Compositional matrix adjust. Identities = 317/423 (74%), Positives = 366/423 (86%) Query: 1 MHIVVVGLNYRTAPVEVREQFAFAEKDLPAALHQLMQTKSVLEGVVVATCNRTEIYVVVD 60 MHIVVVGLNYRTAPVEVRE+F+FAEKD+P AL +L++TKSVLEGV++ATCNRTEIYVVVD Sbjct: 1 MHIVVVGLNYRTAPVEVRERFSFAEKDMPQALQELLRTKSVLEGVIIATCNRTEIYVVVD 60 Query: 61 RLHMCGYFIRSYMEQWFGVKSDVFAQHMYIYEDEQAIAHLFRVTCGLDSMVIGETQILGQ 120 RLHMCGYFIRS+ME+WFGV + F +H+Y+YEDEQA+ HLFRV CGLDSMV+GETQILGQ Sbjct: 61 RLHMCGYFIRSFMERWFGVPREEFTRHLYMYEDEQAVRHLFRVACGLDSMVLGETQILGQ 120 Query: 121 VRSAFLTAQAEGVTGTWFNRLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGMF 180 V+SAFL +Q + TGTWFN LFKQAVTLGKRAHSET+IG+SAVS+SYAAVELGKRIFG F Sbjct: 121 VKSAFLLSQRQKGTGTWFNMLFKQAVTLGKRAHSETAIGQSAVSISYAAVELGKRIFGSF 180 Query: 181 TGKRVLILGAGKMSELTVKHLYSSGAAEVIVANRTLARAAELAGKFSGKPSTIEHALQHL 240 +GKRVLILGAGKMSELT KHL GA EVIVANRT ARA ELA KF G P T++ A++ L Sbjct: 181 SGKRVLILGAGKMSELTAKHLSGGGADEVIVANRTYARAQELAAKFDGTPCTMQEAMERL 240 Query: 241 DEVDIVISSTGADGYVLTADQVADNMKRRPSRPLFMIDIAVPRDIDPAAASLPDVFLYDI 300 +VDI+ISSTGA+G QV +MKRRP RPLFMIDIAVPRDI+P L +VFLYDI Sbjct: 241 ADVDILISSTGAEGIRDHVGQVERSMKRRPDRPLFMIDIAVPRDIEPEIGVLENVFLYDI 300 Query: 301 DDLEGIVETNLEMRRSEAAKIEVMIEAELEDFQMWLKTLGVRPVIRALQDKSNGIYEDTM 360 DDLEGIVE NLEMRR+EA KI+ MIE E++ F WL+TLGV+PVIRALQ+K+ I+E T+ Sbjct: 301 DDLEGIVENNLEMRRAEAVKIDKMIEEEMQVFANWLQTLGVKPVIRALQEKAAHIHESTL 360 Query: 361 DSLFNKLPELDEHQRKVIRRLTKSIVNQMMHDPINVIKELSGGKQGNEALEYFTQIFALQ 420 DS+FNKLPELDE QRKVIRRLTKSI+NQMMHDPIN IKE++GGKQG EALE FTQIFAL+ Sbjct: 361 DSMFNKLPELDERQRKVIRRLTKSILNQMMHDPINRIKEMAGGKQGAEALEMFTQIFALE 420 Query: 421 EQL 423 + L Sbjct: 421 KHL 423 >ref|NP_243914.1| glutamyl-tRNA reductase [Bacillus halodurans] sp|Q9K8F8|HEM1_BACHD Glutamyl-tRNA reductase (GluTR) pir||H84030 glutamyl-tRNA reductase hemA [imported] - Bacillus halodurans (strain C-125) dbj|BAB06767.1| glutamyl-tRNA reductase [Bacillus halodurans] Length = 459 Score = 498 bits (1282), Expect = e-139, Method: Compositional matrix adjust. Identities = 247/417 (59%), Positives = 320/417 (76%) Query: 1 MHIVVVGLNYRTAPVEVREQFAFAEKDLPAALHQLMQTKSVLEGVVVATCNRTEIYVVVD 60 MHI+++GLNY+TAPVE+RE+F+F + +LP ALHQL Q KS+LE +V+TCNRTE+YVV D Sbjct: 1 MHILMIGLNYKTAPVEIREKFSFQDSELPQALHQLRQMKSILECTIVSTCNRTELYVVAD 60 Query: 61 RLHMCGYFIRSYMEQWFGVKSDVFAQHMYIYEDEQAIAHLFRVTCGLDSMVIGETQILGQ 120 +LH +F ++++ WF + D F + I E++ AI HLFRV GLDSM++GETQILGQ Sbjct: 61 QLHTGRHFTKTFLADWFKLPKDEFTPFLTIRENDHAIEHLFRVVTGLDSMILGETQILGQ 120 Query: 121 VRSAFLTAQAEGVTGTWFNRLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGMF 180 VR++F AQ E VTG+ FN LFKQA+TL KRAHSET IG++AVSVSYAAVELGK+IF F Sbjct: 121 VRNSFFIAQEEQVTGSIFNHLFKQAITLAKRAHSETDIGQNAVSVSYAAVELGKKIFDDF 180 Query: 181 TGKRVLILGAGKMSELTVKHLYSSGAAEVIVANRTLARAAELAGKFSGKPSTIEHALQHL 240 GK+VLILGAGKM ELT KHL+S+GA +V V NRT +AAELA +F G + + Sbjct: 181 KGKQVLILGAGKMGELTAKHLHSNGAEQVTVINRTREKAAELAKRFLGVDRPYNELTEAI 240 Query: 241 DEVDIVISSTGADGYVLTADQVADNMKRRPSRPLFMIDIAVPRDIDPAAASLPDVFLYDI 300 E DI+ISSTGA GYV+T+D V+ +K+R RPLFM+DIAVPRD+DPA AS DV+LYDI Sbjct: 241 VEADILISSTGATGYVVTSDMVSHALKKRKGRPLFMVDIAVPRDLDPALASHDDVYLYDI 300 Query: 301 DDLEGIVETNLEMRRSEAAKIEVMIEAELEDFQMWLKTLGVRPVIRALQDKSNGIYEDTM 360 DDL+ IV+TNLE RR+EA KIE++IE EL +F+ WL TLGV P+I AL+ K+ + +TM Sbjct: 301 DDLQNIVQTNLEERRTEAEKIELLIEEELVEFKQWLNTLGVVPIITALRTKALTVQGETM 360 Query: 361 DSLFNKLPELDEHQRKVIRRLTKSIVNQMMHDPINVIKELSGGKQGNEALEYFTQIF 417 +S+ KLP L E ++KV+R+ TKSIVNQ++ DPI IKEL+ + EAL+ FT+IF Sbjct: 361 ESIERKLPNLTEREKKVLRKHTKSIVNQLLRDPITRIKELANAPEREEALDLFTKIF 417 >sp|Q8GCB0|HEM1_BACME Glutamyl-tRNA reductase (GluTR) emb|CAD48144.1| glutamyl-tRNA-reductase [Bacillus megaterium] Length = 446 Score = 494 bits (1273), Expect = e-138, Method: Compositional matrix adjust. Identities = 240/427 (56%), Positives = 320/427 (74%), Gaps = 6/427 (1%) Query: 1 MHIVVVGLNYRTAPVEVREQFAFAEKDLPAALHQLMQTKSVLEGVVVATCNRTEIYVVVD 60 MHI+ VGLN+RTAPVE+RE+ +F E++L +A+ L KS+LE ++V+TCNRTEIY VVD Sbjct: 1 MHIIAVGLNFRTAPVEIREKLSFNEQELASAMKTLSGQKSILENIIVSTCNRTEIYAVVD 60 Query: 61 RLHMCGYFIRSYMEQWFGVKSDVFAQHMYIYEDEQAIAHLFRVTCGLDSMVIGETQILGQ 120 +LH Y++++++ +WFG+ + F+ ++ IYE++ AI HL+RV CGLDSMVIGETQILGQ Sbjct: 61 QLHTGRYYVKAFLAEWFGIDKEEFSPYLTIYENDGAIEHLYRVACGLDSMVIGETQILGQ 120 Query: 121 VRSAFLTAQAEGVTGTWFNRLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGMF 180 VRS+FL AQ E GT FN+LFKQAVTL K+AH ET IG +AVSVSYAAVEL K+IFG Sbjct: 121 VRSSFLLAQEEETIGTVFNQLFKQAVTLAKKAHHETEIGANAVSVSYAAVELAKKIFGDL 180 Query: 181 TGKRVLILGAGKMSELTVKHLYSSGAAEVIVANRTLARAAELAGKFSG--KP-STIEHAL 237 + K VLILGAGKM +L V++LY SGA +V V NRT +A ELA +FSG KP + ++HA Sbjct: 181 SSKHVLILGAGKMGQLAVQNLYGSGAKKVTVVNRTFEKAQELANRFSGEAKPFADLQHA- 239 Query: 238 QHLDEVDIVISSTGADGYVLTADQVADNMKRRPSRPLFMIDIAVPRDIDPAAASLPDVFL 297 L E DI+ISSTGA+ YV+T +++ + R RPLFM+DIAVPRD+DP L VFL Sbjct: 240 --LSEADILISSTGANDYVVTKQMMSEAERTRKGRPLFMVDIAVPRDLDPELDELETVFL 297 Query: 298 YDIDDLEGIVETNLEMRRSEAAKIEVMIEAELEDFQMWLKTLGVRPVIRALQDKSNGIYE 357 YDIDDL GIVE+NL+ R+ A +IE+M+EAE+ F+ WL TLGV PVI AL+ K+ I Sbjct: 298 YDIDDLNGIVESNLQERQKAADEIEIMLEAEIVAFKSWLGTLGVVPVISALRQKALTIQA 357 Query: 358 DTMDSLFNKLPELDEHQRKVIRRLTKSIVNQMMHDPINVIKELSGGKQGNEALEYFTQIF 417 +TM S+ KLP+L E +RKV+ + TKSI+NQ++ DPI KEL+G K E+LE F +IF Sbjct: 358 ETMKSIDRKLPDLSERERKVLNKHTKSIINQLLRDPIPHAKELAGEKHAEESLELFMKIF 417 Query: 418 ALQEQLG 424 +++++ Sbjct: 418 NIEQEVA 424 >ref|NP_390695.1| glutamyl-tRNA reductase [Bacillus subtilis] sp|P16618|HEM1_BACSU Glutamyl-tRNA reductase (GluTR) pir||A35252 glutamyl-tRNA reductase (EC 1.2.1.-) hemA - Bacillus subtilis gb|AAA22510.1| NAD(P)H:glutamyl-transfer RNA reductase emb|CAA99543.1| NAD(P)H:glutamyl-transfer RNA reductase [Bacillus subtilis] emb|CAB14777.1| glutamyl-tRNA reductase [Bacillus subtilis subsp. subtilis str. 168] Length = 455 Score = 469 bits (1206), Expect = e-131, Method: Compositional matrix adjust. Identities = 223/424 (52%), Positives = 300/424 (70%) Query: 1 MHIVVVGLNYRTAPVEVREQFAFAEKDLPAALHQLMQTKSVLEGVVVATCNRTEIYVVVD 60 MHI+VVG++Y++AP+E+RE+ +F +L A+ QL + KS+LE ++V+TCNRTEIY VVD Sbjct: 1 MHILVVGVDYKSAPIEIREKVSFQPNELAEAMVQLKEEKSILENIIVSTCNRTEIYAVVD 60 Query: 61 RLHMCGYFIRSYMEQWFGVKSDVFAQHMYIYEDEQAIAHLFRVTCGLDSMVIGETQILGQ 120 +LH Y+I+ ++ WF + + + + YE + A+ HLFRV CGLDSMVIGETQILGQ Sbjct: 61 QLHTGRYYIKKFLADWFQLSKEELSPFLTFYESDAAVEHLFRVACGLDSMVIGETQILGQ 120 Query: 121 VRSAFLTAQAEGVTGTWFNRLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGMF 180 VR +F TAQ E GT FN LFKQAVT+GKR H+ET IG +AVSVSYAAVEL K+IFG Sbjct: 121 VRDSFKTAQQEKTIGTIFNELFKQAVTVGKRTHAETDIGSNAVSVSYAAVELAKKIFGNL 180 Query: 181 TGKRVLILGAGKMSELTVKHLYSSGAAEVIVANRTLARAAELAGKFSGKPSTIEHALQHL 240 + K +LILGAGKM EL ++L+ G +V V NRT +A ELA +FSG+ ++ L Sbjct: 181 SSKHILILGAGKMGELAAENLHGQGIGKVTVINRTYLKAKELADRFSGEARSLNQLESAL 240 Query: 241 DEVDIVISSTGADGYVLTADQVADNMKRRPSRPLFMIDIAVPRDIDPAAASLPDVFLYDI 300 E DI+ISSTGA +V++ + + + K R RPLFM+DIAVPRD+DPA L VFLYDI Sbjct: 241 AEADILISSTGASEFVVSKEMMENANKLRKGRPLFMVDIAVPRDLDPALNDLEGVFLYDI 300 Query: 301 DDLEGIVETNLEMRRSEAAKIEVMIEAELEDFQMWLKTLGVRPVIRALQDKSNGIYEDTM 360 DDLEGIVE N++ RR A K+E++IE + +F+ W+ TLGV PVI AL++K+ I +TM Sbjct: 301 DDLEGIVEANMKERRETAEKVELLIEETIVEFKQWMNTLGVVPVISALREKALAIQSETM 360 Query: 361 DSLFNKLPELDEHQRKVIRRLTKSIVNQMMHDPINVIKELSGGKQGNEALEYFTQIFALQ 420 DS+ KLP L ++K++ + TKSI+NQM+ DPI +KEL+ E L F QIF ++ Sbjct: 361 DSIERKLPHLSTREKKLLNKHTKSIINQMLRDPILKVKELAADADSEEKLALFMQIFDIE 420 Query: 421 EQLG 424 E G Sbjct: 421 EAAG 424 >ref|NP_980850.1| glutamyl-tRNA reductase [Bacillus cereus ATCC 10987] gb|AAS43458.1| glutamyl-tRNA reductase [Bacillus cereus ATCC 10987] Length = 444 Score = 466 bits (1198), Expect = e-130, Method: Compositional matrix adjust. Identities = 233/420 (55%), Positives = 298/420 (70%) Query: 1 MHIVVVGLNYRTAPVEVREQFAFAEKDLPAALHQLMQTKSVLEGVVVATCNRTEIYVVVD 60 MHI+VV +NYRTAPVE RE+ F +L A+ L + KSVLE V+V+TCNRTEIY VVD Sbjct: 1 MHILVVSVNYRTAPVEFREKLTFQATELEQAMTTLQKQKSVLENVIVSTCNRTEIYAVVD 60 Query: 61 RLHMCGYFIRSYMEQWFGVKSDVFAQHMYIYEDEQAIAHLFRVTCGLDSMVIGETQILGQ 120 +LH Y+I+ ++ WF ++ + A ++ I+E + AI HLFRVTCGLDSMV+GETQILGQ Sbjct: 61 QLHTGRYYIKKFLADWFQLEIEEVAPYLTIFEQDGAIDHLFRVTCGLDSMVVGETQILGQ 120 Query: 121 VRSAFLTAQAEGVTGTWFNRLFKQAVTLGKRAHSETSIGESAVSVSYAAVELGKRIFGMF 180 ++ +FL AQ TGT FN LFKQ +TL KRAHSET+IGESA+SVSYAAVELGK+IFG Sbjct: 121 IKDSFLEAQQVKATGTIFNELFKQVITLAKRAHSETTIGESAMSVSYAAVELGKKIFGEL 180 Query: 181 TGKRVLILGAGKMSELTVKHLYSSGAAEVIVANRTLARAAELAGKFSGKPSTIEHALQHL 240 T VLILGAGKM EL +++LY SGA +V V NRTL++A +A K+ G + L Sbjct: 181 TDCHVLILGAGKMGELALQNLYGSGARKVTVMNRTLSKAEIMAEKYMGHAKPLSELQCAL 240 Query: 241 DEVDIVISSTGADGYVLTADQVADNMKRRPSRPLFMIDIAVPRDIDPAAASLPDVFLYDI 300 E DI+ISSTGA YV+T + + K R RPLFM+DIAVPRDIDPA L FLYDI Sbjct: 241 LEADILISSTGASDYVITKEMMTKVEKMRSGRPLFMVDIAVPRDIDPAIDELEGSFLYDI 300 Query: 301 DDLEGIVETNLEMRRSEAAKIEVMIEAELEDFQMWLKTLGVRPVIRALQDKSNGIYEDTM 360 DDL+G+VE N R EA KI+ MIE E+ F+ WL TLGV P+I AL+DK+ I +TM Sbjct: 301 DDLQGVVEANRAERLKEAEKIQFMIEEEIVLFKTWLSTLGVVPLISALRDKALAIQSETM 360 Query: 361 DSLFNKLPELDEHQRKVIRRLTKSIVNQMMHDPINVIKELSGGKQGNEALEYFTQIFALQ 420 +SL K+P L + +RKVI + TKSI+NQ++ DPI V KE++ + +E L F +IF L+ Sbjct: 361 ESLERKIPTLSDRERKVISKHTKSIINQLLKDPILVAKEIAAEEGADEKLALFAKIFDLE 420 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.135 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 607,246,147 Number of extensions: 23633676 Number of successful extensions: 69623 Number of sequences better than 1.0e-05: 345 Number of HSP's gapped: 69085 Number of HSP's successfully gapped: 347 Length of query: 463 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 334 Effective length of database: 406,989,807 Effective search space: 135934595538 Effective search space used: 135934595538 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)